Origin IGS: cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tggttcttttagaacagtgcaatttttggataatcgcgtggaaattttagattttcatgaggaaagtccatgctagcactggctgtgatgccggtagtgtttgtgctaggcgaagaaataagcctagggacggatttttccgttacggcgactgaaagggctaagtctttggatatgtctgattaggtctgaaagtgccacagtgacgtagttcttttggaaacagaagaagtggaacgcggtaaacccctcaagctagcaacccaaactttggtcggggcatggagtgcgtggaagcgaacatagcactctgactggaaacagtagacagatgattatcgaaggaaatggtacctagtcatttccggaacaaaacatggcttacagaaaattgcatataggctgaggctaggacgtcaagtcctgtcctctttaaattttataatagaatagcaagagagtggctgagcgctctattctctgccaagttcaactagctgctgcttcatttgtctg Mask Tandem Repeat Region ================================================ cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Find is-nt database================================================ Query_seq: SSA_1856:SSA_1857|SSA_1856:SSA_1857:hypothetical protein:Conserved DivIVA-like protein, putative:<-<-:1852299..1852814 516 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1856:SSA_1857|SSA_1856:SSA_1857:hypothetical protein:Conserved DivIVA-like protein, putative:<-<-:1852299..1852814 516 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1856:SSA_1857|SSA_1856:SSA_1857:hypothetical protein:Conserved DivIVA-like protein, putative:<-<-:1852299..1852814 516 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 168 End: 263 ....................................................................................................................................................................... M S F D N H L S T V S S Q S A M F A S T H S M P R P K F G L L A ............................................................................................................................................................................................................................................................. Protein_Len: 55 Strand: + Start: 341 End: 505 .................................................................................................................................................................................................................................................................................................................................................... M S K D L A L S V A V T E K S V P R L I S S P S T N T T G I T A S A S M D F P H E N L K F P R D Y P K I A L F ........... Protein_Len: 34 Strand: - Start: 365 End: 466 ............................................................................................................................................................................................................................................................................................................................................................................ D G Y R F F G D R P K N R R R A C V S G A D C G T S A H V K R M F M .................................................. Protein_Len: 38 Strand: - Start: 334 End: 447 ............................................................................................................................................................................................................................................................................................................................................. D S M D L S K A R E T A T V S F D T G L S I E E G L V F V V P M V A L A L M ..................................................................... Protein_Len: 30 Strand: - Start: 67 End: 156 .................................................................. E I I F N L S S L V Q R G L R L R Y A I K Q L G H K T G S M ........................................................................................................................................................................................................................................................................................................................................................................ cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 74 HP_score: -8.7 Tail_Score: -3.97308 Start: 86 End: 112 Full_Region: aattgcatataggct gaggctaggac gtcaa gtcctgtcctc tttaaattttataat .....................................................................................gaggctaggacgtcaagtcctgtcctc.................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 45 HP_score: -3.5 Tail_Score: -3.11192 Start: 151 End: 173 Full_Region: GCCATGTTTTGTTCC GGAAATG ACTAGGTAC CATTTCC TTCGATAATCATCTG ......................................................................................................................................................GGAAATGACTAGGTACCATTTCC....................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: SSA_1856:SSA_1857|SSA_1856:SSA_1857:hypothetical protein:Conserved DivIVA-like protein, putative:<-<-:1852299..1852814 516 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Intra-Species Hit: Count: 1 Min: 1 Max: 516 Len: 516 Subject: NC_009009_SSA_1856_SSA_1857|hypothetical protein:Conserved DivIVA-like protein, putative|NEGATIVE:NEGATIVE|[1852299,1852814]|516 HSP 1 e-value: 0.0 bit: 1023.0 Len: 516 Query Start:1 Query End:516 Subject Strand: POSITIVE Subject Start: 1 Subject End: 516 cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca cagacaaatgaagcagcagctagttgaacttggcagagaatagagcgctcagccactctcttgctattctattataaaatttaaagaggacaggacttgacgtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttccagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcactgttctaaaagaacca Inter-species Hit: Count: 21 Min: 102 Max: 501 Len: 400 Subject: NC_006449_str0233_str0234|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[237446,237971]|526 HSP 1 e-value: 1.0E-86 bit: 321.0 Len: 360 Query Start:102 Query End:461 Subject Strand: NEGATIVE Subject Start: 48 Subject End: 408 .....................................................................................................gtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttc-cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatg....................................................... .....................................................................................................gtcccagcctca-cctatatgcaattttctataagccatgttctgttccagaagtgactaggtaccacttccttcgataatcatctgtctactgcatagcagtcagaatggtccgttcgtttccggccatcccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccactttttaggtttccctaaaaactccgtcactgtggcactttcagagctactaaaacctatccgaagacttagccagttcgctcgccgtaacttgaaaatcaagtccctaggcttattgattcgcctagcacaaacactactggcatcacagccagtgctagcatggactttcctcatg....................................................... Subject: NC_006448_stu0233_stu0234|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[237407,237932]|526 HSP 1 e-value: 1.0E-86 bit: 321.0 Len: 360 Query Start:102 Query End:461 Subject Strand: NEGATIVE Subject Start: 48 Subject End: 408 .....................................................................................................gtcctagcctcagcctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactgtttc-cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatg....................................................... .....................................................................................................gtcccagcctca-cctatatgcaattttctataagccatgttctgttccagaagtgactaggtaccacttccttcgataatcatctgtctactgcatagcagtcagaatggtccgttcgtttccggccatcccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccactttttaggtttccctaaaaactccgtcactgtggcactttcagagctactaaaacctatccgaagacttagccagttcgctcgccgtaacttgaaaatcaagtccctaggcttattgattcgcctagcacaaacactactggcatcacagccagtgctagcatggactttcctcatg....................................................... Subject: smit_SMT_301_SMT_302|hypothetical protein:K07444 putative N6-adenine-specific DNA methylase|POSITIVE:POSITIVE|[244463,244947]|485 HSP 1 e-value: 4.0E-62 bit: 240.0 Len: 302 Query Start:200 Query End:501 Subject Strand: NEGATIVE Subject Start: 15 Subject End: 314 .......................................................................................................................................................................................................cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttc--cacgcgattatccaaaaattgcact............... .......................................................................................................................................................................................................cagtcagagtactacgttcgtttccgtgcactccatgccccgaccgaaatttgggttgctagcttgaggggtttaccgcgttccactctctccgtttccagaaagactccgtcactgtggcactttcaagcctactctgacctatccgaagacttagccatttcaactgccgtaacg---atctctcgtccctaggcttatggtttcgcctagcacaaacactacaggcatcacagcctgtgctagcatggactttcctcatgaaaa--gaaattctcctcacgcgattatccaaaaattgcact............... HSP 2 e-value: 2.0E-20 bit: 101.0 Len: 79 Query Start:115 Query End:193 Subject Strand: NEGATIVE Subject Start: 338 Subject End: 415 ..................................................................................................................cctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................cctatatgcaattttctataagccatgttttgttccagaagtgactagg-accacttcctttgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_008533_SPD_0339_SPD_0341|hypothetical protein:RNA methylase family protein UPF0020, putative|POSITIVE:POSITIVE|[344137,344621]|485 HSP 1 e-value: 4.0E-59 bit: 230.0 Len: 301 Query Start:200 Query End:500 Subject Strand: NEGATIVE Subject Start: 16 Subject End: 314 .......................................................................................................................................................................................................cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................cagtcagaatactacgttcgtttccgcgtactccatgccccgaccaaaatttgggttgctagcttgaggggtttaccgcgttccactctctctgtttccagaaagactccgtcactgtggcactttcaagcctactctggcctatccaaggacttagccatttcaactgccgtaacg---atttctcgtccctaggcttatggtttcgcctagcacaaacactacaggcatcacagcctgtgctagcatggactttcctcatgagaagcaattctcctcacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 79 Query Start:115 Query End:193 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 414 ..................................................................................................................cctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................cctatatgcaattttctataagccatgttttgttccagaagtgactagg-accacttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_003098_spr0332_spr0333|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[336608,337077]|470 HSP 1 e-value: 4.0E-59 bit: 230.0 Len: 301 Query Start:200 Query End:500 Subject Strand: NEGATIVE Subject Start: 16 Subject End: 314 .......................................................................................................................................................................................................cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................cagtcagaatactacgttcgtttccgcgtactccatgccccgaccaaaatttgggttgctagcttgaggggtttaccgcgttccactctctctgtttccagaaagactccgtcactgtggcactttcaagcctactctggcctatccaaggacttagccatttcaactgccgtaacg---atttctcgtccctaggcttatggtttcgcctagcacaaacactacaggcatcacagcctgtgctagcatggactttcctcatgagaagcaattctcctcacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 79 Query Start:115 Query End:193 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 414 ..................................................................................................................cctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................cctatatgcaattttctataagccatgttttgttccagaagtgactagg-accacttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_003028_SP_0372_SP_0373|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[350598,351082]|485 HSP 1 e-value: 4.0E-59 bit: 230.0 Len: 301 Query Start:200 Query End:500 Subject Strand: NEGATIVE Subject Start: 16 Subject End: 314 .......................................................................................................................................................................................................cagtcagagtgctatgttcgcttccacgcactccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatc-cgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................cagtcagaatactacgttcgtttccgcgtactccatgccccgaccaaaatttgggttgctagcttgaggggtttaccgcgttccactctctctgtttccagaaagactccgtcactgtggcactttcaagcctactctggcctatccaaggacttagccatttcaactgccgtaacg---atttctcgtccctaggcttatggtttcgcctagcacaaacactacaggcatcacagcctgtgctagcatggactttcctcatgagaaacaattctcctcacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 79 Query Start:115 Query End:193 Subject Strand: NEGATIVE Subject Start: 337 Subject End: 414 ..................................................................................................................cctatatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ..................................................................................................................cctatatgcaattttctataagccatgttttgttccagaagtgactagg-accacttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_004350_SMU.471_SMU.472|hypothetical protein:conserved hypothetical protein; possible N6-adenine-specific DNA methylase|POSITIVE:POSITIVE|[439785,440233]|449 HSP 1 e-value: 2.0E-54 bit: 214.0 Len: 269 Query Start:231 Query End:499 Subject Strand: NEGATIVE Subject Start: 14 Subject End: 286 ......................................................................................................................................................................................................................................tccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacg-gaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatg--aaaat--ctaaaatttccacgcgattatccaaaaattgca................. ......................................................................................................................................................................................................................................tccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccaccttttatgtttccataaaagctacgtcactgtggcactttcagaattactccggcctat-caaagacttagccattttatccgccgtaatgcgcaaagcacatccctagacttatttttttgtctagcacaaacactactggcatcacagccagtgctagcatggactttcctcatgataaaattgcaatatttaccacgcgattatccaaaaattgca................. HSP 2 e-value: 1.0E-9 bit: 65.9 Len: 77 Query Start:119 Query End:195 Subject Strand: NEGATIVE Subject Start: 325 Subject End: 401 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. ......................................................................................................................tatgcaattttctataagccatgttttgttccaaaggcgactaggtaccaccttttttgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. Subject: NC_008024_MGAS10750_Spy1460_MGAS10750_Spy1461|Methyltransferase:Cel l division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1397176,1397619]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_008023_MGAS2096_Spy1373_MGAS2096_Spy1374|Methyltransferase:Cell division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1338520,1338963]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_008022_MGAS10270_Spy1468_MGAS10270_Spy1469|Methyltransferase:Cel l division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1413978,1414421]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_008021_MGAS9429_Spy1347_MGAS9429_Spy1348|methyltransferase:cell division initiation protein|NEGATIVE:NEGATIVE|[1314678,1315121]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_007297_M5005_Spy_1351_M5005_Spy_1352|methyltransferase:cell division initiation protein|NEGATIVE:NEGATIVE|[1320078,1320521]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_007296_M28_Spy1392_M28_Spy1393|methyltransferase:cell division initiation protein DivIVA|NEGATIVE:NEGATIVE|[1389732,1390175]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_006086_M6_Spy1397_M6_Spy1398|Methyltransferase:Cell division initiation protein|NEGATIVE:NEGATIVE|[1392201,1392644]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_004606_SPs0475_SPs0476|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[507839,508282]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: NEGATIVE Subject Start: 9 Subject End: 274 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: NEGATIVE Subject Start: 317 Subject End: 391 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_004070_SpyM3_1386_SpyM3_1387|putative N6-adenine-specific DNA methylase:hypothetical protein|NEGATIVE:NEGATIVE|[1388604,1389047]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_003485_spyM18_1656_spyM18_1658|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1378153,1378596]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_002737_SPy1644_SPy1646|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1365118,1365561]|444 HSP 1 e-value: 9.0E-54 bit: 212.0 Len: 269 Query Start:232 Query End:500 Subject Strand: POSITIVE Subject Start: 171 Subject End: 436 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagccctttcagtcgccgtaacggaaaaatccgtccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcatgaaaatctaaaatttccacgcgattatccaaaaattgcac................ .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccattttttacgtttccataaaaactacgtcactgtggcactttcagaagtactttggcatatcaaacgacttagccatttccttcgccgtaatgtgtatgcacatccctaggcttatgtgttcgcctagcacaatcactactggcatcacagccagtgctagcatggactttcctcat---agtattaaaatactacgcgattatccaaaaattgcac................ HSP 2 e-value: 1.0E-22 bit: 109.0 Len: 75 Query Start:119 Query End:193 Subject Strand: POSITIVE Subject Start: 54 Subject End: 128 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... ......................................................................................................................tatgcaattttctataagccatgttttgttccggaaataactaggaattatttccttcgataatcatctgtctac................................................................................................................................................................................................................................................................................................................................... Subject: NC_007432_SAK_0373_SAK_0374|DivIVA domain protein:hypothetical protein|POSITIVE:POSITIVE|[321731,322199]|469 HSP 1 e-value: 1.0E-34 bit: 149.0 Len: 131 Query Start:232 Query End:362 Subject Strand: NEGATIVE Subject Start: 151 Subject End: 281 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagcccttt.......................................................................................................................................................... .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccactttctagatttccctagaaactacgtcactgtggcactttcaaagctattcaggcatatccaaagacttagcccttt.......................................................................................................................................................... HSP 2 e-value: 3.0E-29 bit: 131.0 Len: 74 Query Start:387 Query End:460 Subject Strand: NEGATIVE Subject Start: 51 Subject End: 124 ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcat........................................................ ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttattttttcgcctagcacaaacactactggcatcacagccagtgctagcatggactttcctcat........................................................ HSP 3 e-value: 2.0E-14 bit: 81.8 Len: 77 Query Start:119 Query End:195 Subject Strand: NEGATIVE Subject Start: 320 Subject End: 396 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. ......................................................................................................................tatgcaattttctataagccatgttttgttccagaaacaactaggaattgtttctttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. Subject: NC_004368_gbs0291_gbs0292|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[313590,314058]|469 HSP 1 e-value: 1.0E-34 bit: 149.0 Len: 131 Query Start:232 Query End:362 Subject Strand: NEGATIVE Subject Start: 151 Subject End: 281 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagcccttt.......................................................................................................................................................... .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccactttctagatttccctagaaactacgtcactgtggcactttcaaagctattcaggcatatccaaagacttagcccttt.......................................................................................................................................................... HSP 2 e-value: 3.0E-29 bit: 131.0 Len: 74 Query Start:387 Query End:460 Subject Strand: NEGATIVE Subject Start: 51 Subject End: 124 ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcat........................................................ ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttattttttcgcctagcacaaacactactggcatcacagccagtgctagcatggactttcctcat........................................................ HSP 3 e-value: 2.0E-14 bit: 81.8 Len: 77 Query Start:119 Query End:195 Subject Strand: NEGATIVE Subject Start: 320 Subject End: 396 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. ......................................................................................................................tatgcaattttctataagccatgttttgttccagaaacaactaggaattgtttctttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. Subject: NC_004116_SAG0302_SAG0303|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[311650,312118]|469 HSP 1 e-value: 1.0E-34 bit: 149.0 Len: 131 Query Start:232 Query End:362 Subject Strand: NEGATIVE Subject Start: 151 Subject End: 281 .......................................................................................................................................................................................................................................ccatgccccgaccaaagtttgggttgctagcttgaggggtttaccgcgttccacttcttctgtttccaaaagaactacgtcactgtggcactttcagacctaatcagacatatccaaagacttagcccttt.......................................................................................................................................................... .......................................................................................................................................................................................................................................ccatgcccctaccaaagtttgggttgctagcttgaggggtttaccgcgttccactttctagatttccctagaaactacgtcactgtggcactttcaaagctattcaggcatatccaaagacttagcccttt.......................................................................................................................................................... HSP 2 e-value: 3.0E-29 bit: 131.0 Len: 74 Query Start:387 Query End:460 Subject Strand: NEGATIVE Subject Start: 51 Subject End: 124 ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttatttcttcgcctagcacaaacactaccggcatcacagccagtgctagcatggactttcctcat........................................................ ..................................................................................................................................................................................................................................................................................................................................................................................................tccctaggcttattttttcgcctagcacaaacactactggcatcacagccagtgctagcatggactttcctcat........................................................ HSP 3 e-value: 2.0E-14 bit: 81.8 Len: 77 Query Start:119 Query End:195 Subject Strand: NEGATIVE Subject Start: 320 Subject End: 396 ......................................................................................................................tatgcaattttctgtaagccatgttttgttccggaaatgactaggtaccatttccttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. ......................................................................................................................tatgcaattttctataagccatgttttgttccagaaacaactaggaattgtttctttcgataatcatctgtctactg................................................................................................................................................................................................................................................................................................................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCCUAGCCUCAGCCUAUAUGCAAUUUUCUGUAAGCCAUGUUUUGUUCCGGAAAUGACUAGGUACCAUUUCCUUCGAUAAUCAUCUGUCUACUGUUUCCAGUCAGAGUGCUAUGUUCGCUUCCACGCACUCCAUGCCCCGACCAAAGUUUGGGUUGCUAGCUUGAGGGGUUUACCGCGUUCCACUUCUUCUGUUUCCAAAAGAACUACGUCACUGUGGCACUUUCAGACCUAAUCAGACAUAUCCAAAGACUUAGCCCUUUCAGUCGCCGUAACGGAAAAAUCCGUCCCUAGGCUUAUUUCUUCGCCUAGCACAAACACUACCGGCAUCACAGCCAGUGCUAGCAUGGACUUUCCUCAUGAAAAUCUAAAAUUUCCACGCGAUUAUCCAAAAAUUGCACU ...................(((((((((.....................(((((((.........)))))))...(((((((.((((...((((....))))))))(((........(((.....(((((....((((.(((....))).)))).(((.(((.((((((((....((((.....(((((.((....)).)))))..))))..(((.(((.......).))....)))................)))))))).))).((((..(((((....)))))..((((((..........))))))............)))).....))).))))).))).((((.((((.....)))).)))).......)))..)))))))..))))))))).. (-88.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGCAAUUUUUGGAUAAUCGCGUGGAAAUUUUAGAUUUUCAUGAGGAAAGUCCAUGCUAGCACUGGCUGUGAUGCCGGUAGUGUUUGUGCUAGGCGAAGAAAUAAGCCUAGGGACGGAUUUUUCCGUUACGGCGACUGAAAGGGCUAAGUCUUUGGAUAUGUCUGAUUAGGUCUGAAAGUGCCACAGUGACGUAGUUCUUUUGGAAACAGAAGAAGUGGAACGCGGUAAACCCCUCAAGCUAGCAACCCAAACUUUGGUCGGGGCAUGGAGUGCGUGGAAGCGAACAUAGCACUCUGACUGGAAACAGUAGACAGAUGAUUAUCGAAGGAAAUGGUACCUAGUCAUUUCCGGAACAAAACAUGGCUUACAGAAAAUUGCAUAUAGGCUGAGGCUAGGAC .(((((((((((.(((((((.((((((((((...))))))))))......(((..(((((((.(((((......)))))..((((((...((((((..(....)..)))))).((((((....))))))..(((.(((....(((((.((((...(((....))))))).)))))...)))))).........((.((((((((....)))))))).))))))))..............))))))).(((..((....))..)))...((((((((((........))).))))))).((((....)))).)))....)))))))...((((((((.......))))))))....................)))))))))))...(((....)))..... (-132.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 705 39 1 231 193 NC_009009:SSA_1856|5end_hypothetical_protein_1852208..1852438_NEGATIVE 418 169 122 71 18 NC_009009:SSA_0620|5end_hypothetical_protein_612325..612555_NEGATIVE 379 138 92 204 164 NC_009009:SSA_0102|5end_Kinase,_putative_104035..104265_POSITIVE 374 129 82 61 19 NC_009009:SSA_1587|5end_hypothetical_protein_1589086..1589316_NEGATIVE 365 177 135 224 181 NC_009009:SSA_1444|5end_Imidazole_glycerol-phosphate_synthase_subunit_hisH,_putative_1447267..1447497_NEGATIVE 362 137 91 178 137 NC_009009:SSA_1487|5end_Conserved_uncharacterized_protein_1494190..1494420_NEGATIVE 350 160 119 179 128 NC_009009:SSA_1778|5end_Segregation_and_condensation_protein_B,_putative_1765711..1765941_NEGATIVE 347 180 131 203 146 NC_009009:SSA_2065|5end_hypothetical_protein_2065743..2065973_NEGATIVE 347 118 71 107 34 NC_009009:SSA_1364|5end_hypothetical_protein_1381297..1381527_NEGATIVE 343 170 125 63 12 NC_009009:SSA_1210|5end_GTP_pyrophosphokinase,_putative_1234655..1234885_NEGATIVE 342 306 263 46 17 NC_009009:SSA_2230|5end_Ribonucleotide_reductase,_class_III,_anaerobic,_putative_2231158..2231388_NEGATIVE 342 128 84 75 33 NC_009009:SSA_2141|5end_Argininosuccinate_lyase,_putative_2142201..2142431_NEGATIVE 339 309 262 206 170 NC_009009:SSA_0334|5end_Diphosphomevalonate_decarboxylase,_putative_327855..328085_POSITIVE 338 159 117 172 126 NC_009009:SSA_0479|5end_CbiQ_protein,_putative_480425..480655_POSITIVE 336 180 131 62 19 NC_009009:SSA_0587|5end_Metal-dependent_amidase/aminoacylase/carboxypeptidase,_putative_578539..578769_POSITIVE 335 348 302 57 9 NC_009009:SSA_1454|5end_hypothetical_protein_1461100..1461330_NEGATIVE 335 199 151 178 119 NC_009009:SSA_1415|5end_Oxidoreductase,_putative_1426508..1426738_NEGATIVE 334 47 2 141 101 NC_009009:SSA_2289|5end_Cadmium_efflux_system_transcriptional_regulator_(ArsR-like),_putative_2291087..2291317_POSITIVE 334 47 2 141 101 NC_009009:SSA_2389|5end_Arsenical_resistance_operon_transcription_repressor,_putative_2032183..2032413_NEGATIVE 334 47 2 111 71 NC_009009:SSA_2387|5end_Arsenical_resistance_operon_transcription_repressor,_putative_2032213..2032443_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 418 169 122 74 21 NC_009009:SSA_0621|3end_SOS_responce_UmuC_protein,_putative_612322..612472_NEGATIVE 392 140 91 127 62 NC_009009:SSA_1960|3end_hypothetical_protein_1956571..1956721_NEGATIVE 368 130 81 109 61 NC_009009:SSA_1230|3end_Conserved_uncharacterized_protein_1252612..1252762_NEGATIVE 355 188 142 143 99 NC_009009:SSA_1182|3end_Gid-like_protein,_putative_1209532..1209682_NEGATIVE 342 128 84 60 18 NC_009009:SSA_2142|3end_Argininosuccinate_synthase,_putative_2142216..2142366_NEGATIVE 341 168 121 98 47 NC_009009:SSA_1680|3end_ABC-type_bacitracin_resistance_protein_A,_permease_component,_putative_1673200..1673350_NEGATIVE 338 159 117 54 8 NC_009009:SSA_0478|3end_Cobalt_transport_protein_cbiN,_putative_480387..480537_POSITIVE 334 47 2 129 89 NC_009009:SSA_2288|3end_Cadmium_resistance_transporter,_putative_2291155..2291305_POSITIVE 334 47 2 129 89 NC_009009:SSA_2026|3end_Cadmium_resistance_transporter,_putative_2032195..2032345_NEGATIVE 322 167 125 101 51 NC_009009:SSA_0187|3end_Competence_protein_ComYD,_putative_190852..191002_POSITIVE 317 52 11 151 108 NC_009009:SSA_2075|3end_Transketolase,_putative_2077113..2077263_NEGATIVE 315 139 99 73 31 NC_009009:SSA_0297|3end_Malolactic_enzyme,_putative_291411..291561_POSITIVE 314 153 118 39 1 NC_009009:SSA_0590|3end_hypothetical_protein_582817..582967_POSITIVE 312 164 126 69 33 NC_009009:SSA_0467|3end_Cobalt-precorrin-6A_synthase_[deacetylating],_putative_471795..471945_POSITIVE 307 349 301 142 94 NC_009009:SSA_0100|3end_DNA_polymerase_I_-_3'-5'_exonuclease_and_polymerase_domains,_putative_103402..103552_POSITIVE 306 165 121 80 30 NC_009009:SSA_2367|3end_ABC-type_cobalt_transport_system,_ATPase_component,_putative_2368845..2368995_NEGATIVE 305 365 322 97 50 NC_009009:SSA_1405|3end_Copper_homeostasis_protein_CutC,_putative_1418758..1418908_NEGATIVE 305 110 65 149 89 NC_009009:SSA_0583|3end_Nucleotidyltransferase,_putative_575679..575829_POSITIVE 303 162 124 39 3 NC_009009:SSA_0197|3end_Dihydropteroate_synthase,_putative_196979..197129_POSITIVE 300 312 284 143 111 NC_009009:SSA_0768|3end_Ribonucleoside-diphosphate_reductase_2,_beta_subunit,_putative_748136..748286_NEGATIVE