Origin IGS: tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttggtcgtgccaaaacttaacagtaaacccgacattgtgtcgggtttactttatggcgcaaaaagtgcggttatttttcaaggtgttttttcgtgggtggtgttca Mask Tandem Repeat Region ================================================ tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Find is-nt database================================================ Query_seq: AA00391:AA00395|AA00391:AA00395:arsenate reductase:conserved hypothetical protein:->->:265309..265417 109 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00391:AA00395|AA00391:AA00395:arsenate reductase:conserved hypothetical protein:->->:265309..265417 109 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00391:AA00395|AA00391:AA00395:arsenate reductase:conserved hypothetical protein:->->:265309..265417 109 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 2 End: 106 . F V V W S F V G Q F I V A S K A G Y L L G S V I D P K S N L K P V V M ... tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -14.6 Tail_Score: -4.07568 Start: 52 End: 91 Full_Region: CCGCACTTTTTGCGC CATAAAGTAAACCCGACA CAA TGTCGGGTTTACTGTTAAG TTTTGGCACGACCAA ...................................................CATAAAGTAAACCCGACACAATGTCGGGTTTACTGTTAAG.................. TransTerm Strand: + Conf: 100 HP_score: -15.6 Tail_Score: -3.36823 Start: 56 End: 86 Full_Region: ACTTTTTGCGCCATA AAGTAAACCCGACA CAA TGTCGGGTTTACTG TTAAGTTTTGGCACG .......................................................AAGTAAACCCGACACAATGTCGGGTTTACTG....................... TransTerm Strand: + Conf: 100 HP_score: -18.2 Tail_Score: -3.25113 Start: 58 End: 84 Full_Region: TTTTTGCGCCATAAA GTAAACCCGACA CAA TGTCGGGTTTAC TGTTAAGTTTTGGCA .........................................................GTAAACCCGACACAATGTCGGGTTTAC......................... Find igs database================================================ Query_seq: AA00391:AA00395|AA00391:AA00395:arsenate reductase:conserved hypothetical protein:->->:265309..265417 109 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: aact_AA00391_AA00395|arsenate reductase:conserved hypothetical protein|POSITIVE:POSITIVE|[265309,265417]|109 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta tgaacaccacccacgaaaaaacaccttgaaaaataaccgcactttttgcgccataaagtaaacccgacacaatgtcgggtttactgttaagttttggcacgaccaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAACACCACCCACGAAAAAACACCUUGAAAAAUAACCGCACUUUUUGCGCCAUAAAGUAAACCCGACACAAUGUCGGGUUUACUGUUAAGUUUUGGCACGACCAACUA ..............(..(((((...............((((.....))))......((((((((((((.....)))))))))))).....)))))..)........... (-27.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUGGUCGUGCCAAAACUUAACAGUAAACCCGACAUUGUGUCGGGUUUACUUUAUGGCGCAAAAAGUGCGGUUAUUUUUCAAGGUGUUUUUUCGUGGGUGGUGUUCA .........((((((.....(((.((((((((((((.....))))))))))))))))))))).........(..(((..((((.((.......)).))))..)))..). (-31.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 406 79 36 163 123 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 389 48 2 80 30 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 387 58 12 89 45 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 379 49 14 162 127 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 377 49 1 154 97 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 371 50 12 128 89 aact:AA00401|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_slyD_(PPiase)_268361..268591_POSITIVE 363 53 15 139 93 aact:AA01553|5end_conserved_hypothetical_protein_1039220..1039450_POSITIVE 363 59 16 146 104 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 354 59 16 51 10 aact:AA01041|5end_hypothetical_protein_724853..725083_POSITIVE 351 69 23 216 175 aact:AA03000|5end_uracil-DNA_glycosylase_(UDG)_2095076..2095306_POSITIVE 345 80 31 162 97 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 337 52 11 142 103 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 335 69 21 89 47 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 334 80 36 192 151 aact:AA01192|5end_conserved_hypothetical_protein_(possible_membrane_protein)_817358..817588_POSITIVE 331 67 22 160 99 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 330 59 17 143 97 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 330 55 13 153 103 aact:AA01441|5end_tRNA/rRNA_methylase_966490..966720_POSITIVE 326 79 36 192 147 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 320 47 8 213 175 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 320 47 8 92 54 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 423 98 57 41 6 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 406 79 36 65 25 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 380 68 23 108 67 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 379 49 14 86 51 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 377 49 1 149 92 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 371 50 12 50 11 aact:AA00399|3end_conserved_hypothetical_protein_268363..268513_POSITIVE 370 67 21 54 6 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 363 53 15 139 93 aact:AA01552|3end_hypothetical_protein_1039300..1039450_POSITIVE 363 59 16 55 13 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 354 59 16 64 23 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 344 46 10 123 83 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE 341 62 21 48 7 aact:AA02540|3end_5-methyltetrahydropteroyltriglutamate-homocystein_e_methyltransferase_1769499..1769649_POSITIVE 335 69 21 108 66 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 330 55 13 81 31 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 326 79 36 112 67 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 320 47 8 89 51 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 318 45 17 76 49 aact:AA01046|3end_conserved_hypothetical_protein_729280..729430_POSITIVE 317 45 10 107 76 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 316 59 22 56 15 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 312 77 31 108 55 aact:AA03012|3end_conserved_hypothetical_protein_2104556..2104706_POSITIVE