Origin IGS: taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgacgtcccgtttgaggttttataacctacgtttaaatataaccccatacggtaagtatggggttatatttaaaaacactgtgaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgtttaaaaaatggaattttttgtgaccgcacctttta Mask Tandem Repeat Region ================================================ taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Find is-nt database================================================ Query_seq: AA00093:AA00096|AA00093:AA00096:L-serine dehydratase (L-serine deaminase):L-asparaginase II precursor:->->:71234..71419 186 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00093:AA00096|AA00093:AA00096:L-serine dehydratase (L-serine deaminase):L-asparaginase II precursor:->->:71234..71419 186 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00093:AA00096|AA00093:AA00096:L-serine dehydratase (L-serine deaminase):L-asparaginase II precursor:->->:71234..71419 186 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 77 End: 172 ............................................................................ K L S K H I N K V T N K F I V G Y K G Y P T I N L R L N Y F R M .............. taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 90 HP_score: -12.3 Tail_Score: -3.55563 Start: 37 End: 53 Full_Region: tcaccccttcggggc cgttggc ata gccaacg tttaaaaaatggaat ....................................cgttggcatagccaacg..................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -16.3 Tail_Score: -2.5955 Start: 108 End: 154 Full_Region: ATATTTTTCACAGTG TTTTTAAATATAACCCCATAC TTACC GTATGGGGTTATATTTAAACG TAGGTTATAAAACCT ...........................................................................................................TTTTTAAATATAACCCCATACTTACCGTATGGGGTTATATTTAAACG................................ TransTerm Strand: + Conf: 100 HP_score: -15.7 Tail_Score: -3.58269 Start: 116 End: 146 Full_Region: CACAGTGTTTTTAAA TATAACCCCATAC TTACC GTATGGGGTTATA TTTAAACGTAGGTTA ...................................................................................................................TATAACCCCATACTTACCGTATGGGGTTATA........................................ TransTerm Strand: - Conf: 100 HP_score: -15.7 Tail_Score: -3.54129 Start: 116 End: 146 Full_Region: taacctacgtttaaa tataaccccatac ggtaa gtatggggttata tttaaaaacactgtg ...................................................................................................................tataaccccatacggtaagtatggggttata........................................ TransTerm Strand: + Conf: 88 HP_score: -11.2 Tail_Score: -3.82202 Start: 121 End: 141 Full_Region: TGTTTTTAAATATAA CCCCATAC TTACC GTATGGGG TTATATTTAAACGTA ........................................................................................................................CCCCATACTTACCGTATGGGG............................................. TransTerm Strand: - Conf: 88 HP_score: -11.2 Tail_Score: -3.81488 Start: 121 End: 141 Full_Region: tacgtttaaatataa ccccatac ggtaa gtatgggg ttatatttaaaaaca ........................................................................................................................ccccatacggtaagtatgggg............................................. Find igs database================================================ Query_seq: AA00093:AA00096|AA00093:AA00096:L-serine dehydratase (L-serine deaminase):L-asparaginase II precursor:->->:71234..71419 186 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Intra-Species Hit: Count: 1 Min: 1 Max: 186 Len: 186 Subject: aact_AA00093_AA00096|L-serine dehydratase (L-serine deaminase):L-asparaginase II precursor|POSITIVE:POSITIVE|[71234,71419]|186 HSP 1 e-value: 1.0E-101 bit: 369.0 Len: 186 Query Start:1 Query End:186 Subject Strand: POSITIVE Subject Start: 1 Subject End: 186 taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta taaaaggtgcggtcacaaaaaattccattttttaaacgttggctatgccaacggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttcacagtgtttttaaatataaccccatacttaccgtatggggttatatttaaacgtaggttataaaacctcaaacgggacgtcgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGGUGCGGUCACAAAAAAUUCCAUUUUUUAAACGUUGGCUAUGCCAACGGCCCCGAAGGGGUGAAUAAAUCACUUCAGUGAUUUAUGAAUAUUUUUCACAGUGUUUUUAAAUAUAACCCCAUACUUACCGUAUGGGGUUAUAUUUAAACGUAGGUUAUAAAACCUCAAACGGGACGUCGUUA (((((((((..((........))..)))))))))..(((((((...)))))))..((((..(((((..(((((((((....)))))))))...............((..(((((((((((((((((((.....)))))))))))))))))))..)).........)))))...))))......... (-62.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGACGUCCCGUUUGAGGUUUUAUAACCUACGUUUAAAUAUAACCCCAUACGGUAAGUAUGGGGUUAUAUUUAAAAACACUGUGAAAAAUAUUCAUAAAUCACUGAAGUGAUUUAUUCACCCCUUCGGGGCCGUUGGCAUAGCCAACGUUUAAAAAAUGGAAUUUUUUGUGACCGCACCUUUUA .((((......))))..(((((....)))))...(((((((((((((((((((.....))))))))))))))))))).....((((..........(((((((((....)))))))))(((((((....))).(((((((...)))))))....................)))).))))....... (-60.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 678 70 21 226 177 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 639 72 31 132 90 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 617 68 37 225 194 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 578 67 24 190 143 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 575 72 35 43 5 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 559 95 51 210 166 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 502 70 28 193 151 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 486 70 35 37 2 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 465 80 35 167 124 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 353 67 37 164 120 aact:AA00413|5end_conserved_hypothetical_protein_280142..280372_POSITIVE 348 48 3 181 123 aact:AA00899|5end_possible_quaternary_ammonium_compound_resistance_protein_623214..623444_POSITIVE 345 80 38 208 169 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 345 80 38 67 28 aact:AA00117|5end_hypothetical_protein_88246..88476_POSITIVE 331 26 3 227 204 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 330 84 46 167 129 aact:AA02132|5end_dihydroorotate_dehydrogenase_1455089..1455319_POSITIVE 329 84 50 190 163 aact:AA01354|5end_conserved_hypothetical_protein_916489..916719_POSITIVE 320 67 37 163 120 aact:AA02422|5end_conserved_hypothetical_protein_1668014..1668244_POSITIVE 320 67 37 163 120 aact:AA02298|5end_conserved_hypothetical_protein_1570588..1570818_POSITIVE 320 67 37 163 120 aact:AA00622|5end_conserved_hypothetical_protein_429692..429922_POSITIVE 313 64 36 34 3 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 592 72 36 38 1 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 586 76 31 61 19 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 559 95 51 71 27 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 502 70 28 48 6 aact:AA01088|3end_rare_lipoprotein_B_precursor_753483..753633_POSITIVE 437 69 28 128 87 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 348 48 3 93 35 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 330 84 46 150 112 aact:AA02131|3end_conserved_hypothetical_protein_(possible_TrpH_protein)_1455152..1455302_POSITIVE 318 67 37 131 105 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 305 67 26 132 85 aact:AA02987|3end_histidine_triad_protein_homolog_2089194..2089344_POSITIVE 302 48 2 93 45 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 299 70 38 42 3 aact:AA00789|3end_DNA_topoisomerase_III_542545..542695_POSITIVE 295 59 11 151 102 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 290 59 23 140 97 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 289 67 26 56 11 aact:AA02560|3end_integral_membrane_protein_1783630..1783780_POSITIVE 286 67 36 59 28 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 281 65 25 136 97 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 277 68 37 47 7 aact:AA00937|3end_L-xylulokinase_647525..647675_POSITIVE 276 66 37 62 25 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 276 76 36 91 47 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 276 143 102 70 25 aact:AA01116|3end_hypothetical_protein_767840..767990_POSITIVE