Origin IGS: taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggttagttttttattattgataaaggcgtataatgtacgctttttgtttagctgtttctactttcttgcttgtgcaagaaagtagcaaagaacacaccccagcttcaccgctgatcttcgcttggttgcaatttttttaacggaaatttttgaactcgccactacgcagctcaaacaaagcgaaaatttcctaaaaatta Mask Tandem Repeat Region ================================================ taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Find is-nt database================================================ Query_seq: AA00042:AA00043|AA00042:AA00043:hypothetical protein:formate dependent nitrite reductase, transmembrane protein:->->:34316..34517 202 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00042:AA00043|AA00042:AA00043:hypothetical protein:formate dependent nitrite reductase, transmembrane protein:->->:34316..34517 202 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00042:AA00043|AA00042:AA00043:hypothetical protein:formate dependent nitrite reductase, transmembrane protein:->->:34316..34517 202 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: + Start: 3 End: 140 .. M F R K F S L C L S C V V A S S K I S V K K I A T K R R S A V K L G C V L C Y F L A Q A R K .............................................................. Protein_Len: 33 Strand: + Start: 86 End: 184 ..................................................................................... M S G E A G V C S L L L S C T S K K V E T A K Q K A Y I I R L Y Q .................. Protein_Len: 36 Strand: - Start: 64 End: 171 ............................................................... F F Q L W A F I L P S A P T H E K S S E Q V L L F T S V A L C F A Y M M ............................... taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00042:AA00043|AA00042:AA00043:hypothetical protein:formate dependent nitrite reductase, transmembrane protein:->->:34316..34517 202 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 202 Len: 202 Subject: aact_AA00042_AA00043|hypothetical protein:formate dependent nitrite reductase, transmembrane protein|POSITIVE:POSITIVE|[34316,34517]|202 HSP 1 e-value: 2.0E-98 bit: 359.0 Len: 202 Query Start:1 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 202 taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttnnnnnnnttgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta taatttttaggaaattttcgctttgtttgagctgcgtagtggcgagttcaaaaatttccgttaaaaaaattgcaaccaagcgaagatcagcggtgaagctggggtgtgttctttgctactttcttgcacaagcaagaaagtagaaacagctaaacaaaaagcgtacattatacgcctttatcaataataaaaaactaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUUUAGGAAAUUUUCGCUUUGUUUGAGCUGCGUAGUGGCGAGUUCAAAAAUUUCCGUUAAAAAAAUUGCAACCAAGCGAAGAUCAGCGGUGAAGCUGGGGUGUGUUCUUUGCUACUUUCUUGCACAAGCAAGAAAGUAGAAACAGCUAAACAAAAAGCGUACAUUAUACGCCUUUAUCAAUAAUAAAAAACUAACCUA .......((((..((((((((((..((((((((.(((....))))))))))).......((..........))....))))))))))((((......)))).(((((((......((((((((((((....)))))))))))).....(((........))).......)))))))......................)))) (-56.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUAGUUUUUUAUUAUUGAUAAAGGCGUAUAAUGUACGCUUUUUGUUUAGCUGUUUCUACUUUCUUGCUUGUGCAAGAAAGUAGCAAAGAACACACCCCAGCUUCACCGCUGAUCUUCGCUUGGUUGCAAUUUUUUUAACGGAAAUUUUUGAACUCGCCACUACGCAGCUCAAACAAAGCGAAAAUUUCCUAAAAAUUA ((((..(((((((............((((((((...))))))))(((((((((((((..((((((((((((....))))))))))))((((((........((((......))))......((......)).................)))))).............)))))).)))))))..))))))).))))....... (-53.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 794 120 71 51 2 aact:AA00237|5end_hypothetical_protein_170934..171164_POSITIVE 651 60 11 144 95 aact:AA02489|5end_hypothetical_protein_1726454..1726684_POSITIVE 369 143 101 60 5 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 364 106 69 228 187 aact:AA02439|5end_integral_membrane_protein;_possible_ABC_transporter_permease_1683902..1684132_POSITIVE 358 130 89 169 126 aact:AA00925|5end_hypothetical_protein_635364..635594_POSITIVE 358 130 89 66 23 aact:AA00924|5end_hypothetical_protein_635261..635491_POSITIVE 357 135 91 214 156 aact:AA02384|5end_2-dehydro-3-deoxygluconokinase_1632112..1632342_POSITIVE 341 137 91 68 2 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 337 60 14 215 162 aact:AA01577|5end_conserved_hypothetical_transmembrane_protein_1063084..1063314_POSITIVE 330 117 72 52 13 aact:AA00374|5end_short-chain_fatty_acids_transporter_251681..251911_POSITIVE 328 110 70 53 10 aact:AA01952|5end_ABC_transporter,_substrate_binding_protein_1318987..1319217_POSITIVE 325 116 72 224 182 aact:AA02337|5end_DNA_mismatch_repair_protein_1597350..1597580_POSITIVE 325 107 67 104 59 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 324 108 72 77 33 aact:AA01772|5end_shikimate_5-dehydrogenase_protein_1194050..1194280_POSITIVE 323 110 70 84 37 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 320 109 71 194 149 aact:AA02585|5end_hypothetical_protein_1798798..1799028_POSITIVE 320 117 76 176 114 aact:AA00291|5end_hypothetical_protein_201715..201945_POSITIVE 315 48 9 41 3 aact:AA00471|5end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328262..328492_POSITIVE 313 119 76 168 102 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 308 140 92 131 73 aact:AA01496|5end_glucose_inhibited_division_protein_A_1005125..1005355_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 794 120 71 58 9 aact:AA00236|3end_anaerobic_ribonucleoside-triphosphate_reductase_171021..171171_POSITIVE 651 60 11 128 79 aact:AA02487|3end_hypothetical_protein_1726518..1726668_POSITIVE 464 60 11 146 104 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 364 140 91 141 84 aact:AA02727|3end_hypothetical_protein_1909925..1910075_POSITIVE 358 130 89 45 2 aact:AA00923|3end_diaminopimelate_decarboxylase_635320..635470_POSITIVE 338 140 91 109 52 aact:AA02734|3end_leucine-responsive_regulatory_protein_1915692..1915842_POSITIVE 330 117 72 46 7 aact:AA00373|3end_CoA-transferase_(beta)_251755..251905_POSITIVE 311 49 7 141 88 aact:AA00709|3end_metallocluster_assembly_(NifU_protein)_487838..487988_POSITIVE 305 179 146 46 13 aact:AA01903|3end_hypothetical_protein_1284215..1284365_POSITIVE 303 109 72 45 1 aact:AA02584|3end_ADP-heptose_synthase_1798729..1798879_POSITIVE 300 116 72 148 105 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE 294 117 76 149 96 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 292 60 15 124 65 aact:AA02481|3end_conserved_hypothetical_protein_1720103..1720253_POSITIVE 292 118 71 67 12 aact:AA01033|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_723435..723585_POSITIVE 287 118 72 109 57 aact:AA00550|3end_ferredoxin-type_protein_377201..377351_POSITIVE 286 107 63 73 17 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 284 116 75 94 40 aact:AA00245|3end_hypothetical_protein_173192..173342_POSITIVE 283 60 13 134 90 aact:AA03008|3end_GTP-binding_protein_era_homolog_(GTPase)_2101072..2101222_POSITIVE 283 46 6 141 103 aact:AA00866|3end_conserved_hypothetical_protein_600250..600400_POSITIVE 281 106 65 133 87 aact:AA00473|3end_tryptophanyl-tRNA_synthetase_330077..330227_POSITIVE