Origin IGS: taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaaatccttgaatgtcggttaaatcggataagttaacgcaaacgtggcagccattatactgcttatgtccggtttcgcaataaatagcgcagaatagtta Mask Tandem Repeat Region ================================================ taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Find is-nt database================================================ Query_seq: AA00329:AA00330|AA00329:AA00330:molybdopterin-guanine dinucleotide biosynthesis protein:conserved hypothetical protein:->->:223408..223508 101 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00329:AA00330|AA00329:AA00330:molybdopterin-guanine dinucleotide biosynthesis protein:conserved hypothetical protein:->->:223408..223508 101 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00329:AA00330|AA00329:AA00330:molybdopterin-guanine dinucleotide biosynthesis protein:conserved hypothetical protein:->->:223408..223508 101 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Predict ORF larger than 30AA ================================================ taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00329:AA00330|AA00329:AA00330:molybdopterin-guanine dinucleotide biosynthesis protein:conserved hypothetical protein:->->:223408..223508 101 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA00329_AA00330|molybdopterin-guanine dinucleotide biosynthesis protein:conserved hypothetical protein|POSITIVE:POSITIVE|[223408,223508]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt taactattctgcgctatttattgcgaaaccggacataagcagtataatggctgccacgtttgcgttaacttatccgatttaaccgacattcaaggattttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUAUUCUGCGCUAUUUAUUGCGAAACCGGACAUAAGCAGUAUAAUGGCUGCCACGUUUGCGUUAACUUAUCCGAUUUAACCGACAUUCAAGGAUUUUU .....(((((.(((........)))....((((.....(((((......))))).(((....))).......))))................))))).... (-17.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUCCUUGAAUGUCGGUUAAAUCGGAUAAGUUAACGCAAACGUGGCAGCCAUUAUACUGCUUAUGUCCGGUUUCGCAAUAAAUAGCGCAGAAUAGUUA ..................((.((((((((((.(((......)))..(((((........)))))..)))))))))).))......((((........)))) (-19.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 58 11 60 4 aact:AA02111|5end_killing_protein_suppressor_1436877..1437107_POSITIVE 326 55 11 163 117 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 322 88 41 102 55 aact:AA02811|5end_hypothetical_protein_1979582..1979812_POSITIVE 319 64 23 165 118 aact:AA01867|5end_thiamine_transport_ATP-binding_protein_1254853..1255083_POSITIVE 317 41 3 72 29 aact:AA01866|5end_thiamin_ABC_transporter,_permease_protein_1253313..1253543_POSITIVE 317 68 21 102 50 aact:AA00734|5end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502528..502758_POSITIVE 314 60 11 177 121 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 309 85 47 156 116 aact:AA02570|5end_conserved_hypothetical_protein_1789150..1789380_POSITIVE 302 66 21 72 19 aact:AA01815|5end_probable_sodium-dependent_transmembrane_transport_protein_1222541..1222771_POSITIVE 299 56 11 219 162 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 289 63 21 64 9 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 284 58 11 216 166 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 283 58 11 205 156 aact:AA01184|5end_hypothetical_protein_810838..811068_POSITIVE 283 60 13 69 21 aact:AA00345|5end_transcription_antitermination_protein_231430..231660_POSITIVE 280 70 24 165 115 aact:AA01629|5end_phosphatidylglycerophosphatase_A_1101881..1102111_POSITIVE 279 60 13 48 3 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 279 70 23 65 17 aact:AA00292|5end_dihydrofolate_reductase_201800..202030_POSITIVE 276 65 21 202 165 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 275 88 46 56 16 aact:AA00362|5end_30S_ribosomal_protein_S3_244847..245077_POSITIVE 274 70 23 200 158 aact:AA01840|5end_conserved_hypothetical_protein_1237088..1237318_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 55 11 128 82 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 309 85 47 110 70 aact:AA02569|3end_hypothetical_protein_1789184..1789334_POSITIVE 289 68 22 130 92 aact:AA01871|3end_conserved_hypothetical_protein_1258710..1258860_POSITIVE 283 60 13 64 16 aact:AA00344|3end_preprotein_translocase_secE_subunit_231505..231655_POSITIVE 279 70 23 90 42 aact:AA00291|3end_hypothetical_protein_201905..202055_POSITIVE 273 59 11 67 5 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 269 63 24 117 80 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 269 59 11 65 27 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 269 88 51 149 111 aact:AA02313|3end_conserved_hypothetical_protein_1580526..1580676_POSITIVE 267 99 52 96 49 aact:AA01579|3end_transcriptional_regulatory_protein_1065283..1065433_POSITIVE 264 59 12 72 32 aact:AA02527|3end_integration_host_factor,beta-subunit_1755984..1756134_POSITIVE 264 67 24 99 47 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 263 77 32 150 100 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 261 69 24 151 102 aact:AA01628|3end_thiamin-monophosphate_kinase_1101948..1102098_POSITIVE 260 65 21 110 66 aact:AA00715|3end_[2Fe-2S]_ferredoxin_491050..491200_POSITIVE 259 44 9 28 4 aact:AA00361|3end_50S_ribosomal_protein_L16_244910..245060_POSITIVE 259 67 21 83 41 aact:AA00160|3end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_113434..113584_POSITIVE 258 66 23 86 29 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 257 88 41 81 33 aact:AA01117|3end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_769375..769525_POSITIVE 256 84 49 81 50 aact:AA02778|3end_hypothetical_protein_1942802..1942952_POSITIVE