Origin IGS: tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaaattgcctttgctgaattgctattccgcgaaaaatcaaaagtgccgatattcgttcggaaaaatgaccgcacttttgagcgcgacaaatgaatcttttctgttgctaaggcta Mask Tandem Repeat Region ================================================ tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Find is-nt database================================================ Query_seq: AA00116:AA00117|AA00116:AA00117:conserved hypothetical protein:hypothetical protein:->->:88268..88385 118 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00116:AA00117|AA00116:AA00117:conserved hypothetical protein:hypothetical protein:->->:88268..88385 118 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00116:AA00117|AA00116:AA00117:conserved hypothetical protein:hypothetical protein:->->:88268..88385 118 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 21 End: 116 .................... M H L S R S K V R S F F R T N I G T F D F S R N S N S A K A I F .. Protein_Len: 31 Strand: + Start: 25 End: 117 ........................ M C R A Q K C G H F S E R I S A L L I F R G I A I Q Q R Q F F . Protein_Len: 37 Strand: - Start: 1 End: 111 L R L L L F S E N T A S L L A T M K G F S Y R C K Q N K A S Y C N L L P M ....... tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00116:AA00117|AA00116:AA00117:conserved hypothetical protein:hypothetical protein:->->:88268..88385 118 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Intra-Species Hit: Count: 1 Min: 1 Max: 118 Len: 118 Subject: aact_AA00116_AA00117|conserved hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[88268,88385]|118 HSP 1 e-value: 5.0E-61 bit: 234.0 Len: 118 Query Start:1 Query End:118 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca tagccttagcaacagaaaagattcatttgtcgcgctcaaaagtgcggtcatttttccgaacgaatatcggcacttttgatttttcgcggaatagcaattcagcaaaggcaatttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCUUAGCAACAGAAAAGAUUCAUUUGUCGCGCUCAAAAGUGCGGUCAUUUUUCCGAACGAAUAUCGGCACUUUUGAUUUUUCGCGGAAUAGCAAUUCAGCAAAGGCAAUUUUUCA ..(((((.((....(((....))).....(((((.((((((((((.(..((((........))))..).)))))))))).....)))))............)).)))))......... (-31.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAAUUGCCUUUGCUGAAUUGCUAUUCCGCGAAAAAUCAAAAGUGCCGAUAUUCGUUCGGAAAAAUGACCGCACUUUUGAGCGCGACAAAUGAAUCUUUUCUGUUGCUAAGGCUA .........((((((((.((((....)))).))))....((((((((((.(....(((((......)))))).))))))))))..((((((...(((....)))))))))..)))).. (-31.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 409 62 31 226 191 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 394 71 31 188 142 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 390 71 31 147 106 aact:AA00148|5end_ATP-dependent_RNA_helicase_105955..106185_POSITIVE 388 50 2 63 29 aact:AA01751|5end_conserved_hypothetical_protein_1184620..1184850_POSITIVE 384 59 24 142 104 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 377 58 13 99 42 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 368 50 2 218 173 aact:AA02426|5end_uridylyltransferase_1671663..1671893_POSITIVE 366 75 33 199 153 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 360 71 32 46 1 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 356 80 39 192 146 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 355 89 41 87 43 aact:AA01853|5end_hypothetical_protein_1246458..1246688_POSITIVE 354 89 41 168 108 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 354 89 41 67 7 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 349 72 32 66 26 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 348 75 40 166 123 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 344 52 18 140 102 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 344 52 18 64 26 aact:AA02835|5end_hypothetical_protein_1991701..1991931_POSITIVE 340 86 41 153 81 aact:AA02335|5end_6-O-methylguanine-DNA_methyltransferase_1596814..1597044_POSITIVE 339 59 13 174 111 aact:AA00311|5end_L-fuculose-phosphate_aldolase_211434..211664_POSITIVE 334 62 34 116 90 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 71 31 94 48 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 390 71 31 56 15 aact:AA00146|3end_UDP-glucose/GDP-mannose_dehydrogenase_105944..106094_POSITIVE 384 59 24 61 23 aact:AA02883|3end_conserved_hypothetical_protein_2022427..2022577_POSITIVE 368 50 2 136 91 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 366 75 33 98 52 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 356 80 39 88 42 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 355 89 41 100 56 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 354 89 41 102 42 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 348 75 40 60 17 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 344 52 18 51 13 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 340 86 41 73 1 aact:AA02334|3end_outer_membrane_protein_P1_1596814..1596964_POSITIVE 330 77 31 82 33 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 325 59 12 109 56 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 320 72 31 62 21 aact:AA02412|3end_cysteine_synthetase_1658552..1658702_POSITIVE 320 58 13 71 15 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 318 90 42 82 37 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 316 49 2 77 32 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 316 62 26 71 34 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 315 109 61 44 3 aact:AA00324|3end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_221321..221471_POSITIVE 313 77 38 82 40 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE