Origin IGS: agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtgcgagacaaataaaccacccgctatgcgggtgcgcatcgaaggttataccaaaaaaactccaaacgcgatacaataaaggtgttcaagccaattgtgaagcgaaaggagaaaaatatggcgcaaaaggcacatagtttatcacacacaaagtggatgtgtaaatatcacattgtgtttacacctaagtataggtgaatcttatctataatcaatatcgaaacagtttgggtgaaatatttcaccgattatgtagttataaaggtgttgaaattatcgaaggtcacttgatgccggatcatgtacatatgttagtaagtattccaccaaggataagtgtttctagtttcatggggtatttaaaagggaaaagtgcactcatgatgtttgacaaacacgctaatctcaagtataagtttgggaaccgacatttttgggcggaaggctattacgtgagtacagtaggacttaatgaagccacaattaagaaatatatccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcccacatcaccagccagcgcctaaagacgctggctttcactttgttcaagcctcattgctcttgactcgtcacaaacctctcaaagaggtgtttgcattacttaccactatccctaaaggaatcctcatattcttttacactcaatttatccaacgctatatcatgcttttcctgttcttggatatatttcttaattgtggcttcattaagtcctactgtactcacgtaatagccttccgcccaaaaatgtcggttcccaaacttatacttgagattagcgtgtttgtcaaacatcatgagtgcacttttcccttttaaataccccatgaaactagaaacacttatccttggtggaatacttactaacatatgtacatgatccggcatcaagtgaccttcgataatttcaacacctttataactacataatcggtgaaatatttcacccaaactgtttcgatattgattatagataagattcacctatacttaggtgtaaacacaatgtgatatttacacatccactttgtgtgtgataaactatgtgccttttgcgccatatttttctcctttcgcttcacaattggcttgaacacctttattgtatcgcgtttggagtttttttggtataaccttcgatgcgcacccgcatagcgggtggtttatttgtctcgcaccttacggagcgagacagactgaaagtcacaaattaaaccacacccgcaagtccaaactcgcgggtgtttttatcaaaaaataaagtccaatcttgaacacagacctgtgttttatttggtctttttttatcccttgaggcggactatactagaagcatagaaatcaatcaatttttgaggaggaatggact Mask Tandem Repeat Region ================================================ agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtgcgagacaaataaaccacccgctatgcgggtgcgcatcgaaggttataccaaaaaaactccaaacgcgatacaataaaggtgttcaagccaattgtgaagcgaaaggagaaaaatatggcgcaaaaggcacatagtttatcacacacaaagtggatgtgtaaatatcacattgtgtttacacctaagtataggtgaatcttatctataatcaatatcgaaacagtttgggtgaaatatttcaccgattatgtagttataaaggtgttgaaattatcgaaggtcacttgatgccggatcatgtacatatgttagtaagtattccaccaaggataagtgtttctagtttcatggggtatttaaaagggaaaagtgcactcatgatgtttgacaaacacgctaatctcaagtataagtttgggaaccgacatttttgggcggaaggctattacgtgagtacagtaggacttaatgaagccacaattaagaaatatatccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Find is-nt database================================================ Query_seq: SSA_2076:SSA_2077|SSA_2076:SSA_2077:Conserved uncharacterized protein:hypothetical protein:<-<-:2080572..2081441 870 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtgcgagacaaataaaccacccgctatgcgggtgcgcatcgaaggttataccaaaaaaactccaaacgcgatacaataaaggtgttcaagccaattgtgaagcgaaaggagaaaaatatggcgcaaaaggcacatagtttatcacacacaaagtggatgtgtaaatatcacattgtgtttacacctaagtataggtgaatcttatctataatcaatatcgaaacagtttgggtgaaatatttcaccgattatgtagttataaaggtgttgaaattatcgaaggtcacttgatgccggatcatgtacatatgttagtaagtattccaccaaggataagtgtttctagtttcatggggtatttaaaagggaaaagtgcactcatgatgtttgacaaacacgctaatctcaagtataagtttgggaaccgacatttttgggcggaaggctattacgtgagtacagtaggacttaatgaagccacaattaagaaatatatccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 194 Max: 686 Len: 493 Subject: IS|IS200S HSP 1 e-value: 1.0E-139 bit: 496.0 Len: 493 Query Start:194 Query End:686 Subject Strand: POSITIVE Subject Start: 3204 Subject End: 3697 .................................................................................................................................................................................................gcgagacaaataaaccacccgctatgcgggtgcgcatcgaaggttataccnnnnnnnctccaaacgcgatacaataaaggtgttcaagccaattgtgaagcgaaaggagaaaaa-tatggcgcaaaaggcacatagtttatcacacacaaagtggatgtgtaaatatcacattgtgtttacacctaagtataggtgaatctt-atctataatcaatatcgaaacagtttgggtgaaatatttcaccgattatgtagttataaaggtgttgaaattatcgaaggtcacttgatgccggatcatgtacatatgttagtaagtattccaccaaggataagtgtttctagtttcatggggtatttaaaagggaaaagtgcactcatgatgtttgacaaacacgctaatctcaagtataagtttgggaaccgacatttttgggcggaaggctattacgtgagtacagtaggacttaatgaagccacaattaagaaatata........................................................................................................................................................................................ .................................................................................................................................................................................................gcgagacaaaacaaccacccgctatgcgggtgcgcgtcgaaggttataccaaaaaaactccaaacgcgatacaataaaggtgttcaatccacttgtaaagcgaaaggagaaaaaatatggcacaaaaggctcatagtttatctcacacaaagtggaggtgttc-tatcacattgtgttcacccctaagtatagacgaaaagtcatctataatcaatatagaagtagtttaggcgaaatatttcatcgcttgtgtagttataaaggtgttgaaatgatcgagggtcacttaatgccagaccatgtacacatgttagtcagtattccactaaggctaagtgtttcgagtttcatggggtatttaaaaggcaaaagtgcactcatgatgtttgacaaacacgccaatctcaagtacaagtttgggaatcggcatttctgggtggaaggttattatgtaagtacagtagggcttaataaagctacaattaagaaatata........................................................................................................................................................................................ Find is-aa database================================================ Query_seq: SSA_2076:SSA_2077|SSA_2076:SSA_2077:Conserved uncharacterized protein:hypothetical protein:<-<-:2080572..2081441 870 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_2076:SSA_2077|SSA_2076:SSA_2077:Conserved uncharacterized protein:hypothetical protein:<-<-:2080572..2081441 870 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 62 End: 163 ............................................................. M K K D Q I K H R S V F K I G L Y F L I K T P A S L D L R V W F N L ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 163 End: 267 .................................................................................................................................................................. M T F S L S R S V R C E T N K P P A M R V R I E G Y T K K T P N A I Q ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 41 Strand: + Start: 203 End: 325 .......................................................................................................................................................................................................... M N H P L C G C A S K V I P K K L Q T R Y N K G V Q A N C E A K G E K Y G A K G T ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 40 Strand: + Start: 267 End: 386 .......................................................................................................................................................................................................................................................................... M K V F K P I V K R K E K N M A Q K A H S L S H T K W M C K Y H I V F T P K Y R .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 307 End: 399 .................................................................................................................................................................................................................................................................................................................. M W R K R H I V Y H T Q S G C V N I T L C L H L S I G E S Y L ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 390 End: 479 ..................................................................................................................................................................................................................................................................................................................................................................................................... M L S I I N I E T V W V K Y F T D Y V V I K V L K L S K V T ....................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 386 End: 769 ................................................................................................................................................................................................................................................................................................................................................................................................. M N L I Y N Q Y R N S L G E I F H R L C S Y K G V E I I E G H L M P D H V H M L V S I P P R I S V S S F M G Y L K G K S ..................................................................................................... Protein_Len: 45 Strand: - Start: 643 End: 777 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. T L V T P S L S A V I L F Y I W S C S F C S I A N S L N L T F S Y S S E K L S L P L Y H M ............................................................................................. Protein_Len: 60 Strand: - Start: 554 End: 814 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... A I V H T C Y S K I F G C N L F I D L F L F L M I Y R Q I F Q T Y F F I L I G K P I T T L L A F V E K L P K H R T L L M ........................................................ Protein_Len: 36 Strand: - Start: 493 End: 600 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ T C I N T L I G G L I L T E L K M P Y K F P F L A S M I N S L C A L R M .............................................................................................................................................................................................................................................................................. Protein_Len: 44 Strand: - Start: 218 End: 349 ......................................................................................................................................................................................................................... A P A C R L N Y W F F E L R S V I F T N L G I T F R F S F F I A C F A C L K D C V F H M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 112 End: 267 ............................................................................................................... K K I F V G A L K S K R T H N L K H S E T Q R A G Y P A L C I F W G S H P H A D F T I G F F S W V R Y M ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 42 Strand: - Start: 35 End: 160 .................................. A E L I T R R L P Y F F V L Y F V P R H E L N S K I K Q Y F C G R T Q V Q P H P K M ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 87 Start: 578 End: 607 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTTGACAAACACGCTAATCTCAAGTATAAG....................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 20 End: 49 ...................ATTGATTGATTTCTATGCTTCTAGTATAGT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 264 End: 293 .......................................................................................................................................................................................................................................................................CTTCACAATTGGCTTGAACACCTTTATTGT................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 73 HP_score: -4.4 Tail_Score: -5.00923 Start: 83 End: 94 Full_Region: aagtccaatcttgaa caca gacc tgtg ttttatttggtcttt ..................................................................................cacagacctgtg........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 71 HP_score: -3.1 Tail_Score: -5.0917 Start: 94 End: 107 Full_Region: AAAACACAGGTCTGT GTTCA AGAT TGGAC TTTATTTTTTGATAA .............................................................................................GTTCAAGATTGGAC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 82 HP_score: -9.4 Tail_Score: -4.15669 Start: 123 End: 154 Full_Region: TTTATTTTTTGATAA AAACACCCGCGAGT TTGG ACTTGCGGGTGTGG TTTAATTTGTGACTT ..........................................................................................................................AAACACCCGCGAGTTTGGACTTGCGGGTGTGG............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 100 HP_score: -15.5 Tail_Score: -3.09117 Start: 126 End: 151 Full_Region: ATTTTTTGATAAAAA CACCCGCGAGT TTGG ACTTGCGGGTG TGGTTTAATTTGTGA .............................................................................................................................CACCCGCGAGTTTGGACTTGCGGGTG............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.3 Tail_Score: -4.83169 Start: 126 End: 151 Full_Region: tcacaaattaaacca cacccgcaagt ccaa actcgcgggtg tttttatcaaaaaat .............................................................................................................................cacccgcaagtccaaactcgcgggtg............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 87 HP_score: -12.3 Tail_Score: -3.09188 Start: 128 End: 149 Full_Region: TTTTTGATAAAAACA CCCGCGAGT TTGG ACTTGCGGG TGTGGTTTAATTTGT ...............................................................................................................................CCCGCGAGTTTGGACTTGCGGG................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 89 HP_score: -10.2 Tail_Score: -4.62441 Start: 208 End: 226 Full_Region: tataaccttcgatgc gcacccgc ata gcgggtgg tttatttgtctcgca ...............................................................................................................................................................................................................gcacccgcatagcgggtgg.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -13.7 Tail_Score: -3.36742 Start: 209 End: 225 Full_Region: ataaccttcgatgcg cacccgc ata gcgggtg gtttatttgtctcgc ................................................................................................................................................................................................................cacccgcatagcgggtg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 72 HP_score: -7.7 Tail_Score: -3.86897 Start: 781 End: 793 Full_Region: TAAGTAATGCAAACA CCTCT TTG AGAGG TTTGTGACGAGTCAA ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCTCTTTGAGAGG............................................................................. Find igs database================================================ Query_seq: SSA_2076:SSA_2077|SSA_2076:SSA_2077:Conserved uncharacterized protein:hypothetical protein:<-<-:2080572..2081441 870 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc Intra-Species Hit: Count: 1 Min: 1 Max: 870 Len: 870 Subject: NC_009009_SSA_2076_SSA_2077|Conserved uncharacterized protein:hypothetical protein|NEGATIVE:NEGATIVE|[2080572,2081441]|870 HSP 1 e-value: 2.0E-99 bit: 365.0 Len: 184 Query Start:687 Query End:870 Subject Strand: POSITIVE Subject Start: 687 Subject End: 870 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tccaagaacaggaaaagcatgatatagcgttggataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc HSP 2 e-value: 3.0E-92 bit: 341.0 Len: 193 Query Start:1 Query End:193 Subject Strand: POSITIVE Subject Start: 1 Subject End: 193 agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggatnnnnnnngaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggt..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... agtccattcctcctcaaaaattgattgatttctatgcttctagtatagtccgcctcaagggataaaaaaagaccaaataaaacacaggtctgtgttcaagattggactttattttttgataaaaacacccgcgagtttggacttgcgggtgtggtttaatttgtgactttcagtctgtctcgctccgtaaggt..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 2 Min: 719 Max: 870 Len: 152 Subject: NC_008533_SPD_0287_SPD_0289|hyaluronate lyase precursor:4-hydroxy-2-oxoglutarate aldolase/2-deydro-3-deoxyphosphogluconate aldolase|POSITIVE:NEGATIVE|[288390,289532]|1143 HSP 1 e-value: 1.0E-44 bit: 182.0 Len: 152 Query Start:719 Query End:870 Subject Strand: POSITIVE Subject Start: 947 Subject End: 1098 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gataaattgagtgtaaaagaatatgaggatccctttagggatagtggtaagtaataccaaagtctcttaaagaggcaagtgacgagtcaagagcaataaggcttgaacaacgtgaaagccagcgtctttaggcgctggctgatgatttgggc Subject: NC_003098_spr0286_spr0287|Hyaluronate lyase precursor (hyaluronidase/hyase):keto-hydroxyglutarate- aldolase/keto-deoxy- phosphogluconate aldolase|POSITIVE:NEGATIVE|[288340,289482]|1143 HSP 1 e-value: 1.0E-44 bit: 182.0 Len: 152 Query Start:719 Query End:870 Subject Strand: POSITIVE Subject Start: 947 Subject End: 1098 ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gataaattgagtgtaaaagaatatgaggattcctttagggatagtggtaagtaatgcaaacacctctttgagaggtttgtgacgagtcaagagcaatgaggcttgaacaaagtgaaagccagcgtctttaggcgctggctggtgatgtgggc ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gataaattgagtgtaaaagaatatgaggatccctttagggatagtggtaagtaataccaaagtctcttaaagaggcaagtgacgagtcaagagcaataaggcttgaacaacgtgaaagccagcgtctttaggcgctggctgatgatttgggc Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUAAAUUGAGUGUAAAAGAAUAUGAGGAUUCCUUUAGGGAUAGUGGUAAGUAAUGCAAACACCUCUUUGAGAGGUUUGUGACGAGUCAAGAGCAAUGAGGCUUGAACAAAGUGAAAGCCAGCGUCUUUAGGCGCUGGCUGGUGAUGUGGGC ......(..(((.(.......(((.(..(((((....)))))..).))).....(((....(((((.....)))))...(((....)))...)))...).)))..).((...((..((((((((((....))))))))))..))...))... (-41.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCCACAUCACCAGCCAGCGCCUAAAGACGCUGGCUUUCACUUUGUUCAAGCCUCAUUGCUCUUGACUCGUCACAAACCUCUCAAAGAGGUGUUUGCAUUACUUACCACUAUCCCUAAAGGAAUCCUCAUAUUCUUUUACACUCAAUUUAUC ((..(((((...((((((((.(....).))))))))....(((((....(((......)))..(((....))).........))))).)))))..))..................(((((((((......)))))))))............. (-31.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 340 149 102 210 158 NC_009009:SSA_0606|5end_ABC-type_antimicrobial_peptide_transport_system,_ATPase_component,_putative_594532..594762_NEGATIVE 337 140 92 217 173 NC_009009:SSA_1803|5end_GTP-binding_protein,_putative_1787909..1788139_NEGATIVE 329 143 101 77 27 NC_009009:SSA_1044|5end_Homoserine_kinase,_putative_1059853..1060083_POSITIVE 325 140 92 188 137 NC_009009:SSA_0875|5end_Rod_shape_determining_protein_(cell-cycle_protein),_putative_868928..869158_POSITIVE 322 150 101 226 177 NC_009009:SSA_2330|5end_Acetyltransferase,_putative_2333655..2333885_NEGATIVE 319 125 85 75 32 NC_009009:SSA_1269|5end_hypothetical_protein_1293352..1293582_NEGATIVE 317 150 101 84 34 NC_009009:SSA_1429|5end_hypothetical_protein_1437110..1437340_NEGATIVE 317 150 109 181 147 NC_009009:SSA_0244|5end_hypothetical_protein_238698..238928_POSITIVE 311 139 96 205 160 NC_009009:SSA_0481|5end_Cobyric_acid_synthase,_putative_481962..482192_POSITIVE 307 139 97 187 150 NC_009009:SSA_1483|5end_Sphingosine_kinase_(diacylglycerol_kinase),_putative_1490220..1490450_NEGATIVE 306 149 102 46 1 NC_009009:SSA_0780|5end_Acid_phosphatase_(PAP2_family),_putative_764748..764978_POSITIVE 306 109 64 59 14 NC_009009:SSA_0642|5end_Type_4_prepilin_peptidase,_putative_630053..630283_NEGATIVE 304 144 101 180 133 NC_009009:SSA_0138|5end_Metal-binding_(Zn)_permease,_putative_132533..132763_POSITIVE 303 150 118 174 134 NC_009009:SSA_1254|5end_hypothetical_protein_1281484..1281714_NEGATIVE 302 139 118 59 39 NC_009009:SSA_0964|5end_DEAD_RNA_helicase,_putative_974114..974344_NEGATIVE 297 130 82 177 138 NC_009009:SSA_1037|5end_Cytidine_deaminase,_putative_1052118..1052348_POSITIVE 295 139 92 178 124 NC_009009:SSA_0503|5end_Peptide_ABC_transporter,_ATP-binding_protein,_putative_502231..502461_POSITIVE 294 140 107 134 110 NC_009009:SSA_2137|5end_hypothetical_protein_2138412..2138642_NEGATIVE 293 138 92 106 68 NC_009009:SSA_2207|5end_hypothetical_protein_2207221..2207451_NEGATIVE 293 104 61 134 96 NC_009009:SSA_1291|5end_hypothetical_protein_1311102..1311332_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 370 126 89 50 12 NC_009009:SSA_2255|3end_Transcriptional_regulator,_XRE_family,_putative_2250730..2250880_POSITIVE 329 143 101 75 25 NC_009009:SSA_1043|3end_Homoserine_dehydrogenase,_putative_1059931..1060081_POSITIVE 317 150 101 87 37 NC_009009:SSA_1430|3end_Ribonuclease_Z,_putative_1437107..1437257_NEGATIVE 313 148 102 151 100 NC_009009:SSA_0607|3end_ABC_transporter,_permease_component,_putative_594590..594740_NEGATIVE 312 150 101 140 97 NC_009009:SSA_2338|3end_Conserved_uncharacterized_protein_2340392..2340542_POSITIVE 307 138 91 144 109 NC_009009:SSA_1053|3end_Pyruvate_phosphate_dikinase,_putative_1067447..1067597_NEGATIVE 302 139 118 138 118 NC_009009:SSA_0965|3end_Oxidoreductase,_putative_974035..974185_NEGATIVE 294 140 107 84 60 NC_009009:SSA_2138|3end_RNA-binding_protein,_Jag_family,_putative_2138462..2138612_NEGATIVE 293 104 61 137 99 NC_009009:SSA_1292|3end_hypothetical_protein_1311099..1311249_NEGATIVE 293 120 71 45 3 NC_009009:SSA_0643|3end_hypothetical_protein_630072..630222_NEGATIVE 292 150 117 127 98 NC_009009:SSA_2254|3end_Holliday_junction_DNA_helicase_ruvA,_putative_2249434..2249584_NEGATIVE 292 126 83 67 18 NC_009009:SSA_1323|3end_hypothetical_protein_1339788..1339938_NEGATIVE 292 150 102 81 23 NC_009009:SSA_0470|3end_Precorrin-4_methylase,_putative_473732..473882_POSITIVE 289 148 101 89 43 NC_009009:SSA_1375|3end_Multidrug_ABC_transporter,_ATPase_and_permease_components,_putative_1393085..1393235_NEGATIVE 286 141 101 79 32 NC_009009:SSA_0899|3end_Conserved_permease,_putative_891161..891311_POSITIVE 285 143 109 74 42 NC_009009:SSA_1552|3end_hypothetical_protein_1555130..1555280_POSITIVE 280 149 102 68 2 NC_009009:SSA_2034|3end_50S_ribosomal_protein_L13,_putative_2038798..2038948_NEGATIVE 280 146 117 78 42 NC_009009:SSA_0635|3end_Indole-3-glycerol_phosphate_synthase,_putative_625235..625385_POSITIVE 279 120 71 140 95 NC_009009:SSA_1665|3end_hypothetical_protein_1665222..1665372_NEGATIVE 278 139 91 74 21 NC_009009:SSA_1590|3end_Transcriptional_regulator,_TetR/AcrR_family,_putative_1593342..1593492_POSITIVE