Origin IGS: ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataccgtgataagacgaaggattatttccttccacaagacatctatctgcgtataaaaccgcagttttaacgagaaaaaatgttatttttgcgatctcactcgaaaaattgaaacacatttctcgacagaaatcacttaactgttaaagtgtcgtccagttatcaacctatcaactaactggagaaaacc Mask Tandem Repeat Region ================================================ ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Find is-nt database================================================ Query_seq: AA00030:AA00031|AA00030:AA00031:hypothetical protein:conserved hypothetical protein:->->:25229..25420 192 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00030:AA00031|AA00030:AA00031:hypothetical protein:conserved hypothetical protein:->->:25229..25420 192 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00030:AA00031|AA00030:AA00031:hypothetical protein:conserved hypothetical protein:->->:25229..25420 192 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 72 End: 191 ....................................................................... M C F N F S S E I A K I T F F L V K T A V L Y A D R C L V E G N N P S S Y H G I . ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -3.1 Tail_Score: -3.23219 Start: 158 End: 176 Full_Region: AGATAGATGTCTTGT GGAAGGA AATAA TCCTTCG TCTTATCACGGTATT .............................................................................................................................................................GGAAGGAAATAATCCTTCG................ TransTerm Strand: + Conf: 57 HP_score: -6.6 Tail_Score: -2.53449 Start: 159 End: 175 Full_Region: GATAGATGTCTTGTG GAAGGA AATAA TCCTTC GTCTTATCACGGTAT ..............................................................................................................................................................GAAGGAAATAATCCTTC................. Find igs database================================================ Query_seq: AA00030:AA00031|AA00030:AA00031:hypothetical protein:conserved hypothetical protein:->->:25229..25420 192 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Intra-Species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: aact_AA00030_AA00031|hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[25229,25420]|192 HSP 1 e-value: 1.0E-105 bit: 381.0 Len: 192 Query Start:1 Query End:192 Subject Strand: POSITIVE Subject Start: 1 Subject End: 192 ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta ggttttctccagttagttgataggttgataactggacgacactttaacagttaagtgatttctgtcgagaaatgtgtttcaatttttcgagtgagatcgcaaaaataacattttttctcgttaaaactgcggttttatacgcagatagatgtcttgtggaaggaaataatccttcgtcttatcacggtatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUCUCCAGUUAGUUGAUAGGUUGAUAACUGGACGACACUUUAACAGUUAAGUGAUUUCUGUCGAGAAAUGUGUUUCAAUUUUUCGAGUGAGAUCGCAAAAAUAACAUUUUUUCUCGUUAAAACUGCGGUUUUAUACGCAGAUAGAUGUCUUGUGGAAGGAAAUAAUCCUUCGUCUUAUCACGGUAUUA (((.((.((((((((.(..(....)..))))))))).)).)))...((((....((((((((..(((((((((........)))))))))..)))))))).......((((((..((((((((((((....)))))).))).))).)))))).)))).((((((.....))))))................. (-51.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUACCGUGAUAAGACGAAGGAUUAUUUCCUUCCACAAGACAUCUAUCUGCGUAUAAAACCGCAGUUUUAACGAGAAAAAAUGUUAUUUUUGCGAUCUCACUCGAAAAAUUGAAACACAUUUCUCGACAGAAAUCACUUAACUGUUAAAGUGUCGUCCAGUUAUCAACCUAUCAACUAACUGGAGAAAACC .......(((((.....((((((.....)))))).........(((..(((((........)))))......(((((((...((((((((((.(((......)))))))))...))))..)))))))..))).)))))...(((.....))).((.((((((((.............))))))))))..... (-42.12) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 167 129 217 178 aact:AA00013|5end_hypothetical_protein_6695..6925_POSITIVE 290 160 118 183 141 aact:AA00686|5end_hypothetical_protein_468852..469082_POSITIVE 290 160 119 145 100 aact:AA00595|5end_hypothetical_protein_407194..407424_POSITIVE 288 160 119 158 118 aact:AA02253|5end_hypothetical_protein_1534605..1534835_POSITIVE 286 70 22 200 149 aact:AA02086|5end_4-alpha-glucanotransferase_1418377..1418607_POSITIVE 283 163 129 54 12 aact:AA01718|5end_DNA_polymerase_III,_chi_subunit_1162790..1163020_POSITIVE 274 45 1 66 11 aact:AA01086|5end_hypothetical_protein_751392..751622_POSITIVE 271 102 61 150 99 aact:AA01651|5end_hypothetical_protein_1119796..1120026_POSITIVE 267 112 77 55 20 aact:AA00562|5end_hypothetical_protein_385258..385488_POSITIVE 267 166 123 162 117 aact:AA00453|5end_phosphoglyceromutase_317249..317479_POSITIVE 264 67 23 70 17 aact:AA01815|5end_probable_sodium-dependent_transmembrane_transport_protein_1222541..1222771_POSITIVE 258 178 133 212 166 aact:AA00985|5end_transcriptional_regulator_(LysR_family)_688771..689001_POSITIVE 257 169 129 180 143 aact:AA00909.1|5end_hypothetical_protein_625970..626200_POSITIVE 254 31 1 72 44 aact:AA02610|5end_acyl-CoA_thioesterase_II_1820371..1820601_POSITIVE 254 99 58 113 81 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 254 179 134 149 102 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 254 98 54 141 93 aact:AA00058|5end_cytochrome_c-type_biogenesis_protein_44664..44894_POSITIVE 252 163 121 155 111 aact:AA02344|5end_GTP-binding_protein,_elongation_factor_typA/bipA_1601966..1602196_POSITIVE 251 100 53 98 55 aact:AA00303|5end_thymidylate_kinase_(DTMP)_206107..206337_POSITIVE 249 189 141 191 138 aact:AA02886|5end_OBG-related_GTP-binding_protein_2023382..2023612_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 301 164 121 100 65 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 290 160 118 112 70 aact:AA00684|3end_adenylosuccinate_synthase_468861..469011_POSITIVE 286 70 22 97 46 aact:AA02084|3end_conserved_hypothetical_protein_1418354..1418504_POSITIVE 271 102 61 123 72 aact:AA01650|3end_maltose_transport_inner_membrane_protein_1119849..1119999_POSITIVE 255 159 119 56 8 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 254 99 58 129 97 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 254 98 54 138 90 aact:AA00057|3end_cytochrome_c-type_biogenesis_protein_44741..44891_POSITIVE 251 100 53 116 73 aact:AA00302|3end_DNA_polymerase_III,_delta'_subunit_206205..206355_POSITIVE 249 189 141 151 98 aact:AA02885|3end_conserved_hypothetical_protein_2023422..2023572_POSITIVE 248 176 140 129 96 aact:AA01464|3end_hypothetical_protein_984403..984553_POSITIVE 246 187 141 124 75 aact:AA00196|3end_conserved_hypothetical_protein_138015..138165_POSITIVE 244 100 60 131 96 aact:AA01644|3end_ferric_transport_system_permease_protein_1114160..1114310_POSITIVE 241 158 119 151 112 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 237 100 58 134 95 aact:AA02330|3end_UDP-N-acetylglucosamine_enolpyruvyl_transferase_1591281..1591431_POSITIVE 236 189 141 42 1 aact:AA01930|3end_ribosome-binding_factor_A_1305175..1305325_POSITIVE 235 100 55 65 13 aact:AA00482|3end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_336484..336634_POSITIVE 234 105 61 111 68 aact:AA01853|3end_hypothetical_protein_1246645..1246795_POSITIVE 233 134 91 64 14 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 229 184 144 51 8 aact:AA02594|3end_conserved_hypothetical_protein_1805124..1805274_POSITIVE 229 134 96 70 24 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE