Origin IGS: tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattttctattaacataaaaataaagagcgaattcagagattcgtctttgattttttattttgctagtgcgcacaaaggcgctttagattataatcgtgcaaatttttgcgtcacttttataggatacatttca Mask Tandem Repeat Region ================================================ tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Find is-nt database================================================ Query_seq: AA00114:AA00115|AA00114:AA00115:peptidyl-prolyl cis-trans isomerase D (PPIase D) (rotamase D):glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):->->:84032..84166 135 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00114:AA00115|AA00114:AA00115:peptidyl-prolyl cis-trans isomerase D (PPIase D) (rotamase D):glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):->->:84032..84166 135 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00114:AA00115|AA00114:AA00115:peptidyl-prolyl cis-trans isomerase D (PPIase D) (rotamase D):glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):->->:84032..84166 135 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: - Start: 1 End: 126 S I Y G I F T V C F N A R N Y D L A G K H A S A F Y F I L S S D R F E S K I K I N M ......... tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 45 HP_score: -3.3 Tail_Score: -3.04078 Start: 91 End: 106 Full_Region: ATAAAAAATCAAAGA CGAAT CTCTGA ATTCG CTCTTTATTTTTATG ..........................................................................................CGAATCTCTGAATTCG............................. Find igs database================================================ Query_seq: AA00114:AA00115|AA00114:AA00115:peptidyl-prolyl cis-trans isomerase D (PPIase D) (rotamase D):glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS):->->:84032..84166 135 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 135 Len: 135 Subject: aact_AA00114_AA00115|peptidyl-prolyl cis-trans isomerase D (PPIase D) (rotamase D):glutamate--cysteine ligase (gamma-glutamylcysteine synthetase) (gamma-ECS)|POSITIVE:POSITIVE|[84032,84166]|135 HSP 1 e-value: 4.0E-71 bit: 268.0 Len: 135 Query Start:1 Query End:135 Subject Strand: POSITIVE Subject Start: 1 Subject End: 135 tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta tgaaatgtatcctataaaagtgacgcaaaaatttgcacgattataatctaaagcgcctttgtgcgcactagcaaaataaaaaatcaaagacgaatctctgaattcgctctttatttttatgttaatagaaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAUGUAUCCUAUAAAAGUGACGCAAAAAUUUGCACGAUUAUAAUCUAAAGCGCCUUUGUGCGCACUAGCAAAAUAAAAAAUCAAAGACGAAUCUCUGAAUUCGCUCUUUAUUUUUAUGUUAAUAGAAAAUUA .....((..((((.....)).))..))....(((((..(((....)))....((((......))))....)))))...((((((.((((((((((......))))).)))))))))))................. (-23.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUCUAUUAACAUAAAAAUAAAGAGCGAAUUCAGAGAUUCGUCUUUGAUUUUUUAUUUUGCUAGUGCGCACAAAGGCGCUUUAGAUUAUAAUCGUGCAAAUUUUUGCGUCACUUUUAUAGGAUACAUUUCA .....((((((.......(((((((((((.....(((((((....))))))))))))))))))...((((((((.(((.((((.(((.....)))..))))...))).)))).))))...))))))......... (-28.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 73 33 208 168 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 274 96 51 59 6 aact:AA01642|5end_possible_iron(III)_ABC_transporter_1110970..1111200_POSITIVE 270 110 64 102 53 aact:AA00570|5end_hypothetical_protein_391613..391843_POSITIVE 269 69 25 202 167 aact:AA02052|5end_probable_exodeoxyribonuclease_VII_small_subunit_1398461..1398691_POSITIVE 265 73 34 66 20 aact:AA00581|5end_thiosulfate_sulfurtransferase_(rhodanese)_397605..397835_POSITIVE 264 76 47 42 18 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 261 77 31 148 99 aact:AA01182|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_809210..809440_POSITIVE 249 68 24 204 162 aact:AA01203|5end_diaminopimelate_epimerase_824140..824370_POSITIVE 247 66 23 117 70 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 247 66 23 48 1 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 246 107 61 165 118 aact:AA02135.1|5end_hypothetical_protein_1458812..1459042_POSITIVE 246 67 21 161 114 aact:AA01152|5end_sec-independent_protein_translocase_protein_789364..789594_POSITIVE 245 69 25 228 186 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 244 70 21 137 84 aact:AA00382|5end_hypothetical_protein_258593..258823_POSITIVE 243 78 37 180 140 aact:AA02383|5end_oxidoreductase_1631255..1631485_POSITIVE 241 72 34 165 116 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 238 66 21 229 186 aact:AA01629|5end_phosphatidylglycerophosphatase_A_1101881..1102111_POSITIVE 238 107 61 83 24 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 237 66 25 47 13 aact:AA00650|5end_15_kDa_peptidoglycan-associated_protein_444498..444728_POSITIVE 237 76 32 75 27 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 73 33 131 91 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 274 68 21 143 92 aact:AA02917|3end_cystathionine_gamma-synthase_2043280..2043430_POSITIVE 257 72 31 144 103 aact:AA01181|3end_conserved_hypothetical_protein_809294..809444_POSITIVE 252 60 18 136 101 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 246 107 61 140 93 aact:AA02134|3end_aminopeptidase_N_1458867..1459017_POSITIVE 246 67 21 139 92 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 241 59 11 137 88 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 238 72 34 149 105 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 238 107 61 76 17 aact:AA00863|3end_conserved_hypothetical_protein_598678..598828_POSITIVE 237 76 32 75 27 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 232 66 24 73 37 aact:AA00124|3end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90670..90820_POSITIVE 229 80 37 136 84 aact:AA01140|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_782526..782676_POSITIVE 229 107 63 120 69 aact:AA00092|3end_serine_transporter_69716..69866_POSITIVE 228 67 24 136 85 aact:AA00087|3end_possible_hydrolase_(alpha/beta_fold_family)_66022..66172_POSITIVE 227 80 38 44 8 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 226 97 53 57 11 aact:AA01789|3end_protease_DegS_precursor;_periplasmic_serine_endoprotease_1207002..1207152_POSITIVE 225 64 37 145 113 aact:AA01695|3end_carbohydrate_transport_protein_1152294..1152444_POSITIVE 224 77 47 25 1 aact:AA01674|3end_hypothetical_protein_1132195..1132345_POSITIVE 220 65 21 73 23 aact:AA01986|3end_pyruvate_dehydrogenase_complex_E2_1350500..1350650_POSITIVE 220 67 21 146 98 aact:AA01673|3end_riboflavin_synthase,_alpha_chain_1132093..1132243_POSITIVE