Origin IGS: agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgaaaaagcattgtctttactaggaaaataagttaaaaataaaccgtaattctaccgcactttgcgggaatttttcacaaaatatttcatttcttaatcactaaggtcgaaact Mask Tandem Repeat Region ================================================ agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Find is-nt database================================================ Query_seq: AA00084:AA00085|AA00084:AA00085:aminotransferase:menaquinone-specific isochorismate; isochorismate mutase:->->:62166..62281 116 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00084:AA00085|AA00084:AA00085:aminotransferase:menaquinone-specific isochorismate; isochorismate mutase:->->:62166..62281 116 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00084:AA00085|AA00084:AA00085:aminotransferase:menaquinone-specific isochorismate; isochorismate mutase:->->:62166..62281 116 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Predict ORF larger than 30AA ================================================ agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00084:AA00085|AA00084:AA00085:aminotransferase:menaquinone-specific isochorismate; isochorismate mutase:->->:62166..62281 116 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: aact_AA00084_AA00085|aminotransferase:menaquinone-specific isochorismate; isochorismate mutase|POSITIVE:POSITIVE|[62166,62281]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa agtttcgaccttagtgattaagaaatgaaatattttgtgaaaaattcccgcaaagtgcggtagaattacggtttatttttaacttattttcctagtaaagacaatgctttttcaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUUCGACCUUAGUGAUUAAGAAAUGAAAUAUUUUGUGAAAAAUUCCCGCAAAGUGCGGUAGAAUUACGGUUUAUUUUUAACUUAUUUUCCUAGUAAAGACAAUGCUUUUUCAAA .((((..((..(((.((.((((....((((((..((((((....(((((((.....))))..)))))))))..))))))...))))...)))))))..)))).............. (-20.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGAAAAAGCAUUGUCUUUACUAGGAAAAUAAGUUAAAAAUAAACCGUAAUUCUACCGCACUUUGCGGGAAUUUUUCACAAAAUAUUUCAUUUCUUAAUCACUAAGGUCGAAACU ..(((((((..(((.((((....)))).))).........................((((.....))))...))))))).......((((...(((((.....)))))..)))).. (-15.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 79 37 82 42 aact:AA02842|5end_conserved_hypothetical_protein_1996574..1996804_POSITIVE 304 85 46 167 119 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 304 85 46 77 29 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 292 84 46 55 14 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 291 93 52 105 61 aact:AA02436|5end_periplasmic_protein_1680497..1680727_POSITIVE 287 93 52 102 58 aact:AA01209|5end_conserved_hypothetical_protein_826430..826660_POSITIVE 287 74 47 222 185 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 284 72 36 92 61 aact:AA01743|5end_lysyl-tRNA_synthetase_1177808..1178038_POSITIVE 281 85 46 231 198 aact:AA01592|5end_hypothetical_protein_1070950..1071180_POSITIVE 280 80 48 81 46 aact:AA01751|5end_conserved_hypothetical_protein_1184620..1184850_POSITIVE 274 97 52 107 54 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 273 76 37 194 159 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 269 70 31 184 146 aact:AA00039|5end_cytochrome_c-type_biogenesis_protein_30312..30542_POSITIVE 268 77 35 158 108 aact:AA01161|5end_araC-like_transcriptional_regulator_794121..794351_POSITIVE 267 60 13 129 81 aact:AA02128|5end_DNA_topoisomerase_I_1451395..1451625_POSITIVE 260 72 42 58 18 aact:AA02239|5end_molybdopterin_biosynthesis_protein_D_1523216..1523446_POSITIVE 259 86 47 98 55 aact:AA00890|5end_conserved_hypothetical_protein_617075..617305_POSITIVE 257 93 52 231 184 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 255 85 49 192 155 aact:AA01083|5end_preprotein_translocase_SecA_subunit_748131..748361_POSITIVE 253 88 49 83 40 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 84 46 80 39 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 291 93 52 59 15 aact:AA02434|3end_membrane_protein_1680531..1680681_POSITIVE 287 93 52 55 11 aact:AA01207|3end_hypothetical_protein_826463..826613_POSITIVE 274 97 52 95 42 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 270 70 46 151 124 aact:AA01543|3end_hypothetical_protein_1034350..1034500_POSITIVE 268 77 35 147 97 aact:AA01160|3end_ADP_compounds_hydrolase_794190..794340_POSITIVE 261 96 52 92 41 aact:AA02748|3end_conserved_hypothetical_protein_1925582..1925732_POSITIVE 260 72 42 54 14 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 259 86 47 53 10 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 253 88 49 89 46 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 251 75 32 124 79 aact:AA02307|3end_5'-guanylate_kinase_1578058..1578208_POSITIVE 250 96 52 88 46 aact:AA01479|3end_spermidine/putrescine-binding_periplasmic_protein_995828..995978_POSITIVE 248 88 48 96 50 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 247 50 1 51 3 aact:AA01628|3end_thiamin-monophosphate_kinase_1101948..1102098_POSITIVE 245 65 45 71 52 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 243 80 31 95 43 aact:AA00933|3end_ribose_ABC_transporter,_ATP-binding_protein_645197..645347_POSITIVE 243 76 50 39 6 aact:AA00926|3end_CyaY_protein_635891..636041_POSITIVE 242 74 45 43 10 aact:AA02050|3end_1-deoxy-D-xylulose_5-phosphate_synthase_1397552..1397702_POSITIVE 242 74 37 83 38 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 241 82 50 68 37 aact:AA00506|3end_ATP-dependent_RNA_helicase_protein_353089..353239_POSITIVE