Origin IGS: acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 acgcgtcgcccgcccgccggctaaaattaccgcgctgattgaaattgtcatgttttcttccgtcgttatattttcttccggggttaattcaagtatgatcccgccgtttatttctcaataataaggaaaagcgt Mask Tandem Repeat Region ================================================ acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Find is-nt database================================================ Query_seq: AA00327:AA00329|AA00327:AA00329:conserved hypothetical protein:molybdopterin-guanine dinucleotide biosynthesis protein:->->:222743..222876 134 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00327:AA00329|AA00327:AA00329:conserved hypothetical protein:molybdopterin-guanine dinucleotide biosynthesis protein:->->:222743..222876 134 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00327:AA00329|AA00327:AA00329:conserved hypothetical protein:molybdopterin-guanine dinucleotide biosynthesis protein:->->:222743..222876 134 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 24 End: 134 ....................... M N G G I I L E L T P E E N I T T E E N M T I S I S A V I L A G G R A T R Protein_Len: 31 Strand: - Start: 17 End: 109 ................ Q S I F P P I M S S N V G S S F I V V S S F M V I E I L A T M ......................... acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00327:AA00329|AA00327:AA00329:conserved hypothetical protein:molybdopterin-guanine dinucleotide biosynthesis protein:->->:222743..222876 134 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Intra-Species Hit: Count: 1 Min: 1 Max: 134 Len: 134 Subject: aact_AA00327_AA00329|conserved hypothetical protein:molybdopterin-guanine dinucleotide biosynthesis protein|POSITIVE:POSITIVE|[222743,222876]|134 HSP 1 e-value: 2.0E-70 bit: 266.0 Len: 134 Query Start:1 Query End:134 Subject Strand: POSITIVE Subject Start: 1 Subject End: 134 acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt acgcttttccttattattgagaaataaacggcgggatcatacttgaattaaccccggaagaaaatataacgacggaagaaaacatgacaatttcaatcagcgcggtaattttagccggcgggcgggcgacgcgt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGCUUUUCCUUAUUAUUGAGAAAUAAACGGCGGGAUCAUACUUGAAUUAACCCCGGAAGAAAAUAUAACGACGGAAGAAAACAUGACAAUUUCAAUCAGCGCGGUAAUUUUAGCCGGCGGGCGGGCGACGCGU .((((..(((.....(((((((......(((.((..(((....))).....)))))................(....)............)))))))..((.((((.......)))))))))..))))...... (-28.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGCGUCGCCCGCCCGCCGGCUAAAAUUACCGCGCUGAUUGAAAUUGUCAUGUUUUCUUCCGUCGUUAUAUUUUCUUCCGGGGUUAAUUCAAGUAUGAUCCCGCCGUUUAUUUCUCAAUAAUAAGGAAAAGCGU ((((...(((.(....).)))..........((((.((..(((((......)))))..)).).)))....(((((((.((((((((.........))))))))....(((((....))))).))))))).)))) (-24.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 400 129 86 148 87 aact:AA02990|5end_beta_hexosaminadase_2089473..2089703_POSITIVE 377 128 91 125 68 aact:AA01108|5end_hypothetical_protein_763646..763876_POSITIVE 372 127 95 54 23 aact:AA02604|5end_thioredoxin_reductase_1815552..1815782_POSITIVE 348 128 83 99 52 aact:AA01428|5end_acetolactate_synthase_958688..958918_POSITIVE 343 128 81 75 18 aact:AA01449|5end_prophage_CP4-57_integrase_970569..970799_POSITIVE 338 128 91 148 113 aact:AA01119|5end_hypothetical_protein_769291..769521_POSITIVE 337 126 95 42 17 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 333 130 88 225 189 aact:AA01563|5end_transketolase_isozyme_1_1046969..1047199_POSITIVE 333 128 96 170 127 aact:AA01018|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_711663..711893_POSITIVE 332 126 81 60 3 aact:AA01523|5end_conserved_hypothetical_protein_1023205..1023435_POSITIVE 331 128 88 114 67 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 330 130 83 63 23 aact:AA00689|5end_GTP-binding_protein_470433..470663_POSITIVE 329 128 100 70 35 aact:AA01614|5end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1089698..1089928_POSITIVE 329 127 100 54 19 aact:AA01413|5end_conserved_hypothetical_protein_951773..952003_POSITIVE 328 128 99 187 143 aact:AA01917|5end_hypothetical_protein_1290716..1290946_POSITIVE 327 128 97 66 27 aact:AA01857|5end_glutamate-ammonia-ligase_adenylyltransferase_1247347..1247577_POSITIVE 324 128 98 58 26 aact:AA01662|5end_molybdopterin_biosynthesis_protein_1126131..1126361_POSITIVE 322 128 89 169 128 aact:AA02422|5end_conserved_hypothetical_protein_1668014..1668244_POSITIVE 322 128 89 169 128 aact:AA02298|5end_conserved_hypothetical_protein_1570588..1570818_POSITIVE 322 128 89 169 128 aact:AA00622|5end_conserved_hypothetical_protein_429692..429922_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 400 129 86 143 82 aact:AA02989|3end_conserved_hypothetical_protein_2089548..2089698_POSITIVE 365 125 91 137 96 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE 353 128 87 150 105 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 333 130 88 147 111 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 331 128 88 143 96 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 328 128 99 144 100 aact:AA01915|3end_possible_negative_regulator_of_translation_1290753..1290903_POSITIVE 327 127 82 137 98 aact:AA00884|3end_sodium-dependent_transporter_615971..616121_POSITIVE 324 128 98 39 7 aact:AA01661|3end_molybdopterin_biosynthesis_protein_1126192..1126342_POSITIVE 320 129 96 128 94 aact:AA00078|3end_hypothetical_protein_57691..57841_POSITIVE 319 129 87 136 98 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 318 130 97 149 101 aact:AA00380|3end_carbamate_kinase_257537..257687_POSITIVE 317 127 99 105 74 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 317 127 96 67 26 aact:AA00302|3end_DNA_polymerase_III,_delta'_subunit_206205..206355_POSITIVE 316 129 83 137 81 aact:AA00163|3end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116772..116922_POSITIVE 313 129 99 72 26 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 312 129 91 143 110 aact:AA00723|3end_peptidase_B_(leucyl_aminopeptidase)_497841..497991_POSITIVE 309 127 93 124 88 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 304 129 83 146 84 aact:AA02439|3end_integral_membrane_protein;_possible_ABC_transporter_permease_1685118..1685268_POSITIVE 304 127 91 151 117 aact:AA01117|3end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_769375..769525_POSITIVE 302 128 83 148 83 aact:AA02405|3end_conserved_hypothetical_protein_1652740..1652890_POSITIVE