Origin IGS: gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttaaaacaaacaaaagctttccgtttaggaggctttttgtttttacttcgtttccaaaatatgacatctgtcattttgtttttgtgacaaacgttaggtgaagcgcttacaaaaatttgttatatttagattattgagattgcttcttttttataaaggagttaggtgctaaacgaggtgttcattttgaacaagaaaaggtgaaaggagtgcgttatgtagcagtaatttggttgtgaggagtctgattttatttttagaatattgaggtgtgtc Mask Tandem Repeat Region ================================================ gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Find is-nt database================================================ Query_seq: SSA_2023:SSA_2024|SSA_2023:SSA_2024:Fructan beta-fructosidase precursor, putative:hypothetical protein:<-<-:2028917..2029194 278 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_2023:SSA_2024|SSA_2023:SSA_2024:Fructan beta-fructosidase precursor, putative:hypothetical protein:<-<-:2028917..2029194 278 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_2023:SSA_2024|SSA_2023:SSA_2024:Fructan beta-fructosidase precursor, putative:hypothetical protein:<-<-:2028917..2029194 278 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 14 End: 112 ............. M L K I K S D S S Q P N Y C Y I T H S F H L F L F K M N T S F S T ...................................................................................................................................................................... Protein_Len: 39 Strand: + Start: 161 End: 277 ................................................................................................................................................................ M F V S A S P N V C H K N K M T D V I F W K R S K N K K P P K R K A F V C F K . gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 81 HP_score: -4.4 Tail_Score: -5.35224 Start: 204 End: 215 Full_Region: ttcgtttccaaaata tgac atct gtca ttttgtttttgtgac ...........................................................................................................................................................................................................tgacatctgtca............................................................... Find igs database================================================ Query_seq: SSA_2023:SSA_2024|SSA_2023:SSA_2024:Fructan beta-fructosidase precursor, putative:hypothetical protein:<-<-:2028917..2029194 278 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Intra-Species Hit: Count: 1 Min: 1 Max: 278 Len: 278 Subject: NC_009009_SSA_2023_SSA_2024|Fructan beta-fructosidase precursor, putative:hypothetical protein|NEGATIVE:NEGATIVE|[2028917,2029194]|278 HSP 1 e-value: 1.0E-156 bit: 551.0 Len: 278 Query Start:1 Query End:278 Subject Strand: POSITIVE Subject Start: 1 Subject End: 278 gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat gacacacctcaatattctaaaaataaaatcagactcctcacaaccaaattactgctacataacgcactcctttcaccttttcttgttcaaaatgaacacctcgtttagcacctaactcctttataaaaaagaagcaatctcaataatctaaatataacaaatttttgtaagcgcttcacctaacgtttgtcacaaaaacaaaatgacagatgtcatattttggaaacgaagtaaaaacaaaaagcctcctaaacggaaagcttttgtttgttttaaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACACACCUCAAUAUUCUAAAAAUAAAAUCAGACUCCUCACAACCAAAUUACUGCUACAUAACGCACUCCUUUCACCUUUUCUUGUUCAAAAUGAACACCUCGUUUAGCACCUAACUCCUUUAUAAAAAAGAAGCAAUCUCAAUAAUCUAAAUAUAACAAAUUUUUGUAAGCGCUUCACCUAACGUUUGUCACAAAAACAAAAUGACAGAUGUCAUAUUUUGGAAACGAAGUAAAAACAAAAAGCCUCCUAAACGGAAAGCUUUUGUUUGUUUUAAAU .......................(((((.(((((..................(((........)))...(((((.........(((((.....)))))...(((((((......................(((((..(..(((.(((.............))).)))..)..))))).....((((((((((...........))))))))))..(((((((....)))))))...............)))))))))))).....))))))))))... (-43.12) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUAAAACAAACAAAAGCUUUCCGUUUAGGAGGCUUUUUGUUUUUACUUCGUUUCCAAAAUAUGACAUCUGUCAUUUUGUUUUUGUGACAAACGUUAGGUGAAGCGCUUACAAAAAUUUGUUAUAUUUAGAUUAUUGAGAUUGCUUCUUUUUUAUAAAGGAGUUAGGUGCUAAACGAGGUGUUCAUUUUGAACAAGAAAAGGUGAAAGGAGUGCGUUAUGUAGCAGUAAUUUGGUUGUGAGGAGUCUGAUUUUAUUUUUAGAAUAUUGAGGUGUGUC ....((((((((...((((((.((.....))))))))))))))))..((((((..(((((......(((((....((((((((..((((...((.((..((..(((((((((((.((((((.......)))))).))).....((((((((.....))))))))))))))))..)))).))..))))...))))))))..))))).....(.(((........))).)..)))))..))))))..(((((........)))))............... (-62.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 901 80 31 171 122 NC_009009:SSA_2023|5end_Fructan_beta-fructosidase_precursor,_putative_2028826..2029056_NEGATIVE 301 100 52 227 170 NC_009009:SSA_0087|5end_V-type_sodium_ATPase,_subunit_E,_putative_88329..88559_POSITIVE 279 209 167 160 114 NC_009009:SSA_0441|5end_hypothetical_protein_441481..441711_POSITIVE 260 75 32 105 56 NC_009009:SSA_1415|5end_Oxidoreductase,_putative_1426508..1426738_NEGATIVE 258 93 52 115 64 NC_009009:SSA_0900|5end_Tetratricopeptide_repeat_(TPR)_family_protein_891068..891298_POSITIVE 258 134 92 134 90 NC_009009:SSA_0374|5end_Peptide_methionine_sulfoxide_reductase_msrA/msrB,_putative_363163..363393_POSITIVE 257 110 64 203 159 NC_009009:SSA_0926|5end_Histone_acetyltransferase_HPA2,_putative_935465..935695_POSITIVE 247 110 63 98 38 NC_009009:SSA_2349|5end_DTDP-4-dehydrorhamnose_3,5-epimerase,_putative_2351708..2351938_NEGATIVE 247 264 235 102 73 NC_009009:SSA_2054|5end_hypothetical_protein_2057899..2058129_NEGATIVE 246 109 64 134 88 NC_009009:SSA_1059|5end_hypothetical_protein_1076153..1076383_POSITIVE 243 103 63 70 21 NC_009009:SSA_1572|5end_hypothetical_protein_1575908..1576138_NEGATIVE 242 270 221 190 130 NC_009009:SSA_0875|5end_Rod_shape_determining_protein_(cell-cycle_protein),_putative_868928..869158_POSITIVE 241 100 51 138 84 NC_009009:SSA_0390|5end_hypothetical_protein_385002..385232_POSITIVE 239 75 31 103 51 NC_009009:SSA_0270|5end_Phosphotransferase_system_(PTS)_cellobiose-specific,_IIC_component,_putative_262873..263103_POSITIVE 238 75 31 62 8 NC_009009:SSA_1602|5end_Exoribonuclease,_VacB/Rnb_family,_putative_1609370..1609600_NEGATIVE 238 109 67 141 98 NC_009009:SSA_1382|5end_hypothetical_protein_1400270..1400500_NEGATIVE 238 135 97 225 186 NC_009009:SSA_0334|5end_Diphosphomevalonate_decarboxylase,_putative_327855..328085_POSITIVE 237 85 50 46 14 NC_009009:SSA_0026|5end_Fatty_acid/phospholipid_synthesis_protein_plsX,_putative_30473..30703_POSITIVE 236 95 51 71 29 NC_009009:SSA_0769|5end_hypothetical_protein_749216..749446_NEGATIVE 236 95 51 192 150 NC_009009:SSA_0768|5end_Ribonucleoside-diphosphate_reductase_2,_beta_subunit,_putative_749095..749325_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 209 167 64 18 NC_009009:SSA_0440|3end_30S_ribosomal_protein_S18,_putative_441465..441615_POSITIVE 260 75 32 93 44 NC_009009:SSA_1416|3end_hypothetical_protein_1426520..1426670_NEGATIVE 258 93 52 128 77 NC_009009:SSA_0899|3end_Conserved_permease,_putative_891161..891311_POSITIVE 247 264 235 65 36 NC_009009:SSA_2055|3end_hypothetical_protein_2057936..2058086_NEGATIVE 247 70 28 84 42 NC_009009:SSA_1672|3end_hypothetical_protein_1670291..1670441_NEGATIVE 246 109 64 112 66 NC_009009:SSA_1058|3end_Thiamine_biosynthesis_protein,_putative_1076211..1076361_POSITIVE 241 100 51 138 84 NC_009009:SSA_0389|3end_hypothetical_protein_385082..385232_POSITIVE 240 109 64 151 108 NC_009009:SSA_0925|3end_ABC-type_antimicrobial_peptide_transport_system,_ATPase_component,_putative_935494..935644_POSITIVE 239 75 31 97 45 NC_009009:SSA_0269|3end_Phosphotransferase_system_(PTS)_cellobiose-specific_component_IIA,_putative_262947..263097_POSITIVE 237 85 50 40 8 NC_009009:SSA_0025|3end_DNA_repair_protein_recO,_putative_30547..30697_POSITIVE 235 119 75 114 65 NC_009009:SSA_1129|3end_Periplasmic_iron_transport_lipoprotein,_putative_1155877..1156027_POSITIVE 232 215 174 130 97 NC_009009:SSA_1660|3end_ABC-type_antimicrobial_peptide_transport_system,_ATPase_component,_putative_1656819..1656969_NEGATIVE 230 217 171 121 76 NC_009009:SSA_1190|3end_Conserved_uncharacterized_protein_1217799..1217949_NEGATIVE 230 78 33 92 46 NC_009009:SSA_0475|3end_CbiK_protein,_putative_478570..478720_POSITIVE 230 89 41 146 92 NC_009009:SSA_0250|3end_GTP_pyrophosphokinase,_putative_244466..244616_POSITIVE 229 88 52 124 95 NC_009009:SSA_1448|3end_ATP_phosphoribosyltransferase_regulatory_subunit,_putative_1449828..1449978_NEGATIVE 229 68 29 46 6 NC_009009:SSA_0689|3end_Penicillin-binding_protein_2B,_putative_674408..674558_POSITIVE 228 119 71 112 64 NC_009009:SSA_1353|3end_hypothetical_protein_1367974..1368124_NEGATIVE 228 89 49 97 60 NC_009009:SSA_1188|3end_Ribonuclease_HII,_putative_1216055..1216205_NEGATIVE 228 89 58 97 65 NC_009009:SSA_0650|3end_GTP-binding_protein_TypA/BipA,_putative_634604..634754_POSITIVE