Origin IGS: tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgaccccttttcgggttttgcattgactggaaaacaatttacttttttctgtgatcctgataacttaccgcttgagatttgtacaattttataatttatactccatttcactttattcttaaggggtccca Mask Tandem Repeat Region ================================================ tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Find is-nt database================================================ Query_seq: AA00049:AA00050|AA00049:AA00050:conserved hypothetical protein:cytochrome c-type biogenesis protein precursor (reductase):->->:39925..40058 134 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00049:AA00050|AA00049:AA00050:conserved hypothetical protein:cytochrome c-type biogenesis protein precursor (reductase):->->:39925..40058 134 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00049:AA00050|AA00049:AA00050:conserved hypothetical protein:cytochrome c-type biogenesis protein precursor (reductase):->->:39925..40058 134 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 21 End: 134 .................... M K W S I N Y K I V Q I S S G K L S G S Q K K V N C F P V N A K P E K G S L Protein_Len: 31 Strand: - Start: 13 End: 105 ............ S Y L S I S Y L N Y F Q V F R L R Y T I L I V S F L L N N E M ............................. tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00049:AA00050|AA00049:AA00050:conserved hypothetical protein:cytochrome c-type biogenesis protein precursor (reductase):->->:39925..40058 134 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Intra-Species Hit: Count: 1 Min: 1 Max: 134 Len: 134 Subject: aact_AA00049_AA00050|conserved hypothetical protein:cytochrome c-type biogenesis protein precursor (reductase)|POSITIVE:POSITIVE|[39925,40058]|134 HSP 1 e-value: 2.0E-70 bit: 266.0 Len: 134 Query Start:1 Query End:134 Subject Strand: POSITIVE Subject Start: 1 Subject End: 134 tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta tgggaccccttaagaataaagtgaaatggagtataaattataaaattgtacaaatctcaagcggtaagttatcaggatcacagaaaaaagtaaattgttttccagtcaatgcaaaacccgaaaaggggtcatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGGACCCCUUAAGAAUAAAGUGAAAUGGAGUAUAAAUUAUAAAAUUGUACAAAUCUCAAGCGGUAAGUUAUCAGGAUCACAGAAAAAAGUAAAUUGUUUUCCAGUCAAUGCAAAACCCGAAAAGGGGUCAUUA ...((((((((.........((((..(((.((((((.........))))))........(((.....))).)))...))))...............(((((.((.....)).))))).....)))))))).... (-25.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGACCCCUUUUCGGGUUUUGCAUUGACUGGAAAACAAUUUACUUUUUUCUGUGAUCCUGAUAACUUACCGCUUGAGAUUUGUACAAUUUUAUAAUUUAUACUCCAUUUCACUUUAUUCUUAAGGGGUCCCA ....((((((((.(((((..(..((..((..(((((....))).))..))..))..).)))))............((((((..(((.((((....))))..)))...))))))..........))))))))... (-25.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 250 102 61 188 153 aact:AA00041|5end_cytochrome_c-type_biogenesis_protein_31881..32111_POSITIVE 240 100 59 79 29 aact:AA00263|5end_ATP-dependent_RNA_helicase_183690..183920_POSITIVE 238 129 82 59 18 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 228 119 73 214 162 aact:AA01746|5end_thiol:disulfide_interchange_protein_1180636..1180866_POSITIVE 225 94 56 175 136 aact:AA02929|5end_hypothetical_protein_2049189..2049419_POSITIVE 225 79 43 161 124 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 220 127 102 34 8 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 218 84 41 172 122 aact:AA01484|5end_ATP_synthase_F1,_subunit_gamma_997675..997905_POSITIVE 218 83 51 159 120 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 209 67 21 135 91 aact:AA01739|5end_conserved_hypothetical_protein_1175578..1175808_POSITIVE 208 90 57 54 16 aact:AA02074|5end_hypothetical_protein_1409340..1409570_POSITIVE 205 97 61 98 62 aact:AA02004|5end_homoserine_O-acetyltransferase_1361937..1362167_POSITIVE 205 83 60 67 42 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 204 120 74 198 149 aact:AA02126|5end_conserved_hypothetical_protein_1450471..1450701_POSITIVE 204 84 46 222 174 aact:AA00537|5end_conserved_hypothetical_protein_367992..368222_POSITIVE 203 128 97 222 199 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 202 107 61 196 147 aact:AA01092|5end_hypothetical_protein_756114..756344_POSITIVE 201 107 61 81 30 aact:AA02609|5end_ABC_transporter,_ATP-binding_protein/permease_1818446..1818676_POSITIVE 201 130 88 74 35 aact:AA01600|5end_phosphotransferase_system_enzyme_I_1080481..1080711_POSITIVE 200 103 61 229 187 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 238 129 82 84 43 aact:AA01315|3end_DNA/pantothenate_metabolism_flavoprotein_891367..891517_POSITIVE 228 119 73 130 78 aact:AA01745|3end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180632..1180782_POSITIVE 225 94 56 150 111 aact:AA02928|3end_conserved_hypothetical_protein_2049244..2049394_POSITIVE 225 79 43 127 90 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE 216 82 41 149 102 aact:AA01482|3end_ATP_synthase_F1,_subunit_beta_997735..997885_POSITIVE 216 39 1 86 52 aact:AA00565|3end_30S_ribosomal_protein_S7_390519..390669_POSITIVE 205 83 60 77 52 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 204 120 74 68 19 aact:AA02125|3end_conserved_hypothetical_protein;_possible_universal_stress_protein_1450421..1450571_POSITIVE 202 67 27 41 2 aact:AA01738|3end_enoyl-(acyl-carrier-protein)_reductase_1175564..1175714_POSITIVE 201 130 90 70 37 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 200 129 95 125 98 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 198 129 97 44 20 aact:AA01088|3end_rare_lipoprotein_B_precursor_753483..753633_POSITIVE 198 112 71 151 98 aact:AA00632|3end_inner_membrane_protein_ImpA_434653..434803_POSITIVE 198 83 51 147 114 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 196 130 99 116 80 aact:AA02375|3end_D-3-phosphoglycerate_dehydrogenase_1627742..1627892_POSITIVE 196 129 88 62 16 aact:AA01976|3end_colicin_V_production_protein_1337132..1337282_POSITIVE 195 121 82 123 75 aact:AA01011|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_707769..707919_POSITIVE 194 130 96 147 118 aact:AA00778|3end_trp_operon_repressor_536953..537103_POSITIVE 190 129 103 151 126 aact:AA01814|3end_endonuclease_III_1222593..1222743_POSITIVE 188 85 55 87 48 aact:AA02309|3end_glyceraldehyde_3-phosphate_dehydrogenase_1579229..1579379_POSITIVE