Origin IGS: caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgaaaaagtgcggtcggtttttaaaatgttttttattttcaccgtgattgacgctcattttcccctcgcccgtttacgggacaaatgcttgcatttgagcgttggctctgccaacgaccccgaaggggcgagtgaagcgagtattgggtggagaaaatccgagggattttcggagggagaggggggaaataaagttcgtatcctcctctctctgacaaaaattg Mask Tandem Repeat Region ================================================ caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Find is-nt database================================================ Query_seq: AA00199:AA00200|AA00199:AA00200:hypothetical protein:thymidylate synthase:->->:138805..139031 227 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00199:AA00200|AA00199:AA00200:hypothetical protein:thymidylate synthase:->->:138805..139031 227 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00199:AA00200|AA00199:AA00200:hypothetical protein:thymidylate synthase:->->:138805..139031 227 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 35 End: 148 .................................. M S P L S L R K S L G F S P P N T R F T R P F G V V G R A N A Q M Q A F V P ............................................................................... Protein_Len: 55 Strand: + Start: 3 End: 167 .. M F V R E R R I R T L F P P S P S E N P S D F L H P I L A S L A P S G S L A E P T L K C K H L S R K R A R G K ............................................................ Protein_Len: 59 Strand: + Start: 7 End: 183 ...... M S E R G G Y E L Y F P P L P P K I P R I F S T Q Y S L H S P L R G R W Q S Q R S N A S I C P V N G R G E N E R Q S R ............................................ Protein_Len: 37 Strand: - Start: 13 End: 123 ............ L S S S V F K I E G R E R R F D R P N E G G L V R K V R G K P T T P L A M ........................................................................................................ Protein_Len: 60 Strand: - Start: 2 End: 217 . G G E G E S F G E S K R W G I S A E S A G E P D N A S G V S L H L C K D R L R A L P F H A D I V T F I F F M K F V S R M .......... caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00199:AA00200|AA00199:AA00200:hypothetical protein:thymidylate synthase:->->:138805..139031 227 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Intra-Species Hit: Count: 2 Min: 1 Max: 227 Len: 227 Subject: aact_AA00199_AA00200|hypothetical protein:thymidylate synthase|POSITIVE:POSITIVE|[138805,139031]|227 HSP 1 e-value: 1.0E-126 bit: 450.0 Len: 227 Query Start:1 Query End:227 Subject Strand: POSITIVE Subject Start: 1 Subject End: 227 caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta caatttttgtcagagagaggaggatacgaactttatttcccccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcccgtaaacgggcgaggggaaaatgagcgtcaatcacggtgaaaataaaaaacattttaaaaaccgaccgcactttttcgtta Subject: aact_AA00983_AA00985|aldo-keto reductase:transcriptional regulator (LysR family)|POSITIVE:POSITIVE|[688553,688910]|358 HSP 1 e-value: 3.0E-39 bit: 163.0 Len: 106 Query Start:41 Query End:146 Subject Strand: NEGATIVE Subject Start: 59 Subject End: 164 ........................................cccctctccctccgaaaatccctcggattttctccacccaatactcgcttcactcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcc................................................................................. ........................................cccctctccctccgagaattctgcgaattttctccacccaatactcgcttcgctcgccccttcggggtcgttggcagagccaacgctcaaatgcaagcatttgtcc................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAUUUUUGUCAGAGAGAGGAGGAUACGAACUUUAUUUCCCCCCUCUCCCUCCGAAAAUCCCUCGGAUUUUCUCCACCCAAUACUCGCUUCACUCGCCCCUUCGGGGUCGUUGGCAGAGCCAACGCUCAAAUGCAAGCAUUUGUCCCGUAAACGGGCGAGGGGAAAAUGAGCGUCAAUCACGGUGAAAAUAAAAAACAUUUUAAAAACCGACCGCACUUUUUCGUUA ............(((((((..((....((..(((((((..((((((.(((...((((((((...))))))))...........((((((.....((.(((....))).))..))).)))...(((..((((((....))))))...)))....))).)))))).)))))))..)).....((((.(((((.......)))))....)))).))...))))))).... (-67.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAAAAAGUGCGGUCGGUUUUUAAAAUGUUUUUUAUUUUCACCGUGAUUGACGCUCAUUUUCCCCUCGCCCGUUUACGGGACAAAUGCUUGCAUUUGAGCGUUGGCUCUGCCAACGACCCCGAAGGGGCGAGUGAAGCGAGUAUUGGGUGGAGAAAAUCCGAGGGAUUUUCGGAGGGAGAGGGGGGAAAUAAAGUUCGUAUCCUCCUCUCUCUGACAAAAAUUG ............(((((.((....(((((....)))))...)))))))......((((((..((((((((((((.((..((((.((((((....))))))..(((((((...)))))))..)))))).))))))).)..).)))...))))))..((((((((...))))))))..(((((((((((((((......)))....))))))))))))........... (-80.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 795 140 93 148 99 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 732 140 92 222 176 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 731 130 84 179 126 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 665 134 94 209 165 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 637 125 84 53 12 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 551 140 94 169 126 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 467 131 94 193 156 aact:AA01502|5end_serine_acetyltransferase_1010014..1010244_POSITIVE 455 130 82 167 120 aact:AA02728|5end_conserved_hypothetical_protein_(possible_ATPase)_1910145..1910375_POSITIVE 447 115 94 231 210 aact:AA00096|5end_L-asparaginase_II_precursor_71280..71510_POSITIVE 417 129 84 104 49 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 416 138 94 146 98 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 385 220 178 164 127 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 385 138 95 218 164 aact:AA01815|5end_probable_sodium-dependent_transmembrane_transport_protein_1222541..1222771_POSITIVE 377 220 182 82 39 aact:AA00199|5end_hypothetical_protein_138575..138805_POSITIVE 374 139 91 169 125 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 374 148 101 217 164 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 374 127 85 183 134 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 373 125 85 190 122 aact:AA00132|5end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_94938..95168_POSITIVE 371 175 133 61 18 aact:AA02714|5end_paraquat-inducible_protein_PqiA_homolog_1895807..1896037_POSITIVE 367 120 71 89 24 aact:AA02089|5end_glycogen_operon_protein_1422050..1422280_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 795 140 93 96 47 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 781 138 95 46 1 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 732 140 92 92 46 aact:AA01934|3end_conserved_hypothetical_protein_1306993..1307143_POSITIVE 706 160 115 42 1 aact:AA00983|3end_aldo-keto_reductase_688492..688642_POSITIVE 617 125 94 54 23 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 551 140 94 138 95 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 467 131 94 139 102 aact:AA01501|3end_hypothetical_protein_1010040..1010190_POSITIVE 416 138 94 146 98 aact:AA00892|3end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_619959..620109_POSITIVE 385 220 178 74 37 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 385 157 111 53 1 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 377 220 182 70 27 aact:AA00198|3end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138643..138793_POSITIVE 372 170 130 143 94 aact:AA00199|3end_hypothetical_protein_138744..138894_POSITIVE 365 139 91 99 49 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 365 127 90 43 1 aact:AA00820|3end_succinyl-diaminopimelate_desuccinylase_564536..564686_POSITIVE 362 118 74 147 106 aact:AA01794|3end_glycine_cleavage_system_transcription_activator_1210690..1210840_POSITIVE 359 148 101 95 39 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 359 129 84 58 4 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 359 117 75 127 77 aact:AA00131|3end_transcriptional_regulator_(MerR_family)_94984..95134_POSITIVE 352 126 84 149 101 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 351 134 91 149 111 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE