Origin IGS: gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgacttttaaagtgcggtgaaattttgtggtattttctataaaaaaacactcaaaaaaataaccgcacttttctagaataacgctcaaataaatggggtccccgtataagacgcctgagcgac Mask Tandem Repeat Region ================================================ gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Find is-nt database================================================ Query_seq: AA00237:AA00239|AA00237:AA00239:hypothetical protein:hypothetical protein:->->:171230..171352 123 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00237:AA00239|AA00237:AA00239:hypothetical protein:hypothetical protein:->->:171230..171352 123 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00237:AA00239|AA00237:AA00239:hypothetical protein:hypothetical protein:->->:171230..171352 123 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 30 End: 122 ............................. M Y L S V I L E K C G Y F F E C F F I E N T T K F H R T L K V . gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00237:AA00239|AA00237:AA00239:hypothetical protein:hypothetical protein:->->:171230..171352 123 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggttatttttttgagtgtttttttatagaaaataccacaaaatttcaccgcactttaaaagtca Intra-Species Hit: Count: 1 Min: 1 Max: 123 Len: 123 Subject: aact_AA00237_AA00239|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[171230,171352]|123 HSP 1 e-value: 9.0E-29 bit: 127.0 Len: 64 Query Start:1 Query End:64 Subject Strand: POSITIVE Subject Start: 1 Subject End: 64 gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggtta........................................................... gtcgctcaggcgtcttatacggggaccccatttatttgagcgttattctagaaaagtgcggtta........................................................... HSP 2 e-value: 2.0E-14 bit: 79.8 Len: 40 Query Start:84 Query End:123 Subject Strand: POSITIVE Subject Start: 84 Subject End: 123 ...................................................................................atagaaaataccacaaaatttcaccgcactttaaaagtca ...................................................................................atagaaaataccacaaaatttcaccgcactttaaaagtca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCGCUCAGGCGUCUUAUACGGGGACCCCAUUUAUUUGAGCGUUAUUCUAGAAAAGUGCGGUUAUUUUUUUGAGUGUUUUUUUAUAGAAAAUACCACAAAAUUUCACCGCACUUUAAAAGUCA ..((((((((.(((((.....))))).)).......))))))..........((((((((((...((((.((.((((((((.....)))))))))).))))....))))))))))........ (-35.81) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACUUUUAAAGUGCGGUGAAAUUUUGUGGUAUUUUCUAUAAAAAAACACUCAAAAAAAUAACCGCACUUUUCUAGAAUAACGCUCAAAUAAAUGGGGUCCCCGUAUAAGACGCCUGAGCGAC ...((...((((((((((....(((((.(((.((((.....)))).)).).))))).....))))))))))...)).....((((((.......((.(((.........))).)))))))).. (-33.51) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 576 59 15 135 93 aact:AA02490|5end_hypothetical_protein_1726610..1726840_POSITIVE 461 36 2 157 126 aact:AA00658|5end_hypothetical_protein_451618..451848_POSITIVE 404 49 1 231 184 aact:AA01814|5end_endonuclease_III_1221881..1222111_POSITIVE 391 80 35 122 65 aact:AA00854|5end_arginine_transport_system_permease_protein_588898..589128_POSITIVE 374 81 50 176 145 aact:AA01686|5end_hypothetical_protein_1137913..1138143_POSITIVE 352 80 40 209 172 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 351 82 53 171 142 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 350 82 54 155 127 aact:AA00308|5end_glycerol-3-phosphate_regulon_repressor_208337..208567_POSITIVE 336 81 42 223 183 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 333 86 52 163 127 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 329 80 34 180 135 aact:AA01901|5end_hypothetical_protein_1282593..1282823_POSITIVE 326 80 31 121 72 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 326 80 35 169 127 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 324 82 41 173 131 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 322 115 85 112 82 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 320 80 40 154 114 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 320 80 40 78 38 aact:AA02835|5end_hypothetical_protein_1991701..1991931_POSITIVE 319 115 71 180 124 aact:AA00561|5end_DNA_transformation_protein_384584..384814_POSITIVE 318 82 51 132 100 aact:AA01364|5end_hypothetical_protein_922661..922891_POSITIVE 316 80 35 175 131 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 677 60 11 95 46 aact:AA00570|3end_hypothetical_protein_391785..391935_POSITIVE 576 59 15 74 32 aact:AA02489|3end_hypothetical_protein_1726629..1726779_POSITIVE 441 47 1 135 94 aact:AA00042|3end_hypothetical_protein_34255..34405_POSITIVE 404 49 1 81 34 aact:AA01813|3end_electron_transport_complex_protein_1221811..1221961_POSITIVE 374 81 50 99 68 aact:AA01685|3end_molybdenum_ABC_transporter,_ATP-binding_protein_1137916..1138066_POSITIVE 366 87 42 63 12 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 352 80 40 85 48 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 351 82 53 131 102 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 350 82 54 76 48 aact:AA00306|3end_nitrogen_regulatory_protein_p-II_208338..208488_POSITIVE 341 80 35 117 74 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 341 80 35 133 90 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 336 81 42 89 49 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 329 80 34 89 44 aact:AA01900|3end_hypothetical_protein_1282582..1282732_POSITIVE 326 80 35 88 46 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 324 82 41 86 44 aact:AA02091|3end_hypothetical_protein_1424667..1424817_POSITIVE 322 115 85 131 101 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 320 80 40 65 25 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 319 115 71 107 51 aact:AA00560|3end_RNA_polymerase_sigma-32_factor_384591..384741_POSITIVE 318 82 51 128 96 aact:AA01363|3end_hypothetical_protein_922737..922887_POSITIVE 316 80 35 81 37 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE