Origin IGS: taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaagcttaaacttcagtcttttcaaataccctaacgaatttactttctactctacctgtttggagtactatgcagacttccaatgactaccgttcatcttcattgcaatacttaacagatgtggcttatgttagcagagttttaattgttgtcaataagaattattatcatttacaaaaaatatttta Mask Tandem Repeat Region ================================================ taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Find is-nt database================================================ Query_seq: AA00139:AA00140|AA00139:AA00140:antibiotic maturation factor:hypothetical protein:->->:100597..100785 189 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00139:AA00140|AA00139:AA00140:antibiotic maturation factor:hypothetical protein:->->:100597..100785 189 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00139:AA00140|AA00139:AA00140:antibiotic maturation factor:hypothetical protein:->->:100597..100785 189 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 3 End: 119 .. F I K Q L H Y Y N K N V V I L V R S V Y A V D T L Y Q L S S S R Y D N S T Q M ...................................................................... taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00139:AA00140|AA00139:AA00140:antibiotic maturation factor:hypothetical protein:->->:100597..100785 189 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Intra-Species Hit: Count: 1 Min: 1 Max: 189 Len: 189 Subject: aact_AA00139_AA00140|antibiotic maturation factor:hypothetical protein|POSITIVE:POSITIVE|[100597,100785]|189 HSP 1 e-value: 1.0E-103 bit: 375.0 Len: 189 Query Start:1 Query End:189 Subject Strand: POSITIVE Subject Start: 1 Subject End: 189 taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca taaaatattttttgtaaatgataataattcttattgacaacaattaaaactctgctaacataagccacatctgttaagtattgcaatgaagatgaacggtagtcattggaagtctgcatagtactccaaacaggtagagtagaaagtaaattcgttagggtatttgaaaagactgaagtttaagcttca Inter-species Hit: Count: 1 Min: 15 Max: 162 Len: 148 Subject: NC_006300_MS0682_MS0683|TldD protein:hypoxanthine-guanine phosphoribosyltransferase|POSITIVE:POSITIVE|[635491, 635740]|250 HSP 1 e-value: 3.0E-11 bit: 69.9 Len: 39 Query Start:124 Query End:162 Subject Strand: POSITIVE Subject Start: 125 Subject End: 163 ...........................................................................................................................ctccaaacaggtagagtagaaagtaaattcgttagggta........................... ...........................................................................................................................ctccgaacaggtagagtagaaagtaaattcgttagggta........................... HSP 2 e-value: 7.0E-6 bit: 52.0 Len: 26 Query Start:15 Query End:40 Subject Strand: POSITIVE Subject Start: 12 Subject End: 37 ..............taaatgataataattcttattgacaa..................................................................................................................................................... ..............taaatgataataattcttattgacaa..................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUGAUAAUAAUUCUUAUUGACAACAAUUAAAACUCUGCUAACAUAAGCCACAUCUGUUAAGUAUUGCAAUGAAGAUGAACGGUAGUCAUUGGAAGUCUGCAUAGUACUCCAAACAGGUAGAGUAGAAAGUAAAUUCGUUAGGGUA ..............(((((.(((...........((((((((.......(((.(((((.....(.....).....)))))...))).....(((((.((((....)).)))))))...))))))))...........))).))))).. (-24.35) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACCCUAACGAAUUUACUUUCUACUCUACCUGUUUGGAGUACUAUGCAGACUUCCAAUGACUACCGUUCAUCUUCAUUGCAAUACUUAACAGAUGUGGCUUAUGUUAGCAGAGUUUUAAUUGUUGUCAAUAAGAAUUAUUAUCAUUUA .........((..........(((((((......))))))).............................((((.(((((((((.((((..(((...(((......)))...))))))).))))).)))))))).......))..... (-19.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 104 68 140 103 aact:AA02360|5end_threonine_synthase_1618596..1618826_POSITIVE 260 91 52 90 43 aact:AA01684|5end_ABC_transporter,_ATP-binding_protein;_possible_ATP-binding_component_of_sulfate_permease_A_protein_1136302..1136532_POSITIVE 258 98 53 88 24 aact:AA02001|5end_conserved_hypothetical_protein_1359842..1360072_POSITIVE 249 86 46 188 141 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 249 112 72 198 158 aact:AA00998|5end_small_protein_A_homolog_698525..698755_POSITIVE 248 124 82 85 39 aact:AA00431|5end_conserved_hypothetical_protein_293054..293284_POSITIVE 246 86 50 40 9 aact:AA02327|5end_possible_ABC-type_transport_system_1588655..1588885_POSITIVE 245 107 66 94 55 aact:AA00422|5end_LapB_membrane_protein_285528..285758_POSITIVE 240 120 72 96 38 aact:AA01327|5end_possible_LOS_biosynthesis_enzyme_(D-glycero-D-manno-heptosyl_transferase)_895568..895798_POSITIVE 238 79 33 55 12 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 238 120 71 104 59 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 235 127 98 109 79 aact:AA00175|5end_cell_filamentation_protein_123922..124152_POSITIVE 234 100 57 173 131 aact:AA02686|5end_hydrogenase_4_component_C_1871546..1871776_POSITIVE 232 140 91 66 8 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 231 140 94 178 121 aact:AA02602|5end_conserved_hypothetical_protein_1813407..1813637_POSITIVE 230 78 39 75 14 aact:AA00870|5end_rough_colony_protein_C_601735..601965_POSITIVE 229 122 84 188 147 aact:AA02702|5end_succinyl-CoA_synthetase,_beta_chain_1884139..1884369_POSITIVE 229 87 42 82 26 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 225 102 68 54 15 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 223 126 83 131 79 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 323 104 68 81 44 aact:AA02359|3end_hypothetical_protein_1618617..1618767_POSITIVE 260 91 52 150 103 aact:AA01682|3end_molybdate_ABC_transporter_protein_1136442..1136592_POSITIVE 259 109 71 132 81 aact:AA00661|3end_outer_membrane_protein_454531..454681_POSITIVE 248 124 82 141 95 aact:AA00430|3end_hypothetical_protein_293190..293340_POSITIVE 240 120 72 87 29 aact:AA01325|3end_formamidopyrimidine-DNA_glycosylase_895639..895789_POSITIVE 239 90 49 151 94 aact:AA01567|3end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1055215..1055365_POSITIVE 235 127 98 140 110 aact:AA00174|3end_ribosomal_protein_L11_methyltransferase_124033..124183_POSITIVE 233 120 72 83 25 aact:AA02417|3end_GTP-binding_protein_1660978..1661128_POSITIVE 230 114 83 151 125 aact:AA02421|3end_conserved_hypothetical_protein_1667300..1667450_POSITIVE 223 108 67 85 41 aact:AA00155|3end_hypothetical_protein_111949..112099_POSITIVE 219 110 61 84 31 aact:AA01987|3end_hypothetical_protein_1350620..1350770_POSITIVE 218 99 52 62 9 aact:AA02810|3end_glycine_hydroxymethyltransferase;_serine_hydroxymethyltransferase_1979524..1979674_POSITIVE 218 110 67 145 110 aact:AA00997|3end_response_regulatory_protein_698560..698710_POSITIVE 213 140 91 59 12 aact:AA02329|3end_conserved_hypothetical_protein_1589993..1590143_POSITIVE 213 111 75 53 12 aact:AA00148|3end_ATP-dependent_RNA_helicase_107843..107993_POSITIVE 212 99 52 95 35 aact:AA02130|3end_hypothetical_protein_1454301..1454451_POSITIVE 211 124 84 68 25 aact:AA01816|3end_hypothetical_protein_1224134..1224284_POSITIVE 209 104 64 132 80 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 209 109 68 77 30 aact:AA00252|3end_conserved_hypothetical_protein_177526..177676_POSITIVE 208 104 67 34 3 aact:AA02146|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469477..1469627_POSITIVE