Origin IGS: tagaagggagctaccatgcgcgtaaatattacacttgaacacaaagaatctggtgaacgcttgtaccttacttctaaaaacaaacgcaacacaccagaccgtcttcaattgaagaaatactcaccaaaacttcgcaagcacgttgtgtttacagaagtgaagtaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaacacctcattcaaatatatttttgaatgtattctaacattaaactttgtagatgtcaacttcagctccatcaaaatatagataagaaggtagtgtaccaaatattaaaaagccctgccatcgaaattatagcagggcttaacttcaatatccagatgatatatttatctaaaaaaggtagagaaaaaggtagagtttgaattgaaatacagtgcaatatatcgcatttggcaaaaagaaaaaacgcttgaaatcagcgtttttcttctgtattgattcgtattgaatgcttacttcacttctgtaaacacaacgtgcttgcgaagttttggtgagtatttcttcaattgaagacggtctggtgtgttgcgtttgtttttagaagtaaggtacaagcgttcaccagattctttgtgttcaagtgtaatatttacgcgcatggtagctcccttcta Mask Tandem Repeat Region ================================================ tagaagggagctaccatgcgcgtaaatattacacttgaacacaaagaatctggtgaacgcttgtaccttacttctaaaaacaaacgcaacacaccagaccgtcttcaattgaagaaatactcaccaaaacttcgcaagcacgttgtgtttacagaagtgaagtaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Find is-nt database================================================ Query_seq: SSA_2287:SSA_2288|SSA_2287:SSA_2288:50S ribosomal protein L32, putative:Cadmium resistance transporter, putative:->->:2290144..2290600 457 tagaagggagctaccatgcgcgtaaatattacacttgaacacaaagaatctggtgaacgcttgtaccttacttctaaaaacaaacgcaacacaccagaccgtcttcaattgaagaaatactcaccaaaacttcgcaagcacgttgtgtttacagaagtgaagtaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_2287:SSA_2288|SSA_2287:SSA_2288:50S ribosomal protein L32, putative:Cadmium resistance transporter, putative:->->:2290144..2290600 457 tagaagggagctaccatgcgcgtaaatattacacttgaacacaaagaatctggtgaacgcttgtaccttacttctaaaaacaaacgcaacacaccagaccgtcttcaattgaagaaatactcaccaaaacttcgcaagcacgttgtgtttacagaagtgaagtaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 16 Max: 162 Len: 147 Subject: gi|89110375|ref|AP_004155.1| 50S ribosomal subunit protein L33 [Escherichia coli W3110] HSP 1 e-value: 9.0E-6 bit: 46.2 Len: 147 Query Start:16 Query End:162 Subject Strand: null Subject Start: 5 Subject End: 53 ............... M R V N I T L E H K E S G E R L Y L T S K N K R N T P D R L Q L K K Y S P K L R K H V V F T E V K ....................................................................................................................................................................................................................................................................................................... ............... I R E K I K L V S S A G T G H F Y T T T K N K R T K P E K L E L K K F D P V V R Q H V I Y K E A K ....................................................................................................................................................................................................................................................................................................... Find nr database================================================ Query_seq: SSA_2287:SSA_2288|SSA_2287:SSA_2288:50S ribosomal protein L32, putative:Cadmium resistance transporter, putative:->->:2290144..2290600 457 tagaagggagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaagggagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 16 End: 162 ............... M R V N I T L E H K E S G E R L Y L T S K N K R N T P D R L Q L K K Y S P K L R K H V V F T E V K ....................................................................................................................................................................................................................................................................................................... Protein_Len: 59 Strand: + Start: 108 End: 284 ........................................................................................................... M E E I L T K T S Q A R C V Y R S E V S I Q Y E S I Q K K N A D F K R F F F L P N A I Y C T V F Q F K L Y L F L Y L F ............................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 199 End: 288 ...................................................................................................................................................................................................... M I S S V F S F C Q M R Y I A L Y F N S N S T F F S T F F R ......................................................................................................................................................................... Protein_Len: 49 Strand: + Start: 236 End: 382 ........................................................................................................................................................................................................................................... M L H C I S I Q T L P F S L P F L D K Y I I W I L K L S P A I I S M A G L F N I W Y T T F L S I F ........................................................................... Protein_Len: 34 Strand: + Start: 355 End: 456 .................................................................................................................................................................................................................................................................................................................................................................. M V H Y L L I Y I L M E L K L T S T K F N V R I H S K I Y L N E V F . Protein_Len: 57 Strand: - Start: 259 End: 429 .................................................................................................................................................................................................................................................................. V R G K E R G K K S L Y I M Q I N F N L G A I I E I A P S K L I Q Y V V K K D I N Q H L Q L Q C R C L K I N S Y M ............................ Protein_Len: 37 Strand: - Start: 183 End: 293 ...................................................................................................................................................................................... Y L L F V S I E L T K E K Q W I R Y I A S Y K L E F E V K K E V K K L Y M .................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 3 End: 92 .. F P A V M R T F I V S S C L S D P S R K Y R V E L F L R L M ............................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 36 Strand: - Start: 1 End: 108 L L S S G H A Y I N C K F V F F R T F A Q V K S R F V F A V C W V T K M ............................................................................................................................................................................................................................................................................................................................................................. tagaagggagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 91 Start: 396 End: 425 ...........................................................................................................................................................................................................................................................................................................................................................................................................GTTGACATCTACAAAGTTTAATGTTAGAAT................................ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 301 End: 330 ............................................................................................................................................................................................................................................................................................................CTGGATATTGAAGTTAAGCCCTGCTATAAT............................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaagggagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -2.1 Tail_Score: -3.54424 Start: 133 End: 147 Full_Region: TACTCACCAAAACTT CGCAA GCACG TTGTG TTTACAGAAGTGAAG ....................................................................................................................................CGCAAGCACGTTGTG...................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 65 HP_score: -3.6 Tail_Score: -4.58395 Start: 191 End: 218 Full_Region: CGAATCAATACAGAA GAAAAACGCT GATTTCA AGCGTTTTTTC TTTTTGCCAAATGCG ..............................................................................................................................................................................................GAAAAACGCTGATTTCAAGCGTTTTTTC............................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 55 HP_score: -3.8 Tail_Score: -3.72909 Start: 191 End: 217 Full_Region: gcatttggcaaaaag aaaaaacgct tgaaatc agcgtttttc ttctgtattgattcg ..............................................................................................................................................................................................aaaaaacgcttgaaatcagcgtttttc................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 86 HP_score: -2.8 Tail_Score: -5.93252 Start: 197 End: 211 Full_Region: AATACAGAAGAAAAA CGCT GATTTCA AGCG TTTTTTCTTTTTGCC ....................................................................................................................................................................................................CGCTGATTTCAAGCG...................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 72 HP_score: -2.8 Tail_Score: -5.28561 Start: 197 End: 211 Full_Region: ggcaaaaagaaaaaa cgct tgaaatc agcg tttttcttctgtatt ....................................................................................................................................................................................................cgcttgaaatcagcg...................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -10.2 Tail_Score: -4.946 Start: 318 End: 344 Full_Region: GGATATTGAAGTTAA GCCCTGCTAT AATTTCG ATGGCAGGGC TTTTTAATATTTGGT .............................................................................................................................................................................................................................................................................................................................GCCCTGCTATAATTTCGATGGCAGGGC................................................................................................................. TransTerm Strand: - Conf: 70 HP_score: -8.0 Tail_Score: -3.20164 Start: 318 End: 344 Full_Region: accaaatattaaaaa gccctgccat cgaaatt atagcagggc ttaacttcaatatcc .............................................................................................................................................................................................................................................................................................................................gccctgccatcgaaattatagcagggc................................................................................................................. Find igs database================================================ Query_seq: SSA_2287:SSA_2288|SSA_2287:SSA_2288:50S ribosomal protein L32, putative:Cadmium resistance transporter, putative:->->:2290144..2290600 457 tagaagggagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntaagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Intra-Species Hit: Count: 1 Min: 163 Max: 457 Len: 295 Subject: NC_009009_SSA_2287_SSA_2288|50S ribosomal protein L32, putative:Cadmium resistance transporter, putative|POSITIVE:POSITIVE|[2290144,2290600]|457 HSP 1 e-value: 1.0E-144 bit: 513.0 Len: 295 Query Start:163 Query End:457 Subject Strand: POSITIVE Subject Start: 163 Subject End: 457 ..................................................................................................................................................................taagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgnnnnnnnnnnnngccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ..................................................................................................................................................................taagcattcaatacgaatcaatacagaagaaaaacgctgatttcaagcgttttttctttttgccaaatgcgatatattgcactgtatttcaattcaaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt Inter-species Hit: Count: 21 Min: 168 Max: 457 Len: 290 Subject: NC_004368_gbs2063_gbs2064|50S ribosomal protein L33:hypothetical protein|POSITIVE:POSITIVE|[2137910,2138200]|291 HSP 1 e-value: 2.0E-85 bit: 317.0 Len: 200 Query Start:258 Query End:457 Subject Strand: POSITIVE Subject Start: 93 Subject End: 291 .................................................................................................................................................................................................................................................................aaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt .................................................................................................................................................................................................................................................................aaactctaccttttactctaccttttttagataaatatatcatctggatattgaagttaagccctgctatcatttcgatggcagggctttttaatattt-gtacactaacttcttatctatattttggtggattttaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtatttt Subject: NC_008022_MGAS10270_Spy1908_MGAS10270_Spy1909|LSU ribosomal protein L33P:Cadmium resistance protein|POSITIVE:POSITIVE|[1861113,1861392]|280 HSP 1 e-value: 7.0E-85 bit: 315.0 Len: 187 Query Start:259 Query End:445 Subject Strand: POSITIVE Subject Start: 94 Subject End: 280 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatatatcatttggatattgaagttaagccctgctatcatttcgatggcagggctttttaatatttggtacactaacttcttatctatattttggtggagctgaagttgacatctacaaagtttaatgttaaaatacattcaaaaatatatttga............ Subject: NC_008023_MGAS2096_Spy1848_MGAS2096_Spy1849|LSU ribosomal protein L33P:Cadmium resistance protein|POSITIVE:POSITIVE|[1808727,1809006]|280 HSP 1 e-value: 5.0E-83 bit: 309.0 Len: 188 Query Start:258 Query End:445 Subject Strand: POSITIVE Subject Start: 93 Subject End: 280 .................................................................................................................................................................................................................................................................aaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ .................................................................................................................................................................................................................................................................aaactctaccttttactctaccttttttagataaatatatcatctggatattgaagttaagccctgctatcatttcgatggcagggctttttaatatttggtacactaacttattatctatattttggtggattttaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ Subject: NC_008021_MGAS9429_Spy1827_MGAS9429_Spy1828|LSU ribosomal protein L33P:cadmium resistance protein|POSITIVE:POSITIVE|[1784850,1785129]|280 HSP 1 e-value: 5.0E-83 bit: 309.0 Len: 188 Query Start:258 Query End:445 Subject Strand: POSITIVE Subject Start: 93 Subject End: 280 .................................................................................................................................................................................................................................................................aaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ .................................................................................................................................................................................................................................................................aaactctaccttttactctaccttttttagataaatatatcatctggatattgaagttaagccctgctatcatttcgatggcagggctttttaatatttggtacactaacttattatctatattttggtggattttaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ Subject: NC_007297_M5005_Spy_1816_M5005_Spy_1817|50S ribosomal protein L33:cadmium resistance protein|POSITIVE:POSITIVE|[1785518,1785809]|292 HSP 1 e-value: 1.0E-77 bit: 291.0 Len: 199 Query Start:259 Query End:457 Subject Strand: POSITIVE Subject Start: 94 Subject End: 292 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggtgtagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttgaatgaggtgtttt Subject: smit_SMT_1232_SMT_1233|hypothetical protein predicted by Glimmer/Critica:cadmium resistance protein|POSITIVE:POSITIVE|[1193270,1194445]|1176 HSP 1 e-value: 1.0E-74 bit: 281.0 Len: 185 Query Start:273 Query End:457 Subject Strand: POSITIVE Subject Start: 991 Subject End: 1176 ................................................................................................................................................................................................................................................................................ctctaccttttttagataaatatatcatctggatattgaagtt-aagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ................................................................................................................................................................................................................................................................................ctctaccttttttagataaatatatcatctggatattgaagtttaagccctgctgtcatttcgatggcagggctttttaatatttggtacactaacttcttatctatattttggtagattttaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtatttt Subject: NC_008024_MGAS10750_Spy1933_MGAS10750_Spy1934|LSU ribosomal protein L33P:Cadmium resistance protein|POSITIVE:POSITIVE|[1889180,1889460]|281 HSP 1 e-value: 6.0E-73 bit: 276.0 Len: 187 Query Start:259 Query End:445 Subject Strand: POSITIVE Subject Start: 95 Subject End: 281 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggtggagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttga............ Subject: NC_006086_M6_Spy1835_M6_Spy1836|50S ribosomal protein L33:Cadmium resistance protein|POSITIVE:POSITIVE|[1846355,1846634]|280 HSP 1 e-value: 6.0E-73 bit: 276.0 Len: 187 Query Start:259 Query End:445 Subject Strand: POSITIVE Subject Start: 94 Subject End: 280 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatctatcatttggatattgaagttaagccctgttatcatttcgatggcagggctttttaatatttggtacactaactccttatctatattttggtggagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttga............ Subject: NC_004606_SPs1815_SPs1816|50S ribosomal protein L33:putative cadmium resistance protein|POSITIVE:POSITIVE|[1842458,1842737]|280 HSP 1 e-value: 1.0E-71 bit: 272.0 Len: 185 Query Start:273 Query End:457 Subject Strand: POSITIVE Subject Start: 96 Subject End: 280 ................................................................................................................................................................................................................................................................................ctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ................................................................................................................................................................................................................................................................................ctctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggtgtagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttgaatgaggtgtttt Subject: NC_004070_SpyM3_1817_SpyM3_1818|50S ribosomal protein L33:putative cadmium resistance protein|POSITIVE:POSITIVE|[1848673,1848952]|280 HSP 1 e-value: 1.0E-71 bit: 272.0 Len: 185 Query Start:273 Query End:457 Subject Strand: POSITIVE Subject Start: 96 Subject End: 280 ................................................................................................................................................................................................................................................................................ctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ................................................................................................................................................................................................................................................................................ctctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggtgtagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttgaatgaggtgtttt Subject: NC_003485_spyM18_2197_spyM18_2198|50S ribosomal protein L33:putative cadmium resistance protein|POSITIVE:POSITIVE|[1843429,1843709]|281 HSP 1 e-value: 4.0E-71 bit: 270.0 Len: 188 Query Start:258 Query End:445 Subject Strand: POSITIVE Subject Start: 94 Subject End: 281 .................................................................................................................................................................................................................................................................aaactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ .................................................................................................................................................................................................................................................................aaactctaccttttactctaccttttttagatgaatctatcatttggatattgaagttaggccctgttatcatttcgatggcagggctttttaatatttggtacactaacttcttatctatattttggtggagctgaagttgacatctataaagtttaatgttaatatacattcaaaaatacatttga............ Subject: NC_007296_M28_Spy1825_M28_Spy1826|50S ribosomal protein L33:cadmium resistance protein|POSITIVE:POSITIVE|[1834062,1834341]|280 HSP 1 e-value: 2.0E-70 bit: 268.0 Len: 187 Query Start:259 Query End:445 Subject Strand: POSITIVE Subject Start: 94 Subject End: 280 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggtgtagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttga............ Subject: NC_004116_SAG1265_SAG1266|cadmium resistance transporter, putative:hypothetical protein|NEGATIVE:POSITIVE|[1275760,1276298]|539 HSP 1 e-value: 2.0E-70 bit: 268.0 Len: 179 Query Start:274 Query End:452 Subject Strand: NEGATIVE Subject Start: 274 Subject End: 452 .................................................................................................................................................................................................................................................................................tctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggt..... .................................................................................................................................................................................................................................................................................tctaccttttttagatgaatatgtcctttggatattgaagttaagccctgttatcatttggatggcagggcttttttatatttggtacactaacttcttatctatattttggcggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggt..... Subject: NC_002737_SPy2160_SPy2162|50S ribosomal protein L33:putative cadmium resistance protein|POSITIVE:POSITIVE|[1799434,1799725]|292 HSP 1 e-value: 2.0E-70 bit: 268.0 Len: 199 Query Start:259 Query End:457 Subject Strand: POSITIVE Subject Start: 94 Subject End: 292 ..................................................................................................................................................................................................................................................................aactctacctttttctctaccttttttagataaatatatcatctggatattgaagttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttgaatgaggtgtttt ..................................................................................................................................................................................................................................................................aactctaccttttactctaccttttttagatgaatatatcatttggatattgaagttaagccctgttatcatttggatgggcaggcttttttatatttggtacactaacttcttatctatattttggtgtagctgaaattgacatctacaaagtttaatgttaaaattcattcaaaaatatatttgaatgaggtgtttt Subject: NC_003116_NMA0495_NMA0496|50S ribosomal protein L31:cadmium resistance protein|POSITIVE:POSITIVE|[477858,478159]|302 HSP 1 e-value: 9.0E-23 bit: 109.0 Len: 71 Query Start:375 Query End:445 Subject Strand: POSITIVE Subject Start: 232 Subject End: 302 ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgatggggttgaagttgacatctacaaagtttaatgtttgaatacattaaaaaatatatttga............ HSP 2 e-value: 1.0E-15 bit: 85.7 Len: 67 Query Start:314 Query End:380 Subject Strand: POSITIVE Subject Start: 18 Subject End: 84 .........................................................................................................................................................................................................................................................................................................................ttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatat............................................................................. .........................................................................................................................................................................................................................................................................................................................ttaagccctgccatcatttcgatggcagggctttttaatatttggcatactgacttcttatctatat............................................................................. Subject: NC_008767_NMC1927_NMC1928|CaDmium resistance protein:50S ribosomal protein L31|NEGATIVE:NEGATIVE|[1955793,1956094]|302 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 71 Query Start:375 Query End:445 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 71 ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgacggggttgaagttgacatctacaaagtttaatgtttgaatacattaaaaaatatatttga............ HSP 2 e-value: 5.0E-18 bit: 93.7 Len: 67 Query Start:314 Query End:380 Subject Strand: NEGATIVE Subject Start: 219 Subject End: 285 .........................................................................................................................................................................................................................................................................................................................ttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatat............................................................................. .........................................................................................................................................................................................................................................................................................................................ttaagccctgccatcatttcgatggcagggctttttaatatttggcacactgacttcttatctatat............................................................................. Subject: NC_002946_NGO2126_NGO2127|50S ribosomal protein L31:CadD|POSITIVE:POSITIVE|[2109174,2109424]|251 HSP 1 e-value: 2.0E-20 bit: 101.0 Len: 71 Query Start:375 Query End:445 Subject Strand: POSITIVE Subject Start: 181 Subject End: 251 ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgacggggttgaagttgacatctacaaagtttaatgtttgaatacattaaaaaatatatttga............ HSP 2 e-value: 5.0E-18 bit: 93.7 Len: 67 Query Start:314 Query End:380 Subject Strand: POSITIVE Subject Start: 18 Subject End: 84 .........................................................................................................................................................................................................................................................................................................................ttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatat............................................................................. .........................................................................................................................................................................................................................................................................................................................ttaagccctgccatcatttcgatggcagggctttttaatatttggcacactgacttcttatctatat............................................................................. Subject: NC_003112_NMB1955_NMB1956|cadmium resistance protein:50S ribosomal protein L31|NEGATIVE:NEGATIVE|[2050975,2051276]|302 HSP 1 e-value: 5.0E-18 bit: 93.7 Len: 71 Query Start:375 Query End:445 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 71 ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgatggagctgaagttgacatctacaaagtttaatgttagaatacattcaaaaatatatttga............ ......................................................................................................................................................................................................................................................................................................................................................................................ctatattttgacggggttgaagttgacatctacaaggtttaatgtttgaatacatttaaaaatatatttga............ HSP 2 e-value: 5.0E-18 bit: 93.7 Len: 67 Query Start:314 Query End:380 Subject Strand: NEGATIVE Subject Start: 219 Subject End: 285 .........................................................................................................................................................................................................................................................................................................................ttaagccctgctataatttcgatggcagggctttttaatatttggtacactaccttcttatctatat............................................................................. .........................................................................................................................................................................................................................................................................................................................ttaagccctgccatcatttcgatggcagggctttttaatatttggcacactgacttcttatctatat............................................................................. Subject: NC_008532_STER_1954_STER_1955|Ribosomal protein L33:hypothetical protein|POSITIVE:NEGATIVE|[1810999,1811153]|155 HSP 1 e-value: 3.0E-13 bit: 77.8 Len: 51 Query Start:234 Query End:284 Subject Strand: POSITIVE Subject Start: 69 Subject End: 119 .........................................................................................................................................................................................................................................atatattgcactgtatttcaattcaaactctacctttttctctaccttttt............................................................................................................................................................................. .........................................................................................................................................................................................................................................atatattgaactgtatttcaattcaatctctacctttttctccaccttttt............................................................................................................................................................................. HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 44 Query Start:168 Query End:211 Subject Strand: POSITIVE Subject Start: 3 Subject End: 46 .......................................................................................................................................................................attcaatacgaatcaatacagaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... .......................................................................................................................................................................attcaatacgaatcaatagaaaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... Subject: NC_006449_str1975_str1976|50S ribosomal protein L33:hypothetical protein|POSITIVE:NEGATIVE|[1750945,1751164]|220 HSP 1 e-value: 3.0E-13 bit: 77.8 Len: 51 Query Start:234 Query End:284 Subject Strand: POSITIVE Subject Start: 69 Subject End: 119 .........................................................................................................................................................................................................................................atatattgcactgtatttcaattcaaactctacctttttctctaccttttt............................................................................................................................................................................. .........................................................................................................................................................................................................................................atatattgaactgtatttcaattcaatctctacctttttctccaccttttt............................................................................................................................................................................. HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 44 Query Start:168 Query End:211 Subject Strand: POSITIVE Subject Start: 3 Subject End: 46 .......................................................................................................................................................................attcaatacgaatcaatacagaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... .......................................................................................................................................................................attcaatacgaatcaatagaaaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... Subject: NC_006448_stu1975_stu1976|50S ribosomal protein L33:hypothetical protein|POSITIVE:NEGATIVE|[1749075,1749294]|220 HSP 1 e-value: 3.0E-13 bit: 77.8 Len: 51 Query Start:234 Query End:284 Subject Strand: POSITIVE Subject Start: 69 Subject End: 119 .........................................................................................................................................................................................................................................atatattgcactgtatttcaattcaaactctacctttttctctaccttttt............................................................................................................................................................................. .........................................................................................................................................................................................................................................atatattgaactgtatttcaattcaatctctacctttttctccaccttttt............................................................................................................................................................................. HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 44 Query Start:168 Query End:211 Subject Strand: POSITIVE Subject Start: 3 Subject End: 46 .......................................................................................................................................................................attcaatacgaatcaatacagaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... .......................................................................................................................................................................attcaatacgaatcaatagaaaagaaaaacgctgatttcaagcg...................................................................................................................................................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCAAUACGAAUCAAUACAGAAGAAAAACGCUGAUUUCAAGCGUUUUUUCUUUUUGCCAAAUGCGAUAUAUUGCACUGUAUUUCAAUUCAAACUCUACCUUUUUCUCUACCUUUUUUAGAUAAAUAUAUCAUCUGGAUAUUGAAGUUAAGCCCUGCUAUAAUUUCGAUGGCAGGGCUUUUUAAUAUUUGGUACACUACCUUCUUAUCUAUAUUUUGAUGGAGCUGAAGUUGACAUCUACAAAGUUUAAUGUUAGAAUACAUUCAAAAAUAUAUUUGAAUGAGGUGUUUU .........((((.(((((((..((((((((((.......))))))))))............(((((....))))))))))))...)))).........((((..(((((.(.....(((((((.........(..(((((((((...((((((((((((.......)))))))))))))))))))))..)............)))))))......).)))))..))))..((((((.(((........))).......((((((((......)))))))).)))))).. (-72.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAACACCUCAUUCAAAUAUAUUUUUGAAUGUAUUCUAACAUUAAACUUUGUAGAUGUCAACUUCAGCUCCAUCAAAAUAUAGAUAAGAAGGUAGUGUACCAAAUAUUAAAAAGCCCUGCCAUCGAAAUUAUAGCAGGGCUUAACUUCAAUAUCCAGAUGAUAUAUUUAUCUAAAAAAGGUAGAGAAAAAGGUAGAGUUUGAAUUGAAAUACAGUGCAAUAUAUCGCAUUUGGCAAAAAGAAAAAACGCUUGAAAUCAGCGUUUUUCUUCUGUAUUGAUUCGUAUUGAAU .........((((((((......)))))))).((((..((........(((((....((((.((((((((.(((........((((.((((.((((((.....))))))..(((((((((.............)))))))))..))))..))))...........(((((((.....)))))))......))).))).)))))))))..)))))..(((((((..((.....))...((((((((.((((.(....))))))))))))).)))))))....))...)))) (-64.22) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 402 200 152 166 120 NC_009009:SSA_1821|5end_Acetyltransferase_(N-acetylase_of_ribosomal_proteins),_putative_1810610..1810840_NEGATIVE 297 211 175 115 79 NC_009009:SSA_0556|5end_hypothetical_protein_551465..551695_POSITIVE 294 175 131 202 159 NC_009009:SSA_0050|5end_Dihydroxyacetone_kinase,_phosphatase_domain_(Dak2),_putative_54612..54842_POSITIVE 291 177 131 225 171 NC_009009:SSA_1823|5end_hypothetical_protein_1812513..1812743_NEGATIVE 281 205 167 160 129 NC_009009:SSA_0618|5end_hypothetical_protein_611973..612203_NEGATIVE 278 210 162 109 54 NC_009009:SSA_0322|5end_Transcriptional_activator,_TipA_family,_putative_314717..314947_POSITIVE 277 183 150 154 124 NC_009009:SSA_1373|5end_ATPase_components_of_ABC_transporters_with_duplicated_ATPase_domains,_multidrug_transport_system,_putative_1389707..1389937_POSITIVE 276 59 29 179 146 NC_009009:SSA_1724|5end_CBS_domain_protein,_putative_1710553..1710783_NEGATIVE 274 180 131 85 40 NC_009009:SSA_0567|5end_Isochorismitase_family_protein,_putative_560805..561035_POSITIVE 267 178 135 183 129 NC_009009:SSA_1448|5end_ATP_phosphoribosyltransferase_regulatory_subunit,_putative_1450817..1451047_NEGATIVE 267 112 75 84 46 NC_009009:SSA_0687|5end_hypothetical_protein_670467..670697_POSITIVE 265 178 131 134 89 NC_009009:SSA_1743|5end_ABC-type_Fe3+-siderophore_transport_system,_permease_component,_putative_1730170..1730400_POSITIVE 260 192 153 164 127 NC_009009:SSA_0509|5end_Propanediol_utilization_protein,_putative_508024..508254_POSITIVE 257 80 31 129 77 NC_009009:SSA_1845|5end_Serine/threonine_protein_kinase,_putative_1840181..1840411_NEGATIVE 256 180 135 199 148 NC_009009:SSA_0452|5end_Nitrogen_utilization_substance_protein_B,_putative_451534..451764_POSITIVE 254 182 144 60 10 NC_009009:SSA_1086|5end_TRNA_nucleotidyltransferase,_putative_1103328..1103558_POSITIVE 252 181 145 47 4 NC_009009:SSA_1855|5end_hypothetical_protein_1852051..1852281_NEGATIVE 249 177 132 219 177 NC_009009:SSA_1611|5end_Era-like_GTP-binding_protein,_putative_1616032..1616262_NEGATIVE 249 206 170 61 30 NC_009009:SSA_1582|5end_Esterase,_alpha/beta_hydrolase_superfamily,_putative_1584671..1584901_POSITIVE 246 237 191 64 19 NC_009009:SSA_2157|5end_ATP-dependent_serine_protease,_putative_2158241..2158471_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 315 58 25 91 53 NC_009009:SSA_2323|3end_Transporter,_putative_2328850..2329000_POSITIVE 314 50 3 77 27 NC_009009:SSA_0148|3end_Sugar_ABC_transporter,_ATP-binding_protein,_putative_143467..143617_POSITIVE 300 80 32 126 73 NC_009009:SSA_0963|3end_Peptidoglycan_N-acetylglucosamine_deacetylase_A,_putative_972890..973040_POSITIVE 277 55 13 89 52 NC_009009:SSA_0328|3end_Xaa-Pro_dipeptidyl-peptidase,_putative_320487..320637_NEGATIVE 276 53 20 92 48 NC_009009:SSA_0889|3end_Isoleucyl-tRNA_synthetase,_putative_882763..882913_NEGATIVE 276 56 20 91 47 NC_009009:SSA_0888|3end_Magnesium/cobalt_transporter,_putative_882749..882899_POSITIVE 274 193 151 69 16 NC_009009:SSA_0922|3end_hypothetical_protein_932225..932375_NEGATIVE 274 180 131 85 40 NC_009009:SSA_0566|3end_GTP-sensing_transcriptional_pleiotropic_repressor,_putative_560885..561035_POSITIVE 271 60 11 113 73 NC_009009:SSA_2068|3end_Superfamily_I_DNA_and_RNA_helicases,_putative_2070931..2071081_NEGATIVE 268 182 149 88 63 NC_009009:SSA_2235|3end_Conserved_uncharacterized_protein_2234971..2235121_NEGATIVE 267 178 135 127 73 NC_009009:SSA_1449|3end_Histidinol-phosphate/aromatic_aminotransferase_and_cobyric_acid_decarboxylase,_putative_1450873..1451023_NEGATIVE 260 192 153 124 87 NC_009009:SSA_0508|3end_Conserved_uncharacterized_protein,_possible_phosphoserine_phosphatase_508064..508214_POSITIVE 257 80 31 132 80 NC_009009:SSA_1846|3end_Phosphoprotein_phosphatase,_putative_1840178..1840328_NEGATIVE 257 116 71 134 93 NC_009009:SSA_1495|3end_S-adenosylmethionine_synthetase,_putative_1501019..1501169_NEGATIVE 254 182 144 63 13 NC_009009:SSA_1085|3end_Dihydrodipicolinate_reductase,_putative_1103411..1103561_POSITIVE 251 193 151 100 48 NC_009009:SSA_0470|3end_Precorrin-4_methylase,_putative_473732..473882_POSITIVE 249 206 170 80 49 NC_009009:SSA_1581|3end_Metal-binding_ABC_transporter_(probably_hemin),_putative_1584770..1584920_POSITIVE 247 183 149 62 33 NC_009009:SSA_0429|3end_Histidine_ammonia-lyase,_putative_429701..429851_NEGATIVE 245 192 151 132 78 NC_009009:SSA_1309|3end_RNA-binding_protein_(KH_domain),_putative_1327079..1327229_NEGATIVE 244 110 63 93 49 NC_009009:SSA_1467|3end_Chorismate_synthase,_putative_1473127..1473277_NEGATIVE