Origin IGS: atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagtgggattattaattaaatattagactattactttaatttctaaggagtttttttatgagtaattttaaacactcaatttcaaaattgagtcttattgtattaacatcattaaccgttacagcttgcggaagtggcggcggtggttccggtactccgacacctgtagttaatactggcgctgtattttcagtttcagaaaaaggtgatgtatcaaaaacttcaataaaaagcgttagatattgctggacataaaattaatctacttgaagattctgatagttatagttttataggcgagaacaatagcctttatcaatatggttatttatcttctaagacagaagatttccctaatgatcaagtattttacaatggagaaattgctaaaaatattcctaatgcgggtgaagtccgctattccggtagtgtagttataggtggtcagaagagtgatggcgcccctatcggagctagtgagcaattaacaaaaggtaaagcagaaattagagctgattttgggtcaaaaaaattagatggtcgatttatctttgggccaggaaccttagaacgtaaaaat Mask Tandem Repeat Region ================================================ atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Find is-nt database================================================ Query_seq: AA00180:AA00181|AA00180:AA00181:conserved hypothetical protein:hypothetical protein:->->:128129..128708 580 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00180:AA00181|AA00180:AA00181:conserved hypothetical protein:hypothetical protein:->->:128129..128708 580 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00180:AA00181|AA00180:AA00181:conserved hypothetical protein:hypothetical protein:->->:128129..128708 580 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 32 End: 133 ............................... M N R P S N F F D P K S A L I S A L P F V N C S L A P I G A P S L F ............................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 51 Strand: + Start: 97 End: 249 ................................................................................................ M L T S S D R G A I T L L T T Y N Y T T G I A D F T R I R N I F S N F S I V K Y L I I R E I F C L R R ........................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 354 End: 542 ................................................................................................................................................................................................................................................................................................................................................................. M E V F D T S P F S E T E N T A P V L T T G V G V P E P P P P L P Q A V T V N D V N T I R L N F E I E C L K L L I K K L ...................................... Protein_Len: 60 Strand: - Start: 329 End: 523 ........................................................................................................................................................................................................................................................................................................................................ V S K I S T K S V D G K E S V S F V A G T N V V P T P T G S G G G G S G C A T V T L S T L V I L S L K S I S H K F N S M ......................................................... Protein_Len: 42 Strand: - Start: 150 End: 275 ..................................................................................................................................................... V V P I A S K V R M L F I K L L K E M T F Y K I M L S I K Q R L L Y I V M N I F A M ................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 1 End: 339 Y R V E G A N P I N K A I E G N Y F V Q D N P F D E T K S S L Y G Y Q Y L S N N E G I F S Y S D S D E L L N I K H G A M ................................................................................................................................................................................................................................................. atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 472 End: 501 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTCAAAATTGAGTCTTATTGTATTAACAT............................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00180:AA00181|AA00180:AA00181:conserved hypothetical protein:hypothetical protein:->->:128129..128708 580 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Intra-Species Hit: Count: 1 Min: 1 Max: 580 Len: 580 Subject: aact_AA00180_AA00181|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[128129,128708]|580 HSP 1 e-value: 0.0 bit: 1066.0 Len: 580 Query Start:1 Query End:580 Subject Strand: POSITIVE Subject Start: 1 Subject End: 580 atttttacgttctaaggttcctggcccaaagataaatcgaccatctaannnnnnngacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcatnnnnnnnctccttagaaattaaagtaatagtctaatatttaattaataatcccacta atttttacgttctaaggttcctggcccaaagataaatcgaccatctaatttttttgacccaaaatcagctctaatttctgctttaccttttgttaattgctcactagctccgataggggcgccatcactcttctgaccacctataactacactaccggaatagcggacttcacccgcattaggaatatttttagcaatttctccattgtaaaatacttgatcattagggaaatcttctgtcttagaagataaataaccatattgataaaggctattgttctcgcctataaaactataactatcagaatcttcaagtagattaattttatgtccagcaatatctaacgctttttattgaagtttttgatacatcacctttttctgaaactgaaaatacagcgccagtattaactacaggtgtcggagtaccggaaccaccgccgccacttccgcaagctgtaacggttaatgatgttaatacaataagactcaattttgaaattgagtgtttaaaattactcataaaaaaactccttagaaattaaagtaatagtctaatatttaattaataatcccacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUUACGUUCUAAGGUUCCUGGCCCAAAGAUAAAUCGACCAUCUAAUUUUUUUGACCCAAAAUCAGCUCUAAUUUCUGCUUUACCUUUUGUUAAUUGCUCACUAGCUCCGAUAGGGGCGCCAUCACUCUUCUGACCACCUAUAACUACACUACCGGAAUAGCGGACUUCACCCGCAUUAGGAAUAUUUUUAGCAAUUUCUCCAUUGUAAAAUACUUGAUCAUUAGGGAAAUCUUCUGUCUUAGAAGAUAAAUAACCAUAUUGAUAAAGGCUAUUGUUCUCGCCUAUAAAACUAUAACUAUCAGAAUCUUCAAGUAGAUUAAUUUUAUGUCCAGCAAUAUCUAACGCUUUUUAUUGAAGUUUUUGAUACAUCACCUUUUUCUGAAACUGAAAAUACAGCGCCAGUAUUAACUACAGGUGUCGGAGUACCGGAACCACCGCCGCCACUUCCGCAAGCUGUAACGGUUAAUGAUGUUAAUACAAUAAGACUCAAUUUUGAAAUUGAGUGUUUAAAAUUACUCAUAAAAAAACUCCUUAGAAAUUAAAGUAAUAGUCUAAUAUUUAAUUAAUAAUCCCACUA ..(((((((....(((((...(((..((((((..................))))))..))).....(((..........)))..))))).....(((((((.....(((((....))))).((.......(((((....................)))))..((((.......))))....)).........))))))).......))))))).............((((.((((((((...)))))))).........(((((((..((((..........)))).....(((((.((((((((((.(((((((((((.....)))))....(((..........))).....)))))).)))))))..........(((((((..(((......))).....((((((((....(((..(((....)))..)))...((((..((...(....)..))..)))).......))))))))...((.((((((((....)))))))).))........................)))))))....))).)))))..........))))))).)))).... (-108.72) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUGGGAUUAUUAAUUAAAUAUUAGACUAUUACUUUAAUUUCUAAGGAGUUUUUUUAUGAGUAAUUUUAAACACUCAAUUUCAAAAUUGAGUCUUAUUGUAUUAACAUCAUUAACCGUUACAGCUUGCGGAAGUGGCGGCGGUGGUUCCGGUACUCCGACACCUGUAGUUAAUACUGGCGCUGUAUUUUCAGUUUCAGAAAAAGGUGAUGUAUCAAAAACUUCAAUAAAAAGCGUUAGAUAUUGCUGGACAUAAAAUUAAUCUACUUGAAGAUUCUGAUAGUUAUAGUUUUAUAGGCGAGAACAAUAGCCUUUAUCAAUAUGGUUAUUUAUCUUCUAAGACAGAAGAUUUCCCUAAUGAUCAAGUAUUUUACAAUGGAGAAAUUGCUAAAAAUAUUCCUAAUGCGGGUGAAGUCCGCUAUUCCGGUAGUGUAGUUAUAGGUGGUCAGAAGAGUGAUGGCGCCCCUAUCGGAGCUAGUGAGCAAUUAACAAAAGGUAAAGCAGAAAUUAGAGCUGAUUUUGGGUCAAAAAAAUUAGAUGGUCGAUUUAUCUUUGGGCCAGGAACCUUAGAACGUAAAAAU ((((((...((((.....)))).....)))))).((((..((((((((.........((((.((((.......((((((((....))))))))........))))...))))...((......(((..(...(((..((..((.(((.(((.(((((.(((((((((((......((((((((.((((...(((((..((.............))..)))))..))))...))))))))(((((((((((...............(((((((((...(((((..(((....((.((((..........)))).))....)))..))))).)))))).)))..((..(((((((((.((.....((((((((.((((......))))...))))))))....))..))).))))))..))..)))))))))))..)))))))).)))....))))).))).))).))..))..))).)..)))......(.(((((((((...((...((...((((((((........)))))))).)).))..))))))))).)....))..))))))))..))))... (-136.98) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 444 457 418 175 127 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 433 465 421 151 118 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 426 459 412 187 134 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 423 469 421 70 16 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 410 480 439 140 94 aact:AA02109|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436563..1436793_POSITIVE 393 460 419 65 19 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 392 457 412 177 122 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 389 457 416 220 163 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 382 457 418 60 2 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 379 467 430 187 130 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 376 465 428 231 177 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 365 460 413 99 53 aact:AA00936|5end_ribose_ABC_transporter,_permease_protein,_C-terminal_646289..646519_POSITIVE 364 127 88 188 146 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 364 127 88 50 8 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 364 452 413 68 28 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 363 466 425 213 160 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 362 466 423 150 116 aact:AA02422|5end_conserved_hypothetical_protein_1668014..1668244_POSITIVE 362 466 423 150 116 aact:AA02298|5end_conserved_hypothetical_protein_1570588..1570818_POSITIVE 362 150 101 118 73 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 362 466 423 150 116 aact:AA00622|5end_conserved_hypothetical_protein_429692..429922_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 433 465 421 148 115 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 423 469 421 74 20 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 410 480 439 140 94 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 393 460 419 65 19 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 379 467 430 129 72 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 366 445 401 144 109 aact:AA00741|3end_possible_2-oxoglutarate/malate_transporter_508076..508226_POSITIVE 365 460 413 58 12 aact:AA00935|3end_ribose_ABC_transporter,_permease_protein,_N-terminal_646328..646478_POSITIVE 350 446 416 142 111 aact:AA02470|3end_glucose_kinase_1707694..1707844_POSITIVE 343 458 419 47 11 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 341 460 413 64 25 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 341 459 411 140 100 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 339 469 422 60 7 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE 338 458 419 39 6 aact:AA01247|3end_hypothetical_protein_849944..850094_POSITIVE 333 140 97 52 3 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 331 460 419 114 53 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 328 449 401 92 28 aact:AA01987|3end_hypothetical_protein_1350620..1350770_POSITIVE 327 466 422 146 109 aact:AA01311|3end_transcriptional_regulator_889644..889794_POSITIVE 326 469 430 150 100 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 325 457 413 149 103 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 323 457 421 39 1 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE