Origin IGS: taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attgtctccttttataagttatatttatttttgcgcagatagtagtactgttacgaagggagtgcaaaaggaatttaaaagatttgtgatcgaagtataacttttagagataattttgatagttttgtaacaagttttaatttaacctgttaatattta Mask Tandem Repeat Region ================================================ taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Find is-nt database================================================ Query_seq: AA00091:AA00092|AA00091:AA00092:short-patch reverse transcription protein:serine transporter:->->:68379..68537 159 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00091:AA00092|AA00091:AA00092:short-patch reverse transcription protein:serine transporter:->->:68379..68537 159 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00091:AA00092|AA00091:AA00092:short-patch reverse transcription protein:serine transporter:->->:68379..68537 159 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 19 End: 132 .................. M K T C Y K T I K I I S K S Y T S I T N L L N S F C T P F V T V L L S A Q K ........................... Protein_Len: 32 Strand: - Start: 63 End: 158 .............................................................. V E I V F R K F E K Q V G K T V T S S D A C F Y I Y S I F P S M . taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00091:AA00092|AA00091:AA00092:short-patch reverse transcription protein:serine transporter:->->:68379..68537 159 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Intra-Species Hit: Count: 1 Min: 1 Max: 159 Len: 159 Subject: aact_AA00091_AA00092|short-patch reverse transcription protein:serine transporter|POSITIVE:POSITIVE|[68379,68537]|159 HSP 1 e-value: 2.0E-85 bit: 315.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 1 Subject End: 159 taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat taaatattaacaggttaaattaaaacttgttacaaaactatcaaaattatctctaaaagttatacttcgatcacaaatcttttaaattccttttgcactcccttcgtaacagtactactatctgcgcaaaaataaatataacttataaaaggagacaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAUUAACAGGUUAAAUUAAAACUUGUUACAAAACUAUCAAAAUUAUCUCUAAAAGUUAUACUUCGAUCACAAAUCUUUUAAAUUCCUUUUGCACUCCCUUCGUAACAGUACUACUAUCUGCGCAAAAAUAAAUAUAACUUAUAAAAGGAGACAAU .......(((((((((.......))))))))).................(((((..((((((((....(((.....)))...........((((((.(.......(((....)))........).)))))).....))))))))......))))).... (-20.26) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGUCUCCUUUUAUAAGUUAUAUUUAUUUUUGCGCAGAUAGUAGUACUGUUACGAAGGGAGUGCAAAAGGAAUUUAAAAGAUUUGUGAUCGAAGUAUAACUUUUAGAGAUAAUUUUGAUAGUUUUGUAACAAGUUUUAAUUUAACCUGUUAAUAUUUA ((((((((....((.(((((((((((.(((((((((.(((((.....))))).(....)..)))))))))..........((((...)))).))))))))))).)))))))))).............(((((.(((.......))).)))))....... (-31.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 127 95 57 24 aact:AA00804|5end_fumarate_reductase_flavoprotein_subunit_A_549770..550000_POSITIVE 229 127 87 126 70 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 229 128 84 170 128 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 227 127 88 149 105 aact:AA02959|5end_pyrroline-5-carboxylate_(P5C)_reductase_2070540..2070770_POSITIVE 227 129 90 187 142 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 224 127 89 192 154 aact:AA02600|5end_conserved_hypothetical_protein_1810672..1810902_POSITIVE 224 127 90 142 101 aact:AA01203|5end_diaminopimelate_epimerase_824140..824370_POSITIVE 221 127 86 139 92 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 221 127 87 163 124 aact:AA00805|5end_fumarate_reductase_iron-sulfur_protein_551583..551813_POSITIVE 220 129 89 208 160 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 220 129 89 174 126 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 217 128 88 134 82 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 215 127 100 129 99 aact:AA01472|5end_hypothetical_protein_988896..989126_POSITIVE 214 127 90 167 128 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 212 127 88 154 118 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 210 113 79 197 159 aact:AA00825|5end_P-protein_(chorismate_mutase/prephenate_dehydratase)_567020..567250_POSITIVE 210 113 79 97 59 aact:AA00824|5end_hypothetical_protein_566920..567150_POSITIVE 209 129 88 189 158 aact:AA02254|5end_2-oxoglutarate/malate_translocator_1534846..1535076_POSITIVE 207 127 87 66 36 aact:AA00684|5end_adenylosuccinate_synthase_467486..467716_POSITIVE 206 125 88 220 183 aact:AA01559|5end_RecA_regulatory_protein,_RecX_1045970..1046200_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 224 128 86 47 2 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 224 127 90 129 88 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 220 129 89 132 84 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 217 128 88 107 55 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 214 127 90 125 86 aact:AA02368|3end_conserved_hypothetical_protein_1624286..1624436_POSITIVE 212 127 88 106 70 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 210 113 79 60 22 aact:AA00823|3end_heat_shock_protein_566963..567113_POSITIVE 209 129 88 49 18 aact:AA02253|3end_hypothetical_protein_1534786..1534936_POSITIVE 208 43 7 93 48 aact:AA00634|3end_DNA_polymerase_IV_(Pol_IV)_435831..435981_POSITIVE 206 125 88 135 98 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 204 125 88 120 75 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 200 135 102 134 98 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 199 126 86 102 50 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE 196 127 99 84 56 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 193 126 92 113 75 aact:AA01810|3end_electron_transport_complex_protein_1220406..1220556_POSITIVE 192 117 74 67 27 aact:AA01555|3end_ABC_transporter,_ATP-binding_protein_1044323..1044473_POSITIVE 192 126 95 141 113 aact:AA00619|3end_hypothetical_protein_424324..424474_POSITIVE 190 106 68 36 3 aact:AA00729|3end_possible_hemolysin_(TlyC-like)_501177..501327_POSITIVE 188 132 93 110 72 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 188 126 86 130 90 aact:AA00347|3end_50S_ribosomal_protein_L11_232642..232792_POSITIVE