Origin IGS: taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatcgtttccttaatttttgagtagtagagtaaaaaatatccattttcatggatgatcgattgacagaaattataaataccagcaaaaagttctcaatctattaaattaataatatttgacgattaaaaaataactattaatagttagaattgatttaatcaagaaaaaagaaatgagaaaagaaaatgatcgcactttgatctgcaccctaaaaccgggcggatagctaaacctaagttaaatattgagctctgcactgcacagggctcaaattgtttaacttaagcttaattacgctcctcaataataacgaatatattcatcgtccgcccgggttcatcatgtagttaaaccggcgggaataaaaatattccgattaaataatgttttcctataaaaacctgcattttaattgattggcggcggcgtcgtctttgggagggcagatcaaagtgcggtcattttcaacggtgttttttctgaccgatta Mask Tandem Repeat Region ================================================ taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Find is-nt database================================================ Query_seq: AA00221:AA00223|AA00221:AA00223:NADP-specific glutamate dehydrogenase:catalase (hydroperoxidase; iron heme axial ligand):->->:153926..154416 491 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00221:AA00223|AA00221:AA00223:NADP-specific glutamate dehydrogenase:catalase (hydroperoxidase; iron heme axial ligand):->->:153926..154416 491 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00221:AA00223|AA00221:AA00223:NADP-specific glutamate dehydrogenase:catalase (hydroperoxidase; iron heme axial ligand):->->:153926..154416 491 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 45 End: 140 ............................................ M P S Q R R R R R Q S I K M Q V F I G K H Y L I G I F L F P P V ............................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 234 End: 329 ......................................................................................................................................................................................................................................... M Q S S I F N L G L A I R P V L G C R S K C D H F L F S F L F S .................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 284 End: 382 ........................................................................................................................................................................................................................................................................................... M Q I K V R S F S F L I S F F L I K S I L T I N S Y F L I V K Y Y ............................................................................................................. Protein_Len: 32 Strand: + Start: 394 End: 489 ......................................................................................................................................................................................................................................................................................................................................................................................................... M E N F L L V F I I S V N R S S M K M D I F Y S T T Q K L R K R .. Protein_Len: 31 Strand: - Start: 147 End: 239 .................................................................................................................................................. M I F G P P R H I Y E N N N L L T I L S L N F L K L G T C H M ............................................................................................................................................................................................................................................................ taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 63 HP_score: -5.3 Tail_Score: -4.0098 Start: 219 End: 247 Full_Region: aaacctaagttaaat attgagctctg cactgca cagggctcaaa ttgtttaacttaagc ..........................................................................................................................................................................................................................attgagctctgcactgcacagggctcaaa.................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 72 HP_score: -8.0 Tail_Score: -3.47184 Start: 220 End: 246 Full_Region: CTTAAGTTAAACAAT TTGAGCCCTGTG CAG TGCAGAGCTCAA TATTTAACTTAGGTT ...........................................................................................................................................................................................................................TTGAGCCCTGTGCAGTGCAGAGCTCAA..................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 71 HP_score: -8.8 Tail_Score: -3.00106 Start: 220 End: 246 Full_Region: aacctaagttaaata ttgagctctg cactgca cagggctcaa attgtttaacttaag ...........................................................................................................................................................................................................................ttgagctctgcactgcacagggctcaa..................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 77 HP_score: -4.8 Tail_Score: -5.1861 Start: 345 End: 360 Full_Region: TGATTAAATCAATTC TAACTA TTAA TAGTTA TTTTTTAATCGTCAA ........................................................................................................................................................................................................................................................................................................................................................TAACTATTAATAGTTA................................................................................................................................... TransTerm Strand: + Conf: 72 HP_score: -2.7 Tail_Score: -5.23316 Start: 437 End: 454 Full_Region: TTTCTGTCAATCGAT CATCCAT GAAA ATGGATA TTTTTTACTCTACTA ....................................................................................................................................................................................................................................................................................................................................................................................................................................................CATCCATGAAAATGGATA..................................... Find igs database================================================ Query_seq: AA00221:AA00223|AA00221:AA00223:NADP-specific glutamate dehydrogenase:catalase (hydroperoxidase; iron heme axial ligand):->->:153926..154416 491 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatcattttcttttctcatttcttttttcttgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Intra-Species Hit: Count: 1 Min: 1 Max: 491 Len: 491 Subject: aact_AA00221_AA00223|NADP-specific glutamate dehydrogenase:catalase (hydroperoxidase; iron heme axial ligand)|POSITIVE:POSITIVE|[153926,154416]|491 HSP 1 e-value: 1.0E-171 bit: 603.0 Len: 304 Query Start:1 Query End:304 Subject Strand: POSITIVE Subject Start: 1 Subject End: 304 taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatca........................................................................................................................................................................................... taatcggtcagaaaaaacaccgttgaaaatgaccgcactttgatctgccctcccaaagacgacgccgccgccaatcaattaaaatgcaggtttttataggaaaacattatttaatcggaatatttttattcccgccggtttaactacatgatgaacccgggcggacgatgaatatattcgttattattgaggagcgtaattaagcttaagttaaacaatttgagccctgtgcagtgcagagctcaatatttaacttaggtttagctatccgcccggttttagggtgcagatcaaagtgcgatca........................................................................................................................................................................................... HSP 2 e-value: 1.0E-86 bit: 321.0 Len: 162 Query Start:330 Query End:491 Subject Strand: POSITIVE Subject Start: 330 Subject End: 491 .........................................................................................................................................................................................................................................................................................................................................tgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt .........................................................................................................................................................................................................................................................................................................................................tgattaaatcaattctaactattaatagttattttttaatcgtcaaatattattaatttaatagattgagaactttttgctggtatttataatttctgtcaatcgatcatccatgaaaatggatattttttactctactactcaaaaattaaggaaacgatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGGUCAGAAAAAACACCGUUGAAAAUGACCGCACUUUGAUCUGCCCUCCCAAAGACGACGCCGCCGCCAAUCAAUUAAAAUGCAGGUUUUUAUAGGAAAACAUUAUUUAAUCGGAAUAUUUUUAUUCCCGCCGGUUUAACUACAUGAUGAACCCGGGCGGACGAUGAAUAUAUUCGUUAUUAUUGAGGAGCGUAAUUAAGCUUAAGUUAAACAAUUUGAGCCCUGUGCAGUGCAGAGCUCAAUAUUUAACUUAGGUUUAGCUAUCCGCCCGGUUUUAGGGUGCAGAUCAAAGUGCGAUCAUUUUCUUUUCUCAUUUCUUUUUUCUUGAUUAAAUCAAUUCUAACUAUUAAUAGUUAUUUUUUAAUCGUCAAAUAUUAUUAAUUUAAUAGAUUGAGAACUUUUUGCUGGUAUUUAUAAUUUCUGUCAAUCGAUCAUCCAUGAAAAUGGAUAUUUUUUACUCUACUACUCAAAAAUUAAGGAAACGAUU ..(((((((((((((((.(((...((((((((.(((((((((((((((...(((.(((((.((((..((..((((......(((((...((((((....))))))..))))).....((((((.((((((.(((((((((((.(.....).))))))..)))))..)))))).))))))......)))).)).))))..((((((((((((((((....((((((.((((.....)))).))))))..))))))))))))))))...........))))).))).))))))))))))))).))))))))..((((((.............(((((((.......((((((....))))))...)))))))......(((((......)))))...)))))).........))).)))....))))))...)))))).((((((....))))))............................(....).... (-143.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCGUUUCCUUAAUUUUUGAGUAGUAGAGUAAAAAAUAUCCAUUUUCAUGGAUGAUCGAUUGACAGAAAUUAUAAAUACCAGCAAAAAGUUCUCAAUCUAUUAAAUUAAUAAUAUUUGACGAUUAAAAAAUAACUAUUAAUAGUUAGAAUUGAUUUAAUCAAGAAAAAAGAAAUGAGAAAAGAAAAUGAUCGCACUUUGAUCUGCACCCUAAAACCGGGCGGAUAGCUAAACCUAAGUUAAAUAUUGAGCUCUGCACUGCACAGGGCUCAAAUUGUUUAACUUAAGCUUAAUUACGCUCCUCAAUAAUAACGAAUAUAUUCAUCGUCCGCCCGGGUUCAUCAUGUAGUUAAACCGGCGGGAAUAAAAAUAUUCCGAUUAAAUAAUGUUUUCCUAUAAAAACCUGCAUUUUAAUUGAUUGGCGGCGGCGUCGUCUUUGGGAGGGCAGAUCAAAGUGCGGUCAUUUUCAACGGUGUUUUUUCUGACCGAUUA (((((................................(((((((....))))))).........((((((....(((((((.........((((((.((((((((((((((....((((((.(((((..........))))).))))))))))))))))).........)))..))))))..((((((((((((((((((((((((.((((((..((((((((((.(((.....(((((((((((((((((((((.......))))))))))..))))))))))))))........................(((....)))...))))))))))..........((((((((...((.((((((....))))))...........(((((......)))))..))..))))))))...((((((...)))))).))))).).))))))))))))))))))))))))...)))))))))))))..))))). (-148.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 833 260 211 96 47 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 543 250 209 61 21 aact:AA00467|5end_hypothetical_protein_324565..324795_POSITIVE 540 80 31 151 101 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 475 50 5 83 42 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 458 56 13 141 97 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 452 69 21 94 38 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 450 270 221 100 57 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 450 270 221 100 57 aact:AA01287|5end_transposase_871583..871813_POSITIVE 447 63 30 137 104 aact:AA00536|5end_5'-nucleotidase_precursor_366151..366381_POSITIVE 443 50 5 159 115 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 443 60 31 176 143 aact:AA01407|5end_outer_membrane_antigenic_lipoprotein_b_precursor_949005..949235_POSITIVE 443 60 31 178 145 aact:AA00632|5end_inner_membrane_protein_ImpA_434127..434357_POSITIVE 434 66 21 147 98 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 420 319 271 92 43 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 419 311 273 231 191 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 419 308 273 137 101 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 413 319 273 83 27 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 411 270 221 144 101 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 408 59 12 161 116 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 408 73 31 152 125 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 575 50 5 66 23 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 452 69 21 122 66 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 450 270 221 54 11 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 434 66 21 86 37 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 420 319 271 92 43 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 419 311 273 80 40 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 419 308 273 75 39 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 413 319 273 89 33 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 408 59 12 57 12 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 405 50 2 46 3 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 403 300 269 58 28 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 401 308 273 69 33 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 400 300 254 47 5 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 400 300 254 47 5 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 399 309 270 66 24 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 391 42 5 150 115 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 391 72 31 151 125 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 387 60 12 44 1 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 385 58 13 70 8 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 382 69 21 65 11 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE