Origin IGS: taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtgttctccggaatgatgttaattcaatgtaaatgttagcgtttttggaagtatttccatctagacggctataatacgcgtttagctcaaaaatgcaacttggaaaggggagagttttgtcagtttgatgtaaataatgaggtgtataaaaaagaaaacccccaacaaacgttgagggtttctactaaaataacaacctaaaaagatta Mask Tandem Repeat Region ================================================ taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Find is-nt database================================================ Query_seq: AA00088:AA00089|AA00088:AA00089:30S ribosomal protein S15:S-adenosylmethionine synthase; methionine adenosyltransferase:->->:66448..66657 210 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00088:AA00089|AA00088:AA00089:30S ribosomal protein S15:S-adenosylmethionine synthase; methionine adenosyltransferase:->->:66448..66657 210 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00088:AA00089|AA00088:AA00089:30S ribosomal protein S15:S-adenosylmethionine synthase; methionine adenosyltransferase:->->:66448..66657 210 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 3 End: 125 .. M F L G C Y F S R N P Q R L L G V F F F I H L I I Y I K L T K L S P F Q V A F L S ..................................................................................... Protein_Len: 31 Strand: + Start: 116 End: 208 ................................................................................................................... M F E L N A Y Y S R L D G N T S K N A N I Y I E L T S F R R T .. Protein_Len: 33 Strand: - Start: 12 End: 110 ........... T T I K T S V R L T Q Q P N E K K Y V E N N V D F Q C F E G R E M .................................................................................................... taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 76 HP_score: -7.8 Tail_Score: -3.9712 Start: 32 End: 51 Full_Region: gtataaaaaagaaaa cccccaac aaac gttgaggg tttctactaaaataa ...............................cccccaacaaacgttgaggg............................................................................................................................................................... Find igs database================================================ Query_seq: AA00088:AA00089|AA00088:AA00089:30S ribosomal protein S15:S-adenosylmethionine synthase; methionine adenosyltransferase:->->:66448..66657 210 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Intra-Species Hit: Count: 1 Min: 1 Max: 210 Len: 210 Subject: aact_AA00088_AA00089|30S ribosomal protein S15:S-adenosylmethionine synthase; methionine adenosyltransferase|POSITIVE:POSITIVE|[66448,66657]|210 HSP 1 e-value: 1.0E-115 bit: 416.0 Len: 210 Query Start:1 Query End:210 Subject Strand: POSITIVE Subject Start: 1 Subject End: 210 taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca taatctttttaggttgttattttagtagaaaccctcaacgtttgttgggggttttcttttttatacacctcattatttacatcaaactgacaaaactctcccctttccaagttgcatttttgagctaaacgcgtattatagccgtctagatggaaatacttccaaaaacgctaacatttacattgaattaacatcattccggagaacaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCUUUUUAGGUUGUUAUUUUAGUAGAAACCCUCAACGUUUGUUGGGGGUUUUCUUUUUUAUACACCUCAUUAUUUACAUCAAACUGACAAAACUCUCCCCUUUCCAAGUUGCAUUUUUGAGCUAAACGCGUAUUAUAGCCGUCUAGAUGGAAAUACUUCCAAAAACGCUAACAUUUACAUUGAAUUAACAUCAUUCCGGAGAACACA ..........((((.((......((..((((((((((((....))))))))))))..)).....))))))..........................(((((.........(((((.((((((.((.....))(((((....((((....)))).)))))...)))))).)).)))........(((.......)))....)))))..... (-39.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGUUCUCCGGAAUGAUGUUAAUUCAAUGUAAAUGUUAGCGUUUUUGGAAGUAUUUCCAUCUAGACGGCUAUAAUACGCGUUUAGCUCAAAAAUGCAACUUGGAAAGGGGAGAGUUUUGUCAGUUUGAUGUAAAUAAUGAGGUGUAUAAAAAAGAAAACCCCCAACAAACGUUGAGGGUUUCUACUAAAAUAACAACCUAAAAAGAUUA .......((((((..(((((((((...........)))))))))))))))..(((((.((((.(((((((...(((..((((((.......))))))..(((....))).....)))..))).)))))))).)))))...((((.(((.....(((((.(((.((((....)))).))))))))......)))...)))).......... (-44.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 142 101 135 98 aact:AA01710|5end_ribosomal_protein_S6_modification_protein_1159348..1159578_POSITIVE 275 143 101 181 135 aact:AA00938|5end_L-xylulokinase_647556..647786_POSITIVE 272 133 91 219 169 aact:AA00976|5end_conserved_hypothetical_protein_681206..681436_POSITIVE 268 59 27 197 166 aact:AA01801|5end_malate_dehydrogenase_1213358..1213588_POSITIVE 268 59 27 50 19 aact:AA01800|5end_hypothetical_protein_1213211..1213441_POSITIVE 268 159 112 184 144 aact:AA01775|5end_hypothetical_protein_1196001..1196231_POSITIVE 268 69 28 44 2 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 264 148 101 194 144 aact:AA01857|5end_glutamate-ammonia-ligase_adenylyltransferase_1247347..1247577_POSITIVE 263 176 132 79 34 aact:AA00884|5end_sodium-dependent_transporter_614392..614622_POSITIVE 257 146 111 53 9 aact:AA00549|5end_ferredoxin-type_protein_375563..375793_POSITIVE 248 199 152 50 10 aact:AA02858|5end_conserved_hypothetical_protein_2005266..2005496_POSITIVE 248 51 13 110 71 aact:AA00581|5end_thiosulfate_sulfurtransferase_(rhodanese)_397605..397835_POSITIVE 245 49 12 75 35 aact:AA02631|5end_undecaprenyl-phosphate_galactosephosphotransferase_homolog_1830469..1830699_POSITIVE 245 159 113 216 158 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 244 56 31 45 24 aact:AA02599|5end_outer_membrane_protein,_Slp_family_1810083..1810313_POSITIVE 244 154 124 45 17 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 243 57 14 86 47 aact:AA01880|5end_N-acetylglucosamine_repressor_1267083..1267313_POSITIVE 242 134 98 228 191 aact:AA01530|5end_conserved_hypothetical_protein_1027308..1027538_POSITIVE 242 154 112 164 119 aact:AA00761|5end_conserved_hypothetical_protein_(possible_peptidase)_523554..523784_POSITIVE 239 60 11 179 127 aact:AA01905|5end_hypothetical_protein_1285306..1285536_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 142 101 41 4 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 284 163 121 135 92 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 284 57 11 64 21 aact:AA00088|3end_30S_ribosomal_protein_S15_66387..66537_POSITIVE 275 143 101 70 24 aact:AA00937|3end_L-xylulokinase_647525..647675_POSITIVE 272 133 91 60 10 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 268 59 27 35 4 aact:AA01799|3end_polysacharide_biosynthesis_protein_(Orf14_of_cluster)_1213276..1213426_POSITIVE 268 159 112 79 39 aact:AA01774|3end_5,10-methylenetetrahydrofolate_reductase_1195976..1196126_POSITIVE 263 176 132 74 29 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 253 144 119 151 120 aact:AA01437|3end_phosphoglycerate_kinase_965365..965515_POSITIVE 248 51 13 53 14 aact:AA00580|3end_conserved_hypothetical_protein_(possible_membrane_protein)_397628..397778_POSITIVE 247 140 98 107 63 aact:AA01528|3end_probable_oxidoreductase_1027260..1027410_POSITIVE 244 154 124 72 44 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 240 146 101 150 102 aact:AA01855|3end_transcriptional_regulator,_Cro/CI_family_1247385..1247535_POSITIVE 239 148 101 104 43 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 238 124 92 33 3 aact:AA01109|3end_oxidoreductase_(possible_short-chain_alcohol_dehydrogenase)_764725..764875_POSITIVE 237 146 101 61 7 aact:AA01846|3end_conserved_hypothetical_protein_1242165..1242315_POSITIVE 235 170 124 61 18 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 234 159 111 55 4 aact:AA01233|3end_50S_ribosomal_protein_L6_841463..841613_POSITIVE 234 55 12 86 37 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 233 90 43 106 55 aact:AA02420|3end_ISRSO11_related_transposase_1662668..1662818_POSITIVE