Origin IGS: taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagcaaactaaagtgcggtgagaaaatgatgtgttttttaaccgcactttttagacgataaccttaaacaaaaagccgcttttgatgatgtcaaaagcggctttttcaaggtaaagtgcggtggaaatta Mask Tandem Repeat Region ================================================ taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Find is-nt database================================================ Query_seq: AA00265:AA00266|AA00265:AA00266:transcription termination factor:conserved hypothetical protein (probable metabolite transport protein):->->:186644..186773 130 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00265:AA00266|AA00265:AA00266:transcription termination factor:conserved hypothetical protein (probable metabolite transport protein):->->:186644..186773 130 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00265:AA00266|AA00265:AA00266:transcription termination factor:conserved hypothetical protein (probable metabolite transport protein):->->:186644..186773 130 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Predict ORF larger than 30AA ================================================ taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -16.3 Tail_Score: -3.70068 Start: 24 End: 62 Full_Region: ACCGCACTTTACCTT GAAAAAGCCGCTTTTGA CATCA TCAAAAGCGGCTTTTTG TTTAAGGTTATCGTC .......................GAAAAAGCCGCTTTTGACATCATCAAAAGCGGCTTTTTG.................................................................... TransTerm Strand: - Conf: 100 HP_score: -16.0 Tail_Score: -4.59773 Start: 30 End: 56 Full_Region: aaccttaaacaaaaa gccgcttttgat gat gtcaaaagcggc tttttcaaggtaaag .............................gccgcttttgatgatgtcaaaagcggc.......................................................................... Find igs database================================================ Query_seq: AA00265:AA00266|AA00265:AA00266:transcription termination factor:conserved hypothetical protein (probable metabolite transport protein):->->:186644..186773 130 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: aact_AA00265_AA00266|transcription termination factor:conserved hypothetical protein (probable metabolite transport protein)|POSITIVE:POSITIVE|[186644,186773]|130 HSP 1 e-value: 4.0E-68 bit: 258.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta taatttccaccgcactttaccttgaaaaagccgcttttgacatcatcaaaagcggctttttgtttaaggttatcgtctaaaaagtgcggttaaaaaacacatcattttctcaccgcactttagtttgcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUCCACCGCACUUUACCUUGAAAAAGCCGCUUUUGACAUCAUCAAAAGCGGCUUUUUGUUUAAGGUUAUCGUCUAAAAAGUGCGGUUAAAAAACACAUCAUUUUCUCACCGCACUUUAGUUUGCUA ...........(((....((((((((((((((((((((((.....))))))))))))))...))))))))..........((((((((((..((((........))))...))))))))))....))).. (-43.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCAAACUAAAGUGCGGUGAGAAAAUGAUGUGUUUUUUAACCGCACUUUUUAGACGAUAACCUUAAACAAAAAGCCGCUUUUGAUGAUGUCAAAAGCGGCUUUUUCAAGGUAAAGUGCGGUGGAAAUUA ..(((..((((((((((((.(((((((.....))))))).))))))))..))))......(((((....(((((((((((((((((...))))))))))))))))).)))))....)))........... (-49.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 482 130 81 88 39 aact:AA02213|5end_hypothetical_protein_1506996..1507226_POSITIVE 470 120 78 93 51 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 460 130 81 71 13 aact:AA00697|5end_hypothetical_protein_476999..477229_POSITIVE 426 121 81 188 146 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 426 120 74 80 32 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 426 126 81 99 54 aact:AA00235|5end_hypothetical_protein_168596..168826_POSITIVE 423 129 81 81 33 aact:AA02037|5end_hypothetical_protein_1386832..1387062_POSITIVE 421 121 81 154 114 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 419 120 79 140 99 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 418 128 81 217 172 aact:AA01625|5end_riboflavin_synthase,_beta_chain_1099964..1100194_POSITIVE 413 120 75 74 27 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 410 126 81 161 116 aact:AA00473|5end_tryptophanyl-tRNA_synthetase_328996..329226_POSITIVE 388 125 81 205 159 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 388 125 81 52 6 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 379 122 81 136 95 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 378 129 81 192 144 aact:AA02722|5end_cytidine_deaminase_1905379..1905609_POSITIVE 378 130 81 225 172 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 373 120 71 199 150 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 370 130 81 155 106 aact:AA00825|5end_P-protein_(chorismate_mutase/prephenate_dehydratase)_567020..567250_POSITIVE 370 130 81 55 6 aact:AA00824|5end_hypothetical_protein_566920..567150_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 534 60 11 65 3 aact:AA00265|3end_transcription_termination_factor_186583..186733_POSITIVE 482 130 81 90 41 aact:AA02212|3end_ribonucleoside-diphosphate_reductase_beta_chain_1507078..1507228_POSITIVE 470 120 78 73 31 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 460 130 81 98 40 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 426 121 81 84 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 426 126 81 64 19 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 423 129 81 74 26 aact:AA02035|3end_translation_initiation_factor_IF-3_1386905..1387055_POSITIVE 421 121 81 75 35 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 418 128 81 74 29 aact:AA01624|3end_glucose-6-phosphate_isomerase_1099901..1100051_POSITIVE 410 126 81 68 23 aact:AA00471|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328983..329133_POSITIVE 405 120 80 148 107 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE 395 129 81 92 46 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 379 122 81 64 23 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 378 129 81 67 19 aact:AA02721|3end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1905334..1905484_POSITIVE 373 120 71 98 49 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 367 127 81 85 41 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 365 128 81 64 17 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 359 120 76 70 24 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 359 122 81 141 95 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 349 120 80 72 27 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE