Origin IGS: agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaatagactgtaaagtcgtattctgccctcagagtatgatataatgattacgtttgaaaatcggagtgggaatgattccccgaaatacaaataggagaatggaact Mask Tandem Repeat Region ================================================ agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Find is-nt database================================================ Query_seq: SSA_2144:SSA_2145|SSA_2144:SSA_2145:Glutamyl-tRNA synthetase, putative:Tributyrin esterase, putative:<-<-:2145863..2145968 106 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_2144:SSA_2145|SSA_2144:SSA_2145:Glutamyl-tRNA synthetase, putative:Tributyrin esterase, putative:<-<-:2145863..2145968 106 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_2144:SSA_2145|SSA_2144:SSA_2145:Glutamyl-tRNA synthetase, putative:Tributyrin esterase, putative:<-<-:2145863..2145968 106 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 17 End: 106 ................ M Y F G E S F P L R F S N V I I I S Y S E G R I R L Y S L F agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_2144:SSA_2145|SSA_2144:SSA_2145:Glutamyl-tRNA synthetase, putative:Tributyrin esterase, putative:<-<-:2145863..2145968 106 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Intra-Species Hit: Count: 1 Min: 1 Max: 106 Len: 106 Subject: NC_009009_SSA_2144_SSA_2145|Glutamyl-tRNA synthetase, putative:Tributyrin esterase, putative|NEGATIVE:NEGATIVE|[2145863,2145968]|106 HSP 1 e-value: 6.0E-54 bit: 210.0 Len: 106 Query Start:1 Query End:106 Subject Strand: POSITIVE Subject Start: 1 Subject End: 106 agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt agttccattctcctatttgtatttcggggaatcattcccactccgattttcaaacgtaatcattatatcatactctgagggcagaatacgactttacagtctattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUCCAUUCUCCUAUUUGUAUUUCGGGGAAUCAUUCCCACUCCGAUUUUCAAACGUAAUCAUUAUAUCAUACUCUGAGGGCAGAAUACGACUUUACAGUCUAUUU ......((((((((....((((...(((((....))))).....((((.........))))........)))).....))).)))))..((((....))))..... (-17.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAGACUGUAAAGUCGUAUUCUGCCCUCAGAGUAUGAUAUAAUGAUUACGUUUGAAAAUCGGAGUGGGAAUGAUUCCCCGAAAUACAAAUAGGAGAAUGGAACU .......((((...(((((((((((....)))))))))))...........(((((....((((...((((....))))))))....)))))......)))).... (-21.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 875 50 1 141 92 NC_009009:SSA_2144|5end_Glutamyl-tRNA_synthetase,_putative_2145772..2146002_NEGATIVE 291 48 1 65 15 NC_009009:SSA_0509|5end_Propanediol_utilization_protein,_putative_508024..508254_POSITIVE 279 39 7 128 99 NC_009009:SSA_0688|5end_2,3-bisphosphoglycerate-dependent_phosphoglycerate_mutase,_putative_671360..671590_POSITIVE 279 85 41 142 97 NC_009009:SSA_0103|5end_Integral_membrane_protein,_putative_105490..105720_NEGATIVE 263 46 1 164 130 NC_009009:SSA_0538|5end_PduH_protein,_putative_533794..534024_POSITIVE 262 57 13 128 78 NC_009009:SSA_1942|5end_Enoyl-CoA_hydratase/carnithine_racemase,_putative_1928496..1928726_NEGATIVE 258 46 3 104 54 NC_009009:SSA_0774|5end_NADP-dependent_glyceraldehyde-3-phosphate_dehydrogenase,_putative_754571..754801_POSITIVE 255 47 7 73 37 NC_009009:SSA_1510|5end_Rhamnosyltransferase,_putative_1515336..1515566_NEGATIVE 253 88 42 115 69 NC_009009:SSA_1068|5end_Transcriptional_regulator,_LysR_family_(capsular_polysaccharide_biosynthesis_regulation),_putative_1087355..1087585_POSITIVE 247 78 32 147 88 NC_009009:SSA_0341|5end_hypothetical_protein_334731..334961_NEGATIVE 243 47 1 124 86 NC_009009:SSA_0596|5end_hypothetical_protein_586913..587143_POSITIVE 242 57 11 230 190 NC_009009:SSA_0490|5end_Cobalamin_5'-phosphate_synthase,_putative_490236..490466_POSITIVE 235 92 60 197 162 NC_009009:SSA_1011|5end_hypothetical_protein_1014134..1014364_POSITIVE 234 36 3 155 113 NC_009009:SSA_0134|5end_Membrane_carboxypeptidase_(penicillin-binding_protein),_putative_171990..172220_POSITIVE 233 48 1 113 66 NC_009009:SSA_1789|5end_Metal-dependent_phosphohydrolase,_HD_superfamily,_putative_1772856..1773086_NEGATIVE 232 52 16 175 134 NC_009009:SSA_2308|5end_hypothetical_protein_2310474..2310704_NEGATIVE 230 62 26 38 3 NC_009009:SSA_0911|5end_hypothetical_protein_922078..922308_POSITIVE 228 44 3 196 156 NC_009009:SSA_0247|5end_hypothetical_protein_240032..240262_POSITIVE 227 80 32 146 88 NC_009009:SSA_0686|5end_Fe2+/Zn2+_uptake_regulation_protein,_putative_669556..669786_POSITIVE 227 56 11 152 99 NC_009009:SSA_0217|5end_Two-component_system_transcriptional_regulator_(CheY_domain_and_HTH-like_DNA-binding_domain),_putative_213183..213413_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 46 8 107 71 NC_009009:SSA_0915|3end_hypothetical_protein_928014..928164_POSITIVE 244 57 11 120 78 NC_009009:SSA_1769|3end_hypothetical_protein_1756939..1757089_NEGATIVE 243 47 1 114 76 NC_009009:SSA_0595|3end_hypothetical_protein_586983..587133_POSITIVE 237 46 1 113 67 NC_009009:SSA_0040|3end_Phosphoribosylaminoimidazole_carboxylase,_ATPase_subunit,_putative_47479..47629_POSITIVE 236 58 11 113 56 NC_009009:SSA_0541|3end_Acetate_kinase,_putative_537082..537232_POSITIVE 233 48 1 68 21 NC_009009:SSA_1790|3end_SpoU_rRNA_Methylase_family_protein,_putative_1772901..1773051_NEGATIVE 230 62 26 41 6 NC_009009:SSA_0910|3end_ABC-type_multidrug_transporter,_ATPase_component,_putative_922161..922311_POSITIVE 218 43 1 42 8 NC_009009:SSA_1102|3end_hypothetical_protein_1123807..1123957_POSITIVE 218 57 25 39 1 NC_009009:SSA_0508|3end_Conserved_uncharacterized_protein,_possible_phosphoserine_phosphatase_508064..508214_POSITIVE 215 67 33 62 30 NC_009009:SSA_1850|3end_DNA-directed_RNA_polymerase,_subunit_K/omega,_putative_1845710..1845860_NEGATIVE 214 45 1 151 97 NC_009009:SSA_1960|3end_hypothetical_protein_1956571..1956721_NEGATIVE 209 70 22 93 43 NC_009009:SSA_2032|3end_Integrase/recombinase,_phage_integrase_family,_putative_2038307..2038457_POSITIVE 208 58 13 139 91 NC_009009:SSA_1258|3end_Purine_nucleoside_phosphorylase,_family_1,_putative_1283858..1284008_NEGATIVE 208 56 22 111 81 NC_009009:SSA_0929|3end_ABC-type_multidrug_transporter,_ATPase_and_permease_components,_putative_940263..940413_POSITIVE 206 24 4 37 18 NC_009009:SSA_2178|3end_hypothetical_protein_2176288..2176438_NEGATIVE 206 47 2 120 56 NC_009009:SSA_1013|3end_Phosphatidylserine_synthase,_putative_1017933..1018083_POSITIVE 205 39 1 119 69 NC_009009:SSA_1381|3end_hypothetical_protein_1399146..1399296_NEGATIVE 203 54 24 87 63 NC_009009:SSA_1612|3end_Diacylglycerol_kinase,_putative_1616099..1616249_NEGATIVE 203 46 1 111 53 NC_009009:SSA_1413|3end_GufA-like_protein,_putative_1424095..1424245_NEGATIVE 200 46 3 126 95 NC_009009:SSA_2131|3end_DNA-binding_protein,_putative_2134247..2134397_POSITIVE