Origin IGS: aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaataaggtattagcctatttttttactatttaaaaatactatttaaaggcaagaaccatgcaaatagctttcaatttattctaaaacataccaatagcagtcaaattattt Mask Tandem Repeat Region ================================================ aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Find is-nt database================================================ Query_seq: AA00037:AA00038|AA00037:AA00038:conserved hypothetical protein:conserved hypothetical protein:->->:29967..30080 114 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00037:AA00038|AA00037:AA00038:conserved hypothetical protein:conserved hypothetical protein:->->:29967..30080 114 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00037:AA00038|AA00037:AA00038:conserved hypothetical protein:conserved hypothetical protein:->->:29967..30080 114 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: - Start: 2 End: 103 . I I Q S S N T H K L I F Q F S N A H N K G K F L I K L Y Y F F L S M ........... aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00037:AA00038|AA00037:AA00038:conserved hypothetical protein:conserved hypothetical protein:->->:29967..30080 114 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA00037_AA00038|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[29967,30080]|114 HSP 1 e-value: 4.0E-46 bit: 184.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtnnnnnnntaggctaataccttatttta aaataatttgactgctattggtatgttttagaataaattgaaagctatttgcatggttcttgcctttaaatagtatttttaaatagtaaaaaaataggctaataccttatttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAAUUUGACUGCUAUUGGUAUGUUUUAGAAUAAAUUGAAAGCUAUUUGCAUGGUUCUUGCCUUUAAAUAGUAUUUUUAAAUAGUAAAAAAAUAGGCUAAUACCUUAUUUUA ...............(((((((.((((((........((((((((((((((...(((....)))..))))))))..))))))........)))))).))))))).......... (-17.69) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUAAGGUAUUAGCCUAUUUUUUUACUAUUUAAAAAUACUAUUUAAAGGCAAGAACCAUGCAAAUAGCUUUCAAUUUAUUCUAAAACAUACCAAUAGCAGUCAAAUUAUUU ........(((((..((((((((((.........)))))).........))))..(((....((.....)).)))...............)))))................... (-10.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 69 21 137 86 aact:AA00745|5end_galactokinase_511163..511393_POSITIVE 228 57 16 59 13 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 220 59 11 160 112 aact:AA02159|5end_conserved_hypothetical_protein_1477333..1477563_POSITIVE 206 52 11 150 96 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 205 65 23 199 157 aact:AA02763|5end_DNA_polymerase_III,_epsilon_subunit_1933696..1933926_POSITIVE 205 56 14 59 23 aact:AA00814|5end_conserved_hypothetical_protein_559125..559355_POSITIVE 204 75 44 115 82 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 204 63 42 231 208 aact:AA00165|5end_conserved_hypothetical_protein_117358..117588_POSITIVE 203 69 37 66 18 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 202 64 30 109 71 aact:AA02296|5end_conserved_hypothetical_protein_(possible_cell_wall-associated_hydrolase)_1566070..1566300_POSITIVE 202 64 30 109 71 aact:AA00620|5end_hydrolase_425182..425412_POSITIVE 202 64 30 109 71 aact:AA00411|5end_hydrolase_275798..276028_POSITIVE 198 74 37 205 157 aact:AA00479|5end_mannose-specific_phosphotransferase_system_IIC_333475..333705_POSITIVE 196 73 40 124 81 aact:AA02088|5end_glycogen_operon_protein_1421282..1421512_POSITIVE 195 63 21 174 128 aact:AA01199|5end_conserved_hypothetical_protein_821984..822214_POSITIVE 194 68 44 108 82 aact:AA02151|5end_ferric_enterobactin_receptor;_TonB-dependent_outer_membrane_siderophore_receptor_protein_1470856..1471086_POSITIVE 193 56 14 80 41 aact:AA02642|5end_ABC_transporter,_ATP-binding_protein_1839357..1839587_POSITIVE 192 65 31 153 111 aact:AA02830|5end_colicin_tolerance_protein_1988729..1988959_POSITIVE 189 59 11 54 2 aact:AA02803|5end_leukotoxin_secretion_protein_D;_hemolysin_export_system_membrane_fusion_protein_1970282..1970512_POSITIVE 189 64 35 42 19 aact:AA02254|5end_2-oxoglutarate/malate_translocator_1534846..1535076_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 256 69 21 140 89 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 228 57 16 59 13 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 220 59 11 64 16 aact:AA02158|3end_TonB-dependent_outer_membrane_receptor_1477317..1477467_POSITIVE 209 59 11 130 79 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 208 56 13 78 23 aact:AA01782|3end_molybdenum_transport_protein;_transcriptional_regulator_1203735..1203885_POSITIVE 205 65 23 131 89 aact:AA02762|3end_ribonuclease_H_1933708..1933858_POSITIVE 199 78 37 90 43 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 194 66 34 143 117 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 193 56 14 80 41 aact:AA02640|3end_rhamnosyltrasferase_1839437..1839587_POSITIVE 192 65 31 135 93 aact:AA02829|3end_peptidoglycan-associated_outer_membrane_1988791..1988941_POSITIVE 184 64 22 61 14 aact:AA01644|3end_ferric_transport_system_permease_protein_1114160..1114310_POSITIVE 184 50 6 127 76 aact:AA01333|3end_conserved_hypothetical_protein;_possible_capsular_polysaccharide_synthesis_protein_900813..900963_POSITIVE 184 80 37 80 19 aact:AA00325|3end_conserved_hypothetical_protein_221720..221870_POSITIVE 180 80 43 100 72 aact:AA01228|3end_50S_ribosomal_protein_L14_839288..839438_POSITIVE 179 64 42 75 47 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 178 59 11 84 31 aact:AA01340|3end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_907019..907169_POSITIVE 177 59 13 74 31 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE 177 106 85 55 37 aact:AA01164|3end_conserved_hypothetical_protein_(possible_integral_membrane_protein)_798148..798298_POSITIVE 177 59 37 140 120 aact:AA00075|3end_conserved_hypothetical_protein_55636..55786_POSITIVE 173 71 33 95 53 aact:AA01631|3end_dihydrodipicolinate_reductase_1103915..1104065_POSITIVE