Origin IGS: aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttctctccttattgatgaatgaacggaagtcacaacataaccaattcaatcatataagtctagaaataaaatagtaggcgggaaaagggttatggattagaatgacgaaaatttcacttaatcaataggaagggtt Mask Tandem Repeat Region ================================================ aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Find is-nt database================================================ Query_seq: AA00071:AA00072|AA00071:AA00072:conserved hypothetical protein (possible zinc protease):conserved hypothetical protein:->->:54428..54566 139 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00071:AA00072|AA00071:AA00072:conserved hypothetical protein (possible zinc protease):conserved hypothetical protein:->->:54428..54566 139 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00071:AA00072|AA00071:AA00072:conserved hypothetical protein (possible zinc protease):conserved hypothetical protein:->->:54428..54566 139 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Predict ORF larger than 30AA ================================================ aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00071:AA00072|AA00071:AA00072:conserved hypothetical protein (possible zinc protease):conserved hypothetical protein:->->:54428..54566 139 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: aact_AA00071_AA00072|conserved hypothetical protein (possible zinc protease):conserved hypothetical protein|POSITIVE:POSITIVE|[54428,54566]|139 HSP 1 e-value: 2.0E-73 bit: 276.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa aacccttcctattgattaagtgaaattttcgtcattctaatccataacccttttcccgcctactattttatttctagacttatatgattgaattggttatgttgtgacttccgttcattcatcaataaggagagaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCCUUCCUAUUGAUUAAGUGAAAUUUUCGUCAUUCUAAUCCAUAACCCUUUUCCCGCCUACUAUUUUAUUUCUAGACUUAUAUGAUUGAAUUGGUUAUGUUGUGACUUCCGUUCAUUCAUCAAUAAGGAGAGAAAAA ....((((.(((((((..((((((......(((((.......(((((((...(((...........((((....))))...........)))..)))))))..))))).....)))))).))))))).))))....... (-23.75) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUCUCUCCUUAUUGAUGAAUGAACGGAAGUCACAACAUAACCAAUUCAAUCAUAUAAGUCUAGAAAUAAAAUAGUAGGCGGGAAAAGGGUUAUGGAUUAGAAUGACGAAAAUUUCACUUAAUCAAUAGGAAGGGUU .....((((.(((((((((...((((.....((((...(((((((..(((..........(((((............)))))..)))...)))))))........))))......))))....))))))))).)))).. (-28.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 273 59 18 41 4 aact:AA01879|5end_glutamyl-tRNA_reductase_1265717..1265947_POSITIVE 259 128 82 140 89 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 243 59 22 117 85 aact:AA00976|5end_conserved_hypothetical_protein_681206..681436_POSITIVE 240 90 49 202 160 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 240 90 49 168 126 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 239 123 94 172 140 aact:AA02156|5end_conserved_hypothetical_protein_1474581..1474811_POSITIVE 233 50 1 134 75 aact:AA01911|5end_hypothetical_protein_1287791..1288021_POSITIVE 227 60 12 161 113 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 224 60 29 217 181 aact:AA00516|5end_autoinducer-2_production_protein_355931..356161_POSITIVE 222 130 90 130 81 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 221 77 47 174 142 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 220 62 39 214 179 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 220 60 13 198 140 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 219 59 11 105 47 aact:AA02955|5end_trigger_factor_2067022..2067252_POSITIVE 217 60 28 221 181 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 215 62 30 217 186 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 215 130 84 72 26 aact:AA00689|5end_GTP-binding_protein_470433..470663_POSITIVE 215 73 31 44 3 aact:AA00614|5end_ribonuclease_HII_(RNase_HII)_419890..420120_POSITIVE 214 96 51 189 131 aact:AA00718|5end_ABC-related_ATP-binding_protein;_MRP_protein_493534..493764_POSITIVE 214 59 26 69 34 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 128 82 140 89 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 240 90 49 126 84 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 239 123 94 132 100 aact:AA02154|3end_possible_ATP-binding_protein_of_ABC_transporter_1474621..1474771_POSITIVE 227 60 12 66 18 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 226 75 42 136 96 aact:AA00475|3end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_332282..332432_POSITIVE 224 60 29 44 8 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 220 119 72 108 66 aact:AA02869|3end_hypothetical_protein_2013972..2014122_POSITIVE 220 64 43 100 75 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 215 62 30 122 91 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 215 73 31 48 7 aact:AA00613|3end_lipid-A-disaccharide_synthase_419974..420124_POSITIVE 212 117 89 146 117 aact:AA02850|3end_dATP_pyrophosphohydrolase_2000995..2001145_POSITIVE 211 59 19 128 90 aact:AA01603|3end_oligopeptidase_A_1085014..1085164_POSITIVE 210 115 75 103 48 aact:AA00804|3end_fumarate_reductase_flavoprotein_subunit_A_551655..551805_POSITIVE 206 125 87 144 104 aact:AA02171|3end_polysialic_acid_capsule_expression_protein_1486096..1486246_POSITIVE 205 106 65 116 72 aact:AA01031|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_C_721077..721227_POSITIVE 204 115 79 48 19 aact:AA02698|3end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1881425..1881575_POSITIVE 203 97 55 124 78 aact:AA02420|3end_ISRSO11_related_transposase_1662668..1662818_POSITIVE 203 88 50 51 18 aact:AA00061|3end_cytochrome_c-type_biogenesis_ATP-binding_protein_46794..46944_POSITIVE 202 61 36 116 94 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 202 90 41 82 33 aact:AA02150|3end_conserved_hypothetical_protein_1470985..1471135_POSITIVE