Origin IGS: taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagatgtcctgtgtgcagtaagtaatgtaaaacggaacgatcgccgtagtatagagcgaagcatgaaagaaacgcaaggcgagcagggaatattatctcaattattccgtcacaatgaaaaaattcgataccgaacctgaaccaagtatcgggatacagagacccggcatagcctgcttaatttatctttatttttta Mask Tandem Repeat Region ================================================ taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Find is-nt database================================================ Query_seq: AA00314:AA00316|AA00314:AA00316:conserved hypothetical protein (possible Kmt1 protein):conserved hypothetical protein:->->:214387..214584 198 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00314:AA00316|AA00314:AA00316:conserved hypothetical protein (possible Kmt1 protein):conserved hypothetical protein:->->:214387..214584 198 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00314:AA00316|AA00314:AA00316:conserved hypothetical protein (possible Kmt1 protein):conserved hypothetical protein:->->:214387..214584 198 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 108 End: 197 ........................................................................................................... M P C S P C V S F M L R S I L R R S F R F T L L T A H R T S . Protein_Len: 53 Strand: + Start: 40 End: 198 ....................................... M Y P D T W F R F G I E F F H C D G I I E I I F P A R L A F L S C F A L Y Y G D R S V L H Y L L H T G H L Protein_Len: 43 Strand: - Start: 62 End: 190 ............................................................. T R Y R I K E N H R F L Q S L I G Q E G Q T E K M S R E I S R R D N R K V N S V A C M ........ taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 80 HP_score: -4.4 Tail_Score: -5.32944 Start: 64 End: 75 Full_Region: GATACTTGGTTCAGG TTCG GTAT CGAA TTTTTTCATTGTGAC ...............................................................TTCGGTATCGAA........................................................................................................................... Find igs database================================================ Query_seq: AA00314:AA00316|AA00314:AA00316:conserved hypothetical protein (possible Kmt1 protein):conserved hypothetical protein:->->:214387..214584 198 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Intra-Species Hit: Count: 1 Min: 1 Max: 198 Len: 198 Subject: aact_AA00314_AA00316|conserved hypothetical protein (possible Kmt1 protein):conserved hypothetical protein|POSITIVE:POSITIVE|[214387,214584]|198 HSP 1 e-value: 1.0E-108 bit: 392.0 Len: 198 Query Start:1 Query End:198 Subject Strand: POSITIVE Subject Start: 1 Subject End: 198 taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt taaaaaataaagataaattaagcaggctatgccgggtctctgtatcccgatacttggttcaggttcggtatcgaattttttcattgtgacggaataattgagataatattccctgctcgccttgcgtttctttcatgcttcgctctatactacggcgatcgttccgttttacattacttactgcacacaggacatctt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAUAAAGAUAAAUUAAGCAGGCUAUGCCGGGUCUCUGUAUCCCGAUACUUGGUUCAGGUUCGGUAUCGAAUUUUUUCAUUGUGACGGAAUAAUUGAGAUAAUAUUCCCUGCUCGCCUUGCGUUUCUUUCAUGCUUCGCUCUAUACUACGGCGAUCGUUCCGUUUUACAUUACUUACUGCACACAGGACAUCUU .........(((((.......((((((...((.(((((((..(((.(((.(((.(((...(((((((....)))))))..)))..))).))).)))...)))).......))).))..))).)))...((((...(((.(((((.........)))))..((........))..........)))...)))).))))) (-41.91) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAUGUCCUGUGUGCAGUAAGUAAUGUAAAACGGAACGAUCGCCGUAGUAUAGAGCGAAGCAUGAAAGAAACGCAAGGCGAGCAGGGAAUAUUAUCUCAAUUAUUCCGUCACAAUGAAAAAAUUCGAUACCGAACCUGAACCAAGUAUCGGGAUACAGAGACCCGGCAUAGCCUGCUUAAUUUAUCUUUAUUUUUUA ((((((..((..((((.....((..((((..((((........))))..))))..))...))))...))....(((.(((..((.(((.......((((...(((((((..((..........((((....))))..........))..)))))))..))))))).))...))))))......))))))......... (-44.06) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 391 59 18 96 55 aact:AA01873|5end_mannose-6-phosphate_isomerase_1260658..1260888_POSITIVE 381 70 25 104 42 aact:AA01892|5end_hypothetical_protein_1276688..1276918_POSITIVE 360 158 112 154 120 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 344 170 123 228 180 aact:AA01490|5end_ATP_synthase_F0,_subunit_a_1001504..1001734_POSITIVE 344 160 118 144 82 aact:AA01242|5end_DNA-directed_RNA_polymerase_alpha_subunit_845943..846173_POSITIVE 342 68 21 73 24 aact:AA02763|5end_DNA_polymerase_III,_epsilon_subunit_1933696..1933926_POSITIVE 342 70 22 228 167 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 342 70 22 108 47 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 339 171 135 150 116 aact:AA00056|5end_cytochrome_c-type_biogenesis_protein_43925..44155_POSITIVE 334 71 33 38 2 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 332 157 114 210 173 aact:AA02368|5end_conserved_hypothetical_protein_1623544..1623774_POSITIVE 332 70 22 79 29 aact:AA01776|5end_conserved_hypothetical_protein_1196074..1196304_POSITIVE 328 62 21 230 186 aact:AA02678|5end_formate_dehydrogenase,_gamma_subunit_1865021..1865251_POSITIVE 325 157 112 80 27 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 323 160 112 59 7 aact:AA01465|5end_mannose-specific_phosphotransferase_element_984472..984702_POSITIVE 322 188 141 166 125 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 322 70 21 61 15 aact:AA01983|5end_maltose_regulon_positive_regulatory_protein_1341947..1342177_POSITIVE 321 159 112 207 141 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 320 160 111 125 54 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 319 67 22 188 134 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 70 25 120 58 aact:AA01891|3end_pyridoxine_kinase_1276784..1276934_POSITIVE 360 158 112 149 115 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 344 160 118 112 50 aact:AA01241|3end_30S_ribosomal_protein_S4_845991..846141_POSITIVE 339 171 135 147 113 aact:AA00055|3end_cytochrome_c-type_biogenesis_protein_44002..44152_POSITIVE 332 70 22 107 57 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 329 175 132 80 37 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 325 158 113 134 80 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 325 158 113 62 8 aact:AA00533|3end_conserved_hypothetical_protein_365522..365672_POSITIVE 322 67 22 138 81 aact:AA00116|3end_conserved_hypothetical_protein_88207..88357_POSITIVE 319 157 116 133 97 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 317 130 81 124 86 aact:AA02708|3end_probable_glyoxalase_II_family_protein_1890641..1890791_POSITIVE 313 155 111 67 33 aact:AA01962|3end_recombination_protein_rec2_1328706..1328856_POSITIVE 311 70 30 76 31 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 311 160 113 60 2 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 309 156 113 140 93 aact:AA01067|3end_30S_ribosomal_protein_S2_739697..739847_POSITIVE 309 144 107 127 89 aact:AA00804|3end_fumarate_reductase_flavoprotein_subunit_A_551655..551805_POSITIVE 305 170 123 85 34 aact:AA02763|3end_DNA_polymerase_III,_epsilon_subunit_1934477..1934627_POSITIVE 298 177 140 144 110 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 298 160 111 140 71 aact:AA00987|3end_tetracenomycin_polyketide_synthesis_O-methyltransferase_690586..690736_POSITIVE 296 160 111 132 86 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE