Origin IGS: attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgagatttctaacttcatcctgtatccaactctaaatggtgatttttagttggatattcttttgcaaaataacaaacttttgctgacataaaagtgatttttgagctgctcggttttgccgagtcaacgacaagcgaacatttataataatgtagaacaggaaaatgagaagtttaacagaactcagaagattgcgtcggattttggtaagcatattaggaat Mask Tandem Repeat Region ================================================ attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Find is-nt database================================================ Query_seq: SSA_1820:SSA_1821|SSA_1820:SSA_1821:hypothetical protein:Acetyltransferase (N-acetylase of ribosomal proteins), putative:<-<-:1809913..1810136 224 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1820:SSA_1821|SSA_1820:SSA_1821:hypothetical protein:Acetyltransferase (N-acetylase of ribosomal proteins), putative:<-<-:1809913..1810136 224 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1820:SSA_1821|SSA_1820:SSA_1821:hypothetical protein:Acetyltransferase (N-acetylase of ribosomal proteins), putative:<-<-:1809913..1810136 224 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 1 End: 189 M P N M L T K I R R N L L S S V K L L I F L F Y I I I N V R L S L T R Q N R A A Q K S L L C Q Q K F V I L Q K N I Q L K ................................... Protein_Len: 41 Strand: - Start: 1 End: 123 N R I H K G F D S A I K Q T R N F K E N E Q E V N N Y I N A Q R Q S P L V S C S M ..................................................................................................... attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_1820:SSA_1821|SSA_1820:SSA_1821:hypothetical protein:Acetyltransferase (N-acetylase of ribosomal proteins), putative:<-<-:1809913..1810136 224 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Intra-Species Hit: Count: 1 Min: 1 Max: 224 Len: 224 Subject: NC_009009_SSA_1820_SSA_1821|hypothetical protein:Acetyltransferase (N-acetylase of ribosomal proteins), putative|NEGATIVE:NEGATIVE|[1809913,1810136]|224 HSP 1 e-value: 1.0E-124 bit: 444.0 Len: 224 Query Start:1 Query End:224 Subject Strand: POSITIVE Subject Start: 1 Subject End: 224 attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat attcctaatatgcttaccaaaatccgacgcaatcttctgagttctgttaaacttctcattttcctgttctacattattataaatgttcgcttgtcgttgactcggcaaaaccgagcagctcaaaaatcacttttatgtcagcaaaagtttgttattttgcaaaagaatatccaactaaaaatcaccatttagagttggatacaggatgaagttagaaatctcat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUCCUAAUAUGCUUACCAAAAUCCGACGCAAUCUUCUGAGUUCUGUUAAACUUCUCAUUUUCCUGUUCUACAUUAUUAUAAAUGUUCGCUUGUCGUUGACUCGGCAAAACCGAGCAGCUCAAAAAUCACUUUUAUGUCAGCAAAAGUUUGUUAUUUUGCAAAAGAAUAUCCAACUAAAAAUCACCAUUUAGAGUUGGAUACAGGAUGAAGUUAGAAAUCUCAU ..........(((...............)))......(((((((((....(((((......(((((((((.......((((((.((....(((..((((.(((((.....))))))))).))).....)).))))))...((((((........))))))...)))..((((((((..((((....))))..)))))))))))))).))))))))))..)))). (-46.36) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGAGAUUUCUAACUUCAUCCUGUAUCCAACUCUAAAUGGUGAUUUUUAGUUGGAUAUUCUUUUGCAAAAUAACAAACUUUUGCUGACAUAAAAGUGAUUUUUGAGCUGCUCGGUUUUGCCGAGUCAACGACAAGCGAACAUUUAUAAUAAUGUAGAACAGGAAAAUGAGAAGUUUAACAGAACUCAGAAGAUUGCGUCGGAUUUUGGUAAGCAUAUUAGGAAU .....((((((((.((((((((((((((((((..((((....))))..)))))))))....((((((......((((...(..((........))..)..)))).(((((((((.....))))))........)))...............)))))).)))))...))))..(((((.(((((..(.((........)))..))))).)))))...)))))))) (-49.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 932 130 81 221 172 NC_009009:SSA_1820|5end_hypothetical_protein_1809822..1810052_NEGATIVE 328 119 89 159 126 NC_009009:SSA_0111|5end_30S_ribosomal_protein_S19,_putative_110672..110902_POSITIVE 302 119 72 137 83 NC_009009:SSA_2125|5end_Small_primase-like_protein_(Toprim_domain),_putative_2130019..2130249_NEGATIVE 302 70 23 109 59 NC_009009:SSA_1467|5end_Chorismate_synthase,_putative_1474293..1474523_NEGATIVE 297 109 63 64 1 NC_009009:SSA_0532|5end_Propanediol_utilization_protein_PduB,_putative_528244..528474_POSITIVE 296 121 81 228 173 NC_009009:SSA_1438|5end_Acetyltransferase,_putative_1443612..1443842_NEGATIVE 283 146 102 228 186 NC_009009:SSA_2299|5end_hypothetical_protein_2301545..2301775_NEGATIVE 281 128 84 55 18 NC_009009:SSA_1607|5end_Formamidopyrimidine-DNA_glycosylase,_putative_1613255..1613485_NEGATIVE 277 129 85 223 172 NC_009009:SSA_0647|5end_hypothetical_protein_630998..631228_POSITIVE 277 124 81 187 138 NC_009009:SSA_0239|5end_7,8-dihydro-8-oxoguanine-triphosphatase,_putative_232961..233191_POSITIVE 274 139 91 134 85 NC_009009:SSA_2049|5end_Polyribonucleotide_nucleotidyltransferase,_putative_2052894..2053124_NEGATIVE 272 118 72 136 89 NC_009009:SSA_2120|5end_GTPase,_putative_2121751..2121981_NEGATIVE 272 119 88 198 160 NC_009009:SSA_0469|5end_Precorrin-6B_methylase_2,putative_472310..472540_POSITIVE 268 75 33 105 62 NC_009009:SSA_2013|5end_hypothetical_protein_2013793..2014023_NEGATIVE 268 143 101 205 165 NC_009009:SSA_1374|5end_Multidrug_ABC_transporter,_ATPase_and_permease_components,_putative_1393095..1393325_NEGATIVE 268 63 24 70 31 NC_009009:SSA_1119|5end_Two-component_response_transcriptional_regulator_(CheY-like_receiver_and_AraC-type_DNA-binding_domains),_putative_1144491..1144721_POSITIVE 268 119 74 231 176 NC_009009:SSA_0491|5end_Alpha-ribazole-5'-phosphate_phosphatase,_putative_490961..491191_POSITIVE 268 70 23 123 68 NC_009009:SSA_0040|5end_Phosphoribosylaminoimidazole_carboxylase,_ATPase_subunit,_putative_46308..46538_POSITIVE 267 129 89 198 151 NC_009009:SSA_0839|5end_hypothetical_protein_826751..826981_POSITIVE 265 120 88 203 158 NC_009009:SSA_1600|5end_hypothetical_protein_1606595..1606825_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 119 89 73 40 NC_009009:SSA_0110|3end_50S_ribosomal_protein_L2,_putative_110666..110816_POSITIVE 269 49 11 126 87 NC_009009:SSA_0263|3end_Zinc_metalloproteinase_in_scaA_5'region,_putative_258246..258396_POSITIVE 268 70 23 136 81 NC_009009:SSA_0039|3end_Phosphoribosylaminoimidazole_carboxylase,_catalytic_subunit,_putative_46401..46551_POSITIVE 263 107 63 151 108 NC_009009:SSA_2126|3end_hypothetical_protein_2129985..2130135_NEGATIVE 263 48 2 138 78 NC_009009:SSA_1353|3end_hypothetical_protein_1367974..1368124_NEGATIVE 263 119 87 139 102 NC_009009:SSA_0491|3end_Alpha-ribazole-5'-phosphate_phosphatase,_putative_491613..491763_POSITIVE 258 140 96 61 12 NC_009009:SSA_0475|3end_CbiK_protein,_putative_478570..478720_POSITIVE 257 123 83 58 14 NC_009009:SSA_1946|3end_Oligopeptide_transport_system_permease_protein,_putative_1937698..1937848_NEGATIVE 256 46 3 52 4 NC_009009:SSA_1069|3end_Lipoprotein_signal_peptidase,_putative_1088838..1088988_POSITIVE 253 140 101 51 8 NC_009009:SSA_2335|3end_D-Ala-teichoic_acid_biosynthesis_protein,_putative_2338027..2338177_NEGATIVE 253 119 71 48 4 NC_009009:SSA_1780|3end_Tyrosine_recombinase_xerD,_putative_1766416..1766566_NEGATIVE 252 120 88 74 37 NC_009009:SSA_1231|3end_hypothetical_protein_1253649..1253799_NEGATIVE 251 70 21 97 61 NC_009009:SSA_0651|3end_hypothetical_protein_634897..635047_POSITIVE 249 140 94 66 19 NC_009009:SSA_1410|3end_DTDP-4-keto-6-deoxyglucose-3,5-epimerase,_putative_1422493..1422643_NEGATIVE 249 98 51 147 94 NC_009009:SSA_0462|3end_ABC-type_multidrug_transport_system_(phospholipid,_LPS,_lipid_A_and_drug_exporter),_ATPase_and_permease_components,_putative_466652..466802_POSITIVE 245 120 89 38 1 NC_009009:SSA_1452|3end_Second_subunit_of_major_exonuclease,_putative_1456415..1456565_NEGATIVE 244 39 2 116 83 NC_009009:SSA_2299|3end_hypothetical_protein_2300655..2300805_NEGATIVE 244 124 85 49 1 NC_009009:SSA_2094|3end_Conserved_uncharacterized_protein_2097990..2098140_NEGATIVE 244 121 85 89 52 NC_009009:SSA_1899|3end_hypothetical_protein_1894443..1894593_NEGATIVE 244 69 22 114 59 NC_009009:SSA_1338|3end_hypothetical_protein_1351200..1351350_NEGATIVE