Origin IGS: aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtaattcggtattatcagtagttatcaaataaaaaacggctttattatcgttgaaacgactatacattacaattagataattattttttaaattcgttgacatcattttttatctttgctacagtttagcgctgaactaccttgaacggtattaggttaatatattgttaattaagaaattaaggtcaacttt Mask Tandem Repeat Region ================================================ aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Find is-nt database================================================ Query_seq: AA00080:AA00081|AA00080:AA00081:Na+/H+ antiporter protein:fatty acid metabolism regulator protein:->->:59905..60098 194 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00080:AA00081|AA00080:AA00081:Na+/H+ antiporter protein:fatty acid metabolism regulator protein:->->:59905..60098 194 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00080:AA00081|AA00080:AA00081:Na+/H+ antiporter protein:fatty acid metabolism regulator protein:->->:59905..60098 194 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 88 End: 192 ....................................................................................... M M S T N L K N N Y L I V M Y S R F N D N K A V F Y L I T T D N T E L .. aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 87 Start: 69 End: 98 ....................................................................GTTGACATCATTTTTTATCTTTGCTACAGT................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 121 End: 150 ........................................................................................................................ATTGTAATGTATAGTCGTTTCAACGATAAT............................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00080:AA00081|AA00080:AA00081:Na+/H+ antiporter protein:fatty acid metabolism regulator protein:->->:59905..60098 194 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Intra-Species Hit: Count: 1 Min: 1 Max: 194 Len: 194 Subject: aact_AA00080_AA00081|Na+/H+ antiporter protein:fatty acid metabolism regulator protein|POSITIVE:POSITIVE|[59905,60098]|194 HSP 1 e-value: 1.0E-106 bit: 385.0 Len: 194 Query Start:1 Query End:194 Subject Strand: POSITIVE Subject Start: 1 Subject End: 194 aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact aaagttgaccttaatttcttaattaacaatatattaacctaataccgttcaaggtagttcagcgctaaactgtagcaaagataaaaaatgatgtcaacgaatttaaaaaataattatctaattgtaatgtatagtcgtttcaacgataataaagccgttttttatttgataactactgataataccgaattact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGUUGACCUUAAUUUCUUAAUUAACAAUAUAUUAACCUAAUACCGUUCAAGGUAGUUCAGCGCUAAACUGUAGCAAAGAUAAAAAAUGAUGUCAACGAAUUUAAAAAAUAAUUAUCUAAUUGUAAUGUAUAGUCGUUUCAACGAUAAUAAAGCCGUUUUUUAUUUGAUAACUACUGAUAAUACCGAAUUACU ...((((...((((((....))))))))))......................((((..((((.((((.....))))..((((((((((((...................((((((....))))))....(((.(((((....))))).)))....))))))))))))........))))...))))........ (-28.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAAUUCGGUAUUAUCAGUAGUUAUCAAAUAAAAAACGGCUUUAUUAUCGUUGAAACGACUAUACAUUACAAUUAGAUAAUUAUUUUUUAAAUUCGUUGACAUCAUUUUUUAUCUUUGCUACAGUUUAGCGCUGAACUACCUUGAACGGUAUUAGGUUAAUAUAUUGUUAAUUAAGAAAUUAAGGUCAACUUU .................((((((((((.((((((.......)))))).((((....))))................))))))))))..........((((((...((((((((.....((......(((((.((((..((((......)))))))))))))......))....))))))))....))))))... (-31.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 290 100 53 83 31 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 276 100 52 212 167 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 270 78 33 168 121 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 267 82 45 88 58 aact:AA02067|5end_conserved_hypothetical_protein;_possible_membrane_protein_1405233..1405463_POSITIVE 257 72 31 184 148 aact:AA01863|5end_hypothetical_protein_1252011..1252241_POSITIVE 255 71 35 150 119 aact:AA02388|5end_alpha-glucosidase_1636138..1636368_POSITIVE 250 77 39 68 34 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 250 90 45 111 67 aact:AA00732|5end_citrate_lyase_ligase_501343..501573_POSITIVE 248 188 154 123 86 aact:AA00306|5end_nitrogen_regulatory_protein_p-II_207923..208153_POSITIVE 243 77 36 59 19 aact:AA01236|5end_50S_ribosomal_protein_L15_842459..842689_POSITIVE 242 76 46 72 46 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 240 65 27 190 150 aact:AA02850|5end_dATP_pyrophosphohydrolase_2000472..2000702_POSITIVE 236 82 45 72 36 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 235 66 43 90 65 aact:AA02574|5end_dGTP_triphosphohydrolase_1792395..1792625_POSITIVE 233 81 42 143 107 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 227 168 124 231 175 aact:AA00962|5end_exopolyphosphatase_(metaphosphatase)_671060..671290_POSITIVE 227 80 45 219 173 aact:AA00770|5end_outer_membrane_protein_precursor_530788..531018_POSITIVE 224 82 42 215 172 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 223 83 45 230 185 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 221 156 132 223 198 aact:AA00724|5end_hypothetical_protein_497807..498037_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 290 100 53 109 57 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 276 100 52 77 32 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 270 78 33 134 87 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE 267 82 45 89 59 aact:AA02066|3end_hypothetical_protein_1405314..1405464_POSITIVE 255 71 35 123 92 aact:AA02387|3end_integral_membrane_transport_protein_1636191..1636341_POSITIVE 250 90 45 70 26 aact:AA00730|3end_hypothetical_protein_501382..501532_POSITIVE 243 77 36 55 15 aact:AA01235.1|3end_50S_ribosomal_protein_L30_842535..842685_POSITIVE 235 66 43 44 19 aact:AA02573|3end_ABC_transporter,_ATP-binding_protein_1792429..1792579_POSITIVE 233 81 42 143 107 aact:AA01459|3end_Na(+)/H(+)_antiporter_981492..981642_POSITIVE 226 80 33 66 10 aact:AA03009|3end_hypothetical_protein_2101236..2101386_POSITIVE 224 82 42 144 101 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 215 161 122 150 106 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 213 179 132 113 73 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 211 76 38 144 110 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 210 77 32 71 27 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 208 99 53 103 55 aact:AA02350|3end_possible_lipoprotein_1609179..1609329_POSITIVE 208 75 41 136 103 aact:AA01936|3end_tyrosyl-tRNA_synthetase_1308307..1308457_POSITIVE 208 72 43 32 1 aact:AA00140|3end_hypothetical_protein_100872..101022_POSITIVE 205 189 144 48 3 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 199 77 51 29 4 aact:AA01903|3end_hypothetical_protein_1284215..1284365_POSITIVE