Origin IGS: aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatgttctcctttcatttgttatttttgatgtgtttatttaatacgaggaaaattttatcaccggtgtgtcaaggcgggtagtgataaatatgatttgattaaaaaaattacccaagggggcaaaagcctagtataatgtaccggttttattttctgaattagcgtgcggctaacaaaagtaattttctgcgtggtagccaatgaaagtgcggtcggttttaagattatttttgctagtcgcaaattggaaaataaaacatcgttattatttatttgcaaaaaggaaaacatt Mask Tandem Repeat Region ================================================ aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Find is-nt database================================================ Query_seq: AA00151:AA00153|AA00151:AA00153:polynucleotide phosphorylase:conserved hypothetical protein:->->:111132..111424 293 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00151:AA00153|AA00151:AA00153:polynucleotide phosphorylase:conserved hypothetical protein:->->:111132..111424 293 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00151:AA00153|AA00151:AA00153:polynucleotide phosphorylase:conserved hypothetical protein:->->:111132..111424 293 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 2 End: 232 . M F S F L Q I N N N D V L F S N L R L A K I I L K P T A L S L A T T Q K I T F V S R T L I Q K I K P V H Y T R L L P P W ............................................................. Protein_Len: 31 Strand: - Start: 160 End: 252 ............................................................................................................................................................... V L S K G G Q T I K K I L D Y K D S G A K V C R H Y F K G R M ......................................... Protein_Len: 30 Strand: - Start: 3 End: 92 .. T K R K A F L Y Y R H K I K W N A V L L F L R L V S R V K M ......................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 1 End: 135 I N E K K C I F L L S T K N E L K R S A F I I K F G V A S E N A V V C F I V K T L R V S M .............................................................................................................................................................. aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 84 HP_score: -5.4 Tail_Score: -5.52986 Start: 170 End: 185 Full_Region: CATTATACTAGGCTT TTGCCC CCTT GGGTAA TTTTTTTAATCAAAT .........................................................................................................................................................................TTGCCCCCTTGGGTAA............................................................................................................ Find igs database================================================ Query_seq: AA00151:AA00153|AA00151:AA00153:polynucleotide phosphorylase:conserved hypothetical protein:->->:111132..111424 293 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Intra-Species Hit: Count: 1 Min: 1 Max: 293 Len: 293 Subject: aact_AA00151_AA00153|polynucleotide phosphorylase:conserved hypothetical protein|POSITIVE:POSITIVE|[111132,111424]|293 HSP 1 e-value: 1.0E-152 bit: 539.0 Len: 293 Query Start:1 Query End:293 Subject Strand: POSITIVE Subject Start: 1 Subject End: 293 aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaannnnnnnaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc aatgttttcctttttgcaaataaataataacgatgttttattttccaatttgcgactagcaaaaataatcttaaaaccgaccgcactttcattggctaccacgcagaaaattacttttgttagccgcacgctaattcagaaaataaaaccggtacattatactaggcttttgcccccttgggtaatttttttaatcaaatcatatttatcactacccgccttgacacaccggtgataaaattttcctcgtattaaataaacacatcaaaaataacaaatgaaaggagaacatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUUUUCCUUUUUGCAAAUAAAUAAUAACGAUGUUUUAUUUUCCAAUUUGCGACUAGCAAAAAUAAUCUUAAAACCGACCGCACUUUCAUUGGCUACCACGCAGAAAAUUACUUUUGUUAGCCGCACGCUAAUUCAGAAAAUAAAACCGGUACAUUAUACUAGGCUUUUGCCCCCUUGGGUAAUUUUUUUAAUCAAAUCAUAUUUAUCACUACCCGCCUUGACACACCGGUGAUAAAAUUUUCCUCGUAUUAAAUAAACACAUCAAAAAUAACAAAUGAAAGGAGAACAUC .(((((((((((((.................((((((((((((.......(((((.((((..................((........))...(((((....((((((......)))))))))))....)))).....((((((...((((((...........(((....))).....(((((............................)))))..........))))))......)))))).)))))..))))))).))))).............))))))))))))). (-57.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUGUUCUCCUUUCAUUUGUUAUUUUUGAUGUGUUUAUUUAAUACGAGGAAAAUUUUAUCACCGGUGUGUCAAGGCGGGUAGUGAUAAAUAUGAUUUGAUUAAAAAAAUUACCCAAGGGGGCAAAAGCCUAGUAUAAUGUACCGGUUUUAUUUUCUGAAUUAGCGUGCGGCUAACAAAAGUAAUUUUCUGCGUGGUAGCCAAUGAAAGUGCGGUCGGUUUUAAGAUUAUUUUUGCUAGUCGCAAAUUGGAAAAUAAAACAUCGUUAUUAUUUAUUUGCAAAAAGGAAAACAUU ((((((.((((((.....((((....))))(((..((..((((((((.............(((((((..(......(((((((........................)))))))....((((....))))......)..)))))))(((((((((..(.......((((((((.(((((((((((((..((.(((..((..((....))))..)))))...))))))))))))).))))))))..)..)))))))))...))).)))))..))..)))..)))))).)))))) (-75.96) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 100 53 58 7 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 357 97 59 200 159 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 357 97 59 49 8 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 353 103 61 190 146 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 349 105 61 172 122 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 346 114 75 61 25 aact:AA01140|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_781610..781840_POSITIVE 325 89 49 181 145 aact:AA01251|5end_hypothetical_protein_851825..852055_POSITIVE 320 130 85 70 15 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 318 139 92 215 160 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 317 102 61 91 50 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 314 92 54 134 89 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 313 98 70 208 177 aact:AA01462|5end_xylose_operon_regulatory_protein_981530..981760_POSITIVE 313 98 70 90 59 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 309 104 61 181 137 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 306 100 52 153 103 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 306 114 78 61 21 aact:AA01551|5end_hypothetical_protein_1038190..1038420_POSITIVE 306 104 72 56 23 aact:AA00107|5end_histidyl-tRNA_synthetase_76699..76929_POSITIVE 305 97 56 78 33 aact:AA01745|5end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180394..1180624_POSITIVE 304 97 59 152 101 aact:AA01722|5end_conserved_hypothetical_protein_1163434..1163664_POSITIVE 297 90 53 78 38 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 104 67 137 94 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 351 89 41 89 45 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 346 114 75 56 20 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 334 132 92 54 1 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 333 233 194 123 90 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 325 89 49 56 20 aact:AA01250|3end_nadAB_transcriptional_regulator_851780..851930_POSITIVE 324 100 53 139 94 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE 321 120 73 112 60 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 320 130 85 87 32 aact:AA01773|3end_hypothetical_protein_1195112..1195262_POSITIVE 317 102 61 59 18 aact:AA01985|3end_pyruvate_dehydrogenase,_E1_component_1348801..1348951_POSITIVE 314 92 54 111 66 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 313 98 70 90 59 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 313 89 44 145 98 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 311 137 92 126 84 aact:AA01317|3end_probable_DNA_repair_protein_891739..891889_POSITIVE 306 114 78 83 43 aact:AA01550|3end_hypothetical_protein_1038292..1038442_POSITIVE 304 97 59 121 70 aact:AA01720|3end_conserved_hypothetical_protein_1163483..1163633_POSITIVE 295 249 215 79 47 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 294 88 42 151 113 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 293 118 73 96 60 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 293 88 44 55 4 aact:AA00702|3end_conserved_hypothetical_protein_483848..483998_POSITIVE