Origin IGS: taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaactatgtgataaaaaagtgcggttgtttttaccatcatttcctaaacctgcaataggtaaaccaccgtaagtcaggtaataattctaacttgcggcgtgtagcgtggatttttctacgaaccgattaatggatagaaaaggtggtagaaacccgtgttgaaagcttcagaccacctttacgccgatcaagaggaaaaccgagttta Mask Tandem Repeat Region ================================================ taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Find is-nt database================================================ Query_seq: AA00069:AA00071|AA00069:AA00071:conserved hypothetical protein:conserved hypothetical protein (possible zinc protease):->->:53394..53602 209 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00069:AA00071|AA00069:AA00071:conserved hypothetical protein:conserved hypothetical protein (possible zinc protease):->->:53394..53602 209 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00069:AA00071|AA00069:AA00071:conserved hypothetical protein:conserved hypothetical protein (possible zinc protease):->->:53394..53602 209 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Predict ORF larger than 30AA ================================================ taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 46 HP_score: -4.3 Tail_Score: -2.85503 Start: 110 End: 144 Full_Region: AAAATCCACGCTACA CGCCGCAAGTTAG AATTATTAC CTGACTTACGGTG GTTTACCTATTGCAG .............................................................................................................CGCCGCAAGTTAGAATTATTACCTGACTTACGGTG................................................................. TransTerm Strand: + Conf: 44 HP_score: -3.3 Tail_Score: -2.974 Start: 112 End: 142 Full_Region: AATCCACGCTACACG CCGCAAGTTAG AATTATTAC CTGACTTACGG TGGTTTACCTATTGC ...............................................................................................................CCGCAAGTTAGAATTATTACCTGACTTACGG................................................................... Find igs database================================================ Query_seq: AA00069:AA00071|AA00069:AA00071:conserved hypothetical protein:conserved hypothetical protein (possible zinc protease):->->:53394..53602 209 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Intra-Species Hit: Count: 1 Min: 1 Max: 209 Len: 209 Subject: aact_AA00069_AA00071|conserved hypothetical protein:conserved hypothetical protein (possible zinc protease)|POSITIVE:POSITIVE|[53394,53602]|209 HSP 1 e-value: 1.0E-115 bit: 414.0 Len: 209 Query Start:1 Query End:209 Subject Strand: POSITIVE Subject Start: 1 Subject End: 209 taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta taaactcggttttcctcttgatcggcgtaaaggtggtctgaagctttcaacacgggtttctaccaccttttctatccattaatcggttcgtagaaaaatccacgctacacgccgcaagttagaattattacctgacttacggtggtttacctattgcaggtttaggaaatgatggtaaaaacaaccgcacttttttatcacatagttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACUCGGUUUUCCUCUUGAUCGGCGUAAAGGUGGUCUGAAGCUUUCAACACGGGUUUCUACCACCUUUUCUAUCCAUUAAUCGGUUCGUAGAAAAAUCCACGCUACACGCCGCAAGUUAGAAUUAUUACCUGACUUACGGUGGUUUACCUAUUGCAGGUUUAGGAAAUGAUGGUAAAAACAACCGCACUUUUUUAUCACAUAGUUUA ((((((....((((((((((...(((((..(((((((..(((((((.......))))))).)))))))(((((((((.......))...))))))).....)))))...(((((.(((((((.........))))))).)))))............))))...))))))...(((((((((..........)))))))))...)))))) (-55.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACUAUGUGAUAAAAAAGUGCGGUUGUUUUUACCAUCAUUUCCUAAACCUGCAAUAGGUAAACCACCGUAAGUCAGGUAAUAAUUCUAACUUGCGGCGUGUAGCGUGGAUUUUUCUACGAACCGAUUAAUGGAUAGAAAAGGUGGUAGAAACCCGUGUUGAAAGCUUCAGACCACCUUUACGCCGAUCAAGAGGAAAACCGAGUUUA ((((((...(((......((..(((..((((((((((((.((((....(((((...)))))...(((((((((((.(((.......))).))))))))((.((..((((((....)))))).)))).....)))...)))).)))))))))))))))..)).......))).....(((((..........))))).......)))))) (-57.12) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 378 171 132 131 78 aact:AA02747|5end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1924324..1924554_POSITIVE 348 65 21 163 109 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 341 60 17 168 122 aact:AA00761|5end_conserved_hypothetical_protein_(possible_peptidase)_523554..523784_POSITIVE 339 200 155 170 125 aact:AA00194|5end_oxygen-independent_coproporphyrinogen_III_oxidase_135545..135775_POSITIVE 336 97 53 225 173 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 330 49 4 136 86 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 319 160 112 133 73 aact:AA02658|5end_conserved_hypothetical_protein_1852260..1852490_POSITIVE 311 145 104 66 18 aact:AA00804|5end_fumarate_reductase_flavoprotein_subunit_A_549770..550000_POSITIVE 309 195 155 143 105 aact:AA00266|5end_conserved_hypothetical_protein_(probable_metabolite_transport_protein)_186634..186864_POSITIVE 308 116 77 178 130 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 308 63 22 64 24 aact:AA01213|5end_competence_related_protein_829867..830097_POSITIVE 306 200 155 146 95 aact:AA02242|5end_molybdenum_cofactor_biosynthesis_protein_A_1523998..1524228_POSITIVE 305 57 13 162 124 aact:AA01298|5end_competence_protein_D_880554..880784_POSITIVE 304 188 142 108 50 aact:AA02077|5end_probable_outer_membrane_protein_1411294..1411524_POSITIVE 304 195 155 124 81 aact:AA00809|5end_possible_oxidoreductase_553211..553441_POSITIVE 303 190 141 133 86 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 303 194 151 95 49 aact:AA00733|5end_hypothetical_protein_502335..502565_POSITIVE 303 59 14 200 156 aact:AA00723|5end_peptidase_B_(leucyl_aminopeptidase)_496457..496687_POSITIVE 298 122 85 40 1 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 296 50 5 126 80 aact:AA00458|5end_nickel-binding_periplasmic_protein_319374..319604_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 339 200 155 90 45 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 330 49 4 136 86 aact:AA01519.1|3end_conserved_hypothetical_protein_1022920..1023070_POSITIVE 330 49 4 103 53 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE 319 160 112 133 73 aact:AA02657|3end_conserved_hypothetical_protein_1852340..1852490_POSITIVE 312 50 3 150 103 aact:AA02005|3end_vancomycin_sensitivity;_SanA_protein_1364020..1364170_POSITIVE 308 116 77 108 60 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 306 200 155 130 79 aact:AA02241|3end_molybdenum_cofactor_biosynthesis_protein_C_1524062..1524212_POSITIVE 306 59 17 138 87 aact:AA00618|3end_molybdopterin_biosynthesis_protein_423642..423792_POSITIVE 305 57 13 139 101 aact:AA01297|3end_competence_protein_E_precursor_880611..880761_POSITIVE 303 190 141 146 99 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 303 194 151 107 61 aact:AA00732|3end_citrate_lyase_ligase_502427..502577_POSITIVE 298 122 85 151 112 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 296 50 5 57 11 aact:AA00455|3end_lipoprotein_precursor_319385..319535_POSITIVE 292 193 153 53 12 aact:AA00929|3end_DNA_gyrase_subunit_B_640294..640444_POSITIVE 292 193 153 42 4 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 289 117 72 99 52 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 288 70 22 86 26 aact:AA01232|3end_30S_ribosomal_protein_S8_840914..841064_POSITIVE 283 45 4 69 33 aact:AA01177|3end_beta-ketoacyl-ACP_synthase_IV_808053..808203_POSITIVE 282 80 31 77 15 aact:AA01323|3end_50S_ribosomal_protein_L28_894560..894710_POSITIVE 281 190 141 72 18 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE