Origin IGS: tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaccaatagttgattttccttataaaaagtctttgttagctatagtcacggataaaaattatctagaatttaaaggagatcattttaaggtctcctttcttttttcttagctaaaaagttttttaatttctttgttgtaaacgcttgcaaaaatctggatagagtactataatagaattaaggaaatagaaagaaaggaaactaaattcattcgctttaaagtttactttgttggtatagttgtttgtaatacataagtagttgctttacttgacatattggttgaattgtcagacaggcctgcttagcttgcctgattgtaacgatagtatagaaagtagaagcttttagatggttttcaaacttcgagaattcaatcaatagcgctttattatattatagagatttccaatgctaagataaaaagaaaatcattagcggttgaaggaattaaaagtttataaaaaataataaaaacaataaatcaaaaaattgttattcagttacaccttggatgaactgattgctctcataccagcgaagtaaaccagacctctagccgagtaaagctagaggtcgttttttgtagacaagggtgtgaagaagacacacttgactttgaaaaaacatgaaaaaatgctataataggttggatatgcgaaccttaggtttgcaagactgtaattcgacttggctagccaaggaccgttagtaataaaataaagaatctttgataaaagtaaggttctgaaaattgataggagaaaaa Mask Tandem Repeat Region ================================================ tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Find is-nt database================================================ Query_seq: SSA_2146:SSA_2147|SSA_2146:SSA_2147:Metallo-beta-lactamase, putative:Conserved uncharacterized protein:<-<-:2148490..2149258 769 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_2146:SSA_2147|SSA_2146:SSA_2147:Metallo-beta-lactamase, putative:Conserved uncharacterized protein:<-<-:2148490..2149258 769 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_2146:SSA_2147|SSA_2146:SSA_2147:Metallo-beta-lactamase, putative:Conserved uncharacterized protein:<-<-:2148490..2149258 769 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 15 End: 197 .............. M F R T L L L S K I L Y F I T N G P W L A K S N Y S L A N L R F A Y P T Y Y S I F S C F F K V K C V F F T P L S T K N D ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 41 Strand: + Start: 115 End: 237 .................................................................................................................. M Q P I I A F F H V F S K S S V S S S H P C L Q K T T S S F T R L E V W F T S L V .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 54 Strand: + Start: 389 End: 550 .................................................................................................................................................................................................................................................................................................................................................................................................... M I E F S K F E N H L K A S T F Y T I V T I R Q A K Q A C L T I Q P I C Q V K Q L L M Y Y K Q L Y Q Q S K L ........................................................................................................................................................................................................................... Protein_Len: 42 Strand: + Start: 519 End: 644 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T N N Y T N K V N F K A N E F S F L S F Y F L N S I I V L Y P D F C K R L Q Q R N ............................................................................................................................. Protein_Len: 34 Strand: - Start: 663 End: 764 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... S F L F S V K F H D G K F E L Y N K D T V I A L L S K K Y P F D V M ..... Protein_Len: 34 Strand: - Start: 516 End: 617 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... T N C V V I G V F Y V K F R I F K T E K R E I E K I R N Y Y E I W M ........................................................................................................................................................ Protein_Len: 30 Strand: - Start: 309 End: 398 .................................................................................................................................................................................................................................................................................................................... L S K I G E V A L S K R K I K A N S I E I I Y Y L A I S Q M ................................................................................................................................................................................................................................................................................................................................................................................... tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 87 Start: 597 End: 626 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTGCAAAAATCTGGATAGAGTACTATAAT............................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 124 End: 153 ...........................................................................................................................CTTTGAAAAAACATGAAAAAATGCTATAAT........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 345 End: 374 ........................................................................................................................................................................................................................................................................................................................................................TTTTATCTTAGCATTGGAAATCTCTATAAT........................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 48 HP_score: -3.1 Tail_Score: -3.53444 Start: 153 End: 181 Full_Region: aggtcgttttttgta gacaagggtgt gaagaag acacacttgac tttgaaaaaacatga ........................................................................................................................................................gacaagggtgtgaagaagacacacttgac............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 60 HP_score: -4.9 Tail_Score: -3.94002 Start: 190 End: 222 Full_Region: CCTTGTCTACAAAAA ACGACCTCTAGCT TTACTC GGCTAGAGGTCTGG TTTACTTCGCTGGTA .............................................................................................................................................................................................ACGACCTCTAGCTTTACTCGGCTAGAGGTCTGG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 91 HP_score: -7.9 Tail_Score: -5.24129 Start: 191 End: 220 Full_Region: ccagcgaagtaaacc agacctctagc cgagtaaa gctagaggtcg ttttttgtagacaag ..............................................................................................................................................................................................agacctctagccgagtaaagctagaggtcg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 93 HP_score: -11.4 Tail_Score: -3.73995 Start: 192 End: 219 Full_Region: cagcgaagtaaacca gacctctagc cgagtaaa gctagaggtc gttttttgtagacaa ...............................................................................................................................................................................................gacctctagccgagtaaagctagaggtc...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 89 HP_score: -12.1 Tail_Score: -3.02881 Start: 192 End: 219 Full_Region: TTGTCTACAAAAAAC GACCTCTAGCT TTACTC GGCTAGAGGTC TGGTTTACTTCGCTG ...............................................................................................................................................................................................GACCTCTAGCTTTACTCGGCTAGAGGTC...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 74 HP_score: -8.9 Tail_Score: -3.06399 Start: 194 End: 217 Full_Region: GTCTACAAAAAACGA CCTCTAGCT TTACTC GGCTAGAGG TCTGGTTTACTTCGC .................................................................................................................................................................................................CCTCTAGCTTTACTCGGCTAGAGG........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 52 HP_score: -4.4 Tail_Score: -3.3197 Start: 453 End: 479 Full_Region: atattggttgaattg tcagacaggc ctgctta gcttgcctga ttgtaacgatagtat ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcagacaggcctgcttagcttgcctga.................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 84 HP_score: -5.3 Tail_Score: -5.4833 Start: 671 End: 699 Full_Region: aaattatctagaatt taaaggagatcat ttt aaggtctcctttc ttttttcttagctaa ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................taaaggagatcattttaaggtctcctttc...................................................................... TransTerm Strand: - Conf: 84 HP_score: -6.1 Tail_Score: -5.20735 Start: 675 End: 695 Full_Region: tatctagaatttaaa ggagatcat ttt aaggtctcc tttcttttttcttag ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ggagatcattttaaggtctcc.......................................................................... Find igs database================================================ Query_seq: SSA_2146:SSA_2147|SSA_2146:SSA_2147:Metallo-beta-lactamase, putative:Conserved uncharacterized protein:<-<-:2148490..2149258 769 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagcattttttcatgttttttcaaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataacaattttttgatttattgtttttattattttttataaacttttaattccttcaaccgctaatgattttctttttatcttagcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaacaaagaaattaaaaaactttttagctaagaaaaaagaaaggagaccttaaaatgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Intra-Species Hit: Count: 1 Min: 1 Max: 769 Len: 769 Subject: NC_009009_SSA_2146_SSA_2147|Metallo-beta-lactamase, putative:Conserved uncharacterized protein|NEGATIVE:NEGATIVE|[2148490,2149258]|769 HSP 1 e-value: 1.0E-158 bit: 559.0 Len: 282 Query Start:355 Query End:636 Subject Strand: POSITIVE Subject Start: 355 Subject End: 636 ..................................................................................................................................................................................................................................................................................................................................................................gcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaac..................................................................................................................................... ..................................................................................................................................................................................................................................................................................................................................................................gcattggaaatctctataatataataaagcgctattgattgaattctcgaagtttgaaaaccatctaaaagcttctactttctatactatcgttacaatcaggcaagctaagcaggcctgtctgacaattcaaccaatatgtcaagtaaagcaactacttatgtattacaaacaactataccaacaaagtaaactttaaagcgaatgaatttagtttcctttctttctatttccttaattctattatagtactctatccagatttttgcaagcgtttacaac..................................................................................................................................... HSP 2 e-value: 2.0E-68 bit: 262.0 Len: 132 Query Start:1 Query End:132 Subject Strand: POSITIVE Subject Start: 1 Subject End: 132 tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. tttttctcctatcaattttcagaaccttacttttatcaaagattctttattttattactaacggtccttggctagccaagtcgaattacagtcttgcaaacctaaggttcgcatatccaacctattatagca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 3 e-value: 4.0E-66 bit: 254.0 Len: 128 Query Start:149 Query End:276 Subject Strand: POSITIVE Subject Start: 149 Subject End: 276 ....................................................................................................................................................caaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataaca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ....................................................................................................................................................caaagtcaagtgtgtcttcttcacacccttgtctacaaaaaacgacctctagctttactcggctagaggtctggtttacttcgctggtatgagagcaatcagttcatccaaggtgtaactgaataaca............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. HSP 4 e-value: 1.0E-38 bit: 163.0 Len: 82 Query Start:688 Query End:769 Subject Strand: POSITIVE Subject Start: 688 Subject End: 769 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgatctcctttaaattctagataatttttatccgtgactatagctaacaaagactttttataaggaaaatcaactattggtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUCUCCUAUCAAUUUUCAGAACCUUACUUUUAUCAAAGAUUCUUUAUUUUAUUACUAACGGUCCUUGGCUAGCCAAGUCGAAUUACAGUCUUGCAAACCUAAGGUUCGCAUAUCCAACCUAUUAUAGCAUUUUUUCAUGUUUUUUCAAAGUCAAGUGUGUCUUCUUCACACCCUUGUCUACAAAAAACGACCUCUAGCUUUACUCGGCUAGAGGUCUGGUUUACUUCGCUGGUAUGAGAGCAAUCAGUUCAUCCAAGGUGUAACUGAAUAACAAUUUUUUGAUUUAUUGUUUUUAUUAUUUUUUAUAAACUUUUAAUUCCUUCAACCGCUAAUGAUUUUCUUUUUAUCUUAGCAUUGGAAAUCUCUAUAAUAUAAUAAAGCGCUAUUGAUUGAAUUCUCGAAGUUUGAAAACCAUCUAAAAGCUUCUACUUUCUAUACUAUCGUUACAAUCAGGCAAGCUAAGCAGGCCUGUCUGACAAUUCAACCAAUAUGUCAAGUAAAGCAACUACUUAUGUAUUACAAACAACUAUACCAACAAAGUAAACUUUAAAGCGAAUGAAUUUAGUUUCCUUUCUUUCUAUUUCCUUAAUUCUAUUAUAGUACUCUAUCCAGAUUUUUGCAAGCGUUUACAACAAAGAAAUUAAAAAACUUUUUAGCUAAGAAAAAAGAAAGGAGACCUUAAAAUGAUCUCCUUUAAAUUCUAGAUAAUUUUUAUCCGUGACUAUAGCUAACAAAGACUUUUUAUAAGGAAAAUCAACUAUUGGUU ..........((((((........(((((.............((((((.............(((..(((((....)))))))).......(.((((((((..((((((((((.............................(((((((...((.((((.((((.......)))).)))).))..)))))))(((((((((((......))))))))))).(((((((((.(.((((((((((....(((((((((.....))).)))))).............((.(((..((((.((........)).))))...))).)).(((((..(((((.(((.........)))))))).)))))..))))..(((((((.....(((.(((((.(((((((...(((((((.............))))))).......................(((((((.(((.....))).))))))).))))))).)))))))).(((((.......))))).))))))).........)))))).).))))))))).....))))).(((......)))..............)))))..(((...((((....))))..)))..))))))))).........)))))).........(((((((((((((((.....((((((((.(.......).))))))))...)))).((((.....))))........))))))).))))........)))))..........)))))). (-156.19) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCAAUAGUUGAUUUUCCUUAUAAAAAGUCUUUGUUAGCUAUAGUCACGGAUAAAAAUUAUCUAGAAUUUAAAGGAGAUCAUUUUAAGGUCUCCUUUCUUUUUUCUUAGCUAAAAAGUUUUUUAAUUUCUUUGUUGUAAACGCUUGCAAAAAUCUGGAUAGAGUACUAUAAUAGAAUUAAGGAAAUAGAAAGAAAGGAAACUAAAUUCAUUCGCUUUAAAGUUUACUUUGUUGGUAUAGUUGUUUGUAAUACAUAAGUAGUUGCUUUACUUGACAUAUUGGUUGAAUUGUCAGACAGGCCUGCUUAGCUUGCCUGAUUGUAACGAUAGUAUAGAAAGUAGAAGCUUUUAGAUGGUUUUCAAACUUCGAGAAUUCAAUCAAUAGCGCUUUAUUAUAUUAUAGAGAUUUCCAAUGCUAAGAUAAAAAGAAAAUCAUUAGCGGUUGAAGGAAUUAAAAGUUUAUAAAAAAUAAUAAAAACAAUAAAUCAAAAAAUUGUUAUUCAGUUACACCUUGGAUGAACUGAUUGCUCUCAUACCAGCGAAGUAAACCAGACCUCUAGCCGAGUAAAGCUAGAGGUCGUUUUUUGUAGACAAGGGUGUGAAGAAGACACACUUGACUUUGAAAAAACAUGAAAAAAUGCUAUAAUAGGUUGGAUAUGCGAACCUUAGGUUUGCAAGACUGUAAUUCGACUUGGCUAGCCAAGGACCGUUAGUAAUAAAAUAAAGAAUCUUUGAUAAAAGUAAGGUUCUGAAAAUUGAUAGGAGAAAAA ..((....((..((((((.((((.((..((((((((((((((.((((((((.......(((((((((....((((((((((.......))))))))))((((((........))))))................((((((....))))))...)))))))))....((((.((((..((((((.....(((.(((((....))...))).))).)))))).(((((((((((((((((.(((((((........(((((((.....)))))))...(((((((((((((.((...(((((..((...))..))))).(((.(((.((......((((((....))))))..)).)))..))).....)).))))))))))))).....(((((((.(((((((((((((((((((((((((.........))).))))).))))..)))))).....)))))))...)))))))))))))).............((...((((((.(((....).))))))))..))....)))))))))))))))))..((((((((((........))))))))))(((((((.((((...(((((((.......)))))))...)))))))))))...........)))).))))........((((((((...))))))))...)))).....))))))))))).)))))))..)).))))........((((((((..........))))))))))))))..)).))....... (-196.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 962 110 61 201 152 NC_009009:SSA_2146|5end_Metallo-beta-lactamase,_putative_2148399..2148629_NEGATIVE 365 239 200 164 118 NC_009009:SSA_0489|5end_Adenosylcobinamide_kinase_/_adenosylcobinamide_phosphate_guanyltransferase,_putative_489655..489885_POSITIVE 344 506 461 230 184 NC_009009:SSA_2024|5end_hypothetical_protein_2029272..2029502_NEGATIVE 341 239 194 153 99 NC_009009:SSA_0620|5end_hypothetical_protein_612325..612555_NEGATIVE 325 240 194 79 37 NC_009009:SSA_0997|5end_DNA-directed_DNA_polymerase_III_chain,_putative_996779..997009_POSITIVE 322 210 161 175 115 NC_009009:SSA_1481|5end_FmtA-like_protein,_putative_1486622..1486852_NEGATIVE 315 112 71 39 2 NC_009009:SSA_1938|5end_Enoyl-acyl_carrier_protein(ACP)_reductase,_putative_1925738..1925968_NEGATIVE 311 498 455 54 17 NC_009009:SSA_0195|5end_hypothetical_protein_195113..195343_POSITIVE 308 259 211 161 103 NC_009009:SSA_0512|5end_Nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase,_putative_510439..510669_POSITIVE 307 243 201 169 127 NC_009009:SSA_0825|5end_DNA-directed_RNA_polymerase,_sigma_subunit_(sigma70/sigma32),_putative_803864..804094_POSITIVE 304 250 201 228 185 NC_009009:SSA_2239|5end_Conserved_uncharacterized_cytosolic_protein_2237233..2237463_NEGATIVE 304 477 449 197 170 NC_009009:SSA_0481|5end_Cobyric_acid_synthase,_putative_481962..482192_POSITIVE 303 237 192 221 172 NC_009009:SSA_2170|5end_hypothetical_protein_2170240..2170470_NEGATIVE 302 250 202 176 132 NC_009009:SSA_2037|5end_tRNA_(Guanosine-2'-O-)-methyltransferase,_TrmH_family,_putative_2041524..2041754_NEGATIVE 301 254 219 41 1 NC_009009:SSA_1910|5end_hypothetical_protein_1902672..1902902_NEGATIVE 301 260 219 176 119 NC_009009:SSA_1180|5end_hypothetical_protein_1208559..1208789_NEGATIVE 298 233 194 225 191 NC_009009:SSA_1871|5end_Penicillin-binding_protein_2X,_putative_1868873..1869103_NEGATIVE 296 257 211 216 168 NC_009009:SSA_1468|5end_3-dehydroquinate_synthase,_putative_1475452..1475682_NEGATIVE 296 115 71 92 39 NC_009009:SSA_0508|5end_Conserved_uncharacterized_protein,_possible_phosphoserine_phosphatase_506668..506898_POSITIVE 295 264 223 69 18 NC_009009:SSA_1720|5end_Initiation-control_protein,_putative_1707113..1707343_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 485 441 57 12 NC_009009:SSA_0531|3end_Ethanolamine_utilization_protein_eutQ_(cobalamin-dependent_degradation_of_ethanolamine),_putative_527884..528034_POSITIVE 331 248 204 151 107 NC_009009:SSA_1418|3end_Conserved_uncharacterized_protein_1428139..1428289_NEGATIVE 331 233 195 151 115 NC_009009:SSA_1140|3end_Dihydrolipoamide_acetyl_transferase,_E2_component,_putative_1169207..1169357_POSITIVE 325 240 194 79 37 NC_009009:SSA_0996|3end_cGMP-specific_phosphodiesterase,_putative_996859..997009_POSITIVE 318 478 431 69 8 NC_009009:SSA_0148|3end_Sugar_ABC_transporter,_ATP-binding_protein,_putative_143467..143617_POSITIVE 309 236 191 136 94 NC_009009:SSA_0756|3end_Alanyl-tRNA_synthetase,_putative_739434..739584_POSITIVE 304 269 223 131 90 NC_009009:SSA_1459|3end_RNA_methyltransferase,_putative_1466111..1466261_NEGATIVE 303 499 451 73 12 NC_009009:SSA_1131|3end_High-affinity_Fe_2+/Pb2+_permease,_putative_1158768..1158918_POSITIVE 303 126 90 139 106 NC_009009:SSA_0245|3end_hypothetical_protein_239608..239758_POSITIVE 301 260 219 143 86 NC_009009:SSA_1181|3end_Hydrolase,_alpha/beta_superfamily,_putative_1208592..1208742_NEGATIVE 300 216 174 120 82 NC_009009:SSA_0419|3end_Alpha-galactosidase,_putative_418530..418680_POSITIVE 296 233 191 89 39 NC_009009:SSA_1710|3end_Transcriptional_regulator,_XRE_family,_putative_1699011..1699161_POSITIVE 295 109 61 83 30 NC_009009:SSA_0837|3end_Glucosyltransferase,_putative_825502..825652_POSITIVE 290 235 194 136 99 NC_009009:SSA_2013|3end_hypothetical_protein_2013470..2013620_NEGATIVE 289 236 191 107 59 NC_009009:SSA_1016|3end_Phosphoenolpyruvate_synthase,_putative_1022165..1022315_POSITIVE 286 105 62 54 8 NC_009009:SSA_2241|3end_Conserved_uncharacterized_protein_2237677..2237827_NEGATIVE 286 239 193 80 37 NC_009009:SSA_1753|3end_Transcriptional_regulator,_GntR_family_(repressor_of_trehalose_operon),_putative_1742740..1742890_POSITIVE 286 103 63 151 112 NC_009009:SSA_1416|3end_hypothetical_protein_1426520..1426670_NEGATIVE 286 219 176 136 90 NC_009009:SSA_0065|3end_Low_molecular_weight_phosphotyrosine_protein_phosphatase,_putative_67677..67827_POSITIVE 285 480 440 62 16 NC_009009:SSA_0585|3end_hypothetical_protein_577286..577436_POSITIVE