Origin IGS: aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggtggaaaaagtgcggtcaaaaaacacaatgaattttaaccgcactttgacatattaaacgacgcgccgagtgaacattggcattatgatttattttggtataatattttatacatt Mask Tandem Repeat Region ================================================ aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Find is-nt database================================================ Query_seq: AA00125:AA00126|AA00125:AA00126:conserved hypothetical protein (possible phage-related protein):conserved hypothetical protein:->->:91074..91192 119 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00125:AA00126|AA00125:AA00126:conserved hypothetical protein (possible phage-related protein):conserved hypothetical protein:->->:91074..91192 119 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00125:AA00126|AA00125:AA00126:conserved hypothetical protein (possible phage-related protein):conserved hypothetical protein:->->:91074..91192 119 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 6 End: 98 ..... M K Y Y T K I N H N A N V H S A R R L I C Q S A V K I H C V F ..................... aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00125:AA00126|AA00125:AA00126:conserved hypothetical protein (possible phage-related protein):conserved hypothetical protein:->->:91074..91192 119 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: aact_AA00125_AA00126|conserved hypothetical protein (possible phage-related protein):conserved hypothetical protein|POSITIVE:POSITIVE|[91074,91192]|119 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta aatgtataaaatattataccaaaataaatcataatgccaatgttcactcggcgcgtcgtttaatatgtcaaagtgcggttaaaattcattgtgttttttgaccgcactttttccaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGUAUAAAAUAUUAUACCAAAAUAAAUCAUAAUGCCAAUGUUCACUCGGCGCGUCGUUUAAUAUGUCAAAGUGCGGUUAAAAUUCAUUGUGUUUUUUGACCGCACUUUUUCCACCUA ...((((((....)))))).....(((((.((..((((...........)))).)).))))).......(((((((((((((((..(.....)..)))))))))))))))......... (-26.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUGGAAAAAGUGCGGUCAAAAAACACAAUGAAUUUUAACCGCACUUUGACAUAUUAAACGACGCGCCGAGUGAACAUUGGCAUUAUGAUUUAUUUUGGUAUAAUAUUUUAUACAUU ...(((...((((((((((.((((..(.....)..)))).))))))))))..)))............((((((....).)))))................((((((....))))))... (-29.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 677 110 61 149 100 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 604 110 70 152 112 aact:AA00124|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90270..90500_POSITIVE 604 110 70 103 63 aact:AA00123|5end_hypothetical_protein_90221..90451_POSITIVE 604 110 70 94 54 aact:AA00122|5end_hypothetical_protein_90212..90442_POSITIVE 567 110 70 158 118 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 558 110 71 40 1 aact:AA00758|5end_hypothetical_protein_520485..520715_POSITIVE 539 110 70 156 116 aact:AA00473|5end_tryptophanyl-tRNA_synthetase_328996..329226_POSITIVE 504 110 69 162 121 aact:AA02340|5end_hypothetical_protein_1598887..1599117_POSITIVE 504 110 70 150 110 aact:AA02084|5end_conserved_hypothetical_protein_1417843..1418073_POSITIVE 497 110 70 201 159 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 497 110 70 48 6 aact:AA01829|5end_hypothetical_protein_1231403..1231633_POSITIVE 490 110 70 188 146 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 457 110 70 205 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 456 110 70 224 184 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 456 110 70 94 54 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 448 110 70 154 114 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 447 110 70 156 115 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 442 110 70 142 102 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 437 106 67 158 117 aact:AA00625|5end_hypothetical_protein_430565..430795_POSITIVE 437 106 67 50 9 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 604 110 70 83 43 aact:AA00120|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_90281..90431_POSITIVE 549 110 70 86 46 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 539 110 70 63 23 aact:AA00471|3end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328983..329133_POSITIVE 504 110 69 80 39 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 504 110 70 75 35 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 490 110 70 84 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 461 110 70 78 38 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 457 110 70 44 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 456 110 70 82 42 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 448 110 70 75 35 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 447 110 70 50 9 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 437 106 67 145 104 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 433 110 70 68 28 aact:AA01963|3end_dnaK_suppressor_protein_1329438..1329588_POSITIVE 428 110 66 147 99 aact:AA01068|3end_hypothetical_protein_739850..740000_POSITIVE 419 110 70 86 46 aact:AA02243|3end_conserved_hypothetical_protein_1526411..1526561_POSITIVE 417 110 70 79 39 aact:AA00799|3end_iron(III)_dicitrate_transport_ATP-binding_protein_548502..548652_POSITIVE 404 110 64 76 32 aact:AA01922|3end_translation_initiation_factor_IF2_1297279..1297429_POSITIVE 400 110 64 57 10 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 397 110 70 63 23 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 396 110 70 81 41 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE