Origin IGS: acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttacttcaaaatccatataaaatttatggataatgtcaagcctttcttatgattttttttcagaaatttgacgaagttaaaagaatgaaaaaaaattacagattgtctaaaaaatacacttttcaaacgccctaaaagtgcgt Mask Tandem Repeat Region ================================================ acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Find is-nt database================================================ Query_seq: AA00172:AA00173|AA00172:AA00173:conserved hypothetical protein (possible regulator protein / nitrogen fixation protein):hypothetical protein:->->:122965..123107 143 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00172:AA00173|AA00172:AA00173:conserved hypothetical protein (possible regulator protein / nitrogen fixation protein):hypothetical protein:->->:122965..123107 143 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00172:AA00173|AA00172:AA00173:conserved hypothetical protein (possible regulator protein / nitrogen fixation protein):hypothetical protein:->->:122965..123107 143 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 18 End: 140 ................. M K S V F F R Q S V I F F H S F N F V K F L K K N H K K G L T L S I N F I W I L K * ... acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00172:AA00173|AA00172:AA00173:conserved hypothetical protein (possible regulator protein / nitrogen fixation protein):hypothetical protein:->->:122965..123107 143 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Intra-Species Hit: Count: 1 Min: 1 Max: 143 Len: 143 Subject: aact_AA00172_AA00173|conserved hypothetical protein (possible regulator protein / nitrogen fixation protein):hypothetical protein|POSITIVE:POSITIVE|[122965,123107]|143 HSP 1 e-value: 3.0E-47 bit: 188.0 Len: 143 Query Start:1 Query End:143 Subject Strand: POSITIVE Subject Start: 1 Subject End: 143 acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaannnnnnnncattcttttaacttcgtcaaatttctgnnnnnnnntcataagaaaggcttgacattatccataaattttatatggattttgaagtaa acgcacttttagggcgtttgaaaagtgtattttttagacaatctgtaattttttttcattcttttaacttcgtcaaatttctgaaaaaaaatcataagaaaggcttgacattatccataaattttatatggattttgaagtaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACGCACUUUUAGGGCGUUUGAAAAGUGUAUUUUUUAGACAAUCUGUAAUUUUUUUUCAUUCUUUUAACUUCGUCAAAUUUCUGAAAAAAAAUCAUAAGAAAGGCUUGACAUUAUCCAUAAAUUUUAUAUGGAUUUUGAAGUAA ..((.(((((.....((((((((((....))))))))))....(((.(((((((((((....(((.((...)).)))....))))))))))).))).)))))))....((..(((((((.......)))))))..))...... (-26.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACUUCAAAAUCCAUAUAAAAUUUAUGGAUAAUGUCAAGCCUUUCUUAUGAUUUUUUUUCAGAAAUUUGACGAAGUUAAAAGAAUGAAAAAAAAUUACAGAUUGUCUAAAAAAUACACUUUUCAAACGCCCUAAAAGUGCGU ..........(((((((.......))))))).......((.(..(((..(((((((((((((....((((((...))))))....))))))))))))).)))..).))........(((((((..........)))))))... (-25.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 275 109 61 189 150 aact:AA02491|5end_topoisomerase_IV,_subunit_A_1726827..1727057_POSITIVE 275 109 61 195 156 aact:AA00573|5end_chloramphenicol-sensitive_protein_RarD_391965..392195_POSITIVE 262 60 11 92 36 aact:AA00631|5end_hypothetical_protein_433678..433908_POSITIVE 254 42 2 216 164 aact:AA00185|5end_3-dehydroquinate_dehydratase_II_130792..131022_POSITIVE 254 42 2 106 54 aact:AA00184|5end_hypothetical_protein_130682..130912_POSITIVE 242 132 92 107 61 aact:AA02755|5end_hypothetical_protein_1929303..1929533_POSITIVE 236 45 2 139 87 aact:AA00235|5end_hypothetical_protein_168596..168826_POSITIVE 225 118 92 169 143 aact:AA01294|5end_DNA_adenine_methylase_876415..876645_POSITIVE 224 40 2 99 65 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 224 46 2 206 161 aact:AA00543|5end_ADP-heptose--LPS_heptosyltransferase_II_371287..371517_POSITIVE 224 104 61 208 167 aact:AA00241|5end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_171546..171776_POSITIVE 224 104 61 52 11 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 222 137 95 124 82 aact:AA02976|5end_UDP-2,3-diacylglucosamine_hydrolase_2080229..2080459_POSITIVE 222 60 11 207 151 aact:AA01549|5end_hypothetical_protein_1037444..1037674_POSITIVE 222 48 3 87 43 aact:AA01442|5end_phosphatidylserine_synthetase_967749..967979_POSITIVE 221 29 2 209 176 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 221 29 2 67 34 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 221 30 2 128 101 aact:AA01220|5end_competence_protein_F;_amidophosphoribosyltransferase_832775..833005_POSITIVE 220 29 2 121 96 aact:AA01781|5end_molybdenum_ABC_transporter,_periplasmic_substrate-binding_1201891..1202121_POSITIVE 220 20 2 44 28 aact:AA01551|5end_hypothetical_protein_1038190..1038420_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 254 42 2 89 37 aact:AA00183|3end_acetyl-CoA_carboxylase,_biotin_carboxyl_carrier_protein_130745..130895_POSITIVE 242 132 92 82 36 aact:AA02754|3end_conserved_hypothetical_protein_1929358..1929508_POSITIVE 236 45 2 104 52 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 225 118 92 57 31 aact:AA01292|3end_single-stranded_DNA_binding_protein_876383..876533_POSITIVE 224 40 2 99 65 aact:AA01187|3end_conserved_hypothetical_protein_813992..814142_POSITIVE 222 137 95 128 86 aact:AA02975|3end_conserved_hypothetical_protein_2080313..2080463_POSITIVE 221 29 2 95 62 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 221 30 2 128 101 aact:AA01217|3end_hypothetical_protein_832855..833005_POSITIVE 220 29 2 84 59 aact:AA01780|3end_ABC-related_molybdenum_transport_system_permease_protein_1201934..1202084_POSITIVE 220 20 2 148 132 aact:AA01551|3end_hypothetical_protein_1038374..1038524_POSITIVE 220 20 2 66 50 aact:AA01550|3end_hypothetical_protein_1038292..1038442_POSITIVE 218 32 1 43 11 aact:AA02490|3end_hypothetical_protein_1726896..1727046_POSITIVE 218 46 2 114 68 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 218 132 102 134 100 aact:AA01072|3end_GTP_pyrophosphokinase_(ppGpp_synthetase_I)_/_ATP:GTP3'-pyrophosphotransferase_745242..745392_POSITIVE 216 44 2 143 105 aact:AA00841|3end_UDP-MurNAc-tripeptide_synthetase_582861..583011_POSITIVE 213 39 2 99 68 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 210 47 13 71 37 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 210 40 2 58 17 aact:AA01581|3end_possible_GTP-binding_protein_1065518..1065668_POSITIVE 210 38 2 79 45 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 209 35 2 75 45 aact:AA00116|3end_conserved_hypothetical_protein_88207..88357_POSITIVE