Origin IGS: gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaattcgcaattatatttttcgtcgcttcttttagaataccaccattcgcaatccgcagtaggttgtatgtcgaataatcaatttgttagtcaatcaatcacatgaataaggagattatc Mask Tandem Repeat Region ================================================ gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Find is-nt database================================================ Query_seq: AA00381:AA00382|AA00381:AA00382:ornithine carbamoyltransferase:hypothetical protein:->->:258612..258732 121 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00381:AA00382|AA00381:AA00382:ornithine carbamoyltransferase:hypothetical protein:->->:258612..258732 121 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00381:AA00382|AA00381:AA00382:ornithine carbamoyltransferase:hypothetical protein:->->:258612..258732 121 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Predict ORF larger than 30AA ================================================ gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00381:AA00382|AA00381:AA00382:ornithine carbamoyltransferase:hypothetical protein:->->:258612..258732 121 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Intra-Species Hit: Count: 1 Min: 1 Max: 121 Len: 121 Subject: aact_AA00381_AA00382|ornithine carbamoyltransferase:hypothetical protein|POSITIVE:POSITIVE|[258612,258732]|121 HSP 1 e-value: 8.0E-63 bit: 240.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt gataatctccttattcatgtgattgattgactaacaaattgattattcgacatacaacctactgcggattgcgaatggtggtattctaaaagaagcgacgaaaaatataattgcgaatttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAAUCUCCUUAUUCAUGUGAUUGAUUGACUAACAAAUUGAUUAUUCGACAUACAACCUACUGCGGAUUGCGAAUGGUGGUAUUCUAAAAGAAGCGACGAAAAAUAUAAUUGCGAAUUUU .....(((((((......(((((..(((........)))..)))))..((.((((.(((...(((.....)))...))).))))))......))).)).)).................... (-15.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUUCGCAAUUAUAUUUUUCGUCGCUUCUUUUAGAAUACCACCAUUCGCAAUCCGCAGUAGGUUGUAUGUCGAAUAAUCAAUUUGUUAGUCAAUCAAUCACAUGAAUAAGGAGAUUAUC ..........................((((((...((((......))))..........((.(((((..((.(.(((((.....))))).).))..)))))))......))))))...... (-13.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 310 83 41 156 111 aact:AA01191|5end_AMP-binding_enzyme_816000..816230_POSITIVE 306 100 51 87 43 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 284 98 57 51 9 aact:AA00744|5end_aldose_1-epimerase_(mutarotase)_510138..510368_POSITIVE 282 92 51 58 26 aact:AA02554|5end_conserved_hypothetical_protein_1779735..1779965_POSITIVE 281 87 48 75 40 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 269 86 47 62 23 aact:AA00168|5end_possible_sigma_54_modulation_protein_119840..120070_POSITIVE 267 104 63 160 126 aact:AA01866|5end_thiamin_ABC_transporter,_permease_protein_1253313..1253543_POSITIVE 266 97 52 195 139 aact:AA01928|5end_ATP-dependent_RNA_helicase_1301986..1302216_POSITIVE 264 106 64 81 33 aact:AA02865|5end_iron-regulated_outer_membrane_protein_(omp64)_2010513..2010743_POSITIVE 264 97 55 61 23 aact:AA00303|5end_thymidylate_kinase_(DTMP)_206107..206337_POSITIVE 263 97 55 73 19 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 262 120 72 132 87 aact:AA02501|5end_DNA_polymerase_III,_subunits_gamma_and_tau_1735504..1735734_POSITIVE 261 97 56 68 25 aact:AA02436|5end_periplasmic_protein_1680497..1680727_POSITIVE 258 74 40 190 155 aact:AA01868|5end_biotin_synthetase_1255545..1255775_POSITIVE 258 100 58 89 45 aact:AA01162|5end_conserved_hypothetical_protein_(possible_transmembrane_protein)_795073..795303_POSITIVE 258 98 56 192 154 aact:AA00833|5end_UDP-N-acetylmuramate-alanine_ligase_573711..573941_POSITIVE 253 115 71 170 131 aact:AA01976|5end_colicin_V_production_protein_1336513..1336743_POSITIVE 253 78 44 124 92 aact:AA00110|5end_ATPase_involved_in_DNA_replication_79085..79315_POSITIVE 252 98 55 116 67 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 250 97 58 221 182 aact:AA02560|5end_integral_membrane_protein_1781412..1781642_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 81 41 151 108 aact:AA01190|3end_glycosyl_transferase_(possible_dolichol-phosphate_mannosyltransferase)_816077..816227_POSITIVE 267 104 63 76 42 aact:AA01865|3end_thiamin_ABC_transporter,_periplasmic-binding_protein_1253309..1253459_POSITIVE 266 97 52 120 64 aact:AA01927|3end_conserved_hypothetical_protein_1301991..1302141_POSITIVE 264 97 55 79 41 aact:AA00302|3end_DNA_polymerase_III,_delta'_subunit_206205..206355_POSITIVE 262 120 72 126 81 aact:AA02500|3end_conserved_hypothetical_protein_1735578..1735728_POSITIVE 258 74 40 142 107 aact:AA01867|3end_thiamine_transport_ATP-binding_protein_1255577..1255727_POSITIVE 253 78 44 112 80 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 250 77 41 133 86 aact:AA02315|3end_conserved_hypothetical_protein_1581752..1581902_POSITIVE 249 103 61 90 45 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE 249 83 46 71 37 aact:AA00164|3end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_117445..117595_POSITIVE 247 100 59 73 33 aact:AA00989|3end_hypothetical_protein_692644..692794_POSITIVE 246 103 64 99 50 aact:AA02263|3end_conserved_hypothetical_protein_1539765..1539915_POSITIVE 241 81 55 130 99 aact:AA00887|3end_hypothetical_protein_616956..617106_POSITIVE 240 101 68 54 13 aact:AA02218|3end_deoxyribose-phosphate_aldolase_(fragment?)_1510086..1510236_POSITIVE 240 100 51 70 24 aact:AA01696|3end_conserved_hypothetical_protein_1153730..1153880_POSITIVE 235 108 62 92 35 aact:AA01177|3end_beta-ketoacyl-ACP_synthase_IV_808053..808203_POSITIVE 233 101 62 40 2 aact:AA00965|3end_hypothetical_protein_674600..674750_POSITIVE 231 100 58 116 70 aact:AA02335|3end_6-O-methylguanine-DNA_methyltransferase_1597430..1597580_POSITIVE 231 100 59 104 68 aact:AA01335|3end_primosomal_protein_N'_904109..904259_POSITIVE 231 85 42 149 88 aact:AA01024|3end_thiamine_biosynthesis_lipoprotein_precursor_717602..717752_POSITIVE