Origin IGS: agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatggttattttttctacaatgttaagatgaatgaggggtgattataacaacttattcggcttgtgcaatgagggggatttactgccgtcgttatatcggaaagaaaaaatattggaagtacttcagaagatgtaaaagacagttttaatcatatataagtatttgggggtggcgccaattctcaggtaaaatcacaattttattctttttcgattaactct Mask Tandem Repeat Region ================================================ agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Find is-nt database================================================ Query_seq: AA00280:AA00281|AA00280:AA00281:adenosylmethionine-8-amino-7-oxononanoate; 7,8-diamino-perlargonic acid ATase:phosphatidylserine decarboxylase proenzyme:->->:197857..198078 222 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00280:AA00281|AA00280:AA00281:adenosylmethionine-8-amino-7-oxononanoate; 7,8-diamino-perlargonic acid ATase:phosphatidylserine decarboxylase proenzyme:->->:197857..198078 222 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00280:AA00281|AA00280:AA00281:adenosylmethionine-8-amino-7-oxononanoate; 7,8-diamino-perlargonic acid ATase:phosphatidylserine decarboxylase proenzyme:->->:197857..198078 222 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 80 End: 205 ............................................................................... M S F T S S E V L P I F F L S D I T T A V N P P H C T S R I S C Y N H P S F I L T L ................. Protein_Len: 34 Strand: - Start: 90 End: 191 ......................................................................................... M K Q L V E L I K E K R Y L S P L L D G E N C L G F L N N Y D G R M ............................... Protein_Len: 36 Strand: - Start: 77 End: 184 ............................................................................ F Q R K C R R F Y K W Y K K K G I Y R R C Y I G R M A C A S Y T T I I M ...................................... agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00280:AA00281|AA00280:AA00281:adenosylmethionine-8-amino-7-oxononanoate; 7,8-diamino-perlargonic acid ATase:phosphatidylserine decarboxylase proenzyme:->->:197857..198078 222 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Intra-Species Hit: Count: 1 Min: 1 Max: 222 Len: 222 Subject: aact_AA00280_AA00281|adenosylmethionine-8-amino-7-oxononanoate; 7,8-diamino-perlargonic acid ATase:phosphatidylserine decarboxylase proenzyme|POSITIVE:POSITIVE|[197857,198078]|222 HSP 1 e-value: 1.0E-123 bit: 440.0 Len: 222 Query Start:1 Query End:222 Subject Strand: POSITIVE Subject Start: 1 Subject End: 222 agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt agagttaatcgaaaaagaataaaattgtgattttacctgagaattggcgccacccccaaatacttatatatgattaaaactgtcttttacatcttctgaagtacttccaatattttttctttccgatataacgacggcagtaaatccccctcattgcacaagccgaataagttgttataatcacccctcattcatcttaacattgtagaaaaaataaccatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGUUAAUCGAAAAAGAAUAAAAUUGUGAUUUUACCUGAGAAUUGGCGCCACCCCCAAAUACUUAUAUAUGAUUAAAACUGUCUUUUACAUCUUCUGAAGUACUUCCAAUAUUUUUUCUUUCCGAUAUAACGACGGCAGUAAAUCCCCCUCAUUGCACAAGCCGAAUAAGUUGUUAUAAUCACCCCUCAUUCAUCUUAACAUUGUAGAAAAAAUAACCAUU .(((....(((..((((((.(((((((.((...(((.(.(((.((((........))))............((((((((.....))))).))).))).).)))..)))))).))).)))))).)))(((((((((.(((((...........))))).............))))))))).......)))................................. (-26.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGGUUAUUUUUUCUACAAUGUUAAGAUGAAUGAGGGGUGAUUAUAACAACUUAUUCGGCUUGUGCAAUGAGGGGGAUUUACUGCCGUCGUUAUAUCGGAAAGAAAAAAUAUUGGAAGUACUUCAGAAGAUGUAAAAGACAGUUUUAAUCAUAUAUAAGUAUUUGGGGGUGGCGCCAAUUCUCAGGUAAAAUCACAAUUUUAUUCUUUUUCGAUUAACUCU .(((((((..((..((.((.(((((((.((((((((..((.......))..)))))))).)))).))).)).))..))..))..))))).((((.(((((((((((.(((.((((((..(((((.(((.(((.(((((.....))))))))............((((.(....).)))).))).)))))...)).))))))).))).))))))))))))... (-50.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 178 131 66 15 aact:AA01878|5end_hydroxyacylglutathione_hydrolase_1264831..1265061_POSITIVE 321 170 122 63 8 aact:AA00309|5end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_209285..209515_POSITIVE 312 174 131 175 126 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 280 165 123 186 130 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 280 167 124 84 34 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 277 165 132 230 197 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 277 157 117 69 30 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 276 165 123 60 21 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 275 158 119 87 44 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 273 166 124 45 1 aact:AA02973|5end_tyrosine-specific_transport_protein_2077707..2077937_POSITIVE 273 168 122 120 78 aact:AA01654|5end_periplasmic_maltose-binding_protein_precursor_1119896..1120126_POSITIVE 270 60 27 146 105 aact:AA02692|5end_hydrogenase_4_subunit;_hydrogenase_3_subunit_1876324..1876554_POSITIVE 270 164 121 147 106 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 269 165 132 199 161 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 266 166 121 183 119 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 265 166 123 60 7 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 262 178 136 228 186 aact:AA02516|5end_ferric_uptake_regulation_protein_1749264..1749494_POSITIVE 262 169 121 60 14 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 261 59 26 184 150 aact:AA02142|5end_outer_membrane_protein_OprM_precursor_1466037..1466267_POSITIVE 261 149 104 231 186 aact:AA00312|5end_conserved_hypothetical_protein_(possible_hydroxypyruvate_isomerase)_212069..212299_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 178 134 45 1 aact:AA01877|3end_conserved_hypothetical_protein_1264890..1265040_POSITIVE 280 167 124 51 1 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE 273 168 122 148 106 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 270 60 27 57 16 aact:AA02691|3end_hypothetical_protein_1876315..1876465_POSITIVE 270 164 121 147 106 aact:AA00892|3end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_619959..620109_POSITIVE 264 167 135 75 39 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 262 169 121 58 12 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE 253 163 121 39 3 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 253 165 121 54 9 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 253 167 121 141 104 aact:AA00290|3end_conserved_hypothetical_protein_201813..201963_POSITIVE 252 178 131 91 33 aact:AA01325|3end_formamidopyrimidine-DNA_glycosylase_895639..895789_POSITIVE 246 168 123 54 1 aact:AA01866|3end_thiamin_ABC_transporter,_permease_protein_1254946..1255096_POSITIVE 245 166 133 110 80 aact:AA01140|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_782526..782676_POSITIVE 243 163 121 151 116 aact:AA01782|3end_molybdenum_transport_protein;_transcriptional_regulator_1203735..1203885_POSITIVE 242 188 147 89 48 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 241 166 123 113 50 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 240 174 132 144 110 aact:AA00747|3end_UTP--hexose-1-phosphate_uridylyltransferase_/_UDPglucose-hexose-1-phosphate_uridylyltransferas_513504..513654_POSITIVE 236 156 111 136 89 aact:AA01601|3end_PTS_system,_glucose-specific_IIA_componen_1082846..1082996_POSITIVE 236 166 123 34 2 aact:AA00899|3end_possible_quaternary_ammonium_compound_resistance_protein_623479..623629_POSITIVE 234 168 123 54 7 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE