Origin IGS: taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgagtgggcatgattcaaattaacatgcttgatttaatttgaggaaacaaaagtaatcaaacggcaaatttacttctgtttttgatagcacaaaatggctgatggttacccaccagccatttagcctgagaaacaggacgaaatcgaatcaaaatta Mask Tandem Repeat Region ================================================ taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Find is-nt database================================================ Query_seq: AA00351:AA00354|AA00351:AA00354:50S ribosomal protein L7/L12:hypothetical protein:->->:234673..234829 157 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00351:AA00354|AA00351:AA00354:50S ribosomal protein L7/L12:hypothetical protein:->->:234673..234829 157 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00351:AA00354|AA00351:AA00354:50S ribosomal protein L7/L12:hypothetical protein:->->:234673..234829 157 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 3 End: 98 .. M L I R F R P V S Q A K W L V G N H Q P F C A I K N R S K F A V ........................................................... Protein_Len: 38 Strand: + Start: 22 End: 135 ..................... M F L R L N G W W V T I S H F V L S K T E V N L P F D Y F C F L K L N Q A C ...................... taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 95 HP_score: -14.7 Tail_Score: -2.62184 Start: 34 End: 67 Full_Region: GTCCTGTTTCTCAGG CTAAATGGCTGGTGG GTAA CCATCAGCCATTTTG TGCTATCAAAAACAG .................................CTAAATGGCTGGTGGGTAACCATCAGCCATTTTG.......................................................................................... TransTerm Strand: + Conf: 100 HP_score: -13.2 Tail_Score: -4.50976 Start: 39 End: 62 Full_Region: GTTTCTCAGGCTAAA TGGCTGGTGG GTAA CCATCAGCCA TTTTGTGCTATCAAA ......................................TGGCTGGTGGGTAACCATCAGCCA............................................................................................... TransTerm Strand: - Conf: 84 HP_score: -11.0 Tail_Score: -3.59979 Start: 39 End: 62 Full_Region: tttgatagcacaaaa tggctgatgg ttac ccaccagcca tttagcctgagaaac ......................................tggctgatggttacccaccagcca............................................................................................... Find igs database================================================ Query_seq: AA00351:AA00354|AA00351:AA00354:50S ribosomal protein L7/L12:hypothetical protein:->->:234673..234829 157 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Intra-Species Hit: Count: 1 Min: 1 Max: 157 Len: 157 Subject: aact_AA00351_AA00354|50S ribosomal protein L7/L12:hypothetical protein|POSITIVE:POSITIVE|[234673,234829]|157 HSP 1 e-value: 4.0E-84 bit: 311.0 Len: 157 Query Start:1 Query End:157 Subject Strand: POSITIVE Subject Start: 1 Subject End: 157 taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca taattttgattcgatttcgtcctgtttctcaggctaaatggctggtgggtaaccatcagccattttgtgctatcaaaaacagaagtaaatttgccgtttgattacttttgtttcctcaaattaaatcaagcatgttaatttgaatcatgcccactca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUGAUUCGAUUUCGUCCUGUUUCUCAGGCUAAAUGGCUGGUGGGUAACCAUCAGCCAUUUUGUGCUAUCAAAAACAGAAGUAAAUUUGCCGUUUGAUUACUUUUGUUUCCUCAAAUUAAAUCAAGCAUGUUAAUUUGAAUCAUGCCCACUCA ......((((((((.......((((((....(((.(((((((((((((....)))))))))))))...))).....)))))).............((((((((...((((......))))...))))))))........)))))))).......... (-47.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGUGGGCAUGAUUCAAAUUAACAUGCUUGAUUUAAUUUGAGGAAACAAAAGUAAUCAAACGGCAAAUUUACUUCUGUUUUUGAUAGCACAAAAUGGCUGAUGGUUACCCACCAGCCAUUUAGCCUGAGAAACAGGACGAAAUCGAAUCAAAAUUA ..........((((((.........(((((((((..((((..(....)..)))))))))...)))).......((((((((((.(..((...(((((((((.(((....))).))))))))).)).).)))))))))).......))))))...... (-41.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 400 49 1 147 92 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 368 69 21 78 19 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 344 70 23 63 8 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 333 70 22 139 77 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 323 60 21 185 134 aact:AA02634|5end_rhamnosyltransferase;_glycosyltransferase_homolog_2_1832816..1833046_POSITIVE 316 70 21 215 154 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 314 75 31 190 153 aact:AA01259|5end_protoporphyrinogen_oxidase_854354..854584_POSITIVE 306 69 21 177 138 aact:AA01295|5end_3-dehydroquinate_synthase_877272..877502_POSITIVE 305 70 21 67 18 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 300 56 13 91 25 aact:AA00614|5end_ribonuclease_HII_(RNase_HII)_419890..420120_POSITIVE 299 58 12 175 133 aact:AA01792|5end_conserved_hypothetical_protein_1208058..1208288_POSITIVE 297 61 41 26 8 aact:AA02600|5end_conserved_hypothetical_protein_1810672..1810902_POSITIVE 292 69 21 47 7 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 292 76 42 208 165 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 290 70 21 73 7 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 290 61 22 143 96 aact:AA00709|5end_metallocluster_assembly_(NifU_protein)_487378..487608_POSITIVE 289 60 11 61 6 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 288 60 18 161 109 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 284 80 32 203 148 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 283 70 25 180 141 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 416 65 22 55 9 aact:AA00351|3end_50S_ribosomal_protein_L7/L12_234612..234762_POSITIVE 416 65 22 111 65 aact:AA00353|3end_hypothetical_protein_234668..234818_POSITIVE 387 47 1 148 99 aact:AA00236|3end_anaerobic_ribonucleoside-triphosphate_reductase_171021..171171_POSITIVE 345 60 11 118 50 aact:AA02601|3end_ATP-dependent_helicase_1813309..1813459_POSITIVE 306 60 19 89 30 aact:AA02421|3end_conserved_hypothetical_protein_1667300..1667450_POSITIVE 302 60 18 149 98 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 300 56 13 95 29 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 296 60 11 151 96 aact:AA01757|3end_cobalt_transport_ATP-binding_protein_1187837..1187987_POSITIVE 290 61 22 80 33 aact:AA00708|3end_cysteine_desulfurase_487395..487545_POSITIVE 289 60 11 56 1 aact:AA02156|3end_conserved_hypothetical_protein_1475326..1475476_POSITIVE 288 59 12 91 48 aact:AA00741|3end_possible_2-oxoglutarate/malate_transporter_508076..508226_POSITIVE 282 74 38 68 26 aact:AA02880|3end_50S_ribosomal_protein_L21_2021145..2021295_POSITIVE 282 59 13 141 94 aact:AA01948|3end_ABC_transporter_permease,_membrane_spanning_protein_1318196..1318346_POSITIVE 277 61 29 34 7 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 276 74 31 115 80 aact:AA00521|3end_phosphatase_358324..358474_POSITIVE 273 49 12 53 13 aact:AA00580|3end_conserved_hypothetical_protein_(possible_membrane_protein)_397628..397778_POSITIVE 272 73 36 61 22 aact:AA00914|3end_aminoacyl-histidine_dipeptidase;_Beta-alanyl-histidine_dipeptidase_629575..629725_POSITIVE 271 70 32 38 6 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 269 80 42 60 19 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 269 76 39 150 107 aact:AA00650|3end_15_kDa_peptidoglycan-associated_protein_445030..445180_POSITIVE