Origin IGS: ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttggtcattttttgttaaatttatacagtagattgtatctttttaccccaaaagaatcatctgttgcaaagaaaattttttagaacggtaatttttttaccgaaaaagcgggcgatttttgttatagtaggtgcagtttttattcaattaaattaaggagaaaaaa Mask Tandem Repeat Region ================================================ ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Find is-nt database================================================ Query_seq: AA00190:AA00191|AA00190:AA00191:triosephosphate isomerase:conserved hypothetical protein (possible acetyltransferase, GNAT family):->->:134993..135158 166 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00190:AA00191|AA00190:AA00191:triosephosphate isomerase:conserved hypothetical protein (possible acetyltransferase, GNAT family):->->:134993..135158 166 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00190:AA00191|AA00190:AA00191:triosephosphate isomerase:conserved hypothetical protein (possible acetyltransferase, GNAT family):->->:134993..135158 166 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: - Start: 14 End: 148 ............. N L Q I F V A G V I V F I A R K K P L F I V T R F F K R Q L L H N K Q P L F I C D V T Y M .................. ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 64 HP_score: -6.6 Tail_Score: -3.33009 Start: 65 End: 81 Full_Region: AATCGCCCGCTTTTT CGGTAA AAAAA TTACCG TTCTAAAAAATTTTC ................................................................CGGTAAAAAAATTACCG..................................................................................... Find igs database================================================ Query_seq: AA00190:AA00191|AA00190:AA00191:triosephosphate isomerase:conserved hypothetical protein (possible acetyltransferase, GNAT family):->->:134993..135158 166 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Intra-Species Hit: Count: 1 Min: 1 Max: 166 Len: 166 Subject: aact_AA00190_AA00191|triosephosphate isomerase:conserved hypothetical protein (possible acetyltransferase, GNAT family)|POSITIVE:POSITIVE|[134993,135158]|166 HSP 1 e-value: 6.0E-77 bit: 287.0 Len: 166 Query Start:1 Query End:166 Subject Strand: POSITIVE Subject Start: 1 Subject End: 166 ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtnnnnnnnttaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa ttttttctccttaatttaattgaataaaaactgcacctactataacaaaaatcgcccgctttttcggtaaaaaaattaccgttctaaaaaattttctttgcaacagatgattcttttggggtaaaaagatacaatctactgtataaatttaacaaaaaatgaccaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUUCUCCUUAAUUUAAUUGAAUAAAAACUGCACCUACUAUAACAAAAAUCGCCCGCUUUUUCGGUAAAAAAAUUACCGUUCUAAAAAAUUUUCUUUGCAACAGAUGAUUCUUUUGGGGUAAAAAGAUACAAUCUACUGUAUAAAUUUAACAAAAAAUGACCAA ...................((((((............................((((.......((((((.....))))))...................(((.(((....))).)))))))......(((((......)))))..)))))).............. (-17.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGGUCAUUUUUUGUUAAAUUUAUACAGUAGAUUGUAUCUUUUUACCCCAAAAGAAUCAUCUGUUGCAAAGAAAAUUUUUUAGAACGGUAAUUUUUUUACCGAAAAAGCGGGCGAUUUUUGUUAUAGUAGGUGCAGUUUUUAUUCAAUUAAAUUAAGGAGAAAAAA .......((((((.((.((((((......(((((((((((...........(((((((..(((((..(((((....)))))....((((((.....))))))....)))))..)))))))........))))))))))).........)))))).)).)))))).. (-31.77) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 353 80 31 122 79 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 255 65 29 155 123 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 255 84 45 104 60 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 254 80 31 61 5 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 252 70 30 216 176 aact:AA01563|5end_transketolase_isozyme_1_1046969..1047199_POSITIVE 248 68 29 196 164 aact:AA00852|5end_hypothetical_protein_588538..588768_POSITIVE 247 70 30 54 9 aact:AA00614|5end_ribonuclease_HII_(RNase_HII)_419890..420120_POSITIVE 245 69 35 56 11 aact:AA02230|5end_sugar_kinase_1515984..1516214_POSITIVE 239 83 53 31 1 aact:AA02145|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468602..1468832_POSITIVE 239 145 105 224 190 aact:AA01507|5end_conserved_hypothetical_protein_1012433..1012663_POSITIVE 239 69 21 200 148 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 235 83 51 221 186 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 235 68 28 46 2 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 234 67 31 227 188 aact:AA01107|5end_adenylosuccinate_lyase_762310..762540_POSITIVE 232 65 23 108 65 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 231 70 21 77 27 aact:AA02589|5end_NAD(P)H_oxidoreductase_1800493..1800723_POSITIVE 231 80 35 136 99 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 230 59 30 225 195 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 229 67 29 138 83 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 229 84 45 101 52 aact:AA00142|5end_glycerate_kinase_101309..101539_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 272 84 46 139 102 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 255 65 29 129 97 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 252 70 30 138 98 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 247 70 30 58 13 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 247 69 30 138 78 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 232 65 23 95 52 aact:AA00906|3end_sigma-E_factor_regulatory_protein_626143..626293_POSITIVE 231 70 21 74 24 aact:AA02587|3end_conserved_hypothetical_protein_1800570..1800720_POSITIVE 231 80 35 121 84 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 230 61 31 122 88 aact:AA02190|3end_hydrogenase-2_component_protein_1493271..1493421_POSITIVE 223 58 11 143 96 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 223 80 32 117 74 aact:AA00309|3end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_210267..210417_POSITIVE 221 67 29 80 44 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 219 81 48 118 90 aact:AA01720|3end_conserved_hypothetical_protein_1163483..1163633_POSITIVE 218 143 113 48 3 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 217 72 40 45 9 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 211 42 9 132 98 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 209 84 45 145 102 aact:AA01991|3end_conserved_hypothetical_protein_(possible_nitroreductase)_1353003..1353153_POSITIVE 206 67 46 66 44 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 205 68 31 53 17 aact:AA01817|3end_hypothetical_protein_1224400..1224550_POSITIVE 204 80 31 124 78 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE