Origin IGS: attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaagtgcggtaggttttcgcgacgttttacaaattaatgcagtcgaaaagtggaaaatcattaaatccttaataagcaagtgaatgagaggagtagaat Mask Tandem Repeat Region ================================================ attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Find is-nt database================================================ Query_seq: AA00185:AA00186|AA00185:AA00186:3-dehydroquinate dehydratase II:o-succinylbenzoate-CoA synthase; OSB synthase:->->:131406..131506 101 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00185:AA00186|AA00185:AA00186:3-dehydroquinate dehydratase II:o-succinylbenzoate-CoA synthase; OSB synthase:->->:131406..131506 101 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00185:AA00186|AA00185:AA00186:3-dehydroquinate dehydratase II:o-succinylbenzoate-CoA synthase; OSB synthase:->->:131406..131506 101 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Predict ORF larger than 30AA ================================================ attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00185:AA00186|AA00185:AA00186:3-dehydroquinate dehydratase II:o-succinylbenzoate-CoA synthase; OSB synthase:->->:131406..131506 101 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA00185_AA00186|3-dehydroquinate dehydratase II:o-succinylbenzoate-CoA synthase; OSB synthase|POSITIVE:POSITIVE|[131406,131506]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta attctactcctctcattcacttgcttattaaggatttaatgattttccacttttcgactgcattaatttgtaaaacgtcgcgaaaacctaccgcactttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCUACUCCUCUCAUUCACUUGCUUAUUAAGGAUUUAAUGAUUUUCCACUUUUCGACUGCAUUAAUUUGUAAAACGUCGCGAAAACCUACCGCACUUUUA .....................(((.......(((...........)))..(((.((((((((......))))....)))).)))........)))...... (-10.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGUGCGGUAGGUUUUCGCGACGUUUUACAAAUUAAUGCAGUCGAAAAGUGGAAAAUCAUUAAAUCCUUAAUAAGCAAGUGAAUGAGAGGAGUAGAAU ......(((((.......)))))..(((((((...((((((...((........))....)))))).(((((......(....).....)))))))))))) (-14.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 489 100 52 122 77 aact:AA01181|5end_conserved_hypothetical_protein_808774..809004_POSITIVE 389 100 51 205 155 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 389 100 51 88 38 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 372 100 72 110 82 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 367 100 51 169 117 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 363 100 61 64 19 aact:AA00349|5end_hypothetical_protein_233274..233504_POSITIVE 359 99 53 126 78 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 359 99 58 147 106 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 348 100 66 143 107 aact:AA01416|5end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_952780..953010_POSITIVE 348 99 55 139 101 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 344 100 67 43 4 aact:AA02705|5end_hypothetical_protein_1886717..1886947_POSITIVE 341 100 53 140 95 aact:AA01694|5end_PTS_system,_IIA_component_1149929..1150159_POSITIVE 335 100 51 201 162 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 335 100 51 48 9 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 331 100 69 178 148 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 330 100 72 28 1 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 326 100 55 123 76 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 323 100 58 122 87 aact:AA00552|5end_periplasmic_nitrate_reductase_precursor_377331..377561_POSITIVE 321 100 54 227 184 aact:AA02776|5end_conserved_hypothetical_protein_1939693..1939923_POSITIVE 317 100 72 104 76 aact:AA01437|5end_phosphoglycerate_kinase_964125..964355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 489 100 52 69 24 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 389 100 51 79 29 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 363 100 61 46 1 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 359 99 53 49 1 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 359 99 58 89 48 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 348 100 66 143 107 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 348 99 55 136 98 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 344 100 67 41 2 aact:AA02704|3end_dihydrolipoamide_succinyltransferase_1886795..1886945_POSITIVE 341 100 53 60 15 aact:AA01692|3end_hexulose-6-phosphate_synthase_1149929..1150079_POSITIVE 338 100 56 82 43 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 335 100 51 85 46 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 332 100 67 121 87 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 331 100 69 47 17 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 325 99 68 32 1 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 323 100 58 114 79 aact:AA00551|3end_hypothetical_protein_377403..377553_POSITIVE 317 100 72 36 8 aact:AA01436|3end_fructose-bisphosphate_aldolase_class_II_964137..964287_POSITIVE 312 100 69 114 83 aact:AA01727|3end_conserved_hypothetical_protein_(possible_HipA_protein)_1168420..1168570_POSITIVE 311 100 69 141 109 aact:AA01389|3end_hypothetical_protein_937158..937308_POSITIVE 309 100 55 37 1 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE 307 100 52 130 91 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE