Origin IGS: gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcatctatcgtaaaataaaaatggctaattttacttgttttagtacaaaaaggcgacagaaatgtaagccgaatcgcggttcaacgcacctaaatagggcgacggctttcaatttcagtggaatcgtatataatgtgtgccgttttttagccgttaaaaagcaacctaatattaatcttaacaagaggaaaaatc Mask Tandem Repeat Region ================================================ gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Find is-nt database================================================ Query_seq: AA00396:AA00397|AA00396:AA00397:conserved hypothetical protein:Fe-S oxidoreductase; MiaB-like RNA modifying enzyme (2-methylthioadenine synthetase):->->:266013..266208 196 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00396:AA00397|AA00396:AA00397:conserved hypothetical protein:Fe-S oxidoreductase; MiaB-like RNA modifying enzyme (2-methylthioadenine synthetase):->->:266013..266208 196 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00396:AA00397|AA00396:AA00397:conserved hypothetical protein:Fe-S oxidoreductase; MiaB-like RNA modifying enzyme (2-methylthioadenine synthetase):->->:266013..266208 196 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 12 End: 188 ........... M L R L I L G C F L T A K K R H T L Y T I P L K L K A V A L F R C V E P R F G L H F C R L F V L K Q V K L A I F I L R ........ Protein_Len: 60 Strand: - Start: 2 End: 196 . L N I N P Q K K V A L F R C V N Y V I G S F N F A T A R N L H T S G R N P K C K Q R R K T S F C T F N A M K I K R Y I M gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00396:AA00397|AA00396:AA00397:conserved hypothetical protein:Fe-S oxidoreductase; MiaB-like RNA modifying enzyme (2-methylthioadenine synthetase):->->:266013..266208 196 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Intra-Species Hit: Count: 1 Min: 1 Max: 196 Len: 196 Subject: aact_AA00396_AA00397|conserved hypothetical protein:Fe-S oxidoreductase; MiaB-like RNA modifying enzyme (2-methylthioadenine synthetase)|POSITIVE:POSITIVE|[266013,266208]|196 HSP 1 e-value: 1.0E-107 bit: 389.0 Len: 196 Query Start:1 Query End:196 Subject Strand: POSITIVE Subject Start: 1 Subject End: 196 gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat gatttttcctcttgttaagattaatattaggttgctttttaacggctaaaaaacggcacacattatatacgattccactgaaattgaaagccgtcgccctatttaggtgcgttgaaccgcgattcggcttacatttctgtcgcctttttgtactaaaacaagtaaaattagccatttttattttacgatagatgat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUUUUCCUCUUGUUAAGAUUAAUAUUAGGUUGCUUUUUAACGGCUAAAAAACGGCACACAUUAUAUACGAUUCCACUGAAAUUGAAAGCCGUCGCCCUAUUUAGGUGCGUUGAACCGCGAUUCGGCUUACAUUUCUGUCGCCUUUUUGUACUAAAACAAGUAAAAUUAGCCAUUUUUAUUUUACGAUAGAUGAU .......(((..(((((....)))))..)))............((((((.....(((((...........(....)...(((((.(.((((((((((........(((.......)))))))..)))))).)))))).)).)))..(((((......))))).....))))))....((((((......)))))). (-35.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAUCUAUCGUAAAAUAAAAAUGGCUAAUUUUACUUGUUUUAGUACAAAAAGGCGACAGAAAUGUAAGCCGAAUCGCGGUUCAACGCACCUAAAUAGGGCGACGGCUUUCAAUUUCAGUGGAAUCGUAUAUAAUGUGUGCCGUUUUUUAGCCGUUAAAAAGCAACCUAAUAUUAAUCUUAACAAGAGGAAAAAUC ....................(((((((((...((((......))))......((((((((((((..((((((....(((......))).(((....)))....))))))...))))).(((........)))..))).)))).....))))))))).........(((....(((....)))....)))....... (-38.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 413 131 91 185 144 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 371 130 83 161 124 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 366 150 105 63 11 aact:AA00927|5end_ATP-dependent_DNA_helicase_635811..636041_POSITIVE 357 139 95 188 131 aact:AA00507|5end_hypothetical_protein_352996..353226_POSITIVE 356 144 109 44 12 aact:AA00950|5end_conserved_hypothetical_protein_656922..657152_POSITIVE 340 130 84 175 136 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 337 130 90 42 8 aact:AA00471|5end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328262..328492_POSITIVE 333 156 119 215 166 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 331 129 82 113 60 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 329 98 53 129 86 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 326 140 98 211 163 aact:AA02649|5end_possible_glycosyltransferase_1845562..1845792_POSITIVE 326 145 105 79 36 aact:AA02544|5end_ATP-dependent_RNA_helicase_1773317..1773547_POSITIVE 326 130 91 180 134 aact:AA01553|5end_conserved_hypothetical_protein_1039220..1039450_POSITIVE 326 127 84 77 42 aact:AA00450|5end_outer_membrane_antigenic_lipoprotein_b_precursor_313820..314050_POSITIVE 325 133 91 72 18 aact:AA01411|5end_stationary-phase_survival_protein_951000..951230_POSITIVE 324 140 91 55 12 aact:AA01499|5end_fructose_1,6-bisphosphatase_protein_1008357..1008587_POSITIVE 322 140 92 66 10 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 322 128 86 226 164 aact:AA00561|5end_DNA_transformation_protein_384584..384814_POSITIVE 322 145 101 191 143 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 321 128 89 151 97 aact:AA01645|5end_ferric_transport_ABC-related_ATP-binding_protein_1114016..1114246_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 130 83 127 90 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 366 150 105 63 11 aact:AA00926|3end_CyaY_protein_635891..636041_POSITIVE 334 144 105 130 82 aact:AA00747|3end_UTP--hexose-1-phosphate_uridylyltransferase_/_UDPglucose-hexose-1-phosphate_uridylyltransferas_513504..513654_POSITIVE 326 145 105 116 73 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 326 127 84 86 51 aact:AA00449|3end_conserved_hypothetical_protein_313909..314059_POSITIVE 323 129 91 151 111 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 320 144 106 130 94 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 313 130 81 82 32 aact:AA01579|3end_transcriptional_regulatory_protein_1065283..1065433_POSITIVE 313 99 53 86 33 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 306 99 55 150 91 aact:AA00760|3end_conserved_hypothetical_protein_(possible_peptidase)_523815..523965_POSITIVE 304 130 85 142 99 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 300 128 81 83 31 aact:AA01731|3end_multidrug_resistance_protein_B_1170675..1170825_POSITIVE 300 130 85 89 30 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 299 128 89 136 93 aact:AA00426|3end_conserved_hypothetical_protein_288205..288355_POSITIVE 299 150 108 58 11 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 297 127 85 129 63 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 297 130 89 64 27 aact:AA00979|3end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_685054..685204_POSITIVE 294 129 90 144 98 aact:AA00682|3end_S-adenosylmethionine-dependent_methyltransferase_467475..467625_POSITIVE 294 129 88 141 97 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 291 138 94 85 35 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE