Origin IGS: aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gggagcctcgttattacacattatggcggaaaccgaaattggcgtccttccttttgaagtgacgacaggataaaaatgaggtgcgattgtactgatttcggttgcattaagccagcgattttacgcgtggaaataaaacgctgtaaaaaatgaccgcacttttttgtgatcaggatcgaagaattggaaaaaatcccgctgaaatatcaggaaatcatactggttggttcttttgtttgagcactttgtgagtaaaatccgggtagcgttaaatgttaataaagaggagtcatt Mask Tandem Repeat Region ================================================ aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Find is-nt database================================================ Query_seq: AA00096:AA00099|AA00096:AA00099:L-asparaginase II precursor:conserved hypothetical protein:->->:72467..72760 294 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00096:AA00099|AA00096:AA00099:L-asparaginase II precursor:conserved hypothetical protein:->->:72467..72760 294 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00096:AA00099|AA00096:AA00099:L-asparaginase II precursor:conserved hypothetical protein:->->:72467..72760 294 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 37 End: 171 .................................... M L L T K C S N K R T N Q Y D F L I F Q R D F F Q F F D P D H K K V R S F F T A F Y F H A ........................................................................................................................... Protein_Len: 57 Strand: + Start: 101 End: 271 .................................................................................................... M F S N S S I L I T K K C G H F L Q R F I S T R K I A G L M Q P K S V Q S H L I F I L S S L Q K E G R Q F R F P P ....................... Protein_Len: 32 Strand: + Start: 199 End: 294 ...................................................................................................................................................................................................... M S T I A P H F Y P V V T S K G R T P I S V S A I M C N N E A P Protein_Len: 33 Strand: - Start: 47 End: 145 .............................................. L T S L C F F W G T H N G S I E A P N K G I R R D Q D C F L A T M ..................................................................................................................................................... aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00096:AA00099|AA00096:AA00099:L-asparaginase II precursor:conserved hypothetical protein:->->:72467..72760 294 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Intra-Species Hit: Count: 1 Min: 1 Max: 294 Len: 294 Subject: aact_AA00096_AA00099|L-asparaginase II precursor:conserved hypothetical protein|POSITIVE:POSITIVE|[72467,72760]|294 HSP 1 e-value: 1.0E-165 bit: 583.0 Len: 294 Query Start:1 Query End:294 Subject Strand: POSITIVE Subject Start: 1 Subject End: 294 aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc aatgactcctctttattaacatttaacgctacccggattttactcacaaagtgctcaaacaaaagaaccaaccagtatgatttcctgatatttcagcgggattttttccaattcttcgatcctgatcacaaaaaagtgcggtcattttttacagcgttttatttccacgcgtaaaatcgctggcttaatgcaaccgaaatcagtacaatcgcacctcatttttatcctgtcgtcacttcaaaaggaaggacgccaatttcggtttccgccataatgtgtaataacgaggctccc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUGACUCCUCUUUAUUAACAUUUAACGCUACCCGGAUUUUACUCACAAAGUGCUCAAACAAAAGAACCAACCAGUAUGAUUUCCUGAUAUUUCAGCGGGAUUUUUUCCAAUUCUUCGAUCCUGAUCACAAAAAAGUGCGGUCAUUUUUUACAGCGUUUUAUUUCCACGCGUAAAAUCGCUGGCUUAAUGCAACCGAAAUCAGUACAAUCGCACCUCAUUUUUAUCCUGUCGUCACUUCAAAAGGAAGGACGCCAAUUUCGGUUUCCGCCAUAAUGUGUAAUAACGAGGCUCCC .......((((.(((((((((((...((..(.(((((.............((((..........(((...........(((..(((((....)))).)..)))...........)))....((((((((......)))((((((((.....(((((((((((........)))))).)))))....))))..))))..))))).......)))).............((.((((.((((.....)))))))).))..))))).)..))....))))).)))))).))))..... (-62.45) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGGAGCCUCGUUAUUACACAUUAUGGCGGAAACCGAAAUUGGCGUCCUUCCUUUUGAAGUGACGACAGGAUAAAAAUGAGGUGCGAUUGUACUGAUUUCGGUUGCAUUAAGCCAGCGAUUUUACGCGUGGAAAUAAAACGCUGUAAAAAAUGACCGCACUUUUUUGUGAUCAGGAUCGAAGAAUUGGAAAAAAUCCCGCUGAAAUAUCAGGAAAUCAUACUGGUUGGUUCUUUUGUUUGAGCACUUUGUGAGUAAAAUCCGGGUAGCGUUAAAUGUUAAUAAAGAGGAGUCAUU .((..((((.((((((.(((((.(((((..((((((((((..((((((((.....)))).))))...............(((((....))))))))))))))).......(((.(.((((((((((((.........)))).........(((.((((......)))).)))....(((((....(((.....))).(((.(((((..((((((((.....))))..))))..))))).))).)))))...))))))))).)))..)))))))))))))))).))))..))... (-77.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 179 133 158 104 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 409 175 134 192 142 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 406 166 121 191 148 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 405 210 162 188 137 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 395 180 132 163 111 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 393 180 131 186 127 aact:AA01559|5end_RecA_regulatory_protein,_RecX_1045970..1046200_POSITIVE 386 180 132 226 181 aact:AA01284|5end_heat_shock_protein_(hsp60)_868574..868804_POSITIVE 386 180 133 151 99 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 383 160 112 171 129 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 371 180 132 172 127 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 367 169 121 178 134 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 365 180 132 201 141 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 361 160 111 153 100 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 354 270 227 139 85 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 353 161 134 64 37 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 350 160 130 130 100 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 342 270 222 217 155 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 342 180 131 197 146 aact:AA01686|5end_hypothetical_protein_1137913..1138143_POSITIVE 340 167 132 163 126 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 340 167 132 87 50 aact:AA02835|5end_hypothetical_protein_1991701..1991931_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 210 162 75 24 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 395 180 132 69 17 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 393 180 131 101 42 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 386 180 132 102 57 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 386 180 133 66 14 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 383 160 112 146 104 aact:AA02507|3end_penicillin-insensitive_murein_endopeptidase_A_1741007..1741157_POSITIVE 367 169 121 138 94 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 365 180 132 120 60 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 354 270 227 149 95 aact:AA02438|3end_membrane_protein;_possible_ABC_transporter_permease_protein_1683992..1684142_POSITIVE 353 161 134 46 19 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 345 179 133 151 109 aact:AA02705|3end_hypothetical_protein_1886928..1887078_POSITIVE 342 270 222 135 73 aact:AA01731|3end_multidrug_resistance_protein_B_1170675..1170825_POSITIVE 342 180 131 120 69 aact:AA01685|3end_molybdenum_ABC_transporter,_ATP-binding_protein_1137916..1138066_POSITIVE 340 167 132 74 37 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 338 160 120 90 50 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 336 160 117 81 29 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 335 170 128 122 83 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 329 180 132 107 57 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 328 172 131 82 45 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 327 167 123 84 36 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE