Origin IGS: aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtatttctccaaataataaagtttgttgatctaacggtatccattctgccggatcttaaaataaaaaaacgtgagcaatgtcacaaaatttaacattattatttatttttgttattttatgtgaaaaatgaatggaataacaacaaaataaacaaaaagagatataatgacgaaaattttttggaggggtt Mask Tandem Repeat Region ================================================ aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Find is-nt database================================================ Query_seq: AA00308:AA00309|AA00308:AA00309:glycerol-3-phosphate regulon repressor:3-hydroxyisobutyrate dehydrogenase; 2-hydroxy-3-oxopropionate reductase:->->:209233..209424 192 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00308:AA00309|AA00308:AA00309:glycerol-3-phosphate regulon repressor:3-hydroxyisobutyrate dehydrogenase; 2-hydroxy-3-oxopropionate reductase:->->:209233..209424 192 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00308:AA00309|AA00308:AA00309:glycerol-3-phosphate regulon repressor:3-hydroxyisobutyrate dehydrogenase; 2-hydroxy-3-oxopropionate reductase:->->:209233..209424 192 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 29 End: 133 ............................ M S F C L F C C Y S I H F S H K I T K I N N N V K F C D I A H V F L F ........................................................... aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 74 End: 103 .........................................................................TTTAACATTATTATTTATTTTTGTTATTTT......................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00308:AA00309|AA00308:AA00309:glycerol-3-phosphate regulon repressor:3-hydroxyisobutyrate dehydrogenase; 2-hydroxy-3-oxopropionate reductase:->->:209233..209424 192 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcacataaaataacaaaaataaataataatgttaaattttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Intra-Species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: aact_AA00308_AA00309|glycerol-3-phosphate regulon repressor:3-hydroxyisobutyrate dehydrogenase; 2-hydroxy-3-oxopropionate reductase|POSITIVE:POSITIVE|[209233,209424]|192 HSP 1 e-value: 1.0E-32 bit: 141.0 Len: 71 Query Start:1 Query End:71 Subject Strand: POSITIVE Subject Start: 1 Subject End: 71 aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcac......................................................................................................................... aacccctccaaaaaattttcgtcattatatctctttttgtttattttgttgttattccattcatttttcac......................................................................................................................... HSP 2 e-value: 2.0E-30 bit: 133.0 Len: 88 Query Start:105 Query End:192 Subject Strand: POSITIVE Subject Start: 105 Subject End: 192 ........................................................................................................tttgtgacattgctcacgnnnnnnnattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc ........................................................................................................tttgtgacattgctcacgtttttttattttaagatccggcagaatggataccgttagatcaacaaactttattatttggagaaatacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCCCUCCAAAAAAUUUUCGUCAUUAUAUCUCUUUUUGUUUAUUUUGUUGUUAUUCCAUUCAUUUUUCACAUAAAAUAACAAAAAUAAAUAAUAAUGUUAAAUUUUGUGACAUUGCUCACGUUUUUUUAUUUUAAGAUCCGGCAGAAUGGAUACCGUUAGAUCAACAAACUUUAUUAUUUGGAGAAAUACC .....(((((((.(((...................((((((.((((....((...(((((((.....(((((.....(((((..............))))).....)))))...((((...(((((........)))))..))))))))))).))....)))).))))))....))).)))))))....... (-29.84) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUAUUUCUCCAAAUAAUAAAGUUUGUUGAUCUAACGGUAUCCAUUCUGCCGGAUCUUAAAAUAAAAAAACGUGAGCAAUGUCACAAAAUUUAACAUUAUUAUUUAUUUUUGUUAUUUUAUGUGAAAAAUGAAUGGAAUAACAACAAAAUAAACAAAAAGAGAUAUAAUGACGAAAAUUUUUUGGAGGGGUU ...((..(((((((.(((....((((((.......(((((.......))))).(((((.............((((......)))).................(((((((((((((((((((...........)))))))))))..))))))))......))))).....)))))).))).)))))))..)). (-38.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 183 141 157 114 aact:AA01579|5end_transcriptional_regulatory_protein_1064247..1064477_POSITIVE 271 167 121 190 137 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 252 183 141 48 7 aact:AA01563|5end_transketolase_isozyme_1_1046969..1047199_POSITIVE 250 170 122 84 31 aact:AA00140|5end_hypothetical_protein_100646..100876_POSITIVE 248 155 113 63 25 aact:AA01233|5end_50S_ribosomal_protein_L6_840853..841083_POSITIVE 241 157 111 157 108 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 238 160 111 90 37 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 237 143 103 207 174 aact:AA01725|5end_hypothetical_protein_1167212..1167442_POSITIVE 236 159 136 64 39 aact:AA01673|5end_riboflavin_synthase,_alpha_chain_1131477..1131707_POSITIVE 235 157 115 199 161 aact:AA00335|5end_PTS_fructose-specific_IIBC_component_226489..226719_POSITIVE 233 144 104 186 140 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 229 149 105 170 113 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 228 166 121 58 10 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 227 35 2 197 167 aact:AA01400|5end_phosphopantetheine_adenylyltransferase_945679..945909_POSITIVE 225 147 108 227 187 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 225 147 108 85 45 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 223 159 113 174 119 aact:AA02715|5end_paraquat-inducible_protein_pqiB_homolog_1897053..1897283_POSITIVE 220 160 135 77 48 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 220 151 111 90 54 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 220 186 141 200 157 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 283 183 141 70 27 aact:AA01577|3end_conserved_hypothetical_transmembrane_protein_1064240..1064390_POSITIVE 248 155 113 44 6 aact:AA01232|3end_30S_ribosomal_protein_S8_840914..841064_POSITIVE 241 157 111 142 93 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 237 143 103 137 104 aact:AA01724|3end_valyl-tRNA_synthetase_1167222..1167372_POSITIVE 227 35 2 129 99 aact:AA01398|3end_KDO_kinase_945691..945841_POSITIVE 225 147 108 133 93 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 222 159 115 59 22 aact:AA01441|3end_tRNA/rRNA_methylase_967643..967793_POSITIVE 220 160 135 68 39 aact:AA02523|3end_conserved_hypothetical_protein_1753384..1753534_POSITIVE 219 158 113 101 59 aact:AA01370|3end_rod_shape-determining_protein_926586..926736_POSITIVE 215 166 140 74 45 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 214 145 102 43 7 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 214 190 142 125 81 aact:AA02790|3end_NAD-dependent_aldehyde_dehydrogenase_1959456..1959606_POSITIVE 214 185 147 127 90 aact:AA01264|3end_conserved_hypothetical_protein_862056..862206_POSITIVE 213 73 51 78 57 aact:AA02393|3end_conserved_hypothetical_protein;_possible_sulfate_transporter_1644262..1644412_POSITIVE 210 172 132 77 34 aact:AA00028|3end_conserved_hypothetical_protein_(possible_type_III_restriction-modification_system_restriction_subunit)_23293..23443_POSITIVE 209 157 122 57 10 aact:AA01216|3end_conserved_hypothetical_protein_832709..832859_POSITIVE 208 169 121 62 4 aact:AA01324|3end_50S_ribosomal_protein_L33_894742..894892_POSITIVE 207 165 135 148 110 aact:AA00137|3end_conserved_hypothetical_protein_(possible_alpha_helix_protein)_99057..99207_POSITIVE 205 176 141 91 56 aact:AA02699|3end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1883159..1883309_POSITIVE 205 121 95 62 38 aact:AA02316|3end_exodeoxyribonuclease_VII_large_subunit_1583093..1583243_POSITIVE