Origin IGS: cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cggaatgaatgtatctaagcagtaaattttcctgattgatagaatactatcgttgaaacaatcttaattacatcaatcaaattgcttgctctggcaggctttttttgttatgataatgatgtcttcagggcagggtgagattcccgaccggcggtaatttttggattggaatttttttctccatgtcaaatcaagtccgcgagcgcaagctgatgtggtgagattccacaaccgacagtatagtctggatgggagaagacgagaggcagtgaagcattcttgccgattaatctttatttaagggctgagccttatactaaataatcattagctgacaagccttttttcagtggcctttccggcttctctaatttttataaattggaggaacctgtg Mask Tandem Repeat Region ================================================ cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Find is-nt database================================================ Query_seq: SSA_1773:SSA_1774|SSA_1773:SSA_1774:hypothetical protein:RRNA methylase, putative:<-<-:1760183..1760578 396 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1773:SSA_1774|SSA_1773:SSA_1774:hypothetical protein:RRNA methylase, putative:<-<-:1760183..1760578 396 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1773:SSA_1774|SSA_1773:SSA_1774:hypothetical protein:RRNA methylase, putative:<-<-:1760183..1760578 396 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 110 End: 208 ............................................................................................................. M G K N A S L P L V F S H P D Y T V G C G I S P H Q L A L A D L I ............................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 100 End: 321 ................................................................................................... M K I N R Q E C F T A S R L L P S R L Y C R L W N L T T S A C A R G L D L T W R K K F Q S K N Y R R S G I S P C P E D I ........................................................................... Protein_Len: 46 Strand: - Start: 99 End: 236 .................................................................................................. I F I L R C S H K V A E R R R G D L S Y Q R N H F R V V D A Q A R P S S K V H L F F N W D M ................................................................................................................................................................ cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 283 End: 312 ..........................................................................................................................................................................................................................................................................................CTTGCTCTGGCAGGCTTTTTTTGTTATGAT.................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -6.8 Tail_Score: -5.89921 Start: 298 End: 313 Full_Region: tacatcaatcaaatt gcttgc tctg gcaggc tttttttgttatgat .........................................................................................................................................................................................................................................................................................................gcttgctctggcaggc................................................................................... Find igs database================================================ Query_seq: SSA_1773:SSA_1774|SSA_1773:SSA_1774:hypothetical protein:RRNA methylase, putative:<-<-:1760183..1760578 396 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Intra-Species Hit: Count: 1 Min: 1 Max: 396 Len: 396 Subject: NC_009009_SSA_1773_SSA_1774|hypothetical protein:RRNA methylase, putative|NEGATIVE:NEGATIVE|[1760183,1760578]|396 HSP 1 e-value: 0.0 bit: 702.0 Len: 396 Query Start:1 Query End:396 Subject Strand: POSITIVE Subject Start: 1 Subject End: 396 cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagnnnnnnnttccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacnnnnnnngcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg cacaggttcctccaatttataaaaattagagaagccggaaaggccactgaaaaaaggcttgtcagctaatgattatttagtataaggctcagcccttaaataaagattaatcggcaagaatgcttcactgcctctcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggacttgatttgacatggagaaaaaaattccaatccaaaaattaccgccggtcgggaatctcaccctgccctgaagacatcattatcataacaaaaaaagcctgccagagcaagcaatttgattgatgtaattaagattgtttcaacgatagtattctatcaatcaggaaaatttactgcttagatacattcattccg Inter-species Hit: Count: 19 Min: 135 Max: 277 Len: 143 Subject: smit_SMT_328_SMT_329|23S rRNA methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[264924,265379]|456 HSP 1 e-value: 8.0E-32 bit: 139.0 Len: 70 Query Start:135 Query End:204 Subject Strand: NEGATIVE Subject Start: 223 Subject End: 292 ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:242 Query End:276 Subject Strand: NEGATIVE Subject Start: 64 Subject End: 98 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagac........................................................................................................................ .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagac........................................................................................................................ Subject: NC_008533_SPD_0436_SPD_0437|TrmH family RNA methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[440916,441373]|458 HSP 1 e-value: 8.0E-32 bit: 139.0 Len: 70 Query Start:135 Query End:204 Subject Strand: NEGATIVE Subject Start: 223 Subject End: 292 ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:242 Query End:276 Subject Strand: NEGATIVE Subject Start: 64 Subject End: 98 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagac........................................................................................................................ .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagac........................................................................................................................ Subject: NC_003098_spr0432_spr0433|rRNA methylase:hypothetical protein|POSITIVE:POSITIVE|[433386,433828]|443 HSP 1 e-value: 8.0E-32 bit: 139.0 Len: 70 Query Start:135 Query End:204 Subject Strand: NEGATIVE Subject Start: 223 Subject End: 292 ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ......................................................................................................................................tcgtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:242 Query End:276 Subject Strand: NEGATIVE Subject Start: 64 Subject End: 98 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagac........................................................................................................................ .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagac........................................................................................................................ Subject: NC_004350_SMU.1703c_SMU.1704|hypothetical protein:hypothetical protein|NEGATIVE:POSITIVE|[1614997,1615454]|458 HSP 1 e-value: 8.0E-26 bit: 119.0 Len: 68 Query Start:137 Query End:204 Subject Strand: POSITIVE Subject Start: 120 Subject End: 187 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctcccatccagactttactgtcggttgtggaatttcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 2.0E-11 bit: 71.9 Len: 36 Query Start:242 Query End:277 Subject Strand: POSITIVE Subject Start: 291 Subject End: 326 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... Subject: NC_007432_SAK_1436_SAK_1437|hypothetical protein:sortase family protein, putative|NEGATIVE:NEGATIVE|[1418343,1418625]|283 HSP 1 e-value: 3.0E-25 bit: 117.0 Len: 67 Query Start:137 Query End:203 Subject Strand: POSITIVE Subject Start: 74 Subject End: 140 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggact................................................................................................................................................................................................. ........................................................................................................................................gtcttctcccatccagactttactgtcggttgtggaatctcaccacatcagctttcgctcgcggact................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 39 Query Start:239 Query End:277 Subject Strand: POSITIVE Subject Start: 174 Subject End: 212 ..............................................................................................................................................................................................................................................attaccgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... ..............................................................................................................................................................................................................................................attaccgccggtcgggaattacaccctgccctgaagaca....................................................................................................................... Subject: NC_004368_gbs1473_gbs1474|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1522813,1523113]|301 HSP 1 e-value: 3.0E-25 bit: 117.0 Len: 67 Query Start:137 Query End:203 Subject Strand: POSITIVE Subject Start: 74 Subject End: 140 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggact................................................................................................................................................................................................. ........................................................................................................................................gtcttctcccatccagactttactgtcggttgtggaatctcaccacatcagctttcgctcgcggact................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 39 Query Start:239 Query End:277 Subject Strand: POSITIVE Subject Start: 174 Subject End: 212 ..............................................................................................................................................................................................................................................attaccgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... ..............................................................................................................................................................................................................................................attaccgccggtcgggaattacaccctgccctgaagaca....................................................................................................................... Subject: NC_004116_SAG1403_SAG1404|hypothetical protein:cell wall surface anchor family protein|NEGATIVE:NEGATIVE|[1411665,1411964]|300 HSP 1 e-value: 3.0E-25 bit: 117.0 Len: 67 Query Start:137 Query End:203 Subject Strand: POSITIVE Subject Start: 73 Subject End: 139 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggact................................................................................................................................................................................................. ........................................................................................................................................gtcttctcccatccagactttactgtcggttgtggaatctcaccacatcagctttcgctcgcggact................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 39 Query Start:239 Query End:277 Subject Strand: POSITIVE Subject Start: 173 Subject End: 211 ..............................................................................................................................................................................................................................................attaccgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... ..............................................................................................................................................................................................................................................attaccgccggtcgggaattacaccctgccctgaagaca....................................................................................................................... Subject: NC_008024_MGAS10750_Spy0306_MGAS10750_Spy0307|23S rRNA methyltransferase:Riboflavin transporter|POSITIVE:POSITIVE|[317568,317877]|310 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 161 Subject End: 228 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_008023_MGAS2096_Spy0329_MGAS2096_Spy0330|23S rRNA methyltransferase:Riboflavin transporter|POSITIVE:POSITIVE|[324586,324896]|311 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 229 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_008022_MGAS10270_Spy0307_MGAS10270_Spy0308|23S rRNA methyltransferase:Riboflavin transporter|POSITIVE:POSITIVE|[321257,321565]|309 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 160 Subject End: 227 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 145 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_008021_MGAS9429_Spy0311_MGAS9429_Spy0312|23S rRNA methyltransferase:riboflavin transporter|POSITIVE:POSITIVE|[323660,323970]|311 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 229 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_007297_M5005_Spy_0312_M5005_Spy_0313|23S rRNA methyltransferase:riboflavin transporter|POSITIVE:POSITIVE|[319672,319981]|310 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 161 Subject End: 228 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_007296_M28_Spy0301_M28_Spy0302|23S rRNA methyltransferase:riboflavin transporter|POSITIVE:POSITIVE|[320570,320880]|311 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 162 Subject End: 229 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_006086_M6_Spy0338_M6_Spy0339|23S rRNA methyltransferase:Riboflavin transporter|POSITIVE:POSITIVE|[349118,349427]|310 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 161 Subject End: 228 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_004606_SPs1587_SPs1588|hypothetical protein:putative rRNA methylase|NEGATIVE:NEGATIVE|[1586819,1587184]|366 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: POSITIVE Subject Start: 140 Subject End: 207 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: POSITIVE Subject Start: 222 Subject End: 257 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_004070_SpyM3_0271_SpyM3_0272|putative RNA methyltransferase:hypothetical protein|POSITIVE:POSITIVE|[309561,309869]|309 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 160 Subject End: 227 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 110 Subject End: 145 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_002737_SPy0371_SPy0373|putative rRNA methylase:hypothetical protein|POSITIVE:POSITIVE|[319078,319387]|310 HSP 1 e-value: 5.0E-21 bit: 103.0 Len: 68 Query Start:137 Query End:204 Subject Strand: NEGATIVE Subject Start: 161 Subject End: 228 ........................................................................................................................................gtcttctcccatccagactatactgtcggttgtggaatctcaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ ........................................................................................................................................gtcttctctcatccagactatactgtcggttgtggagttacaccacatcagcttgcgctcgcggactt................................................................................................................................................................................................ HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 36 Query Start:242 Query End:277 Subject Strand: NEGATIVE Subject Start: 112 Subject End: 147 .................................................................................................................................................................................................................................................accgccggtcgggaatctcaccctgccctgaagaca....................................................................................................................... .................................................................................................................................................................................................................................................accgccggtcgggaatcacaccctgccctgaagaca....................................................................................................................... Subject: NC_003454_FN1504_FN1505|Nickel-binding protein:6,7-dimethyl-8-ribityllumazine synthase|NEGATIVE:POSITIVE|[10106,10535]|430 HSP 1 e-value: 1.0E-9 bit: 65.9 Len: 49 Query Start:138 Query End:186 Subject Strand: NEGATIVE Subject Start: 303 Subject End: 351 .........................................................................................................................................tcttctcccatccagactatactgtcggttgtggaatctcaccacatca.................................................................................................................................................................................................................. .........................................................................................................................................tcttctcccatccagactctactgtcggttttggaatttcaccaaatca.................................................................................................................................................................................................................. HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 36 Query Start:241 Query End:276 Subject Strand: NEGATIVE Subject Start: 245 Subject End: 280 ................................................................................................................................................................................................................................................taccgccggtcgggaatctcaccctgccctgaagac........................................................................................................................ ................................................................................................................................................................................................................................................taccaccggtcgggaatttcaccctgccctgaagac........................................................................................................................ Subject: NC_008593_NT01CX_0715_NT01CX_0716|riboflavin biosynthesis protein RibD:Predicted metal-binding protein|NEGATIVE:POSITIVE|[2406701,2407315]|615 HSP 1 e-value: 2.0E-8 bit: 61.9 Len: 47 Query Start:140 Query End:186 Subject Strand: POSITIVE Subject Start: 276 Subject End: 322 ...........................................................................................................................................ttctcccatccagactatactgtcggttgtggaatctcaccacatca.................................................................................................................................................................................................................. ...........................................................................................................................................ttctcccatccagactttactgtcggtcttggaatctcaccaaatca.................................................................................................................................................................................................................. HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:240 Query End:274 Subject Strand: POSITIVE Subject Start: 346 Subject End: 380 ...............................................................................................................................................................................................................................................ttaccgccggtcgggaatctcaccctgccctgaag.......................................................................................................................... ...............................................................................................................................................................................................................................................ttaccgccggtcgggaatttcaccctgccctgaag.......................................................................................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCGUCUUCUCCCAUCCAGACUAUACUGUCGGUUGUGGAAUCUCACCACAUCAGCUUGCGCUCGCGGACUUGAUUUGACAUGGAGAAAAAAAUUCCAAUCCAAAAAUUACCGCCGGUCGGGAAUCUCACCCUGCCCUGAAGACA ..((((((.........(((......)))(((.(((((.(((((((.....(((....))).((((...((((((....(((((((.....)))...)))).)))))))))).))).)))).)).)))...)))..)))))). (-36.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUCUUCAGGGCAGGGUGAGAUUCCCGACCGGCGGUAAUUUUUGGAUUGGAAUUUUUUUCUCCAUGUCAAAUCAAGUCCGCGAGCGCAAGCUGAUGUGGUGAGAUUCCACAACCGACAGUAUAGUCUGGAUGGGAGAAGACGA .((((((..((..(((.......)))..)).((((.....(((((..((((.........))))..)))))......)))).(((....)))..(((((.......))))).(((.(((......)))..)))..)))))).. (-44.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 66 23 231 187 NC_009009:SSA_2239|5end_Conserved_uncharacterized_cytosolic_protein_2237233..2237463_NEGATIVE 366 80 42 36 1 NC_009009:SSA_0448|5end_Excinuclease_ATPase_subunit_A,_putative_446480..446710_POSITIVE 352 67 24 173 116 NC_009009:SSA_1659|5end_ABC-type_antimicrobial_peptide_transport_system,_permease_component,_putative_1656819..1657049_NEGATIVE 342 85 44 202 151 NC_009009:SSA_1879|5end_Glycyl-tRNA_synthetase_beta_chain,_putative_1874262..1874492_NEGATIVE 335 66 25 225 178 NC_009009:SSA_2363|5end_Phosphoglycolate_phosphatase,_putative_2365632..2365862_POSITIVE 334 41 4 203 155 NC_009009:SSA_1940|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III,_putative_1927129..1927359_NEGATIVE 332 46 5 58 15 NC_009009:SSA_0629|5end_hypothetical_protein_618967..619197_POSITIVE 327 67 24 172 115 NC_009009:SSA_1680|5end_ABC-type_bacitracin_resistance_protein_A,_permease_component,_putative_1675200..1675430_NEGATIVE 326 64 25 43 7 NC_009009:SSA_0914|5end_Phenylalanyl-tRNA_synthetase_beta_chain,_putative_924952..925182_POSITIVE 325 55 13 185 138 NC_009009:SSA_0299|5end_hypothetical_protein_292759..292989_POSITIVE 321 97 51 162 118 NC_009009:SSA_1323|5end_hypothetical_protein_1340450..1340680_NEGATIVE 320 70 25 208 164 NC_009009:SSA_2042|5end_hypothetical_protein_2047073..2047303_NEGATIVE 314 70 33 52 1 NC_009009:SSA_0781|5end_Mannose-6-phosphate_isomerase,_putative_765373..765603_POSITIVE 313 63 24 183 133 NC_009009:SSA_1398|5end_Membrane_protein,_hemolysin_III-like,_putative_1410881..1411111_POSITIVE 313 78 50 176 137 NC_009009:SSA_0585|5end_hypothetical_protein_576937..577167_POSITIVE 313 84 41 210 151 NC_009009:SSA_0211|5end_hypothetical_protein_208041..208271_POSITIVE 310 135 97 231 182 NC_009009:SSA_2071|5end_hypothetical_protein_2074766..2074996_NEGATIVE 310 100 52 213 167 NC_009009:SSA_0883|5end_Glycerate_kinase,_putative_877560..877790_POSITIVE 307 54 11 50 4 NC_009009:SSA_0464|5end_Cobalamin_biosynthesis_protein_cobD,_putative_468225..468455_POSITIVE 304 68 24 231 177 NC_009009:SSA_1847|5end_RRNA_methyltransferase_RsmB,_putative_1842004..1842234_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 43 6 139 99 NC_009009:SSA_1822|3end_hypothetical_protein_1810871..1811021_NEGATIVE 332 46 5 71 28 NC_009009:SSA_0628|3end_Fe-S-cluster_redox_enzyme/radical_SAM_enzyme,_Cfr_family,_putative_619060..619210_POSITIVE 325 68 30 64 10 NC_009009:SSA_0486|3end_Uroporphyrinogen-III_synthase,_putative_487442..487592_POSITIVE 307 54 11 48 2 NC_009009:SSA_0463|3end_Cobyrinic_acid_A,C-diamide_synthase,_putative_468303..468453_POSITIVE 302 137 93 142 97 NC_009009:SSA_2340|3end_hypothetical_protein_2341539..2341689_NEGATIVE 302 50 2 70 26 NC_009009:SSA_2190|3end_33_kDa_chaperonin,_putative_2192118..2192268_POSITIVE 300 43 1 146 98 NC_009009:SSA_1375|3end_Multidrug_ABC_transporter,_ATPase_and_permease_components,_putative_1393085..1393235_NEGATIVE 295 140 99 36 4 NC_009009:SSA_0066|3end_hypothetical_protein_68095..68245_POSITIVE 290 60 11 147 102 NC_009009:SSA_0621|3end_SOS_responce_UmuC_protein,_putative_612322..612472_NEGATIVE 286 136 91 64 17 NC_009009:SSA_1411|3end_Glucose-1-phosphate_thymidylytransferase,_putative_1423091..1423241_NEGATIVE 283 133 92 136 87 NC_009009:SSA_1650|3end_3-Ketoacyl-ACP_reductase,_putative_1648896..1649046_NEGATIVE 283 95 51 147 96 NC_009009:SSA_0414|3end_hypothetical_protein_410569..410719_POSITIVE 282 70 27 149 111 NC_009009:SSA_1440|3end_Phosphoribosyl-ATP_pyrophosphohydrolase,_putative_1444407..1444557_NEGATIVE 278 69 24 151 105 NC_009009:SSA_1630|3end_hypothetical_protein_1631508..1631658_POSITIVE 278 58 30 39 9 NC_009009:SSA_0660|3end_Cell_division_protein_DivIVA,_putative_644672..644822_POSITIVE 276 61 27 144 106 NC_009009:SSA_0314|3end_Nucleoside-diphosphate-sugar_epimerase,_putative_308967..309117_NEGATIVE 275 57 18 71 25 NC_009009:SSA_1248|3end_hypothetical_protein_1272355..1272505_NEGATIVE 274 139 94 145 107 NC_009009:SSA_1960|3end_hypothetical_protein_1956571..1956721_NEGATIVE 273 70 25 151 108 NC_009009:SSA_1748|3end_Inorganic_pyrophosphatase/exopolyphosphatase,_putative_1733543..1733693_NEGATIVE 273 84 42 75 33 NC_009009:SSA_0057|3end_Phosphotransferase_system_sugar-specific_EII_component,_putative_61246..61396_POSITIVE