Origin IGS: tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttaattacatcagcaaacagaactatcatctacggactttaagtaccattttgggtggtatgttgagacaaaacaaacacttaagccaatagcattaagggcaatcttagatagttaagattgcccttaatgttcaaggaataaatggtataaagcatttttataccttatattgatgtattatattttgtgctatgaatcta Mask Tandem Repeat Region ================================================ tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Find is-nt database================================================ Query_seq: AA00022:AA00024|AA00022:AA00024:conserved hypothetical protein:transcriptional regulatory protein:->->:18945..19150 206 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00022:AA00024|AA00022:AA00024:conserved hypothetical protein:transcriptional regulatory protein:->->:18945..19150 206 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00022:AA00024|AA00022:AA00024:conserved hypothetical protein:transcriptional regulatory protein:->->:18945..19150 206 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 99 End: 191 .................................................................................................. M A L N A I G L S V C F V S T Y H P K W Y L K S V D D S S V C ............... tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 45 HP_score: -2.7 Tail_Score: -3.35867 Start: 32 End: 62 Full_Region: AATATAATACATCAA TATAAGGTATAAA AATGC TTTATACCATTTA TTCCTTGAACATTAA ...............................TATAAGGTATAAAAATGCTTTATACCATTTA................................................................................................................................................ TransTerm Strand: - Conf: 74 HP_score: -7.7 Tail_Score: -3.75655 Start: 37 End: 57 Full_Region: gttcaaggaataaat ggtataaag cat ttttatacc ttatattgatgtatt ....................................ggtataaagcatttttatacc..................................................................................................................................................... TransTerm Strand: + Conf: 69 HP_score: -7.7 Tail_Score: -3.31416 Start: 64 End: 119 Full_Region: CTTTATACCATTTAT TCCTTGAACATTAAGGGCAATCTTA ACTATC TAAGATTGCCCTTAATGCTATTGGC TTAAGTGTTTGTTTT ...............................................................TCCTTGAACATTAAGGGCAATCTTAACTATCTAAGATTGCCCTTAATGCTATTGGC....................................................................................... TransTerm Strand: + Conf: 100 HP_score: -13.6 Tail_Score: -3.2128 Start: 69 End: 114 Full_Region: TACCATTTATTCCTT GAACATTAAGGGCAATCTTA ACTATC TAAGATTGCCCTTAATGCTA TTGGCTTAAGTGTTT ....................................................................GAACATTAAGGGCAATCTTAACTATCTAAGATTGCCCTTAATGCTA............................................................................................ TransTerm Strand: + Conf: 100 HP_score: -16.2 Tail_Score: -3.16117 Start: 71 End: 112 Full_Region: CCATTTATTCCTTGA ACATTAAGGGCAATCTTA ACTATC TAAGATTGCCCTTAATGC TATTGGCTTAAGTGT ......................................................................ACATTAAGGGCAATCTTAACTATCTAAGATTGCCCTTAATGC.............................................................................................. TransTerm Strand: + Conf: 100 HP_score: -16.5 Tail_Score: -2.77791 Start: 74 End: 109 Full_Region: TTTATTCCTTGAACA TTAAGGGCAATCTTA ACTATC TAAGATTGCCCTTAA TGCTATTGGCTTAAG .........................................................................TTAAGGGCAATCTTAACTATCTAAGATTGCCCTTAA................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -17.2 Tail_Score: -3.02511 Start: 74 End: 109 Full_Region: cttaagccaatagca ttaagggcaatcttag atag ttaagattgcccttaa tgttcaaggaataaa .........................................................................ttaagggcaatcttagatagttaagattgcccttaa................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -12.9 Tail_Score: -3.2819 Start: 78 End: 105 Full_Region: TTCCTTGAACATTAA GGGCAATCTTA ACTATC TAAGATTGCCC TTAATGCTATTGGCT .............................................................................GGGCAATCTTAACTATCTAAGATTGCCC..................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.6 Tail_Score: -3.36273 Start: 78 End: 105 Full_Region: agccaatagcattaa gggcaatcttag atag ttaagattgccc ttaatgttcaaggaa .............................................................................gggcaatcttagatagttaagattgccc..................................................................................................... Find igs database================================================ Query_seq: AA00022:AA00024|AA00022:AA00024:conserved hypothetical protein:transcriptional regulatory protein:->->:18945..19150 206 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 206 Len: 206 Subject: aact_AA00022_AA00024|conserved hypothetical protein:transcriptional regulatory protein|POSITIVE:POSITIVE|[18945,19150]|206 HSP 1 e-value: 1.0E-113 bit: 408.0 Len: 206 Query Start:1 Query End:206 Subject Strand: POSITIVE Subject Start: 1 Subject End: 206 tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa tagattcatagcacaaaatataatacatcaatataaggtataaaaatgctttataccatttattccttgaacattaagggcaatcttaactatctaagattgcccttaatgctattggcttaagtgtttgttttgtctcaacataccacccaaaatggtacttaaagtccgtagatgatagttctgtttgctgatgtaattaaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUUCAUAGCACAAAAUAUAAUACAUCAAUAUAAGGUAUAAAAAUGCUUUAUACCAUUUAUUCCUUGAACAUUAAGGGCAAUCUUAACUAUCUAAGAUUGCCCUUAAUGCUAUUGGCUUAAGUGUUUGUUUUGUCUCAACAUACCACCCAAAAUGGUACUUAAAGUCCGUAGAUGAUAGUUCUGUUUGCUGAUGUAAUUAAAAA .........((.((((((((...........((((((((((....)))))))))).((((((..((.....(((((((((((((((((......))))))))))))))))).....))..))))))..)))))))))).....(((((.......)))))......(((.((((((((....)).)))))).)))........... (-54.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUAAUUACAUCAGCAAACAGAACUAUCAUCUACGGACUUUAAGUACCAUUUUGGGUGGUAUGUUGAGACAAAACAAACACUUAAGCCAAUAGCAUUAAGGGCAAUCUUAGAUAGUUAAGAUUGCCCUUAAUGUUCAAGGAAUAAAUGGUAUAAAGCAUUUUUAUACCUUAUAUUGAUGUAUUAUAUUUUGUGCUAUGAAUCUA ..........(((.(((..((((((......((....))......(((((((((((((((...((((.......))))..))))))..((...((((((((((((((((((((....))))))))))))))))))))...))...)))))))))...(((((..((((...))))..)))))......))))))))))))...... (-57.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 196 156 141 99 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 255 139 95 177 120 aact:AA01722|5end_conserved_hypothetical_protein_1163434..1163664_POSITIVE 255 147 101 150 105 aact:AA00227|5end_hypothetical_sequence_156407..156637_POSITIVE 252 137 94 195 150 aact:AA01581|5end_possible_GTP-binding_protein_1065265..1065495_POSITIVE 250 130 86 156 117 aact:AA01488|5end_ATP_synthase_F0,_subunit_b_1000676..1000906_POSITIVE 246 181 145 51 24 aact:AA02365|5end_hflC_protein_1621997..1622227_POSITIVE 243 126 81 50 7 aact:AA01538|5end_conserved_hypothetical_protein_1031527..1031757_POSITIVE 243 186 145 138 98 aact:AA01387|5end_primosomal_protein_N_936217..936447_POSITIVE 242 175 131 157 104 aact:AA02145|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1468602..1468832_POSITIVE 240 150 104 178 121 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 240 193 154 72 34 aact:AA01064|5end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_737428..737658_POSITIVE 238 120 78 224 179 aact:AA00342|5end_hypothetical_protein_229695..229925_POSITIVE 237 150 110 179 142 aact:AA00692|5end_conserved_hypothetical_protein_473236..473466_POSITIVE 237 197 163 114 61 aact:AA00539|5end_ADP-L-glycero-D-manno-heptose-6-epimerase_369044..369274_POSITIVE 236 175 145 52 22 aact:AA01799|5end_polysacharide_biosynthesis_protein_(Orf14_of_cluster)_1212459..1212689_POSITIVE 235 126 81 219 165 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 235 184 142 103 58 aact:AA00596|5end_uridylate_kinase_407496..407726_POSITIVE 232 122 82 230 193 aact:AA02292|5end_DNA-binding_protein_H-NS_1562922..1563152_POSITIVE 231 136 99 168 129 aact:AA00856|5end_arginine_ABC_transporter,_periplasmic-binding_protein_590251..590481_POSITIVE 229 160 111 60 3 aact:AA02687|5end_hydrogenase-4_component_D_1872521..1872751_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 196 156 150 108 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 256 117 79 51 4 aact:AA01596|3end_conserved_hypothetical_protein;_possible_membrane_protein_1077947..1078097_POSITIVE 255 139 95 146 89 aact:AA01720|3end_conserved_hypothetical_protein_1163483..1163633_POSITIVE 255 147 101 114 69 aact:AA00226|3end_hypothetical_protein_156451..156601_POSITIVE 254 118 73 149 109 aact:AA01695|3end_carbohydrate_transport_protein_1152294..1152444_POSITIVE 252 137 94 133 88 aact:AA01579|3end_transcriptional_regulatory_protein_1065283..1065433_POSITIVE 250 130 86 139 100 aact:AA01487|3end_ATP_synthase_F1,_subunit_delta_1000739..1000889_POSITIVE 249 126 95 34 3 aact:AA00022|3end_conserved_hypothetical_protein_18884..19034_POSITIVE 246 181 145 48 21 aact:AA02363|3end_hflK_protein_1622074..1622224_POSITIVE 243 126 81 50 7 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 243 186 145 122 82 aact:AA01386|3end_30S_ribosomal_protein_S1_936281..936431_POSITIVE 241 106 63 140 103 aact:AA02539|3end_transcriptional_activator_1766929..1767079_POSITIVE 240 150 104 144 87 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 240 193 154 41 3 aact:AA01063|3end_conserved_hypothetical_protein_737477..737627_POSITIVE 237 197 163 101 48 aact:AA00538|3end_hypothetical_protein_369111..369261_POSITIVE 235 130 93 136 87 aact:AA00075|3end_conserved_hypothetical_protein_55636..55786_POSITIVE 228 187 145 92 47 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 228 139 102 135 106 aact:AA01056|3end_conserved_hypothetical_protein_734168..734318_POSITIVE 226 172 131 150 101 aact:AA02144|3end_iron_(III)_ABC_transporter,_permease_protein_1468679..1468829_POSITIVE 225 118 79 113 83 aact:AA01140|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_782526..782676_POSITIVE