Origin IGS: aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgttattaccgtcatgtgtttttcattaaggcaaaattttagtctctttgttacttaaattgaagtattttcatagcaatacatgaggttatcaccgttccttatgagaattaagattttttatttgcatttagaggcattaattttaataatgtggttgttttttgaaaaatgtattccgactgtagcaataagtaaatttgtttttcaactcggattaaaggaagtttt Mask Tandem Repeat Region ================================================ aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Find is-nt database================================================ Query_seq: AA00115:AA00116|AA00115:AA00116:glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):conserved hypothetical protein:->->:86438..86668 231 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00115:AA00116|AA00115:AA00116:glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):conserved hypothetical protein:->->:86438..86668 231 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00115:AA00116|AA00115:AA00116:glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):conserved hypothetical protein:->->:86438..86668 231 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 14 End: 118 ............. M R V E K Q I Y L L L Q S E Y I F Q K T T T L L K L M P L N A N K K S ................................................................................................................. Protein_Len: 49 Strand: - Start: 3 End: 149 .. V E K L G L Q F V F K S I A V T P I C K E F F L W M I L I L A E L H L Y F I K I R M L F P S L R M .................................................................................. aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 60 End: 89 ...........................................................TTTTTCAAAAAACAACCACATTATTAAAAT.............................................................................................................................................. PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 79 End: 108 ..............................................................................TTTGCATTTAGAGGCATTAATTTTAATAAT........................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00115:AA00116|AA00115:AA00116:glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):conserved hypothetical protein:->->:86438..86668 231 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Intra-Species Hit: Count: 1 Min: 1 Max: 231 Len: 231 Subject: aact_AA00115_AA00116|glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):conserved hypothetical protein|POSITIVE:POSITIVE|[86438,86668]|231 HSP 1 e-value: 1.0E-128 bit: 458.0 Len: 231 Query Start:1 Query End:231 Subject Strand: POSITIVE Subject Start: 1 Subject End: 231 aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca aaaacttcctttaatccgagttgaaaaacaaatttacttattgctacagtcggaatacatttttcaaaaaacaaccacattattaaaattaatgcctctaaatgcaaataaaaaatcttaattctcataaggaacggtgataacctcatgtattgctatgaaaatacttcaatttaagtaacaaagagactaaaattttgccttaatgaaaaacacatgacggtaataaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAACUUCCUUUAAUCCGAGUUGAAAAACAAAUUUACUUAUUGCUACAGUCGGAAUACAUUUUUCAAAAAACAACCACAUUAUUAAAAUUAAUGCCUCUAAAUGCAAAUAAAAAAUCUUAAUUCUCAUAAGGAACGGUGAUAACCUCAUGUAUUGCUAUGAAAAUACUUCAAUUUAAGUAACAAAGAGACUAAAAUUUUGCCUUAAUGAAAAACACAUGACGGUAAUAACA ...................(((....)))........((((((((...(((.(((.......)))............(((((.(((((((...(.((((.((((((.........(((((.......)))))...(((....)))...))))))..........(((((......)))))....)))).)...)))))))...)))))..........))))))))))).. (-28.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUAUUACCGUCAUGUGUUUUUCAUUAAGGCAAAAUUUUAGUCUCUUUGUUACUUAAAUUGAAGUAUUUUCAUAGCAAUACAUGAGGUUAUCACCGUUCCUUAUGAGAAUUAAGAUUUUUUAUUUGCAUUUAGAGGCAUUAAUUUUAAUAAUGUGGUUGUUUUUUGAAAAAUGUAUUCCGACUGUAGCAAUAAGUAAAUUUGUUUUUCAACUCGGAUUAAAGGAAGUUUU ........((...(((..(((((((..(((((((.......((((((.(((.....(((((.....((((((((((....((.(((.....)))..))...))))))))))...))))).......)))...))))))....(((.....)))....))))))).)))))))..)))(((((.((.(((((.........)))))...))..))))).....))....... (-40.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 172 135 160 127 aact:AA01099|5end_dimethyladenosine_transferase_758542..758772_POSITIVE 236 106 65 71 13 aact:AA02305|5end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1574778..1575008_POSITIVE 234 170 133 101 63 aact:AA01140|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_781610..781840_POSITIVE 233 161 124 69 21 aact:AA01510|5end_ABC_transporter,_ATP-binding_protein/permease_1016430..1016660_POSITIVE 220 64 40 61 40 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 219 158 121 228 180 aact:AA01740|5end_conserved_hypothetical_protein_1176558..1176788_POSITIVE 214 162 126 76 35 aact:AA02405|5end_conserved_hypothetical_protein_1652430..1652660_POSITIVE 213 53 12 132 82 aact:AA01739|5end_conserved_hypothetical_protein_1175578..1175808_POSITIVE 212 157 125 198 156 aact:AA02050|5end_1-deoxy-D-xylulose_5-phosphate_synthase_1395625..1395855_POSITIVE 212 157 125 99 57 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 211 159 111 57 7 aact:AA02246|5end_peptidase_E_1527574..1527804_POSITIVE 211 162 125 136 100 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 209 55 11 231 201 aact:AA02824|5end_conserved_hypothetical_protein_1986131..1986361_POSITIVE 208 145 102 229 183 aact:AA01386|5end_30S_ribosomal_protein_S1_935974..936204_POSITIVE 207 156 130 31 1 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 206 160 125 65 16 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 206 53 15 221 188 aact:AA00156|5end_hypothetical_protein_111933..112163_POSITIVE 206 53 15 35 2 aact:AA00155|5end_hypothetical_protein_111747..111977_POSITIVE 204 53 18 174 132 aact:AA00892|5end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_618023..618253_POSITIVE 204 154 113 107 62 aact:AA00646|5end_2-dehydro-3-deoxyphosphooctonate_aldolase_441474..441704_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 172 135 141 108 aact:AA01098|3end_bis(5'-nucleosyl)-tetraphosphatase_758603..758753_POSITIVE 236 106 65 67 9 aact:AA02303|3end_ATP-dependent_DNA_helicase_1574854..1575004_POSITIVE 234 170 133 96 58 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 233 161 124 73 25 aact:AA01509|3end_ABC_system_transport_protein,_ATP_binding/permease_1016514..1016664_POSITIVE 220 64 40 43 22 aact:AA02130|3end_hypothetical_protein_1454301..1454451_POSITIVE 220 175 132 131 95 aact:AA01922|3end_translation_initiation_factor_IF2_1297279..1297429_POSITIVE 219 158 121 102 54 aact:AA01739|3end_conserved_hypothetical_protein_1176512..1176662_POSITIVE 214 156 116 62 15 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 212 157 125 72 30 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 207 44 1 137 96 aact:AA02020|3end_conserved_hypothetical_protein_(possible_tetrapyrrole_methylase_family_protein)_1374742..1374892_POSITIVE 206 53 15 40 7 aact:AA00153|3end_conserved_hypothetical_protein_111832..111982_POSITIVE 204 53 18 125 83 aact:AA00890|3end_conserved_hypothetical_protein_618054..618204_POSITIVE 202 80 41 52 20 aact:AA02869|3end_hypothetical_protein_2013972..2014122_POSITIVE 201 160 128 37 2 aact:AA00961|3end_conserved_hypothetical_protein_671134..671284_POSITIVE 200 150 101 108 54 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 200 75 46 119 82 aact:AA01902|3end_conserved_hypothetical_protein_1283616..1283766_POSITIVE 199 139 91 108 52 aact:AA01901|3end_hypothetical_protein_1282870..1283020_POSITIVE 199 159 123 35 5 aact:AA00694|3end_dCTP_deaminase_(deoxycytidine_triphosphate_deaminas)_475100..475250_POSITIVE 198 65 32 128 90 aact:AA02630|3end_hypothetical_protein_1830474..1830624_POSITIVE 197 140 91 114 63 aact:AA01598|3end_conserved_hypothetical_protein_1079966..1080116_POSITIVE