Origin IGS: attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggtatttttattgatatataggcgtttcctaaccattccgttgctatgttaaggtaagccgatttaacaaaatcggagattgattttttctgaataaagcctctaaaacaat Mask Tandem Repeat Region ================================================ attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Find is-nt database================================================ Query_seq: AA00321:AA00323|AA00321:AA00323:hypothetical protein:conserved hypothetical protein (possible SAM-dependent methyltransferase):->->:219413..219526 114 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00321:AA00323|AA00321:AA00323:hypothetical protein:conserved hypothetical protein (possible SAM-dependent methyltransferase):->->:219413..219526 114 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00321:AA00323|AA00321:AA00323:hypothetical protein:conserved hypothetical protein (possible SAM-dependent methyltransferase):->->:219413..219526 114 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 18 End: 113 ................. M Q K K S I S D F V K S A Y L N I A T E W L G N A Y I S I K I P . attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00321:AA00323|AA00321:AA00323:hypothetical protein:conserved hypothetical protein (possible SAM-dependent methyltransferase):->->:219413..219526 114 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA00321_AA00323|hypothetical protein:conserved hypothetical protein (possible SAM-dependent methyltransferase)|POSITIVE:POSITIVE|[219413,219526]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta attgttttagaggctttattcagaaaaaatcaatctccgattttgttaaatcggcttaccttaacatagcaacggaatggttaggaaacgcctatatatcaataaaaataccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGUUUUAGAGGCUUUAUUCAGAAAAAAUCAAUCUCCGAUUUUGUUAAAUCGGCUUACCUUAACAUAGCAACGGAAUGGUUAGGAAACGCCUAUAUAUCAAUAAAAAUACCUA ((((......((((......................(((((((....))))))).....((((((....(....)....))))))....))))......))))........... (-19.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUAUUUUUAUUGAUAUAUAGGCGUUUCCUAACCAUUCCGUUGCUAUGUUAAGGUAAGCCGAUUUAACAAAAUCGGAGAUUGAUUUUUUCUGAAUAAAGCCUCUAAAACAAU .((((.......(((.((....(((....(((...(((.........)))...)))...)))....)).)))..(((((((.......))))))).....)))).......... (-18.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 303 80 33 63 8 aact:AA01508|5end_anaerobic_C4-dicarboxylate_transporter_1013198..1013428_POSITIVE 275 98 51 194 136 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 274 92 51 71 25 aact:AA02548|5end_desulfoviridin_gamma_subunit_1775377..1775607_POSITIVE 265 80 32 184 126 aact:AA00682|5end_S-adenosylmethionine-dependent_methyltransferase_466322..466552_POSITIVE 260 91 52 52 4 aact:AA00071|5end_conserved_hypothetical_protein_(possible_zinc_protease)_53463..53693_POSITIVE 253 91 54 58 11 aact:AA02313|5end_conserved_hypothetical_protein_1580156..1580386_POSITIVE 252 91 51 192 141 aact:AA02021|5end_anaerobic_glycerol-3-phosphate_dehydrogenase_1374935..1375165_POSITIVE 250 86 51 219 178 aact:AA02391|5end_hypothetical_protein_1640525..1640755_POSITIVE 243 60 12 227 175 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 242 90 52 100 67 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 242 91 53 183 145 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 234 100 51 218 176 aact:AA01138|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_780991..781221_POSITIVE 233 92 52 197 157 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 233 89 50 199 162 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 232 79 35 145 101 aact:AA01240|5end_30S_ribosomal_protein_S11_844875..845105_POSITIVE 230 88 51 58 10 aact:AA02579|5end_aminotransferase_nifS_protein_1795773..1796003_POSITIVE 229 57 11 141 94 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 228 90 52 82 46 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 228 55 13 140 98 aact:AA00614|5end_ribonuclease_HII_(RNase_HII)_419890..420120_POSITIVE 227 80 33 68 20 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 274 92 51 48 2 aact:AA02547|3end_transmembrane_protein_1775434..1775584_POSITIVE 253 91 54 62 15 aact:AA02312|3end_conserved_hypothetical_protein_1580240..1580390_POSITIVE 242 90 52 53 20 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 240 88 45 76 39 aact:AA01393|3end_ABC_transporter,_ATP-binding_protein_942029..942179_POSITIVE 239 102 71 75 33 aact:AA01173|3end_VapD-homolog_804385..804535_POSITIVE 232 79 35 126 82 aact:AA01239|3end_30S_ribosomal_protein_S13_844936..845086_POSITIVE 230 88 51 49 1 aact:AA02577|3end_nuclease_1795844..1795994_POSITIVE 228 55 13 144 102 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 225 87 48 81 39 aact:AA01276|3end_heptosyltransferase_III_865844..865994_POSITIVE 225 75 35 55 12 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 224 94 51 131 95 aact:AA00015|3end_malate_dehydrogenase_8333..8483_POSITIVE 220 91 53 57 19 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 220 91 51 149 111 aact:AA01510|3end_ABC_transporter,_ATP-binding_protein/permease_1018165..1018315_POSITIVE 218 92 51 118 77 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 216 60 11 147 101 aact:AA02134|3end_aminopeptidase_N_1458867..1459017_POSITIVE 215 90 41 105 59 aact:AA01291|3end_excinuclease_ABC_subunit_A_876001..876151_POSITIVE 214 59 32 72 37 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 212 80 33 124 90 aact:AA01810|3end_electron_transport_complex_protein_1220406..1220556_POSITIVE 211 60 15 92 53 aact:AA02190|3end_hydrogenase-2_component_protein_1493271..1493421_POSITIVE 211 92 51 147 109 aact:AA01204|3end_hypothetical_protein_825196..825346_POSITIVE