Origin IGS: taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattttaatcctgtaaatagaatcgaagatcaacatattgctcgcaacaaacaatatggcggaaagttagcactttgttaataaaaaacaacacattttttatagaatcgtgaccttgcgcaaaaaaagtgcagtgaaaataccaaaaattttcaccgcacttttcaatggaaagctaaattttatattta Mask Tandem Repeat Region ================================================ taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Find is-nt database================================================ Query_seq: AA00135:AA00136|AA00135:AA00136:S-adenosylmethionine: 2-demethylmenaquinone methyltransferase (menaquinone biosynthesis protein):sodium/sulphate transporter:->->:96941..97131 191 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00135:AA00136|AA00135:AA00136:S-adenosylmethionine: 2-demethylmenaquinone methyltransferase (menaquinone biosynthesis protein):sodium/sulphate transporter:->->:96941..97131 191 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00135:AA00136|AA00135:AA00136:S-adenosylmethionine: 2-demethylmenaquinone methyltransferase (menaquinone biosynthesis protein):sodium/sulphate transporter:->->:96941..97131 191 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 23 End: 184 ...................... M E K C G E N F W Y F H C T F F A Q G H D S I K N V L F F I N K V L T F R H I V C C E Q Y V D L R F Y L Q D ....... Protein_Len: 32 Strand: - Start: 2 End: 97 . F I F N L K G N F L A T F I K P I K V A S K K R L T V I R Y F M .............................................................................................. taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 110 End: 139 .............................................................................................................ATTAACAAAGTGCTAACTTTCCGCCATATT.................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.5 Tail_Score: -5.40072 Start: 31 End: 63 Full_Region: GCTTTCCATTGAAAA GTGCGGTGAAAAT TTTTGGT ATTTTCACTGCAC TTTTTTTGCGCAAGG ..............................GTGCGGTGAAAATTTTTGGTATTTTCACTGCAC................................................................................................................................ TransTerm Strand: - Conf: 88 HP_score: -9.3 Tail_Score: -4.18018 Start: 31 End: 63 Full_Region: ccttgcgcaaaaaaa gtgcagtgaaaat accaaaa attttcaccgcac ttttcaatggaaagc ..............................gtgcagtgaaaataccaaaaattttcaccgcac................................................................................................................................ TransTerm Strand: + Conf: 76 HP_score: -8.7 Tail_Score: -3.39931 Start: 134 End: 158 Full_Region: GTGCTAACTTTCCGC CATATTGTTTG TTG CGAGCAATATG TTGATCTTCGATTCT .....................................................................................................................................CATATTGTTTGTTGCGAGCAATATG................................. Find igs database================================================ Query_seq: AA00135:AA00136|AA00135:AA00136:S-adenosylmethionine: 2-demethylmenaquinone methyltransferase (menaquinone biosynthesis protein):sodium/sulphate transporter:->->:96941..97131 191 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Intra-Species Hit: Count: 1 Min: 1 Max: 191 Len: 191 Subject: aact_AA00135_AA00136|S-adenosylmethionine: 2-demethylmenaquinone methyltransferase (menaquinone biosynthesis protein):sodium/sulphate transporter|POSITIVE:POSITIVE|[96941,97131]|191 HSP 1 e-value: 8.0E-92 bit: 337.0 Len: 191 Query Start:1 Query End:191 Subject Strand: POSITIVE Subject Start: 1 Subject End: 191 taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcacnnnnnnngcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt taaatataaaatttagctttccattgaaaagtgcggtgaaaatttttggtattttcactgcactttttttgcgcaaggtcacgattctataaaaaatgtgttgttttttattaacaaagtgctaactttccgccatattgtttgttgcgagcaatatgttgatcttcgattctatttacaggattaaaatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUAAAAUUUAGCUUUCCAUUGAAAAGUGCGGUGAAAAUUUUUGGUAUUUUCACUGCACUUUUUUUGCGCAAGGUCACGAUUCUAUAAAAAAUGUGUUGUUUUUUAUUAACAAAGUGCUAACUUUCCGCCAUAUUGUUUGUUGCGAGCAAUAUGUUGAUCUUCGAUUCUAUUUACAGGAUUAAAAUU ...................(((...((((((((((((((((((.......))))))))))))))))))...((.(((((((.......(((((((((.....)))))))))....(((((....)))))....(((((((((((...))))))))))).)))))))))............)))........ (-48.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUAAUCCUGUAAAUAGAAUCGAAGAUCAACAUAUUGCUCGCAACAAACAAUAUGGCGGAAAGUUAGCACUUUGUUAAUAAAAAACAACACAUUUUUUAUAGAAUCGUGACCUUGCGCAAAAAAAGUGCAGUGAAAAUACCAAAAAUUUUCACCGCACUUUUCAAUGGAAAGCUAAAUUUUAUAUUUA ........(((......................(((((((...........)))))))(((.((.((((((.....)))))).........((((((((....))))).)))...)).)))...((((((((.((((((((.......)))))))).))))))))....)))................... (-34.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 483 69 27 160 116 aact:AA02244|5end_conserved_hypothetical_protein_(probable_DNA-binding_protein;_death-on-curing_family_protein0_1526402..1526632_POSITIVE 462 69 21 153 105 aact:AA01640|5end_restriction_enzyme_type_I_helicase_subunit_1109230..1109460_POSITIVE 462 69 21 59 11 aact:AA01639|5end_hypothetical_protein_1109136..1109366_POSITIVE 423 67 23 152 106 aact:AA02405|5end_conserved_hypothetical_protein_1652430..1652660_POSITIVE 423 67 23 100 54 aact:AA02404|5end_hypothetical_protein_1652378..1652608_POSITIVE 423 70 21 165 115 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 421 80 31 212 157 aact:AA03000|5end_uracil-DNA_glycosylase_(UDG)_2095076..2095306_POSITIVE 408 67 27 75 32 aact:AA02037|5end_hypothetical_protein_1386832..1387062_POSITIVE 404 79 31 186 140 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 394 68 22 83 34 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 392 80 31 199 142 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 388 67 21 135 100 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 383 78 31 219 170 aact:AA01621|5end_replicative_DNA_helicase_1095647..1095877_POSITIVE 383 78 31 85 36 aact:AA01620|5end_hypothetical_protein_1095513..1095743_POSITIVE 376 68 21 81 31 aact:AA02213|5end_hypothetical_protein_1506996..1507226_POSITIVE 376 80 31 228 175 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 370 68 21 170 126 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 370 68 21 80 36 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 365 69 23 203 151 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 365 67 25 95 51 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 483 69 27 89 45 aact:AA02243|3end_conserved_hypothetical_protein_1526411..1526561_POSITIVE 423 67 23 84 38 aact:AA02402|3end_O-sialoglycoprotein_endopeptidase_1652442..1652592_POSITIVE 423 70 21 85 35 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 419 69 23 89 41 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 408 67 27 68 25 aact:AA02035|3end_translation_initiation_factor_IF-3_1386905..1387055_POSITIVE 392 80 31 68 11 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 388 67 21 82 47 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 383 78 31 70 21 aact:AA01619|3end_phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthase)_1095578..1095728_POSITIVE 376 68 21 83 33 aact:AA02212|3end_ribonucleoside-diphosphate_reductase_beta_chain_1507078..1507228_POSITIVE 365 69 23 102 50 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 365 67 25 75 31 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 359 69 23 84 50 aact:AA01209|3end_conserved_hypothetical_protein_827007..827157_POSITIVE 355 68 26 83 39 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 337 70 28 144 100 aact:AA02862|3end_hypothetical_protein_2008848..2008998_POSITIVE 336 67 21 73 39 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 336 73 37 150 113 aact:AA02648|3end_lytic_transglycosylase_1845637..1845787_POSITIVE 334 79 31 94 45 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 332 77 31 73 29 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 329 67 27 80 38 aact:AA00799|3end_iron(III)_dicitrate_transport_ATP-binding_protein_548502..548652_POSITIVE 329 68 27 84 54 aact:AA00689|3end_GTP-binding_protein_472042..472192_POSITIVE