Origin IGS: tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 catcactccttttgataaattaacacgcggtgtaagtgtagaagaaatcgacaaaaagcgaaaacagataaaccgggttttgtgaagtggatcaaaaaacggcgtgtggatttgctgcttcgtttttgccgttttataagaataagttgtgctaacgggcagtttgtgaaacaattcaccgttgtttgaatttaaaagattaaaagtaaggatacgata Mask Tandem Repeat Region ================================================ tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Find is-nt database================================================ Query_seq: AA00204:AA00205|AA00204:AA00205:(di)nucleoside polyphosphate hydrolase; possible invasion protein:ribose ABC transporter protein / high affinity D-ribose transport protein:->->:142083..142301 219 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00204:AA00205|AA00204:AA00205:(di)nucleoside polyphosphate hydrolase; possible invasion protein:ribose ABC transporter protein / high affinity D-ribose transport protein:->->:142083..142301 219 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00204:AA00205|AA00204:AA00205:(di)nucleoside polyphosphate hydrolase; possible invasion protein:ribose ABC transporter protein / high affinity D-ribose transport protein:->->:142083..142301 219 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 29 End: 214 ............................ M Q T T V N C F T N C P L A Q L I L I K R Q K R S S K S T R R F L I H F T K P G L S V F A F C R F L L H L H R V L I Y Q ..... Protein_Len: 60 Strand: + Start: 7 End: 219 ...... M L T F N L L N S N N G E L F H K L P V S T T Y S Y K T A K T K Q Q I H T P F F D P L H K T R F I C F R F L S I S S T L Protein_Len: 56 Strand: - Start: 34 End: 201 ................................. V V T F Q K V F Q G N A C S I R I F R C F R L L L D V R R K K I W K V F G P K D T K A K Q R N R R C K C R T N M .................. tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00204:AA00205|AA00204:AA00205:(di)nucleoside polyphosphate hydrolase; possible invasion protein:ribose ABC transporter protein / high affinity D-ribose transport protein:->->:142083..142301 219 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Intra-Species Hit: Count: 1 Min: 1 Max: 219 Len: 219 Subject: aact_AA00204_AA00205|(di)nucleoside polyphosphate hydrolase; possible invasion protein:ribose ABC transporter protein / high affinity D-ribose transport protein|POSITIVE:POSITIVE|[142083,142301]|219 HSP 1 e-value: 1.0E-121 bit: 434.0 Len: 219 Query Start:1 Query End:219 Subject Strand: POSITIVE Subject Start: 1 Subject End: 219 tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg tatcgtatccttacttttaatcttttaaattcaaacaacggtgaattgtttcacaaactgcccgttagcacaacttattcttataaaacggcaaaaacgaagcagcaaatccacacgccgttttttgatccacttcacaaaacccggtttatctgttttcgctttttgtcgatttcttctacacttacaccgcgtgttaatttatcaaaaggagtgatg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCGUAUCCUUACUUUUAAUCUUUUAAAUUCAAACAACGGUGAAUUGUUUCACAAACUGCCCGUUAGCACAACUUAUUCUUAUAAAACGGCAAAAACGAAGCAGCAAAUCCACACGCCGUUUUUUGAUCCACUUCACAAAACCCGGUUUAUCUGUUUUCGCUUUUUGUCGAUUUCUUCUACACUUACACCGCGUGUUAAUUUAUCAAAAGGAGUGAUG ((((...(((((............................(((((....))))).........((((((((...((((....))))..(((((((((((((((((...........((((..(((((..........)))))..))))....))).))))).)))))))))......................)))))))).......)))))..)))) (-40.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUCACUCCUUUUGAUAAAUUAACACGCGGUGUAAGUGUAGAAGAAAUCGACAAAAAGCGAAAACAGAUAAACCGGGUUUUGUGAAGUGGAUCAAAAAACGGCGUGUGGAUUUGCUGCUUCGUUUUUGCCGUUUUAUAAGAAUAAGUUGUGCUAACGGGCAGUUUGUGAAACAAUUCACCGUUGUUUGAAUUUAAAAGAUUAAAAGUAAGGAUACGAUA .(((....(((((((((((((((((.((((((.....(((((((....(((.....((((((..((.((...(((..(((((..........)))))..))).)).))..))))))...)))))))))).((((((((((......((((.(....).))))))))))))))....))))))))))..)))))).....)))))))....)))...... (-48.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 69 39 107 80 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 302 150 102 51 1 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 298 198 152 138 95 aact:AA02112|5end_cell_division_protein_1437617..1437847_POSITIVE 290 147 102 219 166 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 288 122 89 61 34 aact:AA01503|5end_glycerol-3-phosphate_dehydrogenase_1010814..1011044_POSITIVE 283 140 91 87 37 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 278 135 96 229 173 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 276 121 89 38 10 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 276 120 74 69 18 aact:AA00263|5end_ATP-dependent_RNA_helicase_183690..183920_POSITIVE 275 71 40 186 150 aact:AA01567|5end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1054944..1055174_POSITIVE 275 71 40 187 153 aact:AA01262|5end_hydrolase_859576..859806_POSITIVE 271 147 103 182 135 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 271 203 161 139 88 aact:AA00871|5end_rough_colony_protein_A_602561..602791_POSITIVE 269 146 113 205 169 aact:AA00175|5end_cell_filamentation_protein_123922..124152_POSITIVE 267 158 113 225 184 aact:AA01718|5end_DNA_polymerase_III,_chi_subunit_1162790..1163020_POSITIVE 267 121 84 211 179 aact:AA01179|5end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_807996..808226_POSITIVE 266 160 114 64 19 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 265 159 115 139 102 aact:AA01197|5end_conserved_hypothetical_protein_820863..821093_POSITIVE 264 122 89 221 189 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 263 125 86 136 101 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 150 102 51 1 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 298 198 152 135 92 aact:AA02111|3end_killing_protein_suppressor_1437694..1437844_POSITIVE 288 122 89 55 28 aact:AA01502|3end_serine_acetyltransferase_1010888..1011038_POSITIVE 283 140 91 106 56 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 278 153 116 92 52 aact:AA02169|3end_hypothetical_protein_1484664..1484814_POSITIVE 276 121 89 38 10 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 275 198 156 125 80 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 274 68 33 121 79 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 271 203 161 134 83 aact:AA00870|3end_rough_colony_protein_C_602636..602786_POSITIVE 265 208 166 149 107 aact:AA02008|3end_conserved_hypothetical_protein_1366474..1366624_POSITIVE 265 159 115 151 114 aact:AA01196|3end_1-acyl-sn-glycerol-3-phosphate_acetyltransferase_820955..821105_POSITIVE 262 137 92 62 13 aact:AA00743|3end_conserved_hypothetical_protein_(possible_membrane_protein)_509947..510097_POSITIVE 262 179 131 123 71 aact:AA00634|3end_DNA_polymerase_IV_(Pol_IV)_435831..435981_POSITIVE 260 186 143 149 106 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 260 180 132 136 90 aact:AA00804|3end_fumarate_reductase_flavoprotein_subunit_A_551655..551805_POSITIVE 259 190 145 150 97 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 255 194 155 112 56 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 252 156 115 127 90 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 249 160 114 133 82 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 248 155 115 80 36 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE