Origin IGS: atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagtttttaactcattttttaccatcgcccttgtcctgttgacaagggttttttattgtgaacccgtgtatcgagtatttattcctagtctcctaaagccatttacatcgctcataactgcggactaaacggggtagcgcattgaacgtagcttgat Mask Tandem Repeat Region ================================================ atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Find is-nt database================================================ Query_seq: AA00170:AA00171|AA00170:AA00171:hypothetical protein:DNA-binding protein:->->:121468..121624 157 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00170:AA00171|AA00170:AA00171:hypothetical protein:DNA-binding protein:->->:121468..121624 157 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00170:AA00171|AA00170:AA00171:hypothetical protein:DNA-binding protein:->->:121468..121624 157 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: - Start: 33 End: 134 ................................ D A T I L S T F P K L L S P I F V R Y V P E C Y F V R T L L V L A M ....................... atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00170:AA00171|AA00170:AA00171:hypothetical protein:DNA-binding protein:->->:121468..121624 157 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Intra-Species Hit: Count: 1 Min: 1 Max: 157 Len: 157 Subject: aact_AA00170_AA00171|hypothetical protein:DNA-binding protein|POSITIVE:POSITIVE|[121468,121624]|157 HSP 1 e-value: 4.0E-84 bit: 311.0 Len: 157 Query Start:1 Query End:157 Subject Strand: POSITIVE Subject Start: 1 Subject End: 157 atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta atcaagctacgttcaatgcgctaccccgtttagtccgcagttatgagcgatgtaaatggctttaggagactaggaataaatactcgatacacgggttcacaataaaaaacccttgtcaacaggacaagggcgatggtaaaaaatgagttaaaaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCAAGCUACGUUCAAUGCGCUACCCCGUUUAGUCCGCAGUUAUGAGCGAUGUAAAUGGCUUUAGGAGACUAGGAAUAAAUACUCGAUACACGGGUUCACAAUAAAAAACCCUUGUCAACAGGACAAGGGCGAUGGUAAAAAAUGAGUUAAAAACUA ....((((.((((...(((.....(((((.((...((.(((...((((.((....)).))))(((....))).........))))).)).)))))..............((((((((.....)))))))).....)))...))))))))........ (-36.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUUUUAACUCAUUUUUUACCAUCGCCCUUGUCCUGUUGACAAGGGUUUUUUAUUGUGAACCCGUGUAUCGAGUAUUUAUUCCUAGUCUCCUAAAGCCAUUUACAUCGCUCAUAACUGCGGACUAAACGGGGUAGCGCAUUGAACGUAGCUUGAU ...........(((....((((....(((((((((.....)))))))))..((((.((((..(((((.....((((....)))).(((((...((((....))))...(((........)))))))).)))))....)))).)))).))))..))). (-35.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 372 47 10 222 186 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 360 60 16 224 180 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 344 67 24 90 42 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 341 49 6 46 1 aact:AA00172|5end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_121778..122008_POSITIVE 319 47 4 91 30 aact:AA02397|5end_RNA_polymerase_sigma_factor_sigma-70_1647095..1647325_POSITIVE 315 60 17 49 3 aact:AA01744|5end_peptide_chain_release_factor_RF-2_1179373..1179603_POSITIVE 307 40 24 32 16 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 303 60 18 130 83 aact:AA02727|5end_hypothetical_protein_1909735..1909965_POSITIVE 303 60 14 203 137 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 302 51 11 83 37 aact:AA02964|5end_opacity_associated_protein_A_2072738..2072968_POSITIVE 301 60 12 222 171 aact:AA02357|5end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1614668..1614898_POSITIVE 300 50 10 189 148 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 299 49 5 223 180 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 298 50 9 54 11 aact:AA00384|5end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_260108..260338_POSITIVE 293 50 7 100 47 aact:AA02544|5end_ATP-dependent_RNA_helicase_1773317..1773547_POSITIVE 293 51 17 178 138 aact:AA01203|5end_diaminopimelate_epimerase_824140..824370_POSITIVE 291 50 2 83 15 aact:AA02321|5end_conserved_hypothetical_protein_1585163..1585393_POSITIVE 289 60 17 60 7 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 286 60 18 179 142 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 286 40 10 73 40 aact:AA00523|5end_conserved_hypothetical_protein_358472..358702_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 60 12 130 79 aact:AA02356|3end_hypothetical_protein_1614656..1614806_POSITIVE 301 49 10 60 15 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 298 50 9 48 5 aact:AA00383|3end_chloride_channel_protein_260182..260332_POSITIVE 293 50 7 137 84 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 292 50 5 76 24 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 291 50 2 74 6 aact:AA02320|3end_ABC_transporter_ATP-binding_protein_1585234..1585384_POSITIVE 284 60 11 110 44 aact:AA02735|3end_DNA_repair_protein_1917424..1917574_POSITIVE 282 70 27 132 97 aact:AA00273|3end_fatty_acid/phospholipid_synthesis_protein_192499..192649_POSITIVE 281 60 18 105 52 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 281 48 9 46 12 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 279 60 16 54 3 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 278 75 33 67 27 aact:AA00451|3end_hypothetical_protein_315348..315498_POSITIVE 274 69 24 107 67 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 273 50 5 149 103 aact:AA00931|3end_elongation_factor_Tu_(tufA)_643181..643331_POSITIVE 271 49 2 150 105 aact:AA02541|3end_conserved_hypothetical_protein_1772644..1772794_POSITIVE 271 50 5 41 4 aact:AA00983|3end_aldo-keto_reductase_688492..688642_POSITIVE 270 60 13 95 54 aact:AA02645|3end_dTDP-4-rhamnose_reductase_1842419..1842569_POSITIVE 268 46 1 122 78 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 268 41 4 83 50 aact:AA02005|3end_vancomycin_sensitivity;_SanA_protein_1364020..1364170_POSITIVE 267 50 7 136 83 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE