Origin IGS: taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacctaacaaacagcattcggcaaacccgaacggatccgtccttggtatcagtcctttccaacaccaatggccgcatcgacatcatgatgccacgtacgtatacagaaattagaccacagtcatttctaactacatttttcacatacaaaagtactgatagttttcgctataactgtttttctttagatttaggcgggttattccaagccgataacttctcggccgttgtgggaaggttcttcaattttacttttttcacattttgcgtgtagcctatgataggtttgttattgaataaataagcttttttgtggcatcacaataaaacgtactcctgcttcctgaagtacgtttggtttttccggaatatta Mask Tandem Repeat Region ================================================ taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Find is-nt database================================================ Query_seq: AA00169:AA00170|AA00169:AA00170:conserved hypothetical protein:hypothetical protein:->->:121004..121377 374 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00169:AA00170|AA00169:AA00170:conserved hypothetical protein:hypothetical protein:->->:121004..121377 374 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00169:AA00170|AA00169:AA00170:conserved hypothetical protein:hypothetical protein:->->:121004..121377 374 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 93 End: 203 ............................................................................................ M I G Y T Q N V K K V K L K N L P T T A E K L S A W N N P P K S K E K Q L ........................................................................................................................................................................... Protein_Len: 50 Strand: + Start: 225 End: 374 ................................................................................................................................................................................................................................ M Y V K N V V R N D C G L I S V Y V R G I M M S M R P L V L E R T D T K D G S V R V C R M L F V R L Protein_Len: 60 Strand: - Start: 104 End: 358 ....................................................................................................... G L D L S F C N Y R F S D T S K Y T F F T T L F S Q P R I E T Y T R P M M I D I R G N T N S L V S V L S P D T R T Q R M ................ taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00169:AA00170|AA00169:AA00170:conserved hypothetical protein:hypothetical protein:->->:121004..121377 374 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Intra-Species Hit: Count: 1 Min: 1 Max: 374 Len: 374 Subject: aact_AA00169_AA00170|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[121004,121377]|374 HSP 1 e-value: 0.0 bit: 741.0 Len: 374 Query Start:1 Query End:374 Subject Strand: POSITIVE Subject Start: 1 Subject End: 374 taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta taatattccggaaaaaccaaacgtacttcaggaagcaggagtacgttttattgtgatgccacaaaaaagcttatttattcaataacaaacctatcataggctacacgcaaaatgtgaaaaaagtaaaattgaagaaccttcccacaacggccgagaagttatcggcttggaataacccgcctaaatctaaagaaaaacagttatagcgaaaactatcagtacttttgtatgtgaaaaatgtagttagaaatgactgtggtctaatttctgtatacgtacgtggcatcatgatgtcgatgcggccattggtgttggaaaggactgataccaaggacggatccgttcgggtttgccgaatgctgtttgttaggtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUUCCGGAAAAACCAAACGUACUUCAGGAAGCAGGAGUACGUUUUAUUGUGAUGCCACAAAAAAGCUUAUUUAUUCAAUAACAAACCUAUCAUAGGCUACACGCAAAAUGUGAAAAAAGUAAAAUUGAAGAACCUUCCCACAACGGCCGAGAAGUUAUCGGCUUGGAAUAACCCGCCUAAAUCUAAAGAAAAACAGUUAUAGCGAAAACUAUCAGUACUUUUGUAUGUGAAAAAUGUAGUUAGAAAUGACUGUGGUCUAAUUUCUGUAUACGUACGUGGCAUCAUGAUGUCGAUGCGGCCAUUGGUGUUGGAAAGGACUGAUACCAAGGACGGAUCCGUUCGGGUUUGCCGAAUGCUGUUUGUUAGGUUA ..............((((.((((((((((.(....).)))))))))).(((..(((((((((......(((..........(((((..........(((((..((((.....)))).........................(((....((((((.......)))))))))........)))))..((....))......))))))))........(((((((....)))).)))...(((((..((((((((((....)))..)))))))..)))))..)))))))))..)))..(((.((.((.(((((((..(......)..))))))))).)).)))(((((((.....)))))))..........)))). (-99.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCUAACAAACAGCAUUCGGCAAACCCGAACGGAUCCGUCCUUGGUAUCAGUCCUUUCCAACACCAAUGGCCGCAUCGACAUCAUGAUGCCACGUACGUAUACAGAAAUUAGACCACAGUCAUUUCUAACUACAUUUUUCACAUACAAAAGUACUGAUAGUUUUCGCUAUAACUGUUUUUCUUUAGAUUUAGGCGGGUUAUUCCAAGCCGAUAACUUCUCGGCCGUUGUGGGAAGGUUCUUCAAUUUUACUUUUUUCACAUUUUGCGUGUAGCCUAUGAUAGGUUUGUUAUUGAAUAAAUAAGCUUUUUUGUGGCAUCACAAUAAAACGUACUCCUGCUUCCUGAAGUACGUUUGGUUUUUCCGGAAUAUUA .................(((((.(((.(((((((((((((....))).)))...((((((((....(((((((((...............(((......(((...((((((..(((....)))))))))...)))..............((((....(((((((........)))))))...)))).......))).((((......))))..........)))))))))))))))))..(((((.......................(((..(((...((.(((((((((........))))))))).))...)))..)))........(((....)))....)))))..))))))).))).)))))...... (-85.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 349 301 209 169 aact:AA00918|5end_hypothetical_protein_630044..630274_POSITIVE 376 364 322 58 14 aact:AA02697|5end_hydrogenase_3_maturation_protease_1879870..1880100_POSITIVE 369 337 291 183 135 aact:AA00299|5end_hypothetical_protein_204256..204486_POSITIVE 366 350 301 185 130 aact:AA01080|5end_hypothetical_protein_747679..747909_POSITIVE 362 360 311 85 31 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 360 317 278 117 72 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 357 316 274 75 34 aact:AA00921|5end_conserved_hypothetical_protein_633332..633562_POSITIVE 351 320 278 205 162 aact:AA02605|5end_hypothetical_protein_1816555..1816785_POSITIVE 349 340 291 68 9 aact:AA01968|5end_hypothetical_protein_1331738..1331968_POSITIVE 346 360 319 214 155 aact:AA00244|5end_conserved_hypothetical_protein_(possible_deoxyribonuclease,_possible_hydrolase)_172043..172273_POSITIVE 341 319 279 202 154 aact:AA02193|5end_oxaloacetate_decarboxylase_gamma_chain_1494219..1494449_POSITIVE 340 320 273 227 182 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 334 350 301 85 44 aact:AA00272|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_190406..190636_POSITIVE 333 337 293 208 154 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 330 364 323 51 7 aact:AA01776|5end_conserved_hypothetical_protein_1196074..1196304_POSITIVE 329 314 275 71 29 aact:AA01124.1|5end_biopolymer_transport_protein_D_772229..772459_POSITIVE 327 340 295 80 25 aact:AA02713|5end_possible_prop_effector_homolog_1894984..1895214_POSITIVE 325 340 292 60 6 aact:AA02772|5end_hypothetical_protein_1936244..1936474_POSITIVE 325 322 284 231 188 aact:AA01547|5end_deoxyribose-phosphate_aldolase_1036271..1036501_POSITIVE 324 348 303 175 122 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 411 325 282 139 81 aact:AA02350|3end_possible_lipoprotein_1609179..1609329_POSITIVE 376 364 322 65 21 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 364 348 301 54 5 aact:AA02723|3end_hypothetical_protein_1906453..1906603_POSITIVE 334 350 301 68 27 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 330 315 278 146 109 aact:AA02604|3end_thioredoxin_reductase_1816582..1816732_POSITIVE 330 364 323 79 35 aact:AA01775|3end_hypothetical_protein_1196182..1196332_POSITIVE 329 314 275 64 22 aact:AA01124|3end_biopolymer_transport_protein_772302..772452_POSITIVE 329 314 278 150 108 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 322 316 275 129 86 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 309 328 289 46 8 aact:AA02536|3end_conserved_hypothetical_protein_1765263..1765413_POSITIVE 309 338 292 86 33 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 309 180 132 66 26 aact:AA01987|3end_hypothetical_protein_1350620..1350770_POSITIVE 309 312 275 38 6 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 309 349 304 149 103 aact:AA00799|3end_iron(III)_dicitrate_transport_ATP-binding_protein_548502..548652_POSITIVE 307 320 275 49 15 aact:AA00240|3end_hypothetical_protein_171592..171742_POSITIVE 306 182 141 146 99 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 306 325 281 124 77 aact:AA00653|3end_hypothetical_protein_447860..448010_POSITIVE 306 316 275 150 109 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 303 358 317 83 39 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 303 344 301 126 78 aact:AA00801|3end_lysine--tRNA_ligase_549539..549689_POSITIVE