Origin IGS: taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cttatgttttcctttttgttagggtaaattacattgataattaatatgttaataggttctgcagcttaaaaatgtagattaaggcgttataaattagatttggtacttgattatattaatggagttttattggctaatatttgtgcaagactgagttaagggggtacatcctaactatataaattttaaatgcaagtaaattttcattgtttta Mask Tandem Repeat Region ================================================ taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Find is-nt database================================================ Query_seq: AA00233:AA00234|AA00233:AA00234:hypothetical protein:collagen adhesin:->->:162593..162806 214 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00233:AA00234|AA00233:AA00234:hypothetical protein:collagen adhesin:->->:162593..162806 214 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00233:AA00234|AA00233:AA00234:hypothetical protein:collagen adhesin:->->:162593..162806 214 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Predict ORF larger than 30AA ================================================ taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00233:AA00234|AA00233:AA00234:hypothetical protein:collagen adhesin:->->:162593..162806 214 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Intra-Species Hit: Count: 1 Min: 1 Max: 214 Len: 214 Subject: aact_AA00233_AA00234|hypothetical protein:collagen adhesin|POSITIVE:POSITIVE|[162593,162806]|214 HSP 1 e-value: 1.0E-118 bit: 424.0 Len: 214 Query Start:1 Query End:214 Subject Strand: POSITIVE Subject Start: 1 Subject End: 214 taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag taaaacaatgaaaatttacttgcatttaaaatttatatagttaggatgtacccccttaactcagtcttgcacaaatattagccaataaaactccattaatataatcaagtaccaaatctaatttataacgccttaatctacatttttaagctgcagaacctattaacatattaattatcaatgtaatttaccctaacaaaaaggaaaacataag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACAAUGAAAAUUUACUUGCAUUUAAAAUUUAUAUAGUUAGGAUGUACCCCCUUAACUCAGUCUUGCACAAAUAUUAGCCAAUAAAACUCCAUUAAUAUAAUCAAGUACCAAAUCUAAUUUAUAACGCCUUAAUCUACAUUUUUAAGCUGCAGAACCUAUUAACAUAUUAAUUAUCAAUGUAAUUUACCCUAACAAAAAGGAAAACAUAAG .......(((.........(((((((.(.((((.((((.((((((.........))))))...((..((((............................(((((...((........))...)))))....(((((.........))))).))))..))........)))).)))).).))))))).....(((.......)))....)))... (-17.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUAUGUUUUCCUUUUUGUUAGGGUAAAUUACAUUGAUAAUUAAUAUGUUAAUAGGUUCUGCAGCUUAAAAAUGUAGAUUAAGGCGUUAUAAAUUAGAUUUGGUACUUGAUUAUAUUAAUGGAGUUUUAUUGGCUAAUAUUUGUGCAAGACUGAGUUAAGGGGGUACAUCCUAACUAUAUAAAUUUUAAAUGCAAGUAAAUUUUCAUUGUUUUA .....((((.((((......)))).)))).(((.(((.((((...............((((((.........)))))).....(((((..(((((.((((..((.((((...((((((.(.((......)).).)))))).....))))))..))))..((((.....)))).........)))))..))))).....)))).))).))).... (-33.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 250 72 44 203 175 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 235 100 54 163 123 aact:AA01726|5end_conserved_hypothetical_protein_(possible_HipA-like_protein_1168105..1168335_POSITIVE 227 88 44 144 112 aact:AA02595|5end_cytochrome_c_peroxidase_1805741..1805971_POSITIVE 223 84 44 122 90 aact:AA01727|5end_conserved_hypothetical_protein_(possible_HipA_protein)_1168227..1168457_POSITIVE 218 170 129 152 111 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 217 87 51 149 113 aact:AA01885|5end_hydrogenase_nickel_incorporation_protein_1271117..1271347_POSITIVE 215 154 117 185 151 aact:AA01725|5end_hypothetical_protein_1167212..1167442_POSITIVE 214 90 43 62 17 aact:AA01690|5end_penicillin-binding_protein_1C_1146708..1146938_POSITIVE 213 157 114 127 86 aact:AA00371|5end_transcriptional_regulator_249181..249411_POSITIVE 210 158 127 197 166 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 210 72 39 221 187 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 206 83 44 231 200 aact:AA01728|5end_conserved_hypothetical_protein(related_to_HipA_protein)_1168337..1168567_POSITIVE 202 86 44 69 25 aact:AA02260|5end_pseudouridylate_synthase_I_1537067..1537297_POSITIVE 200 160 117 173 131 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 200 163 129 203 163 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 199 85 44 45 1 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 195 85 53 180 144 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 193 155 117 213 180 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 193 94 53 210 166 aact:AA01477|5end_type_I_restriction_enzyme_R_protein_992249..992479_POSITIVE 192 99 52 165 106 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 223 84 44 119 87 aact:AA01726|3end_conserved_hypothetical_protein_(possible_HipA-like_protein_1168304..1168454_POSITIVE 219 83 46 123 91 aact:AA00061|3end_cytochrome_c-type_biogenesis_ATP-binding_protein_46794..46944_POSITIVE 218 155 113 149 95 aact:AA01603|3end_oligopeptidase_A_1085014..1085164_POSITIVE 218 170 129 146 105 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 217 87 51 139 103 aact:AA01883|3end_recycling_protein;_permease_1271187..1271337_POSITIVE 217 83 54 151 120 aact:AA01725|3end_hypothetical_protein_1168182..1168332_POSITIVE 215 154 117 115 81 aact:AA01724|3end_valyl-tRNA_synthetase_1167222..1167372_POSITIVE 204 75 51 29 7 aact:AA02416|3end_conserved_hypothetical_protein_1659578..1659728_POSITIVE 202 86 44 50 6 aact:AA02259|3end_possible_RelE-like_protein_1537128..1537278_POSITIVE 200 160 117 128 86 aact:AA02486|3end_topoisomerase_IV,_subunit_B_1726312..1726462_POSITIVE 198 94 51 135 88 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 193 155 117 127 94 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 193 94 53 142 98 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 193 162 128 49 7 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 190 165 129 151 118 aact:AA01383|3end_DNA_processing_chain_A_935366..935516_POSITIVE 189 167 130 134 97 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 188 94 58 135 95 aact:AA00527|3end_ATP-dependent_DNA_helicase_361463..361613_POSITIVE 187 168 128 151 100 aact:AA01432|3end_lysophospholipase_L2_961766..961916_POSITIVE 187 69 40 132 100 aact:AA01152|3end_sec-independent_protein_translocase_protein_790205..790355_POSITIVE 183 209 175 86 52 aact:AA02808|3end_conserved_hypothetical_protein_(possible_serine_hydroxymethyltransferase)_1977944..1978094_POSITIVE